TLS OnlineQUESTION ID:1
The following are certain facts regarding bioremediation:
A. Biodegradable plastics are made using polyhydroxy alkanoates (PHAs) such as polyhydroxybutyrate (PHB).
B. Pseudomonas putida F1 bacterial strain is involved in degradation of aromatic hydrocarbon.
C. The bacterium Deinococcus radiodurans consume and digest toluene and ionic mercury from highly radioactive nuclear waste.
D. Bioaugmentation is a process of improving the microorganisms already existing in the system for degradation of xenobiotic compound.
Which one of the following combination of above statements is correct?
QUESTION ID:2
An unknown peptide was isolated from the leaf of a medicinal plant and purified to homogeneity. The peptide did not yield any sequence when subjected to Edman degradation. However, tryptic digest of the peptide produced a unique sequence. The mass of the intact peptide was found to be 18 Da less than that obtained from the trypsin treated sample. The possible interpretation of the above experimental results could be that the
A. the N-terminus of the peptide was blocked by acetylation or methylation.
B. the peptide was cyclic and contained at least one internal arginine or lysine residue.
C. the peptide was cyclic and contained a lysine or arginine residue at the C terminus.
D. the peptide was cyclized by peptide bond formation between α- amino group and α-carboxyl group.
Which of the above statements is true?
QUESTION ID:3
A gene is regulated by a novel transcription factor. The following techniques may be used to identify the cis- regulatory element in the 1 kb promoter sequence of the gene where the novel transcription factor binds:
A. Bioinformatics analysis.
B. Cell based reporter assay.
C. S 1 nuclease assay
D. Electrophoretic mobility shift assay.
E. DNAse-I foot-printing analysis
Which one of the following can help to identify the cis element?
QUESTION ID:4
A protein D is encoded by a gene, which is 5 Kb long and has three Hind III restriction enzyme sites. The first one is 0.5 Kb from the transcription start site, the second one is 2.5 Kb from the first site and the third one is 0.5 Kb internal to the stop codon. The second site is polymorphic. In order to find out whether fetal cells contain the normal or the mutated gene, total genomic DNA from fetal cells was isolated, completely digested with Hind III, separated in an agarose gel, transferred to membrane and detected by a probe against the region between the second and third restriction site. Which one of the following band patterns will be obtained if the fetal cell is heterozygous?
QUESTION ID:5
Given below are the experimental protocols to find out the exact location of repetitive DNA sequence in mitotic chromosome by FISH (fluorescence in situ hybridization). Which one of the protocols will give the correct result?
QUESTION ID:6
At 25°C values of [θ]222, the mean residue ellipticity at 222 nm, are - 33,000 and -3,000 deg cm2 dmol-1 for a polypeptide existing in α- helical (α) and β-structure (β), respectively. If this polypeptide undergoes a two-state heat-induced α→β transition, and a value of [θ]222 = -18,000 deg cm2 dmol-1 is observed at 60°C, then this observation leads to the conclusion that the α helix conversion to β- structure is:
QUESTION ID:7
An EEG was recorded and its power spectrum analyses were done in rats with implant electrode for a long time. The power of the EEG waves decreased two months after electrode implantation. This observation may be due to the following:
A. Glial cells accumulate surrounding exposed tips of electrodes.
B. Degeneration of neurons occur surrounding the electrode tips due metal ion deposition.
C. Coating of electrodes are destroyed with time.
D. The microsocket becomes loose time.
Which one of the following is true?