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QUESTION ID:1
Which one of the following statements is true for trends of the dissolved oxygen (DO) and biological oxygen demand in water stream receivingpollutants from point source
QUESTION ID:2
Following are the graphical representation of various hypothesis proposed for explaining the possible relationship between the species richnesson (X) axis and community services on (Y) axis.Which of the following is the correct match between the graphical representation and the hypothesis

QUESTION ID:3
Agobacterium Ti plasmid vectors are used to generate transgenic plants. The following are examples of vir gene encoded proteins that are important for transfer of T-DNA in to plants.
A- vir E, single stranded DNA binding protein
B- virD2 that generates T-strands
C- vir A that sense plant phenolic compounds
D- vir F which directs T complex proteins for destruction in proteasome
Which one of the following combination of the proteins functions inside the plant cell?
QUESTION ID:4
Researcher is investigating structural changes in protein by following tryptophan fluorescence and by Circular dichorism. Fluorescence and CD spectraof pure protein were obtaining the absence of any treatment (a), in the presence of 0.5 molar urea (b), upon adding acrylamide, a quencher of tryptophan(c) and upon heating (d) the data are shown below.Which one of the following statements is correct?

QUESTION ID:5
Polynucleotide kinase (PNK) is frequently used for radio labeling DNA or RNA by phosphorylating 5’ end of non-phosphorylated polynucleotide gene.Which of the following statements about PNK is NOT true?
QUESTION ID:6
A gene encoding for protein X was cloned in an expression vector under the T7 RNA polymerase promoter and lac operator. Cells were incubated bythe addition of 1 mM IPTG at 370 c for 6 h. Cells were lysed and fractionised into insoluble bodies. Which one of the following strategies would you use to express protein X in the soluble fraction (cell free supernatant)?
QUESTION ID:7
Engineering of metabolic pathways in plants can be achieved by introduction and overexpression of appropriate candidate gene(s) using transgenictechnology. The figure given below represents a biochemical pathway in plants where a precursor molecule ‘A’ is converted in to products ‘T’ and ‘X’through a series of enzymatic reactions. Enzymes 1- 5 are involved in this pathway. Scientists attempted to increase the levels of ‘X’ by introducing additional copy of the gene for enzyme ‘5’ under transcriptional control of a strong constitutive promoter. However, the developed transgenic plants did not display a proportionate increase in the level of ‘X’.The following statements were proposed for explaining the above results: A- Enzyme ‘4’ has greater affinity for D than enzyme ‘3’B- Feedback inhibition of enzyme ‘5’ by compound X C- Substrate limitation for enzyme ‘5’Which of the above statement could represent probable reasons for NOT obtaining proportionate increase in the amount of ‘X’ in the transgenic plants?

QUESTION ID:8
A single copy homozygous transgenic plant containing the transgene 'A ' for fungal resistance was subsequently re-transformed with another gene' B' for conforming resistance to salt stress. The selection marker genes used for both the transformation experiment were different.Transgenic plant obtained following the retransformation experiment were screened for salt stress resistance and single copy event were identified by southern hybridization. These single copy event were self - pollinated. In the event of the two T-DNA (containing the A and B transgenes) getting integrated in unlinked location in all transgenic plants, the phenotypic ratios among the T1 progeny would be:
QUESTION ID:9
You are inserting a gene of 2 kb length in to a vector of 3kb to make a GST fusion protein the gene is being inserted at the E. coli site and the insert hasa Hind III site 500bp downstream of the first codon. You are screening for the clone with the correct orientation by a restriction digestion of the plasmidusing Hind III plus Bam HI (H+P) and Hind iii plus PSTI (H+P) the map of the relevant region of the vector is shown belowWhich of the plasmid shown above represents the clone in the correct orientation?

QUESTION ID:10
The frequency of M-N blood types in a population of 6129 individuals is as follows:The frequency of LN allele in this population is

QUESTION ID:11
Mayfair genes (HYPOTHETICAL) consist of a superfamily of transcription factors. They are found in 4 clusters in mammals; in 2 clusters in insects; andin a single cluster in an ancestor to insects. These data consistent with all of the following explanations EXCEPT:
QUESTION ID:12
Fluorescence recovery after photo bleaching (FRAP) is a method to estimate the diffusion of molecules in a membrane. Fluorescently labelled in a membrane such asi) a receptor tagged with green fluorescent protein (GFP)ii) a receptor labelled with GFP which interacts with cytoskeletoniii) a labelled lipidiv) a labelled protein that binds to the membrane surfaceare photo bleached and the recovery profiles ( a-d) were obtained to estimate their diffusion coefficientsThe following data were obtained.Which one of the combination is correct?
