Previous Year Questions

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TLS Online TPP Program

QUESTION ID:1

To achieve a best resolution using a fluorescence microscope, what combination of wavelength of emitted light (λ), refractive index and the angle (2θ) by which light enters into the microscope would be the best choice for the user: 

QUESTION ID:2

Given below are spatial and temporal techniques (column I) used to detect brain activity (column II)  Select the correct set of combination 

QUESTION ID:3

Following observations are made regarding a peptide sequence.

- The peptide is inert to Ellman’s reagent. However, on reacting with beta-mercaptoethanol, the peptide gives a positive Ellman’s test.

- The peptide sequence gives a broad minimum around 211 nm in the CD spectrum.

- With increasing concentration of the peptide, the melting temperature of the peptide increases.

- On treating the peptide with D2O, half the total number of amides get exchanged.

Which one of the following statements is correct?

QUESTION ID:4

If a metabolically active cell is challenged with 55Fe radioisotope label, which of the following proteins CANNOT be detected by autoradiography?

QUESTION ID:5

Diagnosis of influenza virus infections can be done using some of the following techniques:

A. Western blot and Southern blot

B. Northern blot and western blot

C. ELISA and RT-PCR

D. PCR and electron microscopy

Choose the combination of techniques that correctly lists the detection methods. 

QUESTION ID:6

From the following statements:

(A) Coloured images can be obtained by transmission electron microscopy by fluorescent labelling of the specimen

(B) Scanning electron microscopy requires sectioning of the sample

(C) Confocal microscopy uses optical methods to obtain images from a specific focal plane and excludes light from other planes

(D) Differential-interference microscopy relies on interference between polarized light due to differences in the refractive index of the object and surrounding medium

(E) Visualization in epifluorescence microscopy requires staining by heavy metal atoms

Choose the combination with two correct and one incorrect statements.