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QUESTION ID:1
Construction of knockout mice may be performed using the Cre-LoxP system. Eventually, the Cre recombinase of the bacteriophage P1 mediates sitespecific recombination at a 34 bp sequence, lox P. From the following statements, choose the INCORRECT event:
QUESTION ID:2
Monoclonal antibodies can be modified for better research and therapeutic applications. Several such approaches are mentioned below (Column A) along with their possible applications (Column B).
Column A | Column B | ||
i. | Binding site of the original mouse mAb are placed onto the Fe regions of human antibodies | a | Abzymes |
ii. | Antibodies are modified by conjugation to toxins designed to kill cells to which the antibody will bind | b | Reduce side effect of xenogenic antibodies in immunotherapy |
iii. | Generation of mAb that specifically bind and stabilize the transition state of a chemical reaction | c | Immunotoxins |
Which one of the following options represents a correct combination of terms in Column A and Column B?
QUESTION ID:3
Given below is a table comprising various terms associated with plant tissue culture in Column A and Column B.
Column A | Column B | ||
A | Auxin | i | Embryogenesis |
B | Protoplast culture | ii | 6-Furfuryl amino purine |
C | Cytokinin | iii | Pectinase and Cellulase |
D | Microspore culture | iv | Indole-3-acetic acid |
Which one of the following options represents the most appropriate match between all the terms of Column A and Column B?
QUESTION ID:4
In an attempt to increase the yield of a commercially important enzyme from natural isolate several strategies were adopted as follows:
A. Genome was selectively modified to increase yield.
B. Reappraisal of culture requirements of the modified organism to increase yield.
C. Induced mutants were screened and selected for organism synthesizing improved levels of the enzyme.
D. Organism was genetically modified, so that it produces a factor that enhances stability of the enzyme.
Which one of the following options represents strategies that are appropriate for the purpose?
QUESTION ID:5
Given below are names of techniques (Column A) and their characteristic features/applications (Column B):
| Column A | Column B | ||
| A | Hybridoma technology | (i) | Separation of technology proteins according to charge |
| B | MALDI-TOF | (ii) | Identification of protein complexes in cells |
| C | Ion-exchange chromatography | (iii) | Production of identical antibodies |
| D | Co-immunoprecipitation | (iv) | Determination of molecular weight of proteins and/or peptides |
Which one of the following represents a correct match between Column A and Column B:
QUESTION ID:6
Three proteinsBlm 1, Blm 2, Blm 3 were shown to be involved in repair of DNA double strand breaks. A chromatin immunoprecipitation experiment was performed for the three proteins. The pattern of results obtained is shown below:
Based on the above figure, choose the option that correctly interprets the data.
QUESTION ID:7
The most important property of any microscope is its resolution (D). Which one of the following wavelengths (nm) would be used to achieve the best resolution using a light microscope with lenses having numerical aperture (NA) of 1.4?
QUESTION ID:8
Detailed NMR spectra of a 20-residue peptide were recorded using a 600 MHz instrument. If the peptide adopts an αhelical conformation, which one of the following statements is correct?
QUESTION ID:9
Given below are names of statistical distribution (Column I) and their characteristic features (Column II)
Column I | Column II | ||
A | Binomial distribution | i | Each observation represents one of two outcomes (success or failure) |
B | Poisson distribution | ii | Probability that is symmetric about the mean |
C | Normal distribution | iii | Probability of a given number of events happening in a fixed interval of time |
Which one of the following represents a correct match between columns I and II?
QUESTION ID:10
Highly purified peptides Pl, P2 and P3 were subjected to MALDI mass spectral analysis. The following observations were made: P1: Showed a m/z of 16 more than the expected value. P2: Showed a m/z of 80 more than the expected value. MS/MS spectra of the peptide resulted in a precursor ion with m/z 98 less than the expected m/z. P3: Showed a m/z that was double the expected value. [Note: z = + 1 for all the mass spectra.]
Which one of the options given below comprises all correct interpretations?