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Previous Year Questions

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TLS Online TPP Program

QUESTION ID:1

An oligopeptide is subjected to amino acid analysis and found to have the following composition
Tyr, Phe, Asp, Val, Arg, Met
The following statements represents/outline/list the results obtained after analysis:
A. The above oligopeptide is subjected to N-terminal Edman degradation, revealing Tyrosine residue.
B. Trypsin treatment did not affect the peptide.
C. Cyanogen bromide treatment yielded a dipeptide, tetrapeptide, and free Arginine.
D. Chymotrypsin treatment yielded dipeptide and tetrapeptide. The composition of tetrapeptide was Valine, Arginine, and Methionine.
Based on above observations, what is the CORRECT sequence of heptapeptide?

QUESTION ID:2

Apoplast phloem loading is determined by the cellular location and transport function of the membrane-bound proteins. Following are certain statements regarding these proteins
A. SWEETs are the sugar transporter proteins and are capable of transporting only sucrose and not glucose.
B. Double mutants of Arabidopsis SWEET11 and SWEET12 results in carbohydrate accumulation in the source leaves and slower export of the
photoassimilates.
C. H+-symport mechanism loads sucrose or polyols into Sieve Element (SE)/Companion Cell (CC) complexes.
D. Several clades of sucrose/H+ symporters (SUTs. or SUCs) are localized to plasma membranes of minor vein SE/CCs and participate in apoplastic loading.
Which one of the following combinations is correct?

QUESTION ID:3

The statements below attempt to describe a few characteristics of Alu repeats found in the human genome
A. Alu elements are a class of short interspersed elements (SINEs)
B. SINEs are autonomous transposons
C. Alu repeat originated from cDNA copies of ?SL RNA
D. Alu repeats have a rela tively high AT content
E. They are preferentially located in the gene-poor G chromosome bands.
Which one of the following options shows combination of all correct statements?

QUESTION ID:4

Synchronous cultures of MCF7 breast cancer cells were grown and treated  with the following:
(i) buffer (plate was named MCF7),
(ii)_an inhibitor of Cyclin D (plate was
named MCF7.1), and
(iii) siRNA designed against Cyclin B1 (plate was named MCF7.
Following this, the cells were stained and sorted using flow cytometry. The relative amount of DNA in each of the three phases of the cell cycle (G1, S, G2/M in arbitrary units) were plotted against the number of cells, as
shown below.

Which one of the following options correctly represents all the cell cycle states of MCF7, MCF7.1 and MCF7.2?

QUESTION ID:5

A new strain of bacteria was created by introducing an artificial operon, to allow bacterial cells to grow in the presence of iron. The Fe++ operon consisted of genes that made the cells capable of tolerating increased iron. For efficient functioning of this operon, the following desirable features were considered.
Which one of the following can be used to develop this operon?

QUESTION ID:6

Which one of the following Newick trees represents the correct relationship between apes.

QUESTION ID:7

The structure and process of formation of different antigens in blood ABO system are given in the following statements:
A. Galactose is added to the terminal of H-antigen lby a transferase expressed in individuals with type A blood.
B. The B antigen is formed by a transferase expressed in individuals with type B blood which adds a terminal N-acetylgalactosamine to H-antigen.
c. The H-antigen is formed by fucose transrerase that adds a terminal fucose to its precursor.
D. The H-antigen is the precursor of both the A- and B- antigens and it is the blood group antigen in persons of type O blood.
Which one of the following options represents the correct combination of statements?

QUESTION ID:8

The following statements are suggested regarding the principle and uses of positron emission tomography (PET):
A The PET scanner is a positron ray detector.
B. The PET scanner cannot determine the location of collision between positron and electron in the brain.
C. The typical PET aciivation studies can measure the absolute metabolic activity of brain.
D. In PET, a radioactive isotope is introduced into blood as 'tracer' that rapidly decays by emitting a positron from their atomic nuclei.
Which one of the following options represents the combination of correct statement(s)?

QUESTION ID:9

In a study comparing different plant communities (A to D) across a landscape, the following data were obtained:
Which one of the following options represents the pair of communities with highest similarity value when Sorenson's coefficient is used?

QUESTION ID:10

The table below lists phylogenetic reconstruction methods and the description of these methods, which includes both algorithmic and optimality-based methods or criteria\


Method (Column X)


Description (Column Y)

A.

Maximum parsimony

i.

Tree length, that is sum of branch lengths, often estimated by least squares.

B.

Minimum evolution

ii.

Minimum number of changes, minimised over ancestral states

C.

Bayesian

iii.

Cluster algorithms to arrive at a single tree.

D.

Neighbour Joining

iv.

Posterior probability, calculated by integrating it over branch lengths and substitution parameters

Select the option that best matches the tree reconstruction method (Column X) with its correct description in Column Y

QUESTION ID:11

The table below represents some protein modifications in Column X and their functions in Column Y

Column X

Column Y

Protein modification

Function

a. Palmitoylation

i. Protein degradation

b. polySUMOylation

ii. Membrane anchoring

c. Glycosylphosphatidylinositol

iii. Lysosomal targeting

d. Mannose-6-phosphate

iv. Protein folding

Which one of the following options represents all correct matches between Column X and Column Y?

QUESTION ID:12

The central rod domain of keratin protein is 300 amino acids in length. What is the approximate length (in A) of the rod domain if the peptide consists of
i) distorted a-helix
ii) true a-helix
iii) anti-parallel j3-sheet

QUESTION ID:13

In an experiment using nude mice, the population is divided into two groups, A and B. Group A mice are injected with T cells from normal mice and Group B mice are left untreated. Both the groups are then immunized with LPS. Which one of the following statements regarding antibody production in groups A and B is most likely to be true?

QUESTION ID:14

In birds and mammals, 3rd, 4th and 6th aortic arches persist in adult animals with some structural modifications. Which of the following modified organization is correct?

QUESTION ID:15

Unknown antigens can be detected or measured by a sandwich ELISA whereas Elispot assays measure the number of cells capable of secreting particular molecules such as cytokines which is considered as antigens.
Which of the following is true for both assays?

QUESTION ID:16

Iron (Fe) is taken up by cells via receptor-mediated endocytosis utilizing transferrin and transferrin receptor. In a cell line with a mutation in the transferrin receptor that is unable to interact with transferrin at pH (4-6),
which one of the following steps will be first affected in this pathway?

QUESTION ID:17

Consider a highly diverse community of closely related species of lizards which has evolved in a short period of time and that occupies different ecological niches in peninsular India. What type of speciation process can
explain the above pattern?

QUESTION ID:18

Defending a territory is energetically expensive and animals should invest in this only if it is profitable A sunbird species is dependent on a plant species that contains nectar-rich flowers, making it an important resource for the sunbird. Males may defend territories containing these plants against other males, while allowing females to access the flowers. In this way they keep competitors out and get access to the females.
The columns below (P to S) represent characteristics of the resources and their variants (i and ii)

Column P

Column Q

Column R

Column S

Abundance of flowers in the habitat

Distribution of flowers in the habitat

Nectar depletion rate of flowers

Nectar renewal rate of flowers

i. High

i. Uniform

i. High

i. High

ii. Low

ii. Patchy

ii. Low

ii. Low

From the given options, choose the combination of plant characteristics that makes defending a territory most profitable for males

QUESTION ID:19

Extracellular matrix comprises of various proteins and polysaccharides that assemble into an organized meshwork. This associates with the cells that produce them. Given below are a few statements regarding different components of the matrix.
A. Collagen is the major protein of the extracellular matrix and is a long, and triple-stranded helical structure.
B. Hyaluronan which is produced in large quantities during wound healing is a type of gIycosaminogIycan (GAG) that contains sulfated sugar and is covalently linked to the core protein.
C. Syndecans are plasma membrane proteoglycans that interact with the actin cytoskeleton and signaling molecules of the cell cortex.
D. Decorin is a small
Which one of the following options represents INCORRECT statement/s?

QUESTION ID:20

The characteristic features and causes of different heart sounds during a cardiac cycle of humans are given in the following statements
A. The second heart sound is loud and sharp when the diastolic pressure is decreased in the aorta or pulmonary artery.
B. Sudden closure of atrioventricular (AV) valves at the start of ventricular systole caused vibration that produces first heart sound.
c. The second heart sound is caused by the vibration associated with the closure of aortic and pulmonary valves after the end of ventricular systole.
D. The first heart sound is soft when heart rate is low as the ventricles are well filled with blood and the leaflets of AV valves float together before systole.
Which one of the following options represents the combination of all correct statements?

QUESTION ID:21

Selected human diseases and their vectors are listed below:

Column X

Column Y

Disease

Vector

A. Chikungunya

i. Aedes mosquito

B. Lyme disease

ii. Anopheles mosquito

C. Lymphatic filariasis

iii. Aquatic snails

D. Schistosomiasis

iv. Ticks

Which one of the following options represents the correct match between column X and column Y?

QUESTION ID:22

You have purified an enzyme that has a molecular weight of 60 kDa. You run this protein on an SOS-PAGE gel and get the following results
Which of the following statements is valid for the quaternary structure of this enzyme?

QUESTION ID:23

Figure A represents the sites for Ncol (N) in a binary vector pCSIR2023. The vector is 16500 bp in size. Figure B represents the fragments observed when the vector is either digested with Ncol (N) or double digested with Hindlll and Ncol (H+N). The intensity of fluorescence of the 453 bp fragment is double that of the 516 bp fragment
Which one of the following statements regarding the numbers and location(s) of Hindlll site is correct?

QUESTION ID:24

Which one of the following statements about change in temperature with elevation is correct?

QUESTION ID:25

The sensory nerve fibers (Column X) and the sensory receptors connected to different sensory nerves (Column Y) are given below

Column X

Column Y

Sensory nerve fibre

Sensory receptor

A. Ia

i. Muscle spindle, flower spray ending

B. Ib

ii. Pain and cold receptor

C. II

iii. Muscle spindle, annulo-spiral ending

D. III

iv. Golgi tendon organ

Which of the following options represents the correct match between column X and column Y?

QUESTION ID:26

In DNA foot-printing,
A The DNA is labelled by random priming so that the entire DNA is labelled and one does not miss out any region that binds with the given protein.
B. The DNA is end-labelled so that the bands get organized from higher to lower size after electrophoresis and autoradiography.
C. A sequencing polyacrylamide gel is used to resolve all the fragments distinctly.
D. A higher concentra1ion of agarose gel is used to resolve the finer bands.
Which one of the following options has the combination of all correct statements?

QUESTION ID:27

Given below are statements about development in d ifferent model organisms
A. Xenopus egg has yolk and hence undergoes meroblastic cleavage.
B. Embryonically transcribed beta-catenin in the blastomeres of sea urchin embryos regulates the autonomous specification of micromeres.
C. The sodium pump activity in the trophoblast helps in the formation of blastocoel of a mammalian blastocyst.
D. Prevention of tubal pregnancy is one of the major functions of zona pellucida in humans.
Which combination of the statements is true?

QUESTION ID:28

Epistasis is observed between different genes in flower color of sweet pea. A red variety on selfing yields red:white in ratio 9:7. The precursors and intermediates give white color. The following biochemical pathways were
proposed for the above observations:
Which one of the options represents the correct pathway(s) that explains the observations?

QUESTION ID:29

Match the names of scientists (column X) with the ecosystem concepts (column Y) they are known for:

Column X

Column Y

A. Charles Elton

i. trophic dynamics

B. A G Tansley

ii. the term ecosystem

C. A J Lotka

iii. pyramid structure of feeding relationships in ecosystems

D. Raymond Lindeman

iv. a thermodynamic view of the ecosystem

QUESTION ID:30

In C. elegans, PAR proteins segregate at the cell cortex in the zygote to establish cell polarity. This is dependent on the regulation of the cortical actin cytoskeleton by RhoA. An investigator sought to directly inhibit actin polymerization to analyze the impact of this inhibition on PAR protein localization. Which one of the following chemicals would be the most suitable

QUESTION ID:31

This is a hypothetical example. In a plant, a single gene governs flower color. The wild type color is red, while the mutant is white. It was demonstrated that insertion of a transposable element caused the white phenotype. In a PCR test, a set of primers outside the site of insertion is used to amplify the genomic DNA and the PCR products are resolved by Agarose gel electrophoresis. A geneticist made a cross between two plants. 30 progeny from the cross was analyzed by PCR. Each lane in the gel below represents analysis of one progeny
Based on the above, which one of the following statements is correct?

QUESTION ID:32

The amino acid sequence of tetrapeptides (P, Q, R) is shown below.
P) Asp-Gly-Asp-Ser Q) Gly-Ser-Arg-Gly R) Gly-Lys-Arg-Ala
i. Calculate the net charge on the above tetrapeptides at pH 7.0
ii. If the mixture of the above tetrapeptides is separated on a cationexchange
column at pH 7.0, which tetrapeptide will elute last?
Choose the correct answer given below.

QUESTION ID:33

The figure below depicts the reflectance curves of different features found in an urban ecosyste
Which one of the options below correctly identifies the reflectance curves?

QUESTION ID:34

What is the V0!Vmax ratio for an enzymatic reaction when [SJ= 3 Km and 9 Km, respectively?

QUESTION ID:35

By expressing the EGF-like ligand LIN-3, the c. e/egans anchor cell (AC) directly triggers vulval development. LIN-3 acts at a distance and has a graded action. The levels of LIN-3 can be controlled by using various
genetic techniques.

Which one of the following options is a correct match between column I and column II?

QUESTION ID:36

The following statements describe the developmental processes during different modes of reproduction in angiosperms:
A. In double fertilization, one sperm fuses with the egg and other with the central cell to form the zygote and endosperm, respectively.
B. In sporophytic apomixis, a diploid cell gives rise to the next generation thereby maintaining the maternal genotype.
c. In gametophytic apomixis, a reduced egg cell forms apomictic embryo through parthenogenesis.
D. In pseudogamy, the functional endosperm is formed without fertilization

QUESTION ID:37

Researcher plans to study protein trafficking into the endoplasmic reticulum (ER). For this purpose, they plan different experimental conditions shown below:
A. The cytosol is mixed with mRNA that codes for a secreted protein, followed by wesiern blotting with antibodies against secreted protein.
B. The cytosol is mixed with mRNA that codes for a secreted protein and rough microsomes, followed by western blotting with antibodies against secreted protein.
C. The cytosol is mixed with mRNA that codes for a secreted protein and rough microsomes followed by protease treatment. Subsequently, western blotting with antibodies against secreted protein is done
Consider that mRNA that codes for a secreted protein is added in abundant amount, which experimental control/s would be the best to confirm the polypeptide entry into the ER?

QUESTION ID:38

A mutant strain of E.coli having defects in one of the DNA repair pathways was identified. To identify the pathways where mutation occurred, aresearcher looks at the following parameters and identified the defect to be
in base excision repair pathway
A. Topoisomerase 11 enzyme activity
B. AP endonuclease activity
c. Expression ot mutL and muts
D. DNA glycosylase activity
E. DNA ligase activity
Based on the changes in which of the above parameters, this conclusion can be drawn?

QUESTION ID:39

The graph given below is based on the optimal foraging theory. If the Y axis represents "Cumulative Resource Intake" following tihe law of diminishing
returns, what do T and T' stand for?

QUESTION ID:40

The graph below depicts the net change in forest cover in four regions (AD) between 1990 and 2020, according to the FAO report - 'The State of World's Forests 2020'.
Which one of the following options correctly identifies these regions?

QUESTION ID:41

The Cre-LoxP system was used to knock-out the p53 gene from the mice lungs. An immunoblot analysis was carried out as shown below

The following assumptions were made
A. The LoxP system did not work since the recombinase was not functional.
B. The Lox P sites were introduced under a promoter specific for lungs.
C. The tissue-specified promoter selected was of the prostate gland.
D. The mice died because of being knocked out of p53.
E. The knocked-out mice developed mutagen-induced tumours in their prostate gland more rapidly than in their lungs
Which one of the following is correct combination of above assumption?