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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Following statements were made about the characteristics of cyclin proteins:

A. Synthesis of M-cyclin is dependent on the cyclin mRNA that is newly transcribed after every cycle.

B. Destruction of M-cyclin toward the end of mitosis is driven by ubiquitin independent proteolytic system.

C. Gl cyclins can be activated by mitogenic factors.

D. Retinoblastoma (Rb) is a key target of the activated cyclin D-Cdk 4/6 complex.

E. While cyclin Al expression is ubiquitous, cyclin A2 expression is restricted to the germ cell lineages.

Which one of the following options contains a combination of all correct statements?

QUESTION ID:2

Following statements were made about catalytic introns:


A. Group I introns may undergo self-splicing by transesterification.


B. Group II introns do not require any factor/protein for autosplicing either in vivo or in vitro


C. Certain introns of both the group I and II classes may contain open reading frames which are translated into protein.


D. Generally, group I introns migrate by DNAmediated mechanisms, whereas group II introns migrate by RNA-mediated mechanisms.


E. Ribonuclease P {RNase P) is essential for bacteria and archaea but not eukaryotes.


Which one of the following combinations represents statements which are all correct?

QUESTION ID:3

A protein found in the golgi compartment was found to be degraded rapidly at mitosis. Golgi extracts (isolated from G1 and M phase cells treated with deubiqutination inhibitors) were treated with glycosidase and western blots done
using antibodies against the protein. The following pattern was obtained.
Identify the statement that CANNOT be made based on the data.

QUESTION ID:4

Following statements were made about regulation of eukaryotic gene expression.

A. It is usually regulated at the level of initiation of transcription by altering the chromatin architecture.

B. The eukaryotic genome is divided into domains by insulator elements.

C. A chromatin remodeling complex binds to the promoter of a gene in sequence specific manner.

D. Architectural proteins regulate gene expression by promoting DNA bending.

E. The chromatin remodeling complex can alter nucleosomal architecture, but cannot displace them.

The option with all the correct statements is

QUESTION ID:5

Contents in Column I and II are with respect to bacterial transcriptional regulation


Which one of the options below correctly matches contents in column I and Column II?

QUESTION ID:6

Which one of the following statements about eukaryotic RNA processing is INCORRECT?

QUESTION ID:7

The following statements are made with reference to the fact that tRNAs are known to possess tRNA their sequence.

A. RNA polymerase Ill utilizes TTP as one of the substrates.

B. Any other RNA polymerase, RNA polymerase Ill also utilizes UTP but when the RNA pol Ill reaches a looped structure it binds to S-adenosylmethionine to methylate C-5 position of the incorporated U to result in a thymine in a co- transcriptional manner.

C. During transcription of tRNA genes at the designated positions, DNA polymerase replaces RNA polymerase Ill to incorporate T in the tRNA transcript

D. A specific methyltransferase utilizes a methyl group donor to post transcriptionally modify the specific u residues into T residues.

E. Uracil to thymine conversion occurs in a large number of tRNAs in the TVC loop.

The option with all the correct statements is

QUESTION ID:8

Leishmanai major, the causative protozoan paratiste of cutaneous leishmaniasis, resides and multiplies within the phagosomes of

macrophages. Resistance to the infection correlated verv well with the development of a Thl response. In order to prove this hypothesis,

three groups of BALB/c mice were taken: control group, IFNyC-/ -) group and IL-4 (-/-)group. All the groups were infected with L. Major and then parasite load was meausred in each group after 4 weeks, Which one of the following result will justify the experimental outcome confirming the hypothesis?





QUESTION ID:9

Receptor tyrosine kinases (RTKS) form a large and important class of cell surface receptors whose ligands are soluble or membrane bound protein or peptide hormones.

Which of the following statement is INCORRECT?

QUESTION ID:10

The following statements are made with reference to characteristics of glycosaminoglycans and proteoglycans, which are major constituents of extracellular matrix. Which one of them is INCORRECT?

QUESTION ID:11

Prostate cancer cells were treated with Drug A and Drug B in order to check the efficacy of the drugs in arresting the growth of cells The following results were obtained:

 Which one of the following statements is NOT correct?

QUESTION ID:12

A transgenic mouse was generated for both heavy and light chain specific for MHC molecule H-2K^m so that all the B cells in this mouse therefore had BCRS specific only for H-2K^m and made only anti- H-2K^M specific antibodies. By appropriate breeding, the H-2K^m specific immunoglobulin transgene was introduced into
mice bearing different MHC genotypes H-2K^n and H-2K^m/n. With respect to selection of B-cells within the bone marrow of H-2K^n and H-2k^m/n mice,
which of the following statements is correct?

QUESTION ID:13

Following diagram displays the MHC haplotype of a heterozygous H-2k/d mouse:
How many types of MHC molecules will be expressed on activated macrophage (M<p) and a normal fibroblast (NF)?

QUESTION ID:14

In C. elegans the SKN-1 protein controls t he fate of the EMS blastomere which generates the posterior pharynx. With reference to the above

which one of the following statements is INCORRECT?

QUESTION ID:15

Maternal effect genes are extremely important in establishing the anterior-posterior polarity of the Drosophila embryo. Mutant phenotypes arise when genes of this family are mutated. The following table enlists genes and phenotypes observed on mutation of these genes but not correctly matched
Which one of the following combinations is correctly matched?

QUESTION ID:16

The hedgehog pathway is extremely important in vertebrate limb development, neural differentiation and facial morpho-genesis. In accordance with the above statement, what would happen in mice that are homozygous for a mutant allele of sonic hedgehog (shh)?

QUESTION ID:17

In Xenopus, the Noggin protein, accomplishes two major functions of the organizer: it induces dorsal ectoderm to form neural tissues, and it dorsalizes mesoderm cells. Which one of the following observations is correct with respect to Noggin?

QUESTION ID:18

Antennapedia protein expresses in the thoracic segment of the fly and not in the head region. However, a dominant mutation of Antennapedia replaces antenna with leg-like structure.

The following statements were made with reference to Antennapedia:

A. In a dominant Antennapedia mutant, the Antennapedia gene is expressed in the head as well as in thorax.

B. In the Antennapedia mutants, the Antennapedia gene is expressed in head region only and thus promotes leg-like structure in head socket

C. In addition to promoting thoracic structures, Antennapedia protein binds to and represses the enhancer of hemothorax and some other genes responsible for antenna specification.

D. Antennapedia has no role in thoracic region specification and thus recessive Antennapedia mutants show no phenotypic effect.

Which one of the following options represents correct statement(s)?

QUESTION ID:19

The development of anthers and male gametophytes is highly conserved among angiosperms. Following are some of the events

associated with pollen development, in random order.

(a) Microsporogenesis in pollen sac to produce a tetrad.

(b) Asymmetric division forming immature pollen grain.

(c) Archespore division.

(d) Division of generative cell to form two sperm cells.

(e) Callase digestion to form free microsperes.

Which of the following option represent the correct series of events during pollen development?

QUESTION ID:20

Following are certain statements regarding C2, c3, c4 and CAM carbon metabolism in plants.
A. In c3 cycle, one molecule of 3- phosphoglycerate formed during carboxylation phase is utilized for the biosynthesis of sugars, fatty acid and amino acids
B. During C2 cycle glycine is transported from peroxisome to mitochondria and glycerate is transported from peroxisome to chloroplast
C. The concentration of CO2 in bundle sheath of C4 plants is several fold lower than the external atmosphere
D. The stomata of CAM plants open at night
Which one of the following combination of statements is correct?

QUESTION ID:21

Following is the list of cellular responese of root hair to bacterial Nod factor and timing of their induction , which may or may not be matched.


Which one of the following is the correct match?

QUESTION ID:22

Plant growth hormone receptors, namely TIR1, GID1, 2011 and PYL1 were knocked out independently in Arabidopsis plant. The resultant plants are named as tir1, gid1, coit and pyll. Which one of the following statements regarding the above Arabidopsis plants is correct?

QUESTION ID:23

Followings are certain statements regarding phytochrome-mediated signal transduction in Arabidopsis:

A. phyA and phyD are photo-stable, while phy, phyc and phyE are photo-labile.

B. Prolonged light exposure and the conversion of PRAto PERA cause phyA concentration to drop.

C. Exposure of light and conversion of PR to PFR cause movement of phytochromes from the cytosol into the nucleus.

D. phyB lacks nuclear localization sequence and depends on transporter protein for nuclear import

Which one of the combinations of above statements is correct?

QUESTION ID:24

The gene encoding for mevalonate-3-kinase isdisrupted in a certain plant. Which one of the following statements concerning the above Plan t is correct?

QUESTION ID:25

Plants perceive effector proteins from pathogen to mount a strong defense response. The following statements were made regarding signal transduction events upon perception of a pathogen.
A. Influx of Ca 2+ and W ions into the cell
B. Influx of K+ and Cl" ions into the ce ll
C. Efflux of Ca 2+ and W ions out of the cell
D. Efflux of K+ and Cl" ions out of the cell
Which one of the following combination of statements is correct?

QUESTION ID:26

The action potential recorded from pace maker tissue (SA/AV node) of mammalian heart is shown in the following diagram:


On the basis of mechanism of generation of action potential in pace maker tissue (SA/AV node), the following statement were proposed from the above figure

A. 'funny' channels are activated at 'b'

B. Outward flow of K+ occurs at 'a'

C. T-ea++ channels are closed at 'c'

D. Fast Na+ channels are opened at 'd'

E. The upward phast at 'd' is largely due to inward movement of Na+

Which one of the following combinations represent correct statements?

QUESTION ID:27

Some metabolic aspects of the Red Blood Cell (RBC) are proposed in the following statements:

A. Synthesis of fatty acids does not occur in the RBC

B. The pentose phosphate pathway is operative in the RBC

C. RBC cannot synthesize reduced glutathione (GSH)

D. RBC does not contain enzymes like adenosine deaminase and pyrimidine nucleotidase

E. NADH-dependent methemoglobin reductase system is present in RBC

Which one of the following combinations is NOT correct?

QUESTION ID:28

Insulin is a heterodimer made up of A and B peptide chains joined by the intra and interchain disulfide bridges formed between amino acid of respective chains as suggested below:
A. A6A 11
B.  A7 B7
C.  A20  B 19
D.   A5      B15
Which one of the following represents the correct disulfide bridges joining A and B chains of insulin hormone?

QUESTION ID:29

Fibrinogen, Factor 1, is a soluble glycoprotein present abundantly in plasma. Following biochemical characteristics are given below about this glycoprotein:

A. It is a dimer of the three polypeptide chains (AaBβ γ)2

B. It is a dimer of two polypeptide chains (AaB β) 2

C. The chains are covalently linked by 29 interand intra-chain disulfide bonds

D. The chains are covalently linked by 19 interand intra-chain disulfide bonds

Which one of the following represents a combination of correct statements?

QUESTION ID:30

Testosterone is a steroid hormone about which a set of statements are given below:

A. It is secreted by leydig cells of testes.

B. It is secreted by Sertoli cells of testes.

C. It inhibits secretion of luteinizing hormone from anterior pituitary.

D. It does not inhibit secretion of follicle-stimulating hormone from anterior pituitary.

Which one of the following combinations represents correct statements about testosterone?

QUESTION ID:31

A scientist synthesized four new chemicals which had mutagenic potential andnamed them as C1, C2, C3 and C4. He tried to analyse the nature of mutationscaused by them and obtained the following results:
Which one of the following answers describes the mutagenic potential of the chemicals?

QUESTION ID:32

Fertilization of gametes containing chromosome with duplications or deletionsoften results in children with disabilities. What is the probabi lity of a couplewhere the male is ka ryotypically normal and the female has a pericentric inversionin heterozygouscondition prod ucing a child with disabilities if crossing over took place within the pericentric inve rsion in 26% of meiotic divisions?

In this case consider that fertilization with a gamete containing chromosomes with duplications or deletion will result in disabilities.

QUESTION ID:33

In D. Melanogaster, traits 'a', 'b' and 'c' result from recessive alleles that are located on one of the autosomes. The resulting F1 females were test crossed and the following progeny (total of 1000) were obtained:
The following conclusions were made from the above data:
a+ b+ c+ 350
a+ b c      25
a  b  c     380
a b+ c      35
a b+ c      100
a b c +       10  
 a + b  c +         85     
a+ b+ c     15
The following conclusions were made from the above data:
A. The order of genes is a b c and the distance between a and b is 8.ScM.
B. The order of genes is a c band the distance between a and c is 8.ScM.
C. The order of genes is b ca and the distance between band c is 21 cM.
D. The order of genes is c b a and the distance between band c is 8.ScM.

QUESTION ID:34

The table given below shows the Lod score values of three different pairs of genes studied for assessing if they are linked pairs


The following conclusions were made from the data given above:


A. For gene pair 1 the probability of the genes being linked is 10 times more likely than them assorting independently.


B. For gene pair 2, the Lod score 2 indicates that the probability of the genes being linked is twice more likely than assorting independently.


C. The genes of pair 1 and 2, can both be considered as linked, while the genes of pair 3 exhibits independent assortment.


D. The genes of pair 3 can be considered as linked.


Which one of the following options represents statement(s) that is/are correct?

QUESTION ID:35

Gene 'A' in mouse is needed for its normal growth [normal size]. Mouse strains with deletion in gene 'A' (Adel) have been developed.
When the following cross is made
..... Adel AxAA ..... .
All progeny {both males and females) are of normal size.
When the Fl, progeny with the following genotypes are crossed: ......
AdelA x AA .... .
50% of the progeny obtained are small in size.
The above observation can be explained by:

QUESTION ID:36

In a mammal, coat colour is governed by gene B, The coat colour is either black or brown, depending on whether the genotype is BB or Bb. It is not known which of these genotypes lead to the black and brown colours. The genotype bb results in albino coat colour. Further, the genotype ce suppresses the expression of coat colour resulting in albino coat colour. An albino male was crossed with a brown female and the resulting progeny had individuals with either black or brown coats. From this observation it can be infered that the genotype of the male and female that were crossed are:

QUESTION ID:37

The diagram below shows the floral diagram of the family Malvaceae. Parts of the diagram have been labe lled from A-D.\




Which of the following combinations represent all correct matches between labels and floral characters?

QUESTION ID:38

The following diagrams {A to D) show characteristic arrangement of stamens within a flower and {i) to {v) enlist families of plants.

  

i. Cucurbitaceae

ii. Fabaceae

iii. Malvaceae

iv. Asteraceae

v. Euphorbiaceae

Which one of the following options has all correct matches between the arrangement of stamens and the families they belong to?

QUESTION ID:39

The figure below shows reptilian skull types (A to C), class of organisms (i to iii) and examples of animals (X to Z). 
Which one of the following options shows correct matches between the three components?

QUESTION ID:40

Column I list names of organisms and column li list stages in life cycle.


Which one of the following options represents all correct matches between the two columns?

QUESTION ID:41

Given below are statements regarding apomixis, i.e. asexual reproduction through seeds

A. Sporophytic apomicts often produce a mix of clonal and sexual progeny

B. In gametophytic apomixis, the unreduced central cell gives rise to apomictic embryo

C. In pseudogamy the endosperm is formed in the absence of fertilization

D. Apomixis can potentially be used to maintain hybrid vigour over many generations

Which one of the following options represents the combination of all correct statements?

QUESTION ID:42

The linkage density in a food web is a function of the connectance and the number of species in it. It is defined as the average number of feeding links per species. Which one of the following would have the highest linkage density?





QUESTION ID:43

Ozone concentration in the atmosphere is measured in Dobson units (Du). An ozone hole is said to have occurred when the concentration ofozone in the region falls below 220 Du. The diagram of the globe below shows the location of three study sites. The table below it showsvalues of ozone concentrations recorded at the sites for two dates in 2009.



QUESTION ID:44

A population of crickets invading a new grassland showed a population growth pattern as shown in the figure


Following is the list of potential interpretations:

A. Environment is damaged due to population overshooting its K

B. The resources did not recover and population dies out

C. Carrying capacity is lowered due to shift in environmental conditions.

Which one of the following options/combination of options can correctly explain the cricket growth pattern?

QUESTION ID:45

Given below are the spectra of plant forms (on the basis of where the plants bear their buds) in\ different biomes (A to C).




Which one of the following options correctly identifies the biomes represented in graphs A to C?

QUESTION ID:46

Given below are growth and survivorship curves.
Select the correct combination of growth curves from figure A and su1v ivorship curves from figure B given above, that would best represent r and K strategies, respectively.

QUESTION ID:47

The following four climatograms depict observation of mean temperature and rainfall for four locations. The solid lines depict temperature while the dashed line depicts rainfall.


Given below are five biomes:

I. Desert

II. Savannah

Ill. Tropical rain forest

IV. Mediterranean

V. Conifer ecosystem

Which of the following combinations correctly matches the climtograms to the biomes?

QUESTION ID:48

A tree built using BLAST cannot be used to infer phylogenetic relationships. Given this, which of the following statements is NOT true about trees generated by BLAST?

QUESTION ID:49

Given below are names of scientists and phrases describing their work, which may or may not be matched

Which one of the following options re presents correct matches between the scientist and his/ her work?

QUESTION ID:50

In a population that is in a Hardy-Weinberg equilibrium, 40% of the plants are recessive homozygotes and produce white flowers (WF). If the total number of individuals in the population is 14000 plants, the numbers of homozygous dominant red flowered (RF) plants and heterozygous pink flowered (PF) plants would be:

QUESTION ID:51

You wanted to conduct Miller-Urey experiment and used a simplified apparatus with Tungsten electrodes. You heated the glassware at 5oooc for 3 hours to remove any organic contaminants. Gases NH3, CH4, CO and H2 were introduced followed by generating electric spark. Which of the essential ingredients did you forget to add?

QUESTION ID:52

Given below are the few statements on concepts related to genome evolution.

A. Presence of introns in some chloroplast genes suggests that endosymbiosis (leading to organelle evolution) occurred before loss of introns in prokaryotes and supports the hypothesis that genes originated as interrupted structures.

B. Negative selection is associated with increased rate of nonsynonymous substitutions as compared to synonymous substitutions.

c. Nucleotide substitution rates during evolution can be inferred from divergence of the sequences that are non-functional or neutral.

D. Positive selection is associated with increased rate of nonsynonymous substitutions as compared to synonymous substitutions.

Which one of the following options represents a combination of all INCORRECT statements?

QUESTION ID:53

Growth patterns of two species (grown alone or together) are shown in Figures A and B


Match the growth patterns with the correct type of interaction represented by them

QUESTION ID:54

A group of researchers are testing two new agents, M1 and M2 for their efficacy in selecting transgenic plants. When they performed tissue culture experiments using three explants, A, B, and C without Agrobacterium transformation, and selected the regenerated plants on M1 and M2, the following regene-ration frequencies were obtained.


Based on the above data, which one of the following conclusions is INCORRECT?

QUESTION ID:55

RNA silencing is an important strategy to control viral infection in plants. The following statements were made regarding RNA silencing.

A. It is driven by small interfering RNA (SiRNA) derived from double-stranded form of viral RNA.

B. siRNA requires RISC for it function.

C. Many plant viruses encode proteins that act as suppressors of RNA silencing.

D. Viral P19 protein activates RNA induced silencing complex

Which one of the following combination of statements is correct?

QUESTION ID:56

One curie (Ci) is defined as the qua ntity of radioactive substance having a decay rate of 2.22 X 1012 disintegra tions per minute (dpm). How much of counts per minute (cpm) will be recorded for 100 μCi of 32P when measured in a scintillation counter working at 50% efficiency?

QUESTION ID:57

Given below are a few statements related to plant breeding and genetics:

A. Non-random mating between individuals closely related to each other would tend to promote a decrease in homozygosity at all loci.

B. Quantitative traits are typically influenced by environmental conditions to a greater extent than qualitative traits.

C. The "over dominance theory for explanation of heterosis assumes that in a heterozygote, only one of the alleles plays a functional role.

D. During domestication selected genes/traits

show a decrease in diversity. 

Which one of the following options represents a combination of all INCORRECT statements?

QUESTION ID:58

Given below are a few terms in column A and column B.


Which one of the following options represents all correct matches between terms of Column A and Column B?

QUESTION ID:59

Following are the typical dot plots of cytometric data obtained from peripheral white blood cells:

                     PLOT 1                                                                      PLOT 2

    

R1 in plot 1 shows the selection window of cells which were further

analysed based on immunostaining (plot 2). Analyze the given data and choose

the correct option representing the type of cell population in plot 1 selected and

segregated in various quadrants of plot 2.

QUESTION ID:60

The data from an S1 nuclease mapping experiment for a transcript mfg1 using a 5' –end labelled probe are shown below.



Following interpretations we re made:

The liver RNA is 500 bp in length

The start site of the liver mfg1 transcript is 500 bp downstream of the 5' end of probe.

The kidney makes two mfg 1 transcripts, and the 3' end of one of these is shorter than the other.

Which one of the following options represents the correct combination of the interpretations?

QUESTION ID:61

Given below is a schematic representation of a few restriction sites in the multiplecloning site of a plasmid:
The recognition sequences of the above enzymes and their digestion patterns are shown below:
Which one of the following combinations of enzymes from the vector can be used for cloning a DNA fragment obtained as a Sal I (GTICGAC) - Hind II (GTYT RAC) fragment

QUESTION ID:62

Given below is a schematic representation ofthe T-DNA region of a transgene construct and the Southern blot analysis (using Pst I digested genomic DNA) of five independent transgenic lines (labelled as A to E) developed using theconstruct. The probe used for hybridization is shown as a black box below the construct (UT:Untransformed Plant)


Based on the above data, which one of the following options gives the correct list of single insertion transgenic events?

QUESTION ID:63

Given below are the restriction profiles obtained upon digestion of a 4.8 kb plasmid with four different enzymes (E1, E2, E3 and E4).

E1:   4.8 kb E1+E2 = 500 bp + 4.3 kb

E2 :  4.8 kb+ E2+E3=  400bp+ 4.4 kb

E3  :  4.8 kb+ E3 + E4 =  2 kb+ 2.8 kb

E4 :   4.8 kb+ E1+  E4 = 1.9 kb +2.9 kb

Based on the above information, which one of the following statements is correct?

QUESTION ID:64

The 1H NMR spectrum of 13CH3CI in CDCl3 shows two lines (doublet, 1 :1 intensity} spaced 140 Hz apart. The carbon (13C} NMR spectrum of the same molecule shows four lines (quartet, intensity ratio 1:3:3:1 }. Both 1H and 13c have nuclear
spin quantum numbers I = 1/2.
Based on the above information which one of the following options is correct?

QUESTION ID:65

Given below are a few statistical terms in Column A and their related features and terms in Column B.


Which one of the options given below correctly matches all items of Columns A and B?