Menu

Previous Year Questions

Performance Meter

0%

TLS Online TPP Program

QUESTION ID:1

You wanted to conduct Miller-Urey experiment and used a simplified apparatus with Tungsten electrodes. You heated the glassware at 5oooc for 3 hours to remove any organic contaminants. Gases NH3, CH4, CO and H2 were introduced followed by generating electric spark. Which of the essential ingredients did you forget to add?

QUESTION ID:2

Given below are the few statements on concepts related to genome evolution.

A. Presence of introns in some chloroplast genes suggests that endosymbiosis (leading to organelle evolution) occurred before loss of introns in prokaryotes and supports the hypothesis that genes originated as interrupted structures.

B. Negative selection is associated with increased rate of nonsynonymous substitutions as compared to synonymous substitutions.

c. Nucleotide substitution rates during evolution can be inferred from divergence of the sequences that are non-functional or neutral.

D. Positive selection is associated with increased rate of nonsynonymous substitutions as compared to synonymous substitutions.

Which one of the following options represents a combination of all INCORRECT statements?

QUESTION ID:3

Growth patterns of two species (grown alone or together) are shown in Figures A and B


Match the growth patterns with the correct type of interaction represented by them

QUESTION ID:4

A group of researchers are testing two new agents, M1 and M2 for their efficacy in selecting transgenic plants. When they performed tissue culture experiments using three explants, A, B, and C without Agrobacterium transformation, and selected the regenerated plants on M1 and M2, the following regene-ration frequencies were obtained.


Based on the above data, which one of the following conclusions is INCORRECT?

QUESTION ID:5

RNA silencing is an important strategy to control viral infection in plants. The following statements were made regarding RNA silencing.

A. It is driven by small interfering RNA (SiRNA) derived from double-stranded form of viral RNA.

B. siRNA requires RISC for it function.

C. Many plant viruses encode proteins that act as suppressors of RNA silencing.

D. Viral P19 protein activates RNA induced silencing complex

Which one of the following combination of statements is correct?

QUESTION ID:6

One curie (Ci) is defined as the qua ntity of radioactive substance having a decay rate of 2.22 X 1012 disintegra tions per minute (dpm). How much of counts per minute (cpm) will be recorded for 100 μCi of 32P when measured in a scintillation counter working at 50% efficiency?

QUESTION ID:7

Given below are a few statements related to plant breeding and genetics:

A. Non-random mating between individuals closely related to each other would tend to promote a decrease in homozygosity at all loci.

B. Quantitative traits are typically influenced by environmental conditions to a greater extent than qualitative traits.

C. The "over dominance theory for explanation of heterosis assumes that in a heterozygote, only one of the alleles plays a functional role.

D. During domestication selected genes/traits

show a decrease in diversity. 

Which one of the following options represents a combination of all INCORRECT statements?

QUESTION ID:8

Given below are a few terms in column A and column B.

Column A

Column B

A. Cisgenics(i)Synteny studies
B. Zinc-finger proteins(ii)Linkage disequilibrium
C. Comparative genomics(iii)Site-specific recombination
D. Association mapping(iv)Use of endogenous plant genes and regulatory elements
Which one of the following options represents all correct matches between terms of Column A and Column B?

QUESTION ID:9

Following are the typical dot plots of cytometric data obtained from peripheral white blood cells:

                     PLOT 1                                                                      PLOT 2

    

R1 in plot 1 shows the selection window of cells which were further

analysed based on immunostaining (plot 2). Analyze the given data and choose

the correct option representing the type of cell population in plot 1 selected and

segregated in various quadrants of plot 2.

QUESTION ID:10

The data from an S1 nuclease mapping experiment for a transcript mfg1 using a 5' –end labelled probe are shown below.



Following interpretations we re made:

The liver RNA is 500 bp in length

The start site of the liver mfg1 transcript is 500 bp downstream of the 5' end of probe.

The kidney makes two mfg 1 transcripts, and the 3' end of one of these is shorter than the other.

Which one of the following options represents the correct combination of the interpretations?

QUESTION ID:11

Given below is a schematic representation of a few restriction sites in the multiplecloning site of a plasmid:
The recognition sequences of the above enzymes and their digestion patterns are shown below:
Which one of the following combinations of enzymes from the vector can be used for cloning a DNA fragment obtained as a Sal I (GTICGAC) - Hind II (GTYT RAC) fragment

QUESTION ID:12

Given below is a schematic representation ofthe T-DNA region of a transgene construct and the Southern blot analysis (using Pst I digested genomic DNA) of five independent transgenic lines (labelled as A to E) developed using theconstruct. The probe used for hybridization is shown as a black box below the construct (UT:Untransformed Plant)


Based on the above data, which one of the following options gives the correct list of single insertion transgenic events?

QUESTION ID:13

Given below are the restriction profiles obtained upon digestion of a 4.8 kb plasmid with four different enzymes (E1, E2, E3 and E4).

E1:   4.8 kb E1+E2 = 500 bp + 4.3 kb

E2 :  4.8 kb+ E2+E3=  400bp+ 4.4 kb

E3  :  4.8 kb+ E3 + E4 =  2 kb+ 2.8 kb

E4 :   4.8 kb+ E1+  E4 = 1.9 kb +2.9 kb

Based on the above information, which one of the following statements is correct?

QUESTION ID:14

The 1H NMR spectrum of 13CH3CI in CDCl3 shows two lines (doublet, 1 :1 intensity} spaced 140 Hz apart. The carbon (13C} NMR spectrum of the same molecule shows four lines (quartet, intensity ratio 1:3:3:1 }. Both 1H and 13c have nuclear
spin quantum numbers I = 1/2.
Based on the above information which one of the following options is correct?

QUESTION ID:15

Given below are a few statistical terms in Column A and their related features and terms in Column B.

Column A

Column B

A. Standard deviation(i)Measure of relative variability of given populations.
B. Coefficient of Variation(ii)Used to make inferences about population means.
C. Chi-square test(iii)Positive square root of population variance.
D. t-Test(iv)Test hypothesis related to categorical data from inheritance studies.
Which one of the options given below correctly matches all items of Columns A and B?