Previous Year Questions (PYQ) | 2016-June | CSIR-NET Life Sciences

QUESTION ID:1

In a typical gene cloning experiment, by mistake a researcher introduced the DNA of interest within ampicillin resistant gene instead of lac Z gene. The competent cells were allowed to take up the plasmid and then plated in the media containing ampicillin, X-gal and IPTG and subjected to blue-white screening. Considering all plasmids were recombinant which one of the following statements correctly describes the outcome of the experiment?

The bacteria which took up the plasmids would grow and give blue colonies.

The bacteria which took up the plasmids would not grow.

The bacteria which took up the plasmids would form white colonies.

All of the bacteria would grow and give white colonies.

https://triyambak.org/courses/csir-net-life-sciences/tpp/pyq?qid=7445&year=2016&month=June

QUESTION ID:2

The sequence of the peptide KGLITRTGLIKR can be unequivocally determined by

Only Edman degradation.

Amino acid analysis and MALDI MS/MS mass spectrometry.

MALDI MS/MS mass spectrometry.

MALDI mass spectrometry after treatment of the peptide with trypsin.

https://triyambak.org/courses/csir-net-life-sciences/tpp/pyq?qid=7446&year=2016&month=June

QUESTION ID:3

From statements on protein structure and interactions detailed below, indicate the correct statement

The concentration of a tryptophan containing protein can be determined by monitoring the fluorescence spectrum of the protein.

A peptide with equal number of Glu and Lys amino acids can show multiple charged species in its clectrospray ionization mass spectrum.

The circular dichroism spectrum of a protein shows predominantly helical conformation. Analysis of its two dimensional NMR spectrum shows predominantly β-structure.

Binding constant can be determined by two interacting molecules by the technique of surface plasmon resonance only if there is strong hydrophobic interactions between them.

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QUESTION ID:4

Radioimmuno assay (RIA) can be employed for the detection of insulin in blood plasma. For this, 125I labelled insulin is mixed and allowed to bind with a known concentration of anti-insulin antibody. A known volume of patients' blood plasma is then added to the conjugate and allowed to compete with the antigen binding sites of antibody. The bound antigen is then separated from unbound ones and the radioactivity of free antigen is then measured by gamma counter. Following are some of the statements made about this assay.

(i) The ratio of radioactive count for unbound antigen to the bound one is more at the end of reaction.

(ii) The ratio of radioactive count for unbound antigen to the bound one is less at the end of reaction.

(iii) For a diabetic patient, the radioactive count for free antigen is less than that for a normal individual.

(iv) For a diabetic patient, the radioactive count for free antigen is more than that for a normal individual.

Which of the above statements are true?

(i) and (iii)

(i) and (iv)

(ii) and (iii)

(ii) and (iv)

https://triyambak.org/courses/csir-net-life-sciences/tpp/pyq?qid=7448&year=2016&month=June

QUESTION ID:5

It is hypothesized that the mean (μ0) dry weight of a female in a Drosophila population is 4.5 mg. In a sample of 16 female with ̅ = 4.8 mg and s = 0.8 mg, what dry weight values would lead to rejection of the null hypothesis at p = 0.05 level? (take t0.05=2.1)

Values lower than 4.0 and values higher than 5.6

Values lower than 3.20 and values higher than 6.40

Values lower than 4.38 and values higher than 5.22

Values lower than 3.22 and values higher than 6.48

https://triyambak.org/courses/csir-net-life-sciences/tpp/pyq?qid=7449&year=2016&month=June

QUESTION ID:6

A researcher wants to obtain complete chemical information, i.e., head groups and fatty acids of phospholipids from liver tissues. Phospholipids have fatty acids of different lengths and unsaturation and also the head groups are of different chemistries. Which of the following combination of techniques would provide complete chemical description of phospholipids?

Only thin layer chromatography (TLC)

TLC and gas chromatography

Paper and thin layer chromatography

Only paper chromatography

https://triyambak.org/courses/csir-net-life-sciences/tpp/pyq?qid=7450&year=2016&month=June