Previous Year Questions

QUESTION ID:2

In circadian rhythm studies, following may be possible generalizations for the effectiveness of light entrainment to the day/ night cycle:

A. Shorter exposures have a greater effect than longer exposures

B. Bright light exposures have a greater effect than dim light

C. Intermittent light exposures have a greater effect than consistent exposures

D. Dim light can affect entrainment relative to darkness

Which combination of the above statements is correct?

QUESTION ID:3

Which one of the following statements regarding 'Endosymbiotic hypothesis of origin of eukaryotes' is INCORRECT?

QUESTION ID:4

To understand the singing behaviour in songbirds, the following three characters were measured as shown in the graph:

A. Territoriality rate

B. Female fertility rat

C. Song rate

Which one of the following conclusions is most appropriate?

QUESTION ID:5

Several fusion constructs were developed to purify heterologous protein in E. coli. The table below lists fusion partners and ligands. Which one of the following is the correct match of the fusion partner with the ligand?

QUESTION ID:6

Given below are four statements regarding genetic transformation of plants in the laboratory:

A. Plants incapable of sexual reproduction cannot be transformed by Agrobacterium tumefaciens

B. Integration oftransgene in organcllar (chloroplast) genome occurs primarily by homologous recombination

C. An enhancer trap construct used in Agrobacterium-mediated transformationn would contain a functional coding sequence of a reporter gene and a minimal promoter

D. A To transgenic plant containing two unlinked copies of a selection marker gene (hpt) and one copy of the passenger gene (gfp) would segregate in a 1:1 ratio for hygromycin resistance: sensitivity in the backcrossed progeny grown on selection media

Which one of the combinations of above statements are correct?

QUESTION ID:7

Agrobacterium mediated transfonnation was used to generate transgenic plants using a construct with a selection marker gene "X“ and a passenger gene "Y". Expression levels of "Y'' protein in eight independent transgenic plants are given below:

The following could represent probable reasons for the observed variability in transgene expression levels

A. Position effects on passenger gene

B. Transgene silencing of the marker gene

C. Variation in copy number of passenger gene

D. mRNA instability of marker gene

 Which one of the following combinations of above statements is correct?

QUESTION ID:8

In a breeding experiment, two homozygous parental lines (PI and P2) were crossed to produce F1 hybrids. Due to an experimental error, seeds of these hybrids got mixed up with the seeds of two other gennplasm lines (P3 and P4) and hybrid seeds derived from them. A marker-based fingerprinting exercise was perfonned using six randomly selected seeds (F 1-F6) from the mixed material and the four parental lines. Results of this analysis are shown below

Based on the above data, which one of the following options represents the correct set of parents and their F 1 progeny?

QUESTION ID:9

The Nuclear Magnetic Resonance ( I D andl 20) spectrum of a 30-residue peptide were: recorded at 25°C. The following observations were made.

A. The NH and caH resonances were welll resolved

B. The NOESY spectra showed extensive: N;- Ni+1 connectivities

C The NH resonances showed slow exchange with deuterium

The spectra indicates that the peptide adopts

QUESTION ID:10

Given below are a set of statistical methods/parameters (Column A) and their potential applications/utility in biological research (column B), in a random manner. Which of the following options is a correct match of entries in Column A and B?

QUESTION ID:11

In an experiment designed to clone a PeRamplified fragment in a cloning vector digested with Xhol (CrrCGAG) and Smal (CCC/GGG), whic:h one of the following combinations of restriction enzymes can be used in the PCR primer to generate compatible ends for cloning? (' /' indicates the site of cleavage within the recognition sequence)

QUESTION ID:12

A researcher was working with three proteins, A, B and C which may have potential roles in gene expression. In order to validate the hypothesis, EMSA (electrophoretic mobility shift assay) was perform The purified proteins were allowed to bind with a labelled DNA and the results obtained after autoradiography are shown below

The following interpretations were made
(i) Protein A possesses the DNA binding motif

(ii) Protein B possesses the DNA binding motif

(iii) Protein 8 binds to DNA ··protein A complex

(iv) Protein C binds to DNA only when protein A is bound

Choose the correct combination of interpret· tations.

QUESTION ID:13

Point group symmetry operations such as inversion and mirror plane are not applicable to protein crystals. This is because