Previous Year Questions

Performance Meter

0%

TLS Online TPP Program

QUESTION ID:1

A neurophysiolgist was interested in using the patch-clamp technique. Following statements are related to this technique:

A. Intracellular movement of ion channels.

B. Post–translational modification of the ion channel protein

C. Ligand that controls the opening or closing of ion channels.

D. Change in current flow in a single ion channel.

Which one of the following combinations will be achievable using the patch-clamp technique?

QUESTION ID:2

The blood plasma proteins(albumin and globulins) from a healthy person were separated by electrophoresis as shown above. The diagnosis of acute inflammation can be done based on one of the following observations.



QUESTION ID:3

Following statements were given regarding factors influencing variation in expression levels of transgene in transgenic plants:

A. Difference in restriction enzyme sites within the T- DNA.

B. Difference in copy number of the transgene.

C. Variations in site of integration of the T- DNA within the plant genome.

D. Presence of multiple promoters within the T-DNA region.

Which one of the following options represents a combinations of statements that would NOT lead to variations in transgene expression levels in transgenic

plants generated using the same T-DNA/ binary vector?

QUESTION ID:4

The above figure shows the fluorescence emission spectra of three different proteins; Protein (X), Protein (Y), and Protein (Z) excited at 280 nm. Which one of the following statements gives the correct interpretation?



QUESTION ID:5

Specimens for light microscopy are commonly fixed with a solution containing chemicals that crosslink/denature cellular constituents. Commonly used fixatives such as formaldehyde and methanol could act in various ways as described below:

A. Formaldehyde crosslinks amino groups on adjacent molecules and stabilizes protein-protein and protein-nucleic acid interactions.

B. Methanol acts as a denaturing fixative and acts by reducing the solubility of protein molecules by disrupting hydrophobic interactions.

C. Formaldehyde crosslinks lipid tails in biological membranes.

D. Methanol acts on nucleic acids. crosslinks nucleic acids with proteins and thus stabilizes protein-nucleic acid interactions.

Which one of the following combinations represents all correct statements?

QUESTION ID:6

Run off transcription assays were performed to establish the specificity of three novel sigma factors for their promoters. Result of the experiments are shown below:


Following inferences were made from these results:

A. σA intiates transcription from P2 and σB from P1

B. σC can intiates transcription from both promoters.

C. σB Prevents intiation of transcription from P2

D. σA Intiates transcription from P1

Choose the option that correctly interprets that results?