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TLS Online TPP Program
QUESTION ID:1
In eukaryotes, microtubules and actin-binding proteins influence the dynamics and organization of the cytoskeleton. Match the cytoskeleton-binding proteins listed in colmm1 B, to actin or microtubule mentioned in Column A, and their function from those listed in Colunm C.
Column A | Column B | Column C |
X. microtubule | (i) katenin | (a) filament cross-linking |
Y. actin | (ii) tropomodulin | (b) severs filaments and binds to plus end |
(iii) tau | (c) prevents assembly and disassembly at minus end | |
(iv) gelsolin | (d) only severs filament |
Which one of the following options represents all the correct matches between columns A, B and C?
QUESTION ID:2
Sucrose-phosphate synthase (SPS) and sucrose-phosphate phosphatase (SPP)
are two l<ey enzyn1es involved in the biosynthesis of sucrose. Following are certain statements regarding these two enzymes:
A. Fructose-6-phosphate is one of the substrates of SPS enzyme.
B. Fn1ctose-6-phosphate and UDP-glucose are the substrates of SPP enzyme.
C. Sucrose is the final product of SPP enzyme.
D. UDP is one of the products of SPS enzyme while Pi is one of the products of SPP enzyme.
Which one of the following options represents the combination of all correct statements?
QUESTION ID:3
The following statements are made regarding the nitrogenase enzyme involved in the reduction of atmospheric nitrogen to ammonia:
A. Nitrogenase is composed of two components: dinitrogenase and dinitrogenase reductase.
B. MoFe protein component is a homodimer.
C. Fe protein component is dinitrogenase.
D. MoFe protein contains the active site metal cluster where N2 binds.
E. Fe protein delivers electron to MoFe protein component in a reaction coupled to the hydrolysis of MgATP.
Which one of the following options represents the combination of all correct statements?
QUESTION ID:4
In four taxa (A, B, C and D), two characters (shape and color) were scored to
infer their phylogenetic relationship. The two character states for shape were square and round, while the two character states for color were black and yellow. The character distribution is given in the table below.
Using the above data, four trees were built using the method of maximum parsimony which are given below
Select the option that represents the two most parsimonious trees
QUESTION ID:5
Given below are a set of statements about metapopulation dynamics and habitat
conservation:
A. The sizes of suitable patches are important because demographic stochasticity can lead to extinction, especially in organisms with low reproductive output.
B. In the incidence function model (IFM), the extinction risk of local
populations increases with increasing habitat patch area, and thecolonization probability is a function of patch isolation from existing local populations.
C. From the conservation perspective, large numbers of suitable patches are not sufficient if distances are too large, preventing recolonization and the rescue etiect.
D. To minimize extinction risk there should be as low a variance in local patch quality as possible, to allow for synchronous dynamics. Which one of the following options represents the combination of all correct statements?
QUESTION ID:6
A researcher isolated chromatin from mammalian cells and digested with tnicrococcalnuclease in different tubes for 1 h, 3 h, 6 h, and 12 h. Thereafter, DNA was purified from all the digested chromatin samples, and two independentSouthern blot hybridization experiments were performed with probe-! and proben. The probe-I and probe-n correspond to different loci of the chromosome(s). The images below represent the Southern blot hybridization pattern generated by probe-I and probe-II.
Following statements were made to explain the results of the Southern blot experiments.
A. Size of probe-II is smaller than probe-I.
B. Probe-I may correspond to the centromeric region of the chromosome.
C. Probe-I may correspond to a hypomethylated locus of the genome.
D. Probe-II may correspond to an euchromatic locus of the genome.
Which one of the following options represents the combination of all correct statements?
QUESTION ID:7
Following statements are about the features of immunoassays used to assay biomolecules:
A Radio-immunoassays (RIAs) are more sensitive than Enzyme-linked immunosorbent assays (ELISAs) with chromogenic substrates.
B. ELISAs with chromogenic substrates are more sensitive than ELISAs with chemiluminogenic substrates.
C. ELI SPOT measures the number of cells capable of secreting particular biomolecules using a substrate that gives soluble product with enzyme reaction.
D. In Western blot analysis, the product of enzyme-substrate reaction local ises at the site precisely where the antibody-enzyme conjugate binds to its specific protein band.Which of the following options represents the combination of all correct statements:
QUESTION ID:8
Different types of mapping populations that can be created using a variety of methods are presented as I to IV in the figure below:
A list of probable mapping populations denoted by I to IV in the figure and their status of genetic mortality is given below.
A. I - Recombinant inbred lines (RILs) - immortal
B. II- Doubled haploid- Not inunortal
C. III - F 2:3 - Not immortal
D. IV- Near isogenic Lines (NILs) - immortal
Which one of the following options represents the combination of all correct matches?
QUESTION ID:9
Members of the chlorophytes are stn1cturally diverse. Select the option that correctly represents the increasing order of structural complexity among the given genera
QUESTION ID:10
India has designated regions as sanctuaries or national parks (column Q) dedicated for the conservation of specific species ( colmm P).
Column P | Column Q | |
A. Gharial | i. | Bhitarkanika Wildlife Sanctuary |
B. Saltwater Crocodile | ii. | National Chambal Sanctuary |
C. Humpback Mahseer | iii. | Gahirmatha Sanctuary |
D. Hawksbill turtle | iv. | Cauvery Wildlife Sanctuary |
Select the option that depicts all correct matches between column P and column
QUESTION ID:11
The following list represents two types of reproductive isolation (Column P) that can lead to speciation. Colunm Q represents the processes by which these isolations can occur.
Column P | Column Q | |
A - Prezygotic | i. | Seasonal |
| B - Postzygotic | ii. | Hybrid inviability |
iii. | F2 breakdown | |
iv. | Stigmatic SI response |
Select the option that represents the correct match between the prezygotic and postzygotic isolation types listed in Colunm P and the processes described in Column Q?
QUESTION ID:12
Bacteriophage λ A and P1 are both temperate phages. ·which one of the following statements made about these phages and their lytic and lysogeny cycles in E. coli is INCORRECT?
QUESTION ID:13
Following statements are made regarding the roles of complement components in immunity:
A. Binding of complement components to antigen presenting cells decreases their phagocytic ability and modulates cytokine secretion.
B. Complement components enhance the B cell-mediated immune response
by increasing the avidity with which a B cell binds to a complement-bound antigen.
C. Immature T cells are protected from the natural antibody and complementmediated lysis by provision of additional sialic acid residues on their cell surface glycoproteins.
D. Binding of C3a, C5a and C3b to their respective receptors on mature T cells inhibits their growth, di±Ierentiation, and survival.
E. During the contraction phase of the immune response, excess lymphocytes that were made during antigen-induced expansion are eliminated by apoptosis, with the help ofClq complement component.
Which of the following options represents the combination of all correct statements:
QUESTION ID:14
Which one of the following statements is correct in the context of erythromycinmediated inhibition of protein synthesis in bacteria?
QUESTION ID:15
Red-blue colour blindness is a human X-linked recessive disorder. The two parents with normal colour vision have two sons. Son 1 has 47, XXY
chromosome composition and is colour blind. Son 2 has 46, XY and is also colour blind. Assuming that no crossing over took place in prophase I of meiosis, Klinefelter syndrome in Son 1 resulted due to nondisjunction during which one of the following events?
QUESTION ID:16
The following statements are made about digestion of proteins and the enzymes involved:
A. Chymotrypsin does not generate peptides with C-terminal neutral amino acids.
B. Trypsin generates peptides with C-terminal basic amino acids.
C. Carboxypeptidase B acts on aromatic amino acids.
D. Carboxypeptidase A employs zinc ion for hydrolysis.
E. The brush border enterokinase has no polysaccharides attached to it.
F. The final digestion to amino acids occurs in the intestinal lumen, the brush border, and the cytoplasm of the mucosal cells. Which one of the following options represents the combination of all correct statements?
QUESTION ID:17
ERK5 is a MAP kinase that is activated upon phosphorylation by MEK5.
MEK5 binds with MEKK2 when co-expressed. HEK293 cells were transfected with plasmid encoding ERK5, along with plasmids encoding either MEK5 alone, or MEKK2 alone, or both MEKK2 and MEK5, or both MEKK2 and MEK5AA (MEK5 mutant). Lysates oftransfected cells were analysed by Western blotting using anti-ERK5 antibody as shown below:
From the data in the figure above, the following conclusions were drawn:
A. Full activation of ERK5 requires both MEKK2 and MEK5.
B. Phosphorylation with MEKK2 alone suggests that it can activate ERK5 without MEK5.
C. Difference in the levels of phosphorylation with MEKK2 alone and
MEKK2 + MEK5 is due to more phosphorylation at the same site.
D. Phosphorylation with only MEKK2 transfection suggests that it might be associating with endogenous MEK5 to get partially activated, leading to ERK5 phosphorylation to some extent.
E. MEK5AA might be a dmninant-negative mutant ofMEK5 which preventssignalling through active, endogenous MEK5.
Which of the following options represents the combination of all correct statements?
QUESTION ID:18
Alleles A, a, B and b can be distinguished on the basis of their mobility on an agarose gel. These genes are present on the same chromosome. In the gel image below, the band patten1 reflects the alleles in parents and their progeny (number reflects the progeny counted).
Which one of the following statements correctly explains the band pattern?
QUESTION ID:19
The following are certain statements regarding the PSII electron carrier during the light reaction of photosynthesis:
A. The first electron released frmn reaction centre P680 is transferred to QA to produce a plastosemiquinone.
B. QA is the 1nobile plastoquinone.
C. The first electron transferred from QA to QB converts QB into plastosen1- iqinone
D. QB is tightly bound to the complex and is not 1nobile. Which one of the following options represents the correct statement(s)?
Which one of the following options represents the correct statement(s)?
QUESTION ID:20
In many sexually reproducing organisms, females make mate choice decisions based on male display traits. Several models have been proposed to explain the evolution of exaggeration in male traits. Two of them have been given below in column P and their possible descriptions in column Q.
Column P | Column Q | |
i. | Males exploit a pre-existing sensory bias in females. Females choosing such males have higher quality offspring. | |
A. Runaway Selection | ii. | Female choice for male trait occurs due to perceptual errors leading to poor quality offspring. Thus, females will evolve to run away from males with such traits. |
| B. Chase-away selection | iii. | Female choice for male trait results in a positive feedback loop favouring both, males with such trait and females that prefer them. |
iv. | Males exploit pre-existing sensory bias in females. Females do not benefit by choosing such males, driving the evolution of females that discriminate against such males. |
Match the models to their appropriate description and choose the correct option.
QUESTION ID:21
Given below are a few terms related to Bioinfom1atics resources (Colunm X) and their functions/applications (Column Y):
Column X | Column Y | |
Resources | Functions /applications | |
A. TrEMBL | i. | Analysis of recombination frequencies between molecular markers |
B. TBLASTN | ii. | Database of protein sequences |
C. SCOP | iii. | Comparison of amino acid sequence against nucleotide sequence database translated in all six reading frames |
D. JoinMap | iv. | Manually curated structural classification of proteins |
Which one of the following options represents all correct matches between Column X and Column 1 Y?
QUESTION ID:22
A scientist is using the Hardy-Weinberg equation to assess if a population is in equilibrium or is evolving. She recorded the following characteristics for this population:
A.The size of the population is very large.
B. Individuals are randomly mating.
C. Individuals are under natural selection.
D. New alleles are added to the population through tnigration and dispersal.
E. Mutation rates are high. Which one of the following options contains all INCORRECT characteristics of a population in Hardy-Weinberg equilibrium?
QUESTION ID:23
The following patten1s of gene expression were obtained after activating Notch I receptor with two different ligands Dill , and DII4.
Which one of the following statements depicts the correct interpretation of observations?
QUESTION ID:24
The following statements suggest the changes in respiratory ventilation and the mechanisms of these changes when a normal human subject is allowed to inhale air containing different oxygen content:
A. The ventilation is markedly increased when Poz of the inspired air is less than 60 mm Hg.
B. The ventilation is 6 L/min when the Po2 of the inspired air is about 150 n1mHg.
C. The ventilation is slightly increased when Po2 of the inspired air is more than 60 mm Hg.
D. The increased ventilation due to the lower Po2 in the inspired air causes higher alveolar Pcoz.
E. The H+ concentration in the arterial blood is increased when Po2 of the inspired air is gradually decreased.
Which one of the following options represents the combination of all correct statements?
QUESTION ID:25
It has been observed that within a flowering season, a plant may produce more male flowers which may be correlated with the longevity of the flowers and the seasonal distribution of t1owering in the plant. Which one of the following argutnents do NOT support this observation of sex specific floral phenology.
QUESTION ID:26
The following statements are made about the E. coli SOS response to DNA damage:
A. RecA-DNA filament complex stimulates the autoproteolytic activity of the LexA repressor.
B. RecA is activated due to the blunt ends of double-strand breaks caused by DNA damage-inducing agents.
C. The SOS response includes the activation of synthesis oftranslesion polymerases.
D. The destruction of LexA promotes synthesis of photo lyase, which acts along with RecA to reverse the pyrimidine dimer formation process.
Which one of the following options represents the combination of all correct statements?
QUESTION ID:27
The following trees represent different evolutionary mechanisms
Select the tree that best represents punctuated equilibrim
QUESTION ID:28
Four different plant communities that consisted of the same number of species were taken up for a species diversity study. The following table represents some of the outcomes:
Community | Simpson's Reciprocal Index Value |
A | 7.25 |
B | 8.20 |
C | 6.80 |
D | 7.05 |
Select the correct statement about the evem1ess of the above communities.
QUESTION ID:29
The following statements refer to factors regulating the fidelity of DNA replication.
A. The 5' to 3' exonuclease activity of the replicative DNA polymerase.
B. hnbalanced intracellular concentrations of the four dNTPs.
C. Increased intracellular concentrations of rNTPs resulting in increased
incorporation of rNTPs during DNA synthesis, which are not easily removed by the polymerase 's proof-reading activity.
D. Removal of incorrectly incorporated nucleotides by the mismatch repair system.
Which one of the following options gives the combination of all correct statements?
QUESTION ID:30
A transgenic plant having a homozygous single-copy insertion for trait A was retransfonned by Agrobacterium-mediated transformation with a gene conferring trait B. Given below are a few statements regarding the above experiment:
A. All To transgenic plants obtained after re-transfonnation would be single copy events for both traits, A and B.
B. Tl progeny generated by self-pollination of single-copy transgenic plants obtained by retransformation would segregate in a 3: 1 ratio for trait A.
C. Plant selection marker genes used for transformation experiments for both traits, A and B should be necessarily identical. Different selection marker genes cannot be used.
D. 25% of T1 progeny generated by self-pollination of single-copy transgenic plants obtained by retransfonnation would be homozygous for both traits, A and B.
Which one of the following options represents all INCORRECT statements?
QUESTION ID:31
In the context of signaling, the enzyme protein kinase C (PKC) depends on multiple molecules for its complete activation. This activation depends on the presence of:
A. phosphatidylserine at the inner leaflet of the plasma membrane. B. Ca2+
C. phosphatidylethanolamine at the inner leaflet of the plasma n1embrane.
D. diacylglycerol present in the inner leat1et of the plasma membrane
Which one of the following options represents the combination of all correct molecules?
QUESTION ID:32
Which one of the following statements pertaining to global ocean ecosystem productivity is NOT correct?
QUESTION ID:33
The diagram below depicts the cumulative fossil C02 emissions (y axis) of different continents from the years 1850-2020 (x axis).
Select the option that correctly identifies the continents A-D:
QUESTION ID:34
Ecologists examined the role of competition for below ground resources (water and nutrients) in the dispersion pattern of trees in the Acacia savannas of South Africa. The figure below depicts the result of their study.
In case all the other parameters were constant, select the option that best represents the dispersion patterns for populations labelled A and B in the figure above
QUESTION ID:35
Which one of the following options correctly lists ecosystems of the world arranged according to the descending order of their average world net primary production (billion kcal/yr)?
QUESTION ID:36
Aspartate (Asp) is an amino acid with the structure NH2-CH(CH.z-COOH)COOH. Given below are biosynthetic processes occurring in cells:
A. protein synthesis
B. de novo synthesis of inosine monophosphate and orotic acid
C. synthesis of adenosine monophosphate from inosine monophosphate
D. glutathione synthesis
Which one of the following options correctly represents all the biosynthetic process( es) wherein Asp is involved as a precursor?
QUESTION ID:37
Based on the theory of kin selection, choose the correct statement:
QUESTION ID:38
The following statements were made about the structure of the 30-nm chromatin fiber:
A. In the solenoid model, the linker DNA com1ects the consecutive core particles.
B. In the zig-zag model, alten1ating nucleosomes become interacting neighbors.
C. In the solenoid model, 12 nucleosomes are organized into two separate stacks, whereas 8 nucleosomes per tum make a single stack in the zig-zag model.
D. H l histone is essentially required as per the zig-zag model, but not as per the solenoid model.
E. Chromatin fibers prepared with H4 histones that lack their tails could fold into higher-order fibers.
Which one of the fo llowing options represents the combination of all correct statements?
QUESTION ID:39
A polypeptide was subjected to the following treatments with the indicated results.
I. Acid hydrolysis:
(1) (Ala, Arg, Cys, Glx, Gly, Lys, Leu, Met, Phe, Thr)
II. Aminopeptidase M:
(2) No fragments.
III. Carboxypeptidase A + Carboxypeptidase B:
(3) No fragments.
IV Trypsin followed by Edman degradation of the separated products:
( 4) Cys-Gly-Leu-Phe-Arg
(5) Thr-Ala-Met-Gln-Lys
Which one of the following represents the primary stn1cture of the peptide?
QUESTION ID:40
Genetic screens for mutants affecting development of leaf trichomes have led to the discovery of genes regulating trichome patterning - especially trichome density and spacing, as depicted in the figure below.
The following statements are made in this regard:
A. GLABRAJ ( GLJ) mutant plant will show fewer or no trichomes.
B. Cells that form trichomes strongly express the GLABRA2 ( GL2) and TRYPTICOJV (TRY) genes.
C. TRY protein acts as a positive regulator of trichon1e cell differentiation in the surrounding cells.
D. Addition of exogenous JA will reduce the number ofleaftrichomes
Which one of the following options represents the combination of all correct statements?
QUESTION ID:41
Consumption of untreated corn as the staple food causes the disease, pellagra. Pre-treatment of corn with Ca(OH)2 prevents this disease. Given below are options listing possible effects of Ca(OH)2 treatment (Column X) and the enzymes affected (Column Y).
Column X | Column Y | |
Possible effects of Ca(OH)2 treatment | Enzyme/s affected | |
A. Release of Vitamin B3 upon Ca(OH)2 treatment | i | Activity of trypsin |
B. Alkaline pH aids in digestion. | ii | Enzymes of the TCA cycle |
C. Ca improves bone strength | iii | Activity of NAD-dependent dehydrogenases |
D. Prevents formation of ROS | iv | Activity of cathepsin K |
Select the correct match relevant for preventing pellagra from the options listed below.
QUESTION ID:42
The following statements are made below about hearing phenomena of sound waves:
A. The loudness of a sow1d is inversely correlated with the amplitude of a sound wave.
B. The loudness of a sound is directly correlated with the amplitude of a sound wave.
C. The pitch of a sound is directly correlated with the frequency of the sound wave.
D . The pitch of a sound is inversely correlated with the frequency of the sound wave.
E. The pitch of the average 1nale voice in conversation is lower than that ofthe average female voice.
F. The pitch of the average 1nale voice in conversation is higher than that of the average female voice.
Choose the combination of all correct statements:
QUESTION ID:43
100 
of cells were taken in a tube and 400 0.4% Trypan Blue was added for
of cells were taken in a tube and 400 0.4% Trypan Blue was added forstaining. About 20 of this cell suspension was added between the hemocytometer and cover glass (refer figure below). The hemocytometer is divided into 9 major squares of 1 mm x 1 mm size. The height of the chamber formed with the cover glass is 0.1 mm. Empty circles indicate unstained cells and solid circles indicate stained cells.
of this cell suspension was added between the hemocytometer and cover glass (refer figure below). The hemocytometer is divided into 9 major squares of 1 mm x 1 mm size. The height of the chamber formed with the cover glass is 0.1 mm. Empty circles indicate unstained cells and solid circles indicate stained cells.
Based on the above figure, what is the total cell count in the original suspension and cell viability (% )?
QUESTION ID:44
Mass spectrum of a pure peptide recorded in the positive ion mode is shown below.
(A) What is the reason for multiple peaks in the mass spectrun1 of a pure peptide? (B) Which peak corresponds to the monoisotopic species of the peptide? (C) What is the mono isotopic mass of the peptide? Select the right answers from the options given below.
QUESTION ID:45
Amborellaceae, Aristolochiaceae, Illiciaceae and Winteraceae are four angiosperm families that, according to the APG IV system of classification belong to the 'early diverging angiosperms ' . The presence (V+) or absence (V-) of vessels
in the xylem and the fusion of the carpels within the gynoecimn are important angiospem1 characters. 'A' and ' S' indicate apocarpous (or n1onocarpellary) and
syncarpous condition of ovaty, respectively. Which one of the following options correctly represents the characters found in the above families ?
QUESTION ID:46
Given below are terms related to various techniques (Column X) and their features (Colunm Y):
| Column X (Techniques) | Column Y (Features) |
|---|---|
| A. Reverse transcription | iv. cDNA library |
| B. Southwestern blotting | i. Detection of clones encoding nucleic acid binding proteins |
| C. Genome walking PCR | ii. Determination of 5’ and 3’ DNA sequences flanking a known gene |
| D. RACE | iii. Cloning of cDNA ends |
Which one of the following options represents all correct matches between Colunm X and Column Y?
QUESTION ID:47
Some properties of enzymes are listed in column X, and their kinetic expressions are listed in column Y.
Column X | Column Y | |
Enzyme properties Expressions | Expressions | |
A. Specific activity | i. | kcat/Km |
B. Turnover number | ii. | substrate concentration at which |
C. Michaelis constant | iii. | Vmax/moles of enzyme |
D. Catalytic efficiency | iv. | Vmax/protein concentration |
Which one of the following options represents all correct matches betweenColunm X and Colmru1 Y?
QUESTION ID:48
A food chain involving Spartina (a plant), the marsh periwinkle snail, the blue crab and an unknown fungus was identified in a Spartina-dominated salt marsh
in North America. A study involving control and crab-exclusion experiments revealed: A. Radulations (scrape marks) on the leaf surface made by the snails indicate the presence of snail faeces, fungi and dead plant tissue.
B. The fungi were present only at the radulations.
C. The density of the radulations increased with higher snail densities.
D. Spartina density decreased with increase in the snail density till it reached zero.
E. In control experiments, all four species were present till the end.
Select the option that correctly depicts the positive ( +) and negative (-) interaction-type between fungi-snail and Spartina-crab, respectively: