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TLS Online TPP Program
QUESTION ID:1
The following statements were made about the structure of the 30-nm chromatin fiber:
A. In the solenoid model, the linker DNA com1ects the consecutive core particles.
B. In the zig-zag model, alten1ating nucleosomes become interacting neighbors.
C. In the solenoid model, 12 nucleosomes are organized into two separate stacks, whereas 8 nucleosomes per tum make a single stack in the zig-zag model.
D. H l histone is essentially required as per the zig-zag model, but not as per the solenoid model.
E. Chromatin fibers prepared with H4 histones that lack their tails could fold into higher-order fibers.
Which one of the fo llowing options represents the combination of all correct statements?
QUESTION ID:2
A polypeptide was subjected to the following treatments with the indicated results.
I. Acid hydrolysis:
(1) (Ala, Arg, Cys, Glx, Gly, Lys, Leu, Met, Phe, Thr)
II. Aminopeptidase M:
(2) No fragments.
III. Carboxypeptidase A + Carboxypeptidase B:
(3) No fragments.
IV Trypsin followed by Edman degradation of the separated products:
( 4) Cys-Gly-Leu-Phe-Arg
(5) Thr-Ala-Met-Gln-Lys
Which one of the following represents the primary stn1cture of the peptide?
QUESTION ID:3
Genetic screens for mutants affecting development of leaf trichomes have led to the discovery of genes regulating trichome patterning - especially trichome density and spacing, as depicted in the figure below.
The following statements are made in this regard:
A. GLABRAJ ( GLJ) mutant plant will show fewer or no trichomes.
B. Cells that form trichomes strongly express the GLABRA2 ( GL2) and TRYPTICOJV (TRY) genes.
C. TRY protein acts as a positive regulator of trichon1e cell differentiation in the surrounding cells.
D. Addition of exogenous JA will reduce the number ofleaftrichomes
Which one of the following options represents the combination of all correct statements?
QUESTION ID:4
Consumption of untreated corn as the staple food causes the disease, pellagra. Pre-treatment of corn with Ca(OH)2 prevents this disease. Given below are options listing possible effects of Ca(OH)2 treatment (Column X) and the enzymes affected (Column Y).
Column X | Column Y | |
Possible effects of Ca(OH)2 treatment | Enzyme/s affected | |
A. Release of Vitamin B3 upon Ca(OH)2 treatment | i | Activity of trypsin |
B. Alkaline pH aids in digestion. | ii | Enzymes of the TCA cycle |
C. Ca improves bone strength | iii | Activity of NAD-dependent dehydrogenases |
D. Prevents formation of ROS | iv | Activity of cathepsin K |
Select the correct match relevant for preventing pellagra from the options listed below.
QUESTION ID:5
The following statements are made below about hearing phenomena of sound waves:
A. The loudness of a sow1d is inversely correlated with the amplitude of a sound wave.
B. The loudness of a sound is directly correlated with the amplitude of a sound wave.
C. The pitch of a sound is directly correlated with the frequency of the sound wave.
D . The pitch of a sound is inversely correlated with the frequency of the sound wave.
E. The pitch of the average 1nale voice in conversation is lower than that ofthe average female voice.
F. The pitch of the average 1nale voice in conversation is higher than that of the average female voice.
Choose the combination of all correct statements:
QUESTION ID:6
100 
of cells were taken in a tube and 400 0.4% Trypan Blue was added for
of cells were taken in a tube and 400 0.4% Trypan Blue was added forstaining. About 20 of this cell suspension was added between the hemocytometer and cover glass (refer figure below). The hemocytometer is divided into 9 major squares of 1 mm x 1 mm size. The height of the chamber formed with the cover glass is 0.1 mm. Empty circles indicate unstained cells and solid circles indicate stained cells.
of this cell suspension was added between the hemocytometer and cover glass (refer figure below). The hemocytometer is divided into 9 major squares of 1 mm x 1 mm size. The height of the chamber formed with the cover glass is 0.1 mm. Empty circles indicate unstained cells and solid circles indicate stained cells.
Based on the above figure, what is the total cell count in the original suspension and cell viability (% )?
QUESTION ID:7
Mass spectrum of a pure peptide recorded in the positive ion mode is shown below.
(A) What is the reason for multiple peaks in the mass spectrun1 of a pure peptide? (B) Which peak corresponds to the monoisotopic species of the peptide? (C) What is the mono isotopic mass of the peptide? Select the right answers from the options given below.
QUESTION ID:8
Amborellaceae, Aristolochiaceae, Illiciaceae and Winteraceae are four angiosperm families that, according to the APG IV system of classification belong to the 'early diverging angiosperms ' . The presence (V+) or absence (V-) of vessels
in the xylem and the fusion of the carpels within the gynoecimn are important angiospem1 characters. 'A' and ' S' indicate apocarpous (or n1onocarpellary) and
syncarpous condition of ovaty, respectively. Which one of the following options correctly represents the characters found in the above families ?
QUESTION ID:9
Given below are terms related to various techniques (Column X) and their features (Colunm Y):
| Column X (Techniques) | Column Y (Features) |
|---|---|
| A. Reverse transcription | iv. cDNA library |
| B. Southwestern blotting | i. Detection of clones encoding nucleic acid binding proteins |
| C. Genome walking PCR | ii. Determination of 5’ and 3’ DNA sequences flanking a known gene |
| D. RACE | iii. Cloning of cDNA ends |
Which one of the following options represents all correct matches between Colunm X and Column Y?
QUESTION ID:10
Some properties of enzymes are listed in column X, and their kinetic expressions are listed in column Y.
Column X | Column Y | |
Enzyme properties Expressions | Expressions | |
A. Specific activity | i. | kcat/Km |
B. Turnover number | ii. | substrate concentration at which |
C. Michaelis constant | iii. | Vmax/moles of enzyme |
D. Catalytic efficiency | iv. | Vmax/protein concentration |
Which one of the following options represents all correct matches betweenColunm X and Colmru1 Y?
QUESTION ID:11
A food chain involving Spartina (a plant), the marsh periwinkle snail, the blue crab and an unknown fungus was identified in a Spartina-dominated salt marsh
in North America. A study involving control and crab-exclusion experiments revealed: A. Radulations (scrape marks) on the leaf surface made by the snails indicate the presence of snail faeces, fungi and dead plant tissue.
B. The fungi were present only at the radulations.
C. The density of the radulations increased with higher snail densities.
D. Spartina density decreased with increase in the snail density till it reached zero.
E. In control experiments, all four species were present till the end.
Select the option that correctly depicts the positive ( +) and negative (-) interaction-type between fungi-snail and Spartina-crab, respectively: