Previous Year Questions

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QUESTION ID:1

Mass spectrum of a pure peptide recorded in the positive ion mode is shown below.
(A) What is the reason for multiple peaks in the mass spectrun1 of a pure peptide? (B) Which peak corresponds to the monoisotopic species of the peptide? (C) What is the mono isotopic mass of the peptide? Select the right answers from the options given below.

QUESTION ID:2

Amborellaceae, Aristolochiaceae, Illiciaceae and Winteraceae are four angiosperm families that, according to the APG IV system of classification belong to the 'early diverging angiosperms ' . The presence (V+) or absence (V-) of vessels
in the xylem and the fusion of the carpels within the gynoecimn are important angiospem1 characters. 'A' and ' S' indicate apocarpous (or n1onocarpellary) and
syncarpous condition of ovaty, respectively. Which one of the following options correctly represents the characters found in the above families ?

QUESTION ID:3

Given below are terms related to various  techniques (Column X) and their features (Colunm Y):
Column X (Techniques) Column Y (Features)
A. Reverse transcription iv. cDNA library
B. Southwestern blotting                                            i. Detection of clones encoding nucleic acid binding proteins
C. Genome walking PCR ii. Determination of 5’ and 3’ DNA sequences flanking a known gene
D. RACE iii. Cloning of cDNA ends
Which one of the following options represents all correct matches between Colunm X and Column Y?

QUESTION ID:4

Some properties of enzymes are listed in column X, and their kinetic expressions are listed in column  Y.

       Column X

                         Column Y

Enzyme properties Expressions

                      Expressions

A. Specific activity

i.

kcat/Km

B. Turnover number

ii.

substrate concentration at which

C. Michaelis constant

iii.

Vmax/moles of enzyme

D. Catalytic efficiency

iv.

Vmax/protein concentration

Which one of the following options represents all correct matches betweenColunm X and Colmru1 Y?

QUESTION ID:5

A food chain involving Spartina (a plant), the marsh periwinkle snail, the blue crab and an unknown fungus was identified in a Spartina-dominated salt marsh
in North America. A study involving control and crab-exclusion experiments revealed: A. Radulations (scrape marks) on the leaf surface made by the snails indicate the presence of snail faeces, fungi and dead plant tissue.
B. The fungi were present only at the radulations.
C. The density of the radulations increased with higher snail densities.
D. Spartina density decreased with increase in the snail density till it reached zero.
E. In control experiments, all four species were present till the end.
Select the option that correctly depicts the positive ( +) and negative (-) interaction-type between fungi-snail and Spartina-crab, respectively: