Previous Year Questions

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QUESTION ID:1

Which of the following sugar derivative(s) is found in bacterial cell wall?

(A). Lipopolysaccharide (LPS)

(B). Lipid A

(C). N-acetyl muramic acid

(D). D-galactosamine

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QUESTION ID:2

Name the phase of microbial growth cycle when there is no net increase or decrease in cell number.

QUESTION ID:3

The organisms which have the ability to grow in a very dry environment are known as:

QUESTION ID:4

Match List-I with List-II

List-IList-II
(A). Chemostat(I). The culture is maintained at high cell density by repeated addition of the nutrient till the reactor reaches its maximum capacity
(B). Turbidostat(II). It is a closed culture system
(C). Batch culture(III). Growth rate and cell density of the culture is controlled by the concentration of limiting nutrient
(D). Fed Batch Culture(IV). Growth rate and cell density of the culture is controlled by the turbidity of the culture
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QUESTION ID:5

Calculate the melting temperature (°C) of the following primer:

Primer Sequence  5'-AGCTAATCCGGGCTACCG-3'

QUESTION ID:6

Arrange the following steps of polymerase chain reaction in correct sequence.

(A). Annealing
(B). Denaturation
(C). Cooling at 4 °C
(D). Extension

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QUESTION ID:7

In a vector, antibiotic resistance is often used as a:

QUESTION ID:8

Which of the following is the most abundant protein on earth?

QUESTION ID:9

Match List-I with List-II

List-I (Microorganism)List-II (Category)
(A). Azotobacter(I). Free living nitrogen fixing phototrophic bacteria
(B). Rhodobacter(II). Free living nitrogen fixing chemoorganotrophic bacteria
(C). Rhizobium(III). Free living nitrogen fixing chemolithotrophic bacteria
(D). Alcaligenes(IV). Symbiotic nitrogen fixing bacteria associated with peas
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QUESTION ID:10

Which of the following biological materials has not been patented?

QUESTION ID:11

In vertical gel electrophoresis, the function of the β-mercaptoethanol is:

QUESTION ID:12

Match List-I with List-II

List-I (Author)List-II (Contribution)
(A). Martinus Beijerinck(I). One gene-one enzyme hypothesis
(B). Sergei Winogradsky(II). First time isolation of nitrogen fixing bacterium Clostridium pasteurianum
(C). George Beadle and Edward Tatum(III). Restriction enzymes
(D). Hamilton Smith, Daniel Nathans, Werner Arber(IV). Enrichment culture technique
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QUESTION ID:13

Arrange the steps followed in the differential Gram staining of bacteria.

(A). Add iodine solution for 1 minute

(B). Stain the smear with crystal violet for 1 minute

(C). Spread culture in thin film and air dry followed by heat fixing

(D). Counter stain the smear with safranin solution for 1-2 minutes

(E). Decolorize with alcohol briefly for 20 seconds

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QUESTION ID:14

Coenzymes and Prosthetic groups are small non-protein molecules that take part in catalysis. What is the major difference between the two cofactors?

QUESTION ID:15

How many ATPs are produced on complete oxidation of 1 glucose molecule through oxidative phosphorylation and substrate-level phosphorylation?

QUESTION ID:16

Flow of genetic information from DNA to RNA is known as transcription. Which of the following represents RNA transcript sequence for the given DNA molecule?

5'-TTAGCCTCGTGTAA-3'

3'-AATCGGAGCACATT-5'

QUESTION ID:17

Match List-I with List-II

List-I (Enzyme)List-II (Function)
(A). DNA Pol I(I). Relaxes supercoils ahead of replication fork
(B). DNA gyrase(II). Unwinds DNA double helix at replication fork
(C). DNA ligase(III). Excises RNA primer and fills in gaps
(D). Helicase(IV). Seals nicks in DNA
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QUESTION ID:18

What is a missense mutation?

QUESTION ID:19

Which of the following facts is/are true about Shuttle vectors?

(A). Vectors that have the capability to replicate in only related host organisms

(B). Vectors that have the capability to replicate in two unrelated host organisms

(C). Shuttle vectors have two origins of replication

(D). Shuttle vectors do not have a selection marker

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QUESTION ID:20

To purify RNA molecules, oligo dT containing matrix is used. This type of chromatography is:

QUESTION ID:21

Which of the following can not be separated using electrophoresis?

QUESTION ID:22

Arrange the sequence of regulation of chemotaxis.

(A) Interaction with flagellar motor switch

(B) Adaptation

(C) Activation of response regulators

(D) Interaction of transducers and Che proteins

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QUESTION ID:23

Arrange the replication cycle of a bacterial virus.

(A) Synthesis of nucleic acid and protein by host cell

(B) Penetration of virion

(C) Attachment of the virion to the host

(D) Assembly of capsid and packaging of genome into new virion

(E) Release of mature virions

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QUESTION ID:24

During cell cycle, DNA duplication and transcription occur during:

QUESTION ID:25

Which of the following statements about Denitrification are true?

(A). Atmospheric nitrogen is easily used as a source of nitrogen by bacteria than nitrate.

(B). Denitrification is conversion of nitrate to gaseous nitrogen compounds.

(C). Denitrification is a detrimental process for agriculture.

(D). Denitrification is a beneficial process in waste water treatment.

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QUESTION ID:26

Mycorrhizae is an association between:

QUESTION ID:27

Which of the following oligonucleotide probes (fluorescent tag) will hybridize to a target RNA sequence?

5'-GAAACCUCUGGCAAUCCGAUGUGCCAA-3'

QUESTION ID:28

Which of the following is not used as mobile phase in gas chromatography?

QUESTION ID:29

Arrange the following vectors on the basis of insert size carrying capacity.

(A). Cosmids

(B). Bacteriophages

(C). Plasmids

(D). Bacterial Artificial Chromosomes

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QUESTION ID:30

Which of the following technique is known as chain termination DNA sequencing method?

QUESTION ID:31

Which of the following is not a fermented food product?

(A). Sauerkraut

(B). Sake

(C). Soy Sauce

(D). Ragi

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QUESTION ID:32

Solutes in the environment change the availability of water molecules to microbes. Microbiologists quantitatively estimate the degree of water availability by determining:

QUESTION ID:33

When a petite mutant isolated from aerobically cultured yeast is crossed with wild-type strain, the progeny obtained is all wild type. Identify the type of mutation.

QUESTION ID:34

Which of the following is not a characteristic feature of actinomycetes?

QUESTION ID:35

Which of the following are features of chlorosomes?

(A). Light harvesting

(B). Contain chlorophyll a

(C). Present in green bacteria

(D). Galactolipid membrane

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QUESTION ID:36

What are the functions of peroxisomes in the cell?

(A). Peroxisomes are the sites where oxidation of substrates takes place and hydrogen peroxide is formed.

(B). Peroxisomes harbor catalase enzyme which decomposes hydrogen peroxide.

(C). In plants and fungi, fatty acid oxidation takes place in the peroxisomes.

(D). In animal cells, fatty acid oxidation takes place in the peroxisomes and mitochondria.

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QUESTION ID:37

If organism A possesses 55% G+C content and organism B has 25% of the G+C content in their genome, what inference can be drawn?

QUESTION ID:38

Out of all given options, which ones are stop codons?

QUESTION ID:39

Which of the following is not an Antigen Presenting Cell?

QUESTION ID:40

Match List-I with List-II 

List-I (Amino acids)List-II (Side chain type)
(A). Cys(I). basic
(B). Asp(II). nonpolar aromatic
(C). Trp(III). acidic
(D). Arg(IV). sulphur containing
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QUESTION ID:41

Arrange the following steps of complement activation in the correct order.

(A). Formation of C3 convertase

(B). Binding of C1 to Antigen antibody complex

(C). Formation of MAC

(D). C5 convertase formation

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QUESTION ID:42

Enzyme sequence involved in formation of L-isoleucine from L-Threonine is regulated by:

QUESTION ID:43

Which of the following statements are true about human ABO blood groups?

(A). Glycosyltransferase enzyme is responsible for transfer of carbohydrate moiety on protein.

(B). Almost all individuals possess H substance.

(C). A antigen has one extra monosaccharide group N-acetylgalactosamine added to H substance.

(D). B antigen has one extra monosaccharide group galactose added to H substance.

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QUESTION ID:44

Which of the following is not used as a cyanobacterial biofertilizer?

QUESTION ID:45

Which of the following cause depletion of ozone layer?

QUESTION ID:46

Arrange the steps of binary fission in a rod shaped prokaryote in sequence.

(A). Cell elongation

(B). Cell separation

(C). Septum formation

(D). DNA replication

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QUESTION ID:47

TCR of Cytotoxic T lymphocytes interacts with which of the following?

QUESTION ID:48

The first cells to reach the site of inflammation are:

QUESTION ID:49

Arrange the steps for wastewater treatment processes in correct sequence.

(A). Screening and Sedimentation

(B). Anoxic digestion and oxidation

(C). Separation into soluble and insoluble components

(D). Drying and Disinfection of the two components

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QUESTION ID:50

What is the major activity occurring in germinal centers?

QUESTION ID:51

Match List-I with List-II

List-I (Interaction type)List-II (Effect)
(A). Commensalism(I). Where one population derives food from the host without causing death of the host
(B). Competition(II). Population one is inhibited and other is not affected
(C). Amensalism(III). One population benefits and other population (host) is not affected
(D). Parasitism(IV). Each species is directly inhibited by the other
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QUESTION ID:52

Which of the following is an exotoxin producing anaerobic organism associated with food poisoning?

QUESTION ID:53

Which of the following is not a mechanism of antimicrobial resistance?

QUESTION ID:54

Arrange the following intermediates on the basis of their synthesis in citric acid cycle.

(A). Citrate
(B). Succinyl CoA
(C). α-ketoglutarate
(D). Oxaloacetate

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QUESTION ID:55

Which of the following statements is/ are true about agglutination?

(A). Excess antibody inhibits agglutination reactions.

(B). Coombs test works on the principle of agglutination.

(C). Agglutination reactions work only with soluble antigens.

(D). The antibody titer is the lowest dilution of serum at which agglutination is observed.

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QUESTION ID:56

Arrange the steps in RNA synthesis in the correct order.

(A). Release of polymerase

(B). Release of sigma factor followed by elongation

(C). Recognition of promoter by sigma factor

(D). Identification of terminator site

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QUESTION ID:57

What is the function of impellers in a fermentor?

QUESTION ID:58

Which of the following enzymes is responsible for synthesis of mRNA in prokaryotes? (DROP)

QUESTION ID:59

Which of the following is an example of DNA containing virus?

QUESTION ID:60

Which of the following tests are used to check spoilage of milk?

(A). Clot on boiling test

(B). Resazurin reduction assay

(C). MBRT

(D). MPN test

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QUESTION ID:61

Which of the following is not a feature of peptide bond?

QUESTION ID:62

Which of the following media is used for isolation of Staphylococcus?

QUESTION ID:63

Which of the following is exclusively recessive X-linked trait ?

QUESTION ID:64

In a dihybrid cross, if the percentage of recombinant trait is 20%, what will be the distance between the genes?

QUESTION ID:65

Which of the following is not a primary metabolite?

QUESTION ID:66

What is the mode of action of alcohol as a sanitizer and disinfectant?

QUESTION ID:67

Which of the following is/are distinguishing features of group translocation?

(A). A molecule is chemically modified when transported into the cell.

(B). Molecule doesn't get modified during transport.

(C). Molecule gets phosphorylated during transport.

(D). It is a secondary active transport mechanism.

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QUESTION ID:68

Which of the following is not an asexual method of reproduction in fungi?

QUESTION ID:69

Which of the following forms of DNA is produced with a DNA sequence made up from alternating purine and pyrimidine nucleotides?

(A). B form

(B). Z form

(C). A form

(D). C form

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QUESTION ID:70

Match List-I with List-II

List-I (Technique)List-II (Description)
(A). Study to analyze pools of DNA from an environmental sample containing organisms that have not been isolated and identified(I). Proteomics
(B). Genome wide study of the structure, function and activity of organism's proteins(II). Metagenomics
(C). Global study of transcription is done by monitoring the total RNA generated under chosen growth conditions(III). Systems Biology
(D). The term used for integration of different fields of research to give an overview of an organism or a cell(IV). Transcriptomics
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QUESTION ID:71

Which of the following statements is/ are applicable to fixed angle rotors?

(A). During rotor acceleration, reorientation of the sample and gradient occur.

(B). Sedimentation and separation of the particles occur during centrifugation.

(C). Bands of separated particles appear when the rotor is at rest.

(D). The centrifuge tube is filled with gradient and then loaded with sample.

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QUESTION ID:72

Which of the following treaty/convention provided "international recognition of microorganisms" for the purpose of patent procedure?

QUESTION ID:73

B cell maturation in birds occurs in:

QUESTION ID:74

Which of the following statements is true about the lac operon?

(A). Lac operon is under positive regulation through CRP.

(B). Lac operon has three structural genes.

(C). Lac repressor binds to the lac promoter.

(D). Lac operon is expressed in the presence of both glucose and lactose.

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