Previous Year Questions

QUESTION ID:2

Root culture is used to produce: 

QUESTION ID:3

Synthetic seeds are:

QUESTION ID:4

Polyphenolic compounds are oxidized by polyphenol oxidases in the plants resulting in the darkening and spoilage of explants while performing tissue culture. The procedures used to overcome this issue include:

(A). Adding antioxidants to culture medium

(B). Presoaking explants in antioxidants

(C). Adding ascorbic acid in the media

(D). Frequently transferring explants into fresh media

Choose the correct answer from the options given below:

QUESTION ID:5

Endosperm culture is useful for the development of: 

QUESTION ID:6

Cytokinins:

(A). Are derivatives of adenines

(B). Are important for shoot induction

(C). Have property of cell elongation

(D). Usually promotes cell division if added together with auxin in culture

Choose the correct answer from the options given below:

QUESTION ID:7

Match List I with List II:

QUESTION ID:8

Match List I with List II: 

QUESTION ID:9

Match List I with List II: 

QUESTION ID:10

Match List I with List II: 

QUESTION ID:11

Shoot tip culture is performed through the following steps:

(A). Shoot tip collection from the plant

(B). Axillary shoot proliferation

(C). Rooting of shoots

(D). Primary culture of tip

Choose the correct answer from the options given below:

QUESTION ID:12

Different steps for regeneration via organogenesis:

(A). shoot formation

(B). callus formation

(C). Initial explant

(D). plantlet

Choose the correct answer from the options given below:

QUESTION ID:13

Following steps are used in Bergmann cell plating technique:

(A). Passing cells through gauge

(B). Mixing cells with molten agar medium

(C). Plating cells in petridishes

(D). Cell suspension

Choose the correct answer from the options given below:

QUESTION ID:14

In vitro techniques to produce haploids are used to generate:

QUESTION ID:15

The chromosome number of a haploid plant can be doubled using:

QUESTION ID:16

Different plant tissue culture methods used to produce haploids:

QUESTION ID:17

Development of haploid plants from male gametophytic tissue is known as:

QUESTION ID:18

When anthers containing microspores at the middle to late uninucleate stage are cultured on solid media then they form:

QUESTION ID:19

Microspores may give rise to the plants having:

QUESTION ID:20

Problem of mixoploidy associated with anther culture was overcome by:

QUESTION ID:21

Gynogenic embryogenesis can be developed by culturing:

QUESTION ID:22

Chromosome elimination technique was established for the production of:

QUESTION ID:23

For the development of diploid plantlets, the order of tissue culture steps will be:

(A). Development of haploid plantlet

(B). Collection of flowers

(C). Isolation and culture of anthers

(D). Colchicine treatment

Choose the correct answer from the options given below:

QUESTION ID:24

Different steps involved in the elimination of chromosomes in cereals through plant tissue culture:

(A). Embryo culture

(B). Chromosome elimination

(C). Crossing

(D). Monoploid plant development

Choose the correct answer from the options given below:

QUESTION ID:25

Homozygous lines developed through the anther culture must be analysed to check their ploidy status through:

(A). The colour of the flowers

(B). Counting of plastids in stomata

(C). Chromosome number

(D). Number of nucleoli

Choose the correct answer from the options given below:

QUESTION ID:26

Match List I with List II:

QUESTION ID:27

Most commonly used chemical for the protoplast fusion:

QUESTION ID:28

Protoplasts are:

QUESTION ID:29

Plant cell protoplasts can be prepared by treating cells with: 

QUESTION ID:30

Fluorescein diacetate (FDA) stain is used for: 

QUESTION ID:31

Agar in plant tissue culture acts as:

QUESTION ID:32

Following sequence is followed for the protoplast culture:

(A). Plant regeneration

(B). Treatment of cells with enzyme mixture

(C). Callus culture

(D). Leaf sterilization and removal of epidermis to expose mesophylls

Choose the correct answer from the options given below:

QUESTION ID:33

Different steps in the generation of somaclonal variation without in vitro selection:

(A). Explant selection

(B). Whole plant generation

(C). Callus formation

(D). Shoot generation

Choose the correct answer from the options given below:

QUESTION ID:34

Following are the disadvantages of somaclonal variation:

(A). Novel variants may arise

(B). Variations are cultivar dependent

(C). Most of the variations are of no apparent use

(D). Variations are not always stable and heritable

Choose the correct answer from the options given below:

QUESTION ID:35

The somatic hybrids produced through protoplast fusion needs authentication and purification due to very low efficiency of fusion, homofusion and no fusion, this may be performed by the following complementations:

(A). Alpha-complementation

(B). Chlorophyll deficiency complementation

(C). Auxotroph complementation

(D). Resistance marker complementation

Choose the correct answer from the options given below:

QUESTION ID:36

Match List I with List II: 

QUESTION ID:37

Match List I with List II: 

QUESTION ID:38

Match List I with List II: 

QUESTION ID:39

Match List I with List II: 

QUESTION ID:40

Nitrobacter spp involved in nitrogen cycle converts:

QUESTION ID:41

Nitrification is a process of conversion of:

QUESTION ID:42

Free living bacteria having nitrogenase converts atmospheric nitrogen to:

QUESTION ID:43

Nitrosomonas spp is useful for plants because it:

QUESTION ID:44

Leguminous plants have bacteria for:

QUESTION ID:45

Denitrification is carried by:

QUESTION ID:46

Nodules for the purpose of nitrogen fixation in non-leguminous plants are induced by:

QUESTION ID:47

Biological nitrogen fixation is performed by:

(A). Azotobacter and Beijerinckia

(B). Nostoc and Anabaena

(C). Rhizobium, Rhodospirillum, and Frankia

(D). Nitrobacter and Pseudomonas

Choose the correct answer from the options given below:

QUESTION ID:48

Biological nitrogen fixation is performed by some:

(A). Symbiotic bacteria

(B). Free-living anaerobic bacteria

(C). Cyanobacteria

(D). Free-living aerobic bacteria

Choose the correct answer from the options given below:

QUESTION ID:49

Match List I with List II: 

QUESTION ID:50

Match List I with List II: 

QUESTION ID:51

Nodule formation in plants happen through different steps:

(A). Plant cell differentiation

(B). Curling of the root hair

(C). Contact of Rhizobium with root hair

(D). Infection thread progression to cortex cells

Choose the correct answer from the options given below:

QUESTION ID:52

The systematic production of proteinase inhibitors in young tomato plants is triggered by a complex sequence of events:

(A). Synthesis of systemin

(B). Synthesis of prosystemin on the wound site

(C). Activation of several genes including proteinase inhibitors

(D). Synthesis of jasmonic acid

Choose the correct answer from the options given below:

QUESTION ID:53

The correct order of steps for developing a transgenic plant through plant tissue culture will be:

(A). Putative transgenic adult plant

(B). Regeneration on selection media

(C). Explant selection

(D). Addition of acetosyringone and Agrobacterium having transgene

Choose the correct answer from the options given below:

QUESTION ID:54

Which of the following molecule DOES NOT have an auxin-like activity?

QUESTION ID:55

Which of the following tissue would be most suitable for raising virus free plants?

QUESTION ID:56

During the organogenesis from the callus, relatively high ratio of BAP to IAA would be favoured for?

QUESTION ID:57

Which of the following is a carbohydrate, but CANNOT be used as carbon source for in vitro plant tissue culture?

QUESTION ID:58

Match the following plant tissue culture media components in list I with their functions:

QUESTION ID:59

In plant tissue culture, explants are dedifferentiated to form callus. Callus tissue can be regenerated into complete plantlets primarily by altering the concentration of: 

QUESTION ID:60

For ex-vitro rooting, shoots are treated with _____ and transplanted directly in the potting mix. Choose the correct option.

QUESTION ID:61

Colchicine is used for the production of:

QUESTION ID:62

Technique of anther culture was described for the first time by: 

QUESTION ID:63

Which of the following tissue would be most suitable for the development of haploids from a male sterile plant?

QUESTION ID:64

Which of the following statement is NOT TRUE for the haploid plants? 

QUESTION ID:65

Following are the various stages of a diploid plant generation from anthers:

(A). Homozygous diploid plants

(B). Explant preparation

(C). Differentiating callus

(D). Incubating anthers on culture media

(E). Colchicine treatment

(F). Haploid plantlets

Choose the correct answer from the options given below, that represent the events in sequencing order:

QUESTION ID:66

Frequency of albino production is high in?

QUESTION ID:67

Choose the correct combination of statements from the given list:

(A). Haploid plants regenerated through the callus culture would always be exactly similar to the parent plant.

(B). Production of haploids is very useful for developing homozygous lines that can be used for breeding research.

(C). Time taken to produce homozygous lines through doubled haploids is same as for selfing/inbreeding.

(D). Haploids have been successfully coupled with the breeding programs in several crops.

Choose the correct answer from the options given below:

QUESTION ID:68

Match List I with List II:

QUESTION ID:69

The first isolation of protoplasts by mechanical method was achieved by:

QUESTION ID:70

Water fern Azolla can be used as biofertilizer because: 

QUESTION ID:71

How many ATPs are required to generate four molecules of NH3 during symbiontic nitrogen fixation? 

QUESTION ID:72

Which of the following element play a key role in the process of biological nitrogen fixation in legumes?

QUESTION ID:73

Which of the following is a biological control method to control pests?

QUESTION ID:74

Which of the following organism can be used as a biocontrol agent in the treatment of plant diseases?

QUESTION ID:75

Nitrogen fixation by Rhizobium in nodule requires: