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Previous Year Questions

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TLS Online TPP Program

QUESTION ID:1

Viruses are

QUESTION ID:2

Who described microorganisms as 'wee animalcules' in his letter to Royal Society of London?

QUESTION ID:3

Which of the following statements is incorrect about the bacteria?

QUESTION ID:4

Match the following protozoa with their taxonomic groups:

List-I (Protozoa)List-II (Taxonomic Group)
(A). Entamoeba histolytica(I). Sporozoans
(B). Trypanosoma(II). Ciliophora
(C). Paramecium(III). Mastigophora
(D). Plasmodium(IV). Sarcodina
Choose the correct answer from the options given below:

QUESTION ID:5

Mushrooms and puffballs belongs to ________ class of fungi.

QUESTION ID:6

The plaque assay is used for quantification of 

QUESTION ID:7

Match List-I with List-II

List-IList-II
(A). Lichens(I). Protein coat called capsid protects nucleic acid
(B). Viroids(II). Completely lack cell wall and smallest living cell
(C). Bacteriophage(III). Good pollution indicators
(D). Mycoplasma(IV). A free RNA lacking protein coat
Choose the correct answer from the options given below:

QUESTION ID:8

Which of the following is not a mutagen? 

QUESTION ID:9

Which of the following kinds of organism/s in ecosystem is/are considered as decomposers? 

QUESTION ID:10

Which of the following is not used as seed inoculant for cereal crops? 

QUESTION ID:11

Recombinant DNA Technology for gene transfer has following sequence of steps

(A). Fragmentation of DNA by restriction endonucleases

(B). Isolation of desired DNA fragment and its ligation into a vector

(C). Isolation of DNA

(D). Transfer of recombinant DNA into a host and culture of host cells in a medium at large scale

Choose the correct answer from the options given below:

QUESTION ID:12

Which of the following is not an abiotic component of the ecosystem?

QUESTION ID:13

Number of Barr body/bodies found in XXY individual:

QUESTION ID:14

Arrange the following steps in formation of Pre-initiation complex in correct order

(A). TBP binds to TATA box and TFIIA binds DNA upstream of TATA box

(B). TFIIE then TFIIH bind forming the pre-initiation complex.

(C). TFIIB interacts with TBP

(D). RNAP II and TFIIF join the other proteins at the promoter.

Choose the correct answer from the options given below:

QUESTION ID:15

Which of the following interactions plays an important role in protein folding?

QUESTION ID:16

Enzymes accelerate the rate of cellular reactions by:

QUESTION ID:17

Match List-I with List-II

List-IList-II
(A). Alleles(I). an inherited character
(B). Trait(II). a particular segment of DNA responsible for the inheritance of a character
(C). Gene(III). two or more alternative forms of a gene
(D). Recessive(IV). an allele not expressed in the presence of dominant allele

Choose the correct answer from the options given below:

QUESTION ID:18

Test cross is a cross of F1 progeny with

QUESTION ID:19

A colourblind person cannot differentiate between

QUESTION ID:20

Match List-I with List-II 

List-I (Type of chromosome)List-II (Position of centromere)
(A). Metacentric(I). Chromosome has a terminal centromere
(B). Acrocentric(II). Chromosome has middle centromere forming two equal arms
(C). Sub metacentric(III). Centromere is present close to one end forming one very long and other very short arm
(D). Telocentric(IV). Centromere is present slightly away from the middle of the chromosome forming one shorter and other longer arm

Choose the correct answer from the options given below:

QUESTION ID:21

Match List-I with List-II

List-IList-II
(A). Syphilis(I). Mycobacterium
(B). Kala azar(II). Corynebacterium
(C). Tuberculosis(III). Leishmania
(D). Diphtheria(IV). Treponema

Choose the correct answer from the options given below:

QUESTION ID:22

Which of the following statement/s is true for cell membrane?

(A). It is composed mainly of lipids.

(B). Its fluid nature is important for cell growth, endocytosis, cell division etc.

(C). Fluid mosaic model of its structure was given by Griffith

(D). Its one of the important function is transport of molecules across it.

Choose the correct answer from the options given below:

QUESTION ID:23

Psychrophilic algae responsible for imparting the red coloration to snowfields is:

QUESTION ID:24

Crossing over occurs during ________ stage of prophase 1 of meiosis.

QUESTION ID:25

The sequence of phases in cell cycle is

(A). M phase

(B). G1 phase

(C). G2 phase

(D). S phase

Choose the correct answer from the options given below:

QUESTION ID:26

The lag phase in a bacterial growth curve does not occur under which of the following conditions? 

QUESTION ID:27

Choose the true statements about psychrophiles.

(A). Their enzymes have greater amounts of beta-sheet secondary structure.

(B). Their cytoplasmic membranes have higher content of unsaturated fatty acids.

(C). Their enzymes have greater polar amino acid content.

(D). Their transport proteins function at low temperatures.

Choose the correct answer from the options given below:

QUESTION ID:28

The capsid symmetry of poxviruses is:

QUESTION ID:29

Which of the following statements is correct? 

QUESTION ID:30

Which of the following is a professional APC?

QUESTION ID:31

For the separation of DNA by electrophoresis which of the following methods is used

QUESTION ID:32

Which pathway replaces TCA cycle when E. coli is grown on acetate as the only carbon source?

QUESTION ID:33

Dissimilatory nitrate reduction takes place under

QUESTION ID:34

The Wobble hypothesis refers to the less stringent base pairing specifity of the

QUESTION ID:35

GUG codes for valine in both prokaryotes and eukaryotes, but when GUG is initiation codon, it codes for

QUESTION ID:36

Arrange the following steps involved in bacterial transformation in correct order:

(A). Pairing followed by integration of transforming DNA

(B). Generation of a transformed cell.

(C). Binding and entry of extracellular DNA into the competent cell.

(D). Degradation of one strand of transforming DNA.

Choose the correct answer from the options given below:

QUESTION ID:37

Match List-I with List-II

List-I (Protein)List-II (Function)
(A). Trypsin(I). A hormone
(B). Collagen(II). Enables glucose transport into cells
(C). Insulin(III). An enzyme
(D). GLUT-4(IV). Intercellular ground substance

Choose the correct answer from the options given below:

QUESTION ID:38

The correct sequence of enzyme action is

(A). Binding of substrate induces the enzyme to alter its shape

(B). Binding of substrate to active site of an enzyme

(C). Breaking of chemical bonds of substrate and formation of new enzyme-product complex

(D). Products of reaction are released by enzyme.

Choose the correct answer from the options given below:

QUESTION ID:39

Select the correct statements with reference to cofactors of enzymes

(A). They are non-protein constituents of enzymes that bound to enzyme to make it catalytically active.

(B). Prosthetic groups are tightly bound to apoenzymes.

(C). The protein part of an enzyme is called apoenzyme.

(D). Coenzyme NADP contains vitamin niacin.

Choose the correct answer from the options given below:

QUESTION ID:40

Which of the following is not a function of the human microbiome?

QUESTION ID:41

A single nucleotide pair is inserted near the 5'-end of a protein coding DNA sequence. The most likely effect will be production of

QUESTION ID:42

When a mutational event leads to the replacement of one codon for another coding for the same amino acid, the resulting mutation is called

QUESTION ID:43

Match List-I with List-II

List-I (Mutagen/Agent)List-II (Function)
(A). Clastogen(I). causes fragmentation of chromosome
(B). Oncogen(II). induces tumor formation
(C). Teratogen(III). result in developmental abnormalities
(D). Ethyl methane sulphonate(IV). alkylating agent

Choose the correct answer from the options given below:

QUESTION ID:44

Which of the following statement/s is/are not true regarding significance of meiosis

(A). It is a mechanism by which conservation of specific chromosome number of each species is achieved across generations in sexually reproducing organisms.

(B). Increase genetic variability

(C). Decrease genetic variability

(D). Variations are not important for the process of evolution

Choose the correct answer from the options given below:

QUESTION ID:45

The primary target of HIV is

QUESTION ID:46

The sequence of steps for live recombinant vaccine production is

(A). Putting the cells in the presence of an inhibitor

(B). Transfection of newly created plasmid into previously infected host cells

(C). Formation of recombinant plasmid

(D). Expression of recombinant genes

Choose the correct answer from the options given below:

QUESTION ID:47

Which of the following is an inhibitor of complex IV of ETC?

QUESTION ID:48

Key enzyme of the ED pathway is:

QUESTION ID:49

Which of the following types of cheese utilize Lactococcus lactis in its early stages of production?

(A). Mozzarella

(B). Cheddar

(C). Roquefort

(D). Parmesan

Choose the correct answer from the options given below:

QUESTION ID:50

Which of the following is not a primary pollutant contributing to global air pollution?

QUESTION ID:51

The oxygen liberated during photosynthesis comes from

QUESTION ID:52

Match List-I with List-II

List-IList-II
(A). Rennin(I). PCR technology
(B). Protease(II). Coagulation of milk
(C). DNA polymerase(III). Household detergent
(D). Invertase(IV). Soft-center candies

Choose the correct answer from the options given below:

QUESTION ID:53

The sequence of transfer of electrons in Z scheme of light reaction is

(A). Downhill to NADP+ reducing it to NADPH + H+

(B). Excitation of electrons and transfer to another acceptor

(C). Through Photosystem II to uphill to the acceptor

(D). Down the ETS to Photosystem I

Choose the correct answer from the options given below:

QUESTION ID:54

Which of the following statements is not applicable to anoxygenic photosynthesis?

QUESTION ID:55

Which of the following is not a disaccharide?

QUESTION ID:56

Select the correct statement/s regarding Abscisic acid as a growth hormone

(A). It acts as general plant growth inhibitor.

(B). It is also called as stress hormone.

(C). It inhibits seed germination.

(D). It plays an important role in seed development, maturation and dormancy.

Choose the correct answer from the options given below:

QUESTION ID:57

Match List-I with List-II

List-IList-II
(A). Ustilago tritici(I). Crown gall disease
(B). Puccinia(II). Blight of potato
(C). Agrobacterium tumefaciens(III). Loose smuts of wheat
(D). Phytophthora infestans(IV). Leaf rusts

Choose the correct answer from the options given below:

QUESTION ID:58

Tetracyclin is:

QUESTION ID:59

Match List-I with List-II

List-I (Population interaction)List-II (Characteristics)
(A). Commensalism(I). When one species is harmed and other is unaffected
(B). Mutualism(II). When one species is benefitted and other is unaffected
(C). Amensalism(III). When both the species are benefitted
(D). Predation(IV). When one species kills the other

Choose the correct answer from the options given below:

QUESTION ID:60

Pyramid of energy in an ecosystem is always

QUESTION ID:61

Which of the following statements about linkage is incorrect?

(A). Genes linked on the same chromosome segregate together.

(B). Frequency of crossing over is proportional to the distance between two linked genes.

(C). Chromosome maps also provide information about linked genes.

(D). Linked genes assort independently.

Choose the correct answer from the options given below:

QUESTION ID:62

Which of the following is a left handed helix?

QUESTION ID:63

The species that invade the bare area are called

QUESTION ID:64

The sequence of steps for sewage treatment is

(A). Chlorination

(B). Screening and transfer to grit chamber

(C). Sedimentation and Aeration

(D). Anaerobic sludge digestion

Choose the correct answer from the options given below:

QUESTION ID:65

RNA Splicing is a process:

QUESTION ID:66

Which of the following enzymes is a high fidelity thermostable polymerase?

QUESTION ID:67

The Shine Dalgarno sequence plays an important role in

QUESTION ID:68

In a food chain man is

QUESTION ID:69

What would happen if phytoplankton of an ocean is destroyed for some reason?

(A). The ocean as carbon sink is adversely affected

(B). The ocean ecosystem is not affected

(C). The density of ocean is drastically decreased

(D). The food chains in the ocean are adversely affected

Choose the correct answer from the options given below:

QUESTION ID:70

The genotypic and phenotypic ratio of F2 generation is the same in which of the following situations?

QUESTION ID:71

Which of the following statements are correct about nitrogen fixation?

(A). Nitrogenase is the key enzyme for nitrogen fixation.

(B). The process involves conversion of nitrogen to nitrate.

(C). The process takes place in the presence of oxygen.

(D). Some plants can also fix nitrogen.

Choose the correct answer from the options given below:

QUESTION ID:72

Sodium dodecyl sulphate used in SDS-PAGE is

QUESTION ID:73

Which of the following are not fermentative food products?

(A) Dosa

(B) Kefir

(C) Kimchi

(D) Pasta

Choose the correct answer from the options given below:

QUESTION ID:74

The resistance of bacterial endospores to adverse environmental conditions is due to

QUESTION ID:75

Select the correct statements regarding thin layer chromatography

(A). It has two phases: one mobile phase and other stationary phase

(B). The relative movement of each component of a mixture is expressed as Rf value

(C). Spots of compounds which absorb iodine will show up as green spots

(D). The spots of colourless compounds are detected by putting the plate in UV light

Choose the correct answer from the options given below: