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QUESTION ID:1

Plants having half the somatic chromosome number than found in normal individual are called -

QUESTION ID:2

The production of haploids by anther and pollen culture was first demonstrated by -

QUESTION ID:3

Plants cannot absorb molecular nitrogen from the atmosphere because

QUESTION ID:4

Inducing the formation of various vegetative organs from cells or tissues in plant tissue culture is called -

QUESTION ID:5

Production of secondary metabolites require the use of -

QUESTION ID:6

Media that contain some chemical with unknown chemical composition is called ______ media.

QUESTION ID:7

For obtaining pure cultures of bacteria ______ plate method is not used.

QUESTION ID:8

Select the correct statements regarding somaclonal variations

A. These variations can also result in unwanted traits.

B. Somaclones have been developed and proved advantageous in several crops.

C. These variations can be used to engineer novel traits.

D. Short term invitro callus and cell suspension, cultures results in somaclonal variation.

Choose the correct answer from the options given below :

QUESTION ID:9

In plant tissue culture formation of organ primordia-like shoot or root in callus cells is called -

QUESTION ID:10

Who is regarded as father of 'Plant Tissue Culture' (P. T. C.)?

QUESTION ID:11

The first androgenic haploid plant product by anther culture was from -

QUESTION ID:12

The process of combining cytoplasmic genomes of one parent with nuclear genome of other parent is called

QUESTION ID:13

Molecules of ATP are required to fix one molecule of nitrogen (N2)to 2NH3

QUESTION ID:14

Symbiotic nitrogen fixing bacteria are found in -

A. Azolla

B. Gnetum

C. Anthoceros

D. Cycas

E. Riccia

Choose the correct answer from the options given below :

QUESTION ID:15

Important enzymes involved in nitrogen fixation are -

QUESTION ID:16

'Pomato' a hybrid of potato and tomato was produced through-

QUESTION ID:17

Arrange the following steps in the process of nitrogen cycle in correct sequence:

A. Nitrogen fixation

B. Nitrification

C. Denitrification

D. Assimilation

E. Ammonification

Choose the correct answer from the options given below :

QUESTION ID:18

Virus free plants can be grown by : -

QUESTION ID:19

Match LIST-I with LIST-II

LIST-ILIST-II
A. Somatic hybridizationI. Cell suspension culture
B. ParthenocarpyII. Fusion protoplasts from somatic cells
C. MicropropagationIII. Seedless fruits without fertilization
D. Single cell productionIV. Multiplication of plants without sexual reproduction
Choose the correct answer from the options given below:

QUESTION ID:20

Arrange the following steps for plant tissue culture (P.T.C.) in correct sequence:

A. Selection of desired material and suitable nutrient media for P. T. C.

B. Inoculation of explants

C. Surface sterilization of explant

D. Transfer of growing cultures

E. Transfer of plantlets to soil in pots

Choose the correct answer from the options given below :

QUESTION ID:21

In nitrogen cycle, Ammonification is the process of generating ammonia from -

QUESTION ID:22

Match LIST-I with LIST-II

LIST-ILIST-II
A. Gene inhibitionI. Addition of functional gene to their genome to replace missing product
B. Gene editingII. Disarm the product of faulty gene
C. Gene targetingIII. CRISPR/Cas 9
D. Gene Augmentation therapyIV. Replacement of non functional gene with normal gene
Choose the correct answer from the options given below:

QUESTION ID:23

Conversion of ammonia to nitrates is called -

QUESTION ID:24

Choose the correct statements regarding plant tissue culture

A. Organogenesis is inducing the formation of various vegetative organs from cells or tissues.

B. Formation of mass of undifferentiation cells from callus redifferentiation.

C. Cytoplasmic hybrids are prepared by taking the nucleus from one parent and cytoplasm from both the parents.

D. Relative concentration of growth hormones play important role in organogenesis.

Choose the correct answer from the options given below :

QUESTION ID:25

Full form of CRISPR, a term used in genome editing is -

QUESTION ID:26

Choose the correct statements regarding homozygous diploid plants -

A. They are produced by doubling the chromosome number of haploids.

B. Doubling of chromosome enables the recessive traits to express too.

C. Chromosome doubling is done by Ozone treatment.

D. Chromosome doubling is done by colchicine treatment.

Choose the correct answer from the options given below:

QUESTION ID:27

Transgenic plants developed by introducing Bt gene in crops like brinjal, maize, cotton etc. provide resistance to:

QUESTION ID:28

Which of the following converts nitrites to nitrates?

QUESTION ID:29

San Noeum first successfully cultured gynogenic haploid plants from unfertilized ovaries of -

QUESTION ID:30

Nitrogen fixation occurs with the help of symbiotic bacteria in

A. Pea

B. Lettuce

C. Beans

D. Tomato

E. Black gram

Choose the correct answer from the options given below:

QUESTION ID:31

In biological nitrogen fixation conversion of dinitrogen molecule into ammonia is carried out by ______ enzyme.

QUESTION ID:32

Choose the correct statements regarding cytology of haploids

A. A haploid in Arabidopsis will have 5 chromosomes.

B. Haploids are found as bivalents at metaphase-I of meiosis.

C. Haploids are found as univalent at metaphase-I of meiosis.

D. The haploids in maize will have 10 chromosomes.

E. The haploids in maize will have 20 chromosomes.

Choose the correct answer from the options given below:

QUESTION ID:33

Haploids can be artificially produced by -

A. Colchicine doubling

B. X-ray treatment

C. Pollen culture

D. Distant hybridization

E. Infrared radiation

Choose the correct answer from the options given below :

QUESTION ID:34

Autonomously replicating circular extrachromosomal DNA is called -

QUESTION ID:35

Nitrogen fixing cyanobacteria Anabaena is found in the root pockets of -

QUESTION ID:36

The cutting of DNA at specific locations became possible with the discovery of -

QUESTION ID:37

Arrange the basic steps to develop G. M. O. (Genetically modified organisms) in sequence:

A. Transfer of DNA with desired genes to its progeny

B. Identification of DNA with desired genes

C. Introduction of the DNA into the host.

D. Maintenance of introduced DNA in the host.

Choose the correct answer from the options given below :

QUESTION ID:38

microbial biocontrol agent which control butterfly caterpillars in plants is -

QUESTION ID:39

What is the function of leghemoglobin present in root nodulus of leguminous plants?

QUESTION ID:40

CRISPR-Cas 9 is a gene ______ technique.

QUESTION ID:41

Choose the correct statements regarding Agrobacterium tumefaciens -

A. It is a gram positive round shaped bacterium

B. It is a gram negative rod shaped bacterium

C. It is a photosynthetic spiral bacterium

D. It is also known as 'Natural genetic Engineer

E. It is capable of naturally transferring DNA into plant genome

Choose the correct answer from the options given below :

QUESTION ID:42

Source organism of cry genes is -

QUESTION ID:43

Which of the following elements play key role in nitrogen fixation?

QUESTION ID:44

Which of the following methods/tools is not used for introduction of recombinant DNA into host cell?

QUESTION ID:45

Arrange the following steps in the process of somatic hybridization in correct sequence:

A. Plating of fused protoplasts

B. Selection of hybrid cells

C. Protoplast isolation and its treatment with fusion chemical.

D. Transfer of callus to differentiation medium

E. Selection of somatic hybrid plants

Choose the correct answer from the options given below :

QUESTION ID:46

The correct combination of somaclonal variant released as a new cultivar is -

QUESTION ID:47

K. J. Kashaand and coworkers found that following the cross between Hordeum vulgare X Herdeum bulbosum chromosomes of H. bulbosum were eliminated in early zygotic division, so few days after pollination, embryos can be cultured to get haploids. This method is called as -

QUESTION ID:48

Antisense RNA technique is used -

QUESTION ID:49

Using natural predators for the control of pathogens is known as ______ control.

QUESTION ID:50

Optimum pH for protoplast culture is -

QUESTION ID:51

Match LIST-I with LIST-II

LIST-I (Culture Type)LIST-II (Use/application)
A. Embryo cultureI. Somatic hybridization
B. Meristem cultureII. Production of haploids
C. Protoplast cultureIII. Shortening of breeding cycle
D. Anther cultureIV. Virus free plants
Choose the correct answer from the options given below:

QUESTION ID:52

Prions are the -

QUESTION ID:53

What is CPW in protoplast culture method?

QUESTION ID:54

Arrange the following events in Western Blotting experiment in correct order:

A. Protein resolution by PAGE

B. Primary antibody binding

C. Transfer onto nitrocellulose membrane

D. Protein denaturation in loading dye.

Choose the correct answer from the options given below :

QUESTION ID:55

In cyanobacteria nitrogen fixation takes place in

QUESTION ID:56

Synthetic seeds are produced by the encapsulation of somatic embryos with-

QUESTION ID:57

In plant tissue culture higher concentration of cytokinin generally promotes-

QUESTION ID:58

Chemical most widely used for chromosome doubling in haploid culture is-

QUESTION ID:59

Which combination of strategies forms the basis of in vivo haploid induction technologies in plants?

A. induction of parthenogenesis

B. culture of anthers or ovules

C. use of paternal inducer lines

D. uniparental genome elimination

Choose the correct answer from the options given below:

QUESTION ID:60

Match LIST-I with LIST-II

LIST-ILIST-II
A. Biological nitrogen fixationI. Nitrobacter
B. Conversion of ammonia to nitrite.II. Paracoccus
C. Conversion of nitrite to nitrateIII. Rhizobium
D. DenitrificationIV. Nitrosomonas
Choose the correct answer from the options given below:

QUESTION ID:61

Select the correct combination of protoplast isolation enzyme and its most popular source

QUESTION ID:62

Match LIST-I with LIST-II 

LIST-I (Biocontrol)LIST-II (Example)
A. BacteriumI. Bacillus thuringiensis
B. FungusII. Clostridium
C. InsectIII. Trichoderma
D. BiopesticideIV. Cotton aphid
Choose the correct answer from the options given below:

QUESTION ID:63

Copies of DNA strands generated during a polymerase chain reaction are known as

QUESTION ID:64

Which gene in shoot apical meristem (SAM) negatively regulate WUS expression?

QUESTION ID:65

Match LIST-I with LIST-II  

LIST-I (Organism)LIST-II (use in biotechnology)
A. Thermus aquaticusI. Cry proteins
B. Agrobacterium tumefaciensII. DNA polymerase
C. E. coli DH5aIII. epspr gene
D. Bacillus thuringiensisIV. DNA cloning
Choose the correct answer from the options given below:

QUESTION ID:66

Match LIST-I with LIST-II   

LIST-I (Technique)LIST-II (used for)
A. Northern blottingI. To detect specific proteins in this sample of tissue homogenate
B. Southern blottingII. Detection of specific post translation modification of proteins.
C. Western blottingIII. To detect specific RNA molecule in mixture of RNA.
D. Eastern blottingIV. To detect specific DNA in a mix of samples.
Choose the correct answer from the options given below:

QUESTION ID:67

In general, the cytoplasmic male sterility (CMS) causing genes are transcribed in which plant cell organelle?

QUESTION ID:68

Match LIST-I with LIST-II  

LIST-ILIST-II
A. Karl ErekyI. Invented DNA 'Finger-printing
B. Joshua LederbergII. Coined the term 'Biotechnology'
C. Kary MullisIII. Discovered plasmids
D. Sir Alec JefferysIV. Developed polymerase chain reaction
Choose the correct answer from the options given below:

QUESTION ID:69

Match LIST-I with LIST-II   

LIST-ILIST-II
A. ProtoplastI. Ability of cell to develop into a new plant
B. ExplantII. Unorganized mass of cells
C. TotipotencyIII. Naked cell
D. CallusIV. Any plant tissue used to regenerate new tissue/organ/plant in vitro
Choose the correct answer from the options given below:

QUESTION ID:70

Match LIST-I with LIST-II  

LIST-ILIST-II
A. AuxinI. undifferentiated mass of cell
B. ProtoplastII. 6-Furfuryl amino purine
C. CallusIII. Indole 3 Acetic Acid
D. CytokininIV. Pectinase
Choose the correct answer from the options given below:

QUESTION ID:71

Match LIST-I with LIST-II  

LIST-ILIST-II
A. GynogenesisI. Callus culture
B. Culturing in liquid mediumII. Ovary culture
C. AndrogensisIII. Suspension culture
D. Culturing on agar mediumIV. Pollen culture
Choose the correct answer from the options given below:

QUESTION ID:72

Which of the following will be present in the F1 multicellular embryo, derived from a cross of female plant (A) with male plant B, through "bulbosum method"?

QUESTION ID:73

Arrange the steps in PEG induced protoplast fusion in correct sequence -

A. Treatment of protoplast mixture with 28-50% PEG for 15-30 minutes.

B. Protoplast aggregation.

C. Washing of protoplast (alkaline medium pH 9-10), and high Ca2+ concentration

D. Selection of protoplasts of different strains / species.

Choose the correct answer from the options given below:

QUESTION ID:74

Match LIST-I with LIST-II  

LIST-I (Scientist)LIST-II (Landmark discovery)
A. Van OverbeekI. Transgenic Bt-cotton
B. WhiteII. Introduced coconut water as a media component
C. WentIII. First synthetic plant tissue culture medium
D. MonsantoIV. First plant growth hormone ie IAA
Choose the correct answer from the options given below:

QUESTION ID:75

Match LIST-I with LIST-II  

LIST-ILIST-II
A. Sea weedsI. Isolation of DNA from gel.
B. Staining of DNAII. Gel electrophoresis
C. ElutionIII. Source of agarose
D. Separation of DNA fragmentsIV. Ethidium bromide
Choose the correct answer from the options given below: