Previous Year Questions

QUESTION ID:2

Which of the following will be the part of the pellet, when tissue cells are homogenized and centrifuged at 1000g for 10 min.?

QUESTION ID:3

Arrange the following DNA fragments A, B, C, D separated after Gel Electrophoresis in increasing order of number of their base pairs.

QUESTION ID:4

Which of the following is not true about gel electrophoresis?

QUESTION ID:5

Which of the following are the types of column chromatography?

A. Paper chromatography
B. Affinity chromatography
C. Size exclusion chromatography
D. Ion-exchange chromatography

Choose the correct answer from the options given below:

QUESTION ID:6

Tryptophan, tyrosine and phenylalanine absorb ultraviolet light. Therefore, the protein rich in these amino acids strongly absorbs the light of wavelength _________ due to aromatic side chain of these amino acids.

QUESTION ID:7

A scientist wants to separate three protein molecules by ion-exchange chromatography. The pH of the mobile phase of the column is maintained in such a way that the protein molecule (A) has a net charge of −2, protein molecule (B) has a net charge of +2 and protein molecule (C) has a net charge of +1. Which one of the molecule/s will elute first from a cation-exchange resin?

QUESTION ID:8

A technique for transferring denatured DNA molecules that have been separated electrophoretically from a gel to a matrix (such as a nitrocellulose or nylon membrane) on which a hybridization assay can be performed is called

QUESTION ID:9

The DNA of the bacterial cell is protected from the cell's own restriction enzymes by the addition of methyl group (–CH₃) to ______ of cytosine.

A. 4th Carbon
B. 7th Carbon
C. 6th Carbon
D. 5th Carbon

Choose the correct answer from the options given below:

QUESTION ID:10

Which of the following statements are correct regarding prokaryotic genomic organization?

A. Most prokaryotes contain a single large circle of double stranded DNA as its genome.
B. The nucleoid is an irregularly shaped region that contains the cell's chromosome and numerous proteins.
C. Nucleoid associated proteins (NAPs) are not particularly important during cell division for the compaction of chromosome.
D. Prokaryotic genomes are much smaller as compared to eukaryotes.

Choose the correct answer from the options given below:

QUESTION ID:11

In humans, one of the most recognized satellite DNA sequences, found mainly in the centromere region is:

QUESTION ID:12

Select correct statement about Euchromatin:

QUESTION ID:13

Match the LIST-I with LIST-II

QUESTION ID:14

Telomerase acts as a cellular reverse transcriptase that provides the active site for:

QUESTION ID:15

Which of the following molecule/ion is not transported/buffered by hemoglobin? 

QUESTION ID:16

Which of the following pair of class and organism is not correctly matched? 

QUESTION ID:17

Which one of the following statement is incorrect regarding the work of Erwin Chargaff? 

QUESTION ID:18

Which of the following amino acids are essential amino acids in humans:

A. Leucine

B. Lysine

C. Isoleucine

D. Serine

Choose the correct answer:

QUESTION ID:19

Match the LIST-I with LIST-II 

QUESTION ID:20

The glycosidic bond between the monomers of sucrose is-

QUESTION ID:21

Match the LIST-I with LIST-II 

QUESTION ID:22

Arrange the steps of recombinant DNA-cloning procedure in correct sequence of their occurrence:

A. Joining the target DNA with cloning vector DNA by ligase enzyme.
B. Targeted DNA from a source organism is cleaved and cloning vector DNA is also cleaved by the same restriction endonuclease.
C. Selection of transformants from non-transformants through antibiotic resistance and color indicators.
D. Introduction of recombinant DNA into host cell.

Choose the correct answer from the options given below:

QUESTION ID:23

Tetracycline is usually mixed with the culturing medium as selective agents in selecting transformants in gene cloning technique. It acts by:

QUESTION ID:24

Match the LIST-I with LIST-II 

QUESTION ID:25

For sterilization, the culture media and glasswares must be generally autoclaved at:

QUESTION ID:26

Benzoic acid or benzoates are used to preserve food from:

A. Insects
B. Yeast
C. Clostridia
D. Molds

Choose the correct answer from the options given below:

QUESTION ID:27

The Bdellovibrio bacterium is a _____ bacteria:

A. Gram negative
B. Predator of gram negative bacteria
C. It has a single polar flagellum
D. Reproduce by producing spores called myxospore during nutrient deficit conditions

Choose the correct answer from the options given below:

QUESTION ID:28

Organisms belongs to the genus Bacteroides are:

QUESTION ID:29

A method for culturing anaerobes is the GasPak System, which uses hydrogen and a ______ catalyst to remove O₂.

QUESTION ID:30

The antimicrobial agent mercuric chloride functions by

QUESTION ID:31

Match the LIST-I with LIST-II 

QUESTION ID:32

The Ti plasmid and its T-DNA have been genetically modified for use as a vector for the insertion of recombinant DNA into plant chromosomes to prevent dicots from tumor diseases. The source of Ti plasmid is:

QUESTION ID:33

The ability of a molecule to function as an antigen depends on its:

A. Size
B. Structural complexity
C. Chemical nature
D. Degree of foreign nature to the host

Choose the correct answer from the options given below:

QUESTION ID:34

Match the LIST-I with LIST-II 

QUESTION ID:35

The cationic antimicrobial peptide, histatin is found in human’s ________:

QUESTION ID:36

Convalescent sera belongs to

QUESTION ID:37

Typhoid Vi antigen type of vaccine is:

QUESTION ID:38

Which one of the given statement is not correct about plant viruses?

QUESTION ID:39

Hepatitis C virus can cause liver cancer. It has:

QUESTION ID:40

The role of slow sand filters in water treatment facilities is: 

QUESTION ID:41

Match the LIST-I with LIST-II 

QUESTION ID:42

The F₁ hybrid (Rr Ii) is crossed with a variety double recessive for both the traits. How many types of zygotes will be produced in the cross?

QUESTION ID:43

Which of the following statement is wrong about C₄ plants? 

QUESTION ID:44

The plants bloom when the light duration is less than 12 hours per day are know as:

QUESTION ID:45

A rock body through which ground water flows is called: 

QUESTION ID:46

Match the LIST-I with LIST-II 

QUESTION ID:47

The left-handed helix, Z-DNA is 18 Å (1.8 nm) in diameter which contains ______.

QUESTION ID:48

Choose the correct sequence of events as they occur during a bacterial transcription

A. RNA polymerase sigma (σ) subunits recognizes promoters
B. DNA double helix unwind
C. Sigma (σ) subunit dissociates from the holoenzyme
D. Formation of hairpin secondary structure

Choose the correct answer from the options given below:

QUESTION ID:49

Choose the correct answer regarding the translation of mRNA to proteins when AUG is the start codon and codes for methionine.

QUESTION ID:50

Choose the correct sequence of different regions on a typical eukaryotic DNA segment when we go from the upstream to the downstream of a transcription start point.

A. Initiation site
B. CAAT box
C. Enhancer
D. TATA box

Choose the correct answer from the options given below:

QUESTION ID:51

Which one of the following acid is hydrophobic with non polar side chain?

QUESTION ID:52

Choose the correct order of steps involved in nucleotide excision repair of damaged bases as they occur.

A. A fresh burst of DNA synthesis
B. Cuts are made on both the 3′ side and the 5′ side of the damaged area
C. The DNA is unwound producing a “bubble”
D. A DNA ligase covalently binds the fresh piece into the backbone

Choose the correct answer from the options given below:

QUESTION ID:53

Match the LIST-I with LIST-II 

QUESTION ID:54

Frequency of recombination between linked genes in Drosophila is affected by

A. Distance between genes
B. Sex of heterozygotes for linked genes
C. Age of female
D. Temperature

Choose the correct answer from the options given below:

QUESTION ID:55

Match the LIST-I with LIST-II 

QUESTION ID:56

In general F₂, F₃ and the subsequent generations do not show segregation for a cytoplasmically inherited trait. This is because

QUESTION ID:57

Which of the given factors can affect frequency in Mendelian population?

A. Migration
B. Mutation
C. Selection
D. Random shift

Choose the correct answer from the options given below:

QUESTION ID:58

In 1963, several varieties such as Sonalika and Kalyan Sona were introduced all over the wheat-growing belt in India. They were

QUESTION ID:59

Among the following species of bryophytes, which is not an example of mosses?

QUESTION ID:60

Who among the following is known as the father of Indian paleobotany?

QUESTION ID:61

The symmetry in which a flower can be divided into two equal and similar halves by only one vertical division is:

QUESTION ID:62

In homosporous pteridophyte species, the development of gametophyte is

QUESTION ID:63

More recently discovered plant hormone, Jasmonates, play an important role both in plant defense and development, is derived from which fatty acid?

QUESTION ID:64

Plants can assimilate only two inorganic forms of nitrogen. These are:

A. Nitrite (NO^2-)
B. Ammonium (NH⁴⁺)
C. Nitric acid (HNO₃)
D. Nitrate (NO_3-)

Choose the correct answer from the options given below:

QUESTION ID:65

Lenticels permit the:

QUESTION ID:66

The membrane potential of a resting neuron is:

QUESTION ID:67

Which one of the following amino acids is involved in synthesis of hormone epinephrine in humans?

QUESTION ID:68

Match the LIST-I with LIST-II 

QUESTION ID:69

Which one of the following granulocyte has following features?

Highly phagocytic; nucleus with three to five lobes; primary and secondary granules; limited life span.

QUESTION ID:70

Which of the following are autosomal recessive genetic disorder?

A. Huntington disease
B. Sickle cell anemia
C. Lesch–Nyhan syndrome
D. Tay–Sachs disease

Choose the correct answer from the options given below:

QUESTION ID:71

Which one of the following proteins is often referred to as “the guardian of the genome”?

QUESTION ID:72

Colibactin is a DNA-damaging compound and when taken up by human intestinal cells, colibactin results in double-stranded DNA break and can cause human colorectal cancers. It is synthesized by:

QUESTION ID:73

Agar is used for preparing solid media for culture. Which of the following is not true about agar media?

QUESTION ID:74

Which one of the following groups of animals is devoid of gill slits?

QUESTION ID:75

Which one of the following is not a tetrapod character?