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QUESTION ID:1

In which one of the following PCR assays only one primer is used for amplification?

QUESTION ID:2

Gaps in certain regions of the genome have been observed upon sequencing of a xerohalophyte. Which one of the following databases will NOT be of any use in filling up these gaps?

QUESTION ID:3

One of the most popular genes used for developing rice tolerant to flooding stress is:

QUESTION ID:4

“Refugia” is a practice commonly employed to control

QUESTION ID:5

Oat seeds will usually NOT germinate when exposed to Red (R) and Far red (FR) light in the following order:

QUESTION ID:6

Vir genes are necessary for the transfer of the T-DNA into the host genome. The product of which one of the following genes is tightly associated with the 5′ end of the T-strand and helps in nuclear targeting?

QUESTION ID:7

A microorganism following Monod kinetics is grown in a chemostat with working volume of 5 L and inlet substrate concentration of 1g/L. If the μmax and Ks of the organism are 0.5 h-1 and 0.25g/L respectively, washout occurs when the flow rate (L.h-1) exceeds

QUESTION ID:8

How does the rate of a typical chemical reaction vary as a function of temperature?

QUESTION ID:9

An enzyme follows Michaelis-Menten kinetics with the following parameters: Vmax= 5 mM/s and Km= 2.5 mM. The reaction velocity would be:

QUESTION ID:10

A high cell density fermentation produces recombinant protein product which is 20% of the total cellular protein. The final cell density is 80 OD (1 OD = 0.4g of dry cell wt/L of which 50% is total cellular protein). Then the product concentration (g/L) is:

QUESTION ID:11

In a plug flow bioreactor running at steady state, 12.5% cells are recycled back to the inlet. If the cells grow at μmax=1 h-1 throughout the length of the reactor (L=124 cm), then the flow velocity should be

QUESTION ID:12

In a CSTR, first order reaction takes place converting A to B. If at a dilution rate (D)= 0.5 h-1, 50% of A gets converted to B, then the rate constant ‘k’ of the reaction is:

QUESTION ID:13

In a continuous crystallizer, 100 g of a saturated solution of sugar at 85% (w/w) enters the crystallizer and leaves the crystallizer at 70% (w/w). The weight of input solids converted to crystals (g) in the crystallizer is

QUESTION ID:14

We wish to produce a metabolite ‘X’ whose biosynthetic pathway is feedback inhibited in the wild type strain. A mutation, which leads to overproduction of X, is discovered in the gene coding for a homodimeric enzyme which catalyses the first step in the biosynthetic pathway of ‘X’. This mutation is most likely to occur at:

QUESTION ID:15

A new spherical resin (of radius 300 urn) has been developed in which only the outer layer is activated to a depth of 100 um (as shown in figure). The fraction of the activated volume is: 

QUESTION ID:16

In a fed batch cultivation, a specific growth rate of 0.2/h needs to be maintained. At the start of fedbatch cultivation, 200 mL/h of media is fed to 1000 mL of working volume. Quasi steady state shall be obtained if:

QUESTION ID:17

A recombinant protein is produced in Escherichia coli by two stage continuous cultivation at steady state. Upon induction of the culture in stage II, the specific growth rate of the culture decreased considerably. For an input flow rate in stage I of 100mL/h, a steady rate of product formation can only be obtained

QUESTION ID:18

Consider the scale up of a fermentation from a 10 L to 10,000 L while maintaining geometric similarity. Agitation speed was maintained at 500 rpm in the 10 L fermenter. If scale up is done based on constant tip speed, then the agitation speed in the larger reactor should be ………….rpm

QUESTION ID:19

A culture can grow independently in two carbon sources, glucose and hexadecane. Identify which one of the following statements is TRUE?

QUESTION ID:20

In mammalian cell culture based monoclonal antibody production, perfusion culture is preferred over continuous stirred tank culture, because the process results in:

QUESTION ID:21

A liquid stream is cooled from SOT to 30*C in a double pipe heat exchanger as illustrated below: 

Fluid flowing counter currently with this stream is heated from 15°C to 30°C. Calculate the log mean temperature difference. 

QUESTION ID:22

In a typical fermentation process the volumetric oxygen transfer coefficient (KLa) of the system was found to increase after the addition of antifoam agent. The most probable reason for this is:

QUESTION ID:23

In a batch microbial fermentation process the dissolved oxygen concentration (DOC) remains almost zero during growth. Which of the following methods will you use for the estimation of oxygen transfer rate while the fermentation is in progress?

QUESTION ID:24

Two columns of 1 m and 2 m height and of equal diameter are packed with beads containing immobilized enzyme. Substrate was fed to these two columns at flow rates of 10 mL/min (1 m column) and 20 mL /min (2 m column) and the corresponding conversion efficiencies obtained were 30% and 40% respectively. This demonstrates that the reaction is:

QUESTION ID:25

A food package label displays composition of the food product in g/100g as: protein = 8g, fat= 20g, carbohydrate= 60g (of which sugar is 20g). The calorific value of this product before and after complete replacement of sugar (w/w) by a non-calorific sweetener would be:

QUESTION ID:26

Two sucrose solutions ‘A’= 30g/100g and ‘B’= 60 g/100 g have to be mixed to prepare 1 kg of 50g/100g sugar syrup. The amounts of ‘A’ and ‘B’ solutions to be mixed would be:

QUESTION ID:27

A food product having water activity of 0.6 is exposed to conditions of 30°C and 70% R.H. This product will tend to ___________

QUESTION ID:28

The driving force for mass transfer by molecular diffusion is the difference in

QUESTION ID:29

Sedimentation efficiency depends on the relative strength of :

QUESTION ID:30

In anaerobic lactic acid fermentation by bacteria, glucose is partially oxidised to pyruvate followed by reduction to lactate:

QUESTION ID:31

Which one of the following is NOT used for partition based bio-separation?