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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

While analyzing protein sequences of rpoB gene among different bacteria, different changes in amino acid sequences were observed among the homologs due to base substitution. If transition is more frequent than transversion, single mutation is more frequent than double mutation, which one of the following changes will occur most frequently than others?

QUESTION ID:2

If G2/M check point and DNA repair proteins are working optimally in a cell and there are some DNA damages during G2 phase, which one of the following would be the response of the cells to damage?

QUESTION ID:3

Transferrin binds with its receptor and recruits clathrin via which one of the following proteins?

QUESTION ID:4

Which one of the following is the major product of fatty acid synthase?

QUESTION ID:5

Intracellular transport in mammalian cells through vesicular fusion is regulated by which among the following GTPases?

QUESTION ID:6

Which one of the following processes is NOT regulated by the kidney?

QUESTION ID:7

Rapid removal of large quantities of blood will cause an animal to go into hypovolemic shock and may even cause death. If the weight of a mouse is 20 gms, what is the amount of maximum blood one can withdraw from a single bleed to prevent the mouse from going into hypovolemic shock?

QUESTION ID:8

In order to have a desired shRNA cassette integrated in target cells, which of the following gene transfer vectors is preferable?

QUESTION ID:9

Which one of the following statements regarding naturally occurring Agrobacterium strains is true?

QUESTION ID:10

The integration of T-DNA in the plant nuclear genome is most likely due to

QUESTION ID:11

Variation in transgene expression levels among independent transgenic events generated using the same transgene construct could be due to
(i) position effects
(ii) strength of the promoter used to express the transgene
(iii) variation in copy number of the transgene
(iv) presence of restriction enzyme sites in the transgene sequence

QUESTION ID:12

A typical flowering plant has a combination of sporophytic (diploid) and gametophytic (haploid) tissues. Which of the following types of plant tissues do NOT contribute to the formation of a mature fruit?

QUESTION ID:13

The submerged leaves of an aquatic plant are feathery (to avoid damage due to flowing underwater currents) while leaves on the surface are padded (to help in floatation). The correct statement related to the above phenotype is

QUESTION ID:14

Which one of the following statements related to the development of insect-resistant transgenic plants is correct?

QUESTION ID:15

When the gene and the promoter used for modification of a plant using transformation technology are derived from sexually compatible species, the modified plant thus generated is known as a

QUESTION ID:16

A transgenic plant segregates in a 3:1 ratio for the transgenic: nontransgenic phenotype in T1 progeny derived by self-pollination. This indicates that

QUESTION ID:17

Which one of the following reporter genes can be used for real-time visualization of living cells/tissues in transgenic plants?

QUESTION ID:18

Which of the following statements represent correct features of sexual and asexual modes of reproduction in flowering plants?
(i) In sexual reproduction, progeny are genetically different from each other.
(ii) In asexual mode of reproduction, progeny are genetically identical to each other but different from the parent.
(iii) Development of greater adaptive ability is possible only in case of progeny derived by sexual reproduction.
(iv) A minor change in the habitat may adversely affect all offspring derived by asexual reproduction.

QUESTION ID:19

Which one of the options given below represents the correct combination of plant defense responses listed in Column A and Column B?

QUESTION ID:20

Non-conditional, negative selection marker genes are usually expressed under a

QUESTION ID:21

A method in which a strong enhancer is randomly inserted in a plant genome by transformation, resulting in mutant plants with dominant phenotypes, is known as

QUESTION ID:22

Which one of the following organisms is used by plant biologists to study translocation in phloem?

QUESTION ID:23

In a genetic transformation experiment, a researcher failed to add the antibiotic selection agent in the shoot regeneration medium for selection of transgenic plants. In the absence of any other confounding factors, which one of the following statements is expected to be correct?

QUESTION ID:24

Orientation of a cloned passenger gene cassette in a binary plasmid vector, containing the expression cassette of a selectable marker gene, can be checked by PCR using

QUESTION ID:25

Somatic hybrid plants with full or nearly full complement of the two parental species are called

QUESTION ID:26

In CAGE,

QUESTION ID:27

Which one of the following statements about associative nitrogen fixing bacteria is NOT true?

QUESTION ID:28

According to the Seeds Act of 1966, which one of the following functions is NOT applicable to a Seed Certification Agency?

QUESTION ID:29

Which one of the combinations given below represents populations with a similar genetic composition?

QUESTION ID:30

Which one of the following statements represents a correct definition of Gene Pyramiding?

QUESTION ID:31

A marker closely linked to the gene of interest for an agronomic trait will be used for

QUESTION ID:32

The vitrification of cultured explants is generally caused by

QUESTION ID:33

The Rht mutations in wheat that were pivotal for 'Green Revolution' reduce plant height due to impairment in

QUESTION ID:34

Steady state for a closed system implies

QUESTION ID:35

In a lab scale reactor, the size of the air bubbles in the reactor is primarily determined by

QUESTION ID:36

Generally fungal fermentations are pseudoplastic. Hence in large reactors the cells

QUESTION ID:37

Mixing time increases with the volume of the reactor because of increase in the

QUESTION ID:38

The Reynolds number is a ratio of

QUESTION ID:39

The equation τ = μ du/dy is also termed as

QUESTION ID:40

Match the Transport process with appropriate time constants given below.

Where V is volume; Q is pumping rate; N is agitation rate; D is diameter of the impeller; A is area and ρ is the density of the liquid

QUESTION ID:41

When a liquid of density 1200 kg/m3 and viscosity 0.01 kg m-1s-1 flows through a 1 cm ID pipe at 0.2 m/s, the flow is referred as a

QUESTION ID:42

In a dynamic gassing out technique for determination of KLa in a bioreactor, upon restart of the aeration the increase in DO slows down with time because

QUESTION ID:43

Under unaerated conditions in a fermenter, the power consumed by a single impeller is 10 KW and upon changing the agitation rate from 200 to 600 rpm, the new power consumption (KW) would be -----. (Assuming that the power number remains constant)

QUESTION ID:44

Fill in the blanks with appropriate option: Vinegar is a liquid consisting of acetic acid produced by the fermentation of _______________ by _____________________.

QUESTION ID:45

A food stored in a refrigerator has caused an outbreak of food borne illness. Which one of the following is most likely to be the pathogen responsible for this?

QUESTION ID:46

To reduce the tartness in wine due to malic acid, a secondary fermentation is carried out using

QUESTION ID:47

Which one of the following statements is INCORRECT with respect to food rheology?

QUESTION ID:48

Which one of the following enzyme(s) would result in high glucose yield from starch hydrolysis?

QUESTION ID:49

The major metabolite formed and the succession of microorganisms responsible for Sauerkraut fermentation are

QUESTION ID:50

Omega 3 fats are good for health and play an important role in prevention of heart disease. If you have to formulate a food product rich in omega 3 fats which one of the following options would you choose?

QUESTION ID:51

For a well-established enzyme assay method where the reaction kinetics should remain zero order, what percentage of substrate is converted to product during the incubation period?

QUESTION ID:52

As the viscosity increases, the method used for mixing is changed in the following order

QUESTION ID:53

On scale up, the effective area available for heat transfer in a bioreactor

QUESTION ID:54

A fermenter produces 100 kg lysine per day. If the volumetric productivity is 0.8 gl-1h-1, what is the volume of the fermenter in liter?

QUESTION ID:55

Chemostat cultivation is carried out at steady state with a dilution rate of 0.1 h-1 . Given μm = 1.1 h-1and Ks=0.1 g/L. What will be steady state substrate concentration?

QUESTION ID:56

For degradation of crude oil in ocean by microorganisms, the two limiting nutrients are

QUESTION ID:57

The first circumpolar expedition in the history of Marine Sciences was

QUESTION ID:58

Paralytic shellfish poisoning (PSP) is caused by the consumption of molluscan Shellfish contaminated with

QUESTION ID:59

Biofilm/capsular material produced by the soil bacteria is detected by

QUESTION ID:60

A compound secreted by a soil bacterium capable of reducing the surface tension at air-water interface is called as

QUESTION ID:61

Match the organism in Group A with the appropriate habitat from Group B.

Group A                                   Group B

P) Pseudomonas                  i) Hot springs

Q) Thermus                           ii) Rhizosphere

R) Photobacterium               iii) Soil

S) Thiobacillus                      iv) Marine

QUESTION ID:62

Match the type of Organism in Group A with appropriate phenotype/property from Group B.
Group A                                                   Group B
P) Pseudomoans aeruginosa             i) Sulfur oxidizing organism
Q) Staphylococcus aureus                ii) Elemental N2 fixation
R) Thiobacillus ferroxidance            iii) Biofilm formation
S) Bradyrhizobium                            iv) Quorum sensing

QUESTION ID:63

Which one of the following reactions is performed by Cytochrome-P450 to detoxify the xenobiotics?

QUESTION ID:64

Which one of the following is NOT true regarding Zika virus?

QUESTION ID:65

The vaccine currently used in India’s Pulse polio campaign is

QUESTION ID:66

Which one of the following enzymes helps in the survival of Helicobacter pylori in the stomach?

QUESTION ID:67

Which one of the following is INCORRECT about Streptokinase?

QUESTION ID:68

The utility of positron emission tomography to diagnose tumors is based on

QUESTION ID:69

Which one of the following imaging techniques is non-invasive?

QUESTION ID:70

Amphotericin B is clinically used against which one of the following pathogens?

QUESTION ID:71

The therapeutic index (TI) indicates the relative margin of safety of a drug and it is deduced from the values of mean lethal dose (LD50) and median effective dose (ED50). Which of the following represents TI of a drug?

QUESTION ID:72

Which one of the following is a frequently used radio-opaque dye?

QUESTION ID:73

The name of the blood vessel marked [?] in the figure below is


QUESTION ID:74

Which one of the following is a reason for administering general anesthesia to experimental animals?

QUESTION ID:75

During the pathogenesis of rabies, the replication of virus first starts in the

QUESTION ID:76

Callipyge gene mutation in sheep leads to increased muscle growth. The gene presents a typical case of Polar over-dominance. Which of the four genotypes given below shows increased muscle growth? (N= wild type allele, C= mutant allele for callipyge)

QUESTION ID:77

The technique used in animal biotechnology for the rapid multiplication and production of animals with a desirable genotype is:

QUESTION ID:78

A primary culture of neurons isolated from the mouse brain responded positively when exposed to a chemical for a few seconds, but the neurons were less responsive and eventually stopped responding when exposed to the same chemical for prolonged duration. However, if the culture was left for some time and washed, the neurons started responding to the same chemical at the same concentration as before. The reduced response was due to

QUESTION ID:79

If a neuron is tyrosine hydroxylase positive, it could be either:

QUESTION ID:80

Pain sensation is a subjective and conscious feeling. However, although the autonomic organs viz. brain, heart etc. do not get represented in the cerebral cortex, one feels pain in those parts as well. The reason is

QUESTION ID:81

Neurons are formed from precursor proneural cells due to

QUESTION ID:82

A neuron at resting state when treated with “X” showed transmembrane potential -50mV, while when treated with “Y” it showed -90mV. Given such a condition, which of the following statements would be most appropriate?

QUESTION ID:83

Route of entry for canine distemper virus is

QUESTION ID:84

In a population which is in Hardy-Weinberg equilibrium, the frequency of a recessive allele for a certain genetic trait is 0.40. What percentage of individuals would be expected to show the dominant trait in the next generation?

QUESTION ID:85

Color blindness in human is a sex-linked recessive trait. If two individuals with normal color vision have a color blind son, what will be the genotypes of the parents?

QUESTION ID:86

A human is phenotypically female, but her interphase somatic nuclei do not display the existence of Barr bodies. Which of the following conditions could explain the above feature?

QUESTION ID:87

For the pedigree depicted below, which mode(s) of inheritance CANNOT be possible?

QUESTION ID:88

C banding of human chromosomes specifically reveals

QUESTION ID:89

Which one of the following expressions is likely to retrieve more matches in a database search?

QUESTION ID:90

Which substitution matrices should you prefer to find distantly related orthologs through BLAST search?

QUESTION ID:91

Match the items in Group 1 with an appropriate description in Group 2
Group I                                      Group 2
P. UPGMA                         1. Protein sequence database
Q. CLUSTALW                  2. Phylogenetic Analysis
R. SWISS-PROT              3. 3-D structure visualization
S. RasMol                         4. Multiple sequence alignment

QUESTION ID:92

The rate of substitutions in a certain region of DNA of length 1000 bases is estimated as 10-9 per base per year. If two species diverged approximately 10 million years ago, the fraction of sites that differ between them should be approximately

QUESTION ID:93

When searching the Blocks and PRINTS databases, a match is judged significant if

QUESTION ID:94

Two species are found to share a cluster of 8 genes, but the genes are in different orders in the two species. The orders are represented by signed permutations as given below:
Species X 1,2,3,4,5,6,7,8
Species Y 1, 2,-5,-4,-3,8,6,7
The transformation between the two gene orders

QUESTION ID:95

As the E value of a BLAST search becomes smaller

QUESTION ID:96

In a genome with average GC-content 40%, the expected frequency of occurrence of the tetranucleotide AACG is

QUESTION ID:97

Consider that you have collected X-ray diffraction data on three different protein crystals, referred to as Protein A, B and C. Data set for crystal A has the best diffraction spot at 8A, crystal B at 5A and crystal C has the best diffraction at 3A. Which data set is likely to yield a higher resolution crystal structure?

QUESTION ID:98

In a protein crystallization experiment, you have identified an optimal protein concentration and precipitant concentration which yield crystals. Now if you are asked to grow crystals again with a protein solution that is four times more concentrated, how will you alter the precipitant concentration?

QUESTION ID:99

Which of the following statements is true for two different tripeptides consisting of either glycine or proline?

QUESTION ID:100

In a Fluorescence Resonance Energy Transfer (FRET) experiment, the donor is Cyan Fluorescent Protein (CFP) which has excitation and emission maxima at 435 and 485 nm, respectively. The acceptor in this experiment is Yellow Fluorescent Protein (YFP) that has excitation and emission maxima at 513 and 545 nm, respectively . Under the conditions, where a significant FRET is observed, what will happen to the FRET signal if the emission maxima of CFP is made to shift to 475nm.

QUESTION ID:101

Consider that the following polypeptides are being analyzed by Mass spectrometry (MS). These peptides are generated by proteolytic cleavage of a protein that is expressed in mammalian cells and it has undergone glycosylation, phosphorylation and acetylation. What will the expected positioning of the spectra corresponding to these peptides (smaller to larger)?

QUESTION ID:102

Assuming that the sequence of CDRs of an antibody are heavily enriched with Tyrosine and Serine, what is likely to be the driving force stabilizing its interaction with the antigen?

QUESTION ID:103

Hydrophobic amino acids are prevalent in transmembrane regions of membrane embedded proteins because

QUESTION ID:104

Bryostatin is an anticancer agent obtained from

QUESTION ID:105

Synthesis of White spot syndrome virus envelop takes place in

QUESTION ID:106

Primary etiology of Epizootic Ulcerative Syndrome is a Fungus Aphanomyces invadans. Its zoospores have the property to

QUESTION ID:107

Gonad Inhibiting Hormone (GIH) in crustaceans is synthesized in

QUESTION ID:108

The synthetic equivalent of neuro-pharmacologically active peptides obtained from the marine snail Conus magus is

QUESTION ID:109

Viral encephalopathy and retinopathy in fishes are caused by

QUESTION ID:110

A pigment isolated from marine red algae that finds application in flow cytometry is

QUESTION ID:111

Which of the following electron acceptor that is used by the bacteria is mainly responsible for marine corrosion?

QUESTION ID:112

Which one of the following bacterial species is responsible for the Scombroid fish poisoning?

QUESTION ID:113

The larval stage of Macrobrachium rosenbergii is referred to as

QUESTION ID:114

The group of marine algae which have urea cycle is

QUESTION ID:115

Which of the following algae is responsible for the red color of RED SEA?

QUESTION ID:116

An oligotrophic lake has

QUESTION ID:117

After sodium chloride, which one of the following compounds has the maximum concentration in sea water?

QUESTION ID:118

Which of the following is an example of an intellectual property right?