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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

The fluid property, due to which, mercury does not wet the glass is

QUESTION ID:2

A major organism used in commercial bioleaching for copper recovery is

QUESTION ID:3

A covalently closed circular DNA was in a relaxed state in water at 300C. What will happen if the water temperature increases to 600C or decreases to 100C?

QUESTION ID:4

Pruning helps in making the hedge dense because

QUESTION ID:5

The location of a proteins in cells can be studied using

QUESTION ID:6

A fermentation medium is being cooled from 700C to 320C in a double pipe heat exchanger. Fluid flowing counter currently with this stream in heated from 200C to 460C. The log mean temperature difference (in0C) for the two streams is

QUESTION ID:7

In a DNA molecule, two antiparallel strands that are complementary in their nucleotide sequence are paired to form a

QUESTION ID:8

Consider a bacterium that grows with doubling time of 20 min in the exponential phase of its growth cycle and acquire 10 random mutrations in its genome in every generation. How many mutations will it acquire after 48 hr of growth in exponential phase?

QUESTION ID:9

During genome engineering process, the role of flippase enzyme in the next round of modification in the target gene is to

QUESTION ID:10

An energy generatioin process in which organic compounds act as both electron donors and terminal electron acceptor in a microbe is called

QUESTION ID:11

Which of the following process does NOT contribute to conversion of a proto-oncogene to oncogene?

QUESTION ID:12

The artificial sweetener, aspartame, is enzymatically produced using

QUESTION ID:13

The sequence CGAATTTGG is matched globally with four sequences in a database. The sequence that will give the highest similarity score taking match=1, mismatch=0 and gap penalty=minus1 is

QUESTION ID:14

In which organelle of seeds are stored oils converted to fatty acids and glycerol during germination?

QUESTION ID:15

Met-Ile-Val-His-Tyr was the sequence of a hypothetical peptide. Assuming that there are two possible codons each for His, val and Try, one possible codon for Met, and four possible codons for Ile, the number of possible nucleotide sequences coding for this peptide would be

QUESTION ID:16

What is the use of Aminopterin in hybridoma production?

QUESTION ID:17

How many linkage groups would be there in a plant with 2n=20?

QUESTION ID:18

A bacterial population, growing in batch culture, increases from 1000 cells to 10,00,000 cells in 5 hours. What is the generation time of the bacteria?

QUESTION ID:19

In large scale fermentation processes, corn steep liquor is mainly used as a

QUESTION ID:20

Aqueous two phase partitioining (ATPS) is used for the recovery of an enzyme from the cell free culture fitrate. On addition of PEG-2000 and dextran, the mixture separates into two phases with a partition coefficient for the enzyme as 4.2. The maximum possible enzyme recovery, when the volume ratio of the upper to lower phases in 5.0, will be

QUESTION ID:21

Which interactions are generally observed at the core of stable protein-protein complexes?

QUESTION ID:22

The genetic disease familial Hypercholesterolemia that leads to an increase in blood cholesterol is caused due to

QUESTION ID:23

If four atoms A, B, C, and D are connected linearly and there is rotation possible along bond BC, dihedral angel on this bond is described as

QUESTION ID:24

Computational Prediction of protein folding assumes that

QUESTION ID:25

The Budapest Treaty related to the international patent process concern with

QUESTION ID:26

What is agent Orange?

QUESTION ID:27

Match the polysaccharides (L.H.S.) with the microbial cultures (R.H.S.) associated with their industrial production

QUESTION ID:28

The comparison of the structures of haemoglobin and myoglobin shows that they have

QUESTION ID:29

All the vaccines mentioined below are attenuated or inactivated whole pathogen except

QUESTION ID:30

A combination of gametes that can be formed by the genotype AaBbCcDdEeFfGg are

QUESTION ID:31

Which of the following microorganism is used for commercial production of dextran?

QUESTION ID:32

dilution of a microbial culture was prepared by adding 1 ml of the culture to 9 ml of sterile blank. Further, 200 𝝁𝑳 from the diluted culture was spread on an agar plate; and 150 colonies were observed after the incubation period. Calculate the CFU/mL of the original sample

QUESTION ID:33

At what condition does the specific growth rate of the microorganisms decline in a constant volume fed-batch culture

QUESTION ID:34

Cyclosporine, as immunosuppressive drug, given to avoid transplant rejection acts by

QUESTION ID:35

Which of the following is not a suitable material for a depth filter used in air sterilization?

QUESTION ID:36

A protein with 1000 amino acids was tagged with GFP. The molecular weight of GFP is 26 KDa What will be the most likely molecular weight of the fused target protein?

QUESTION ID:37

The temperature (℃) of liquid nitrogen used for cryopreservation of plant samples is

QUESTION ID:38

Which of the following methods first ionizes a protein before separation and detection?

QUESTION ID:39

The first organic acid to be produced industrially is

QUESTION ID:40

In the design of a fermenter, which one of the following is NOT the intended use of baffles?

QUESTION ID:41

Which one of the following statements is true regarding the magnesium porphyrin ring and the phytol chain of a chlorophyll molecule?

QUESTION ID:42

Synthetic seeds are mostly derived from

QUESTION ID:43

A supramolecular complex that serves to degrade damaged or unneeded proteins in the cell is called as

QUESTION ID:44

A mixture of three proteins (X, Y, and Z) was loaded on a size exclusion column. The molecular weight (<W) and pl of the proteins are as follows

QUESTION ID:45

Baroreceptors are responsible for sensing human

QUESTION ID:46

ATP with 𝜸−𝟑𝟐p can be used for which of the following type of reaction?

QUESTION ID:47

Epicatechin gallate (ECG) is a type of flavonoid found in which of the following?

QUESTION ID:48

What are the cellular sites for protein glycosylation?

QUESTION ID:49

'Cybrids' are produced by

QUESTION ID:50

Two proteins of molecular weights 1.0 x 105 and 1.0 x 104 Daltons were eluted from a gel filtration column at 220 ml and 300 ml respectively. The molecular weight of an unknown protein that elutes at 140 ml under the same conditions, will be

QUESTION ID:51

Sequence-specific recognition of DNA by proteins occurs primarily through the

QUESTION ID:52

Choose the correct order in terms of pka

QUESTION ID:53

Which of the following is NOT a feature of bacterial DNA replication?

QUESTION ID:54

A student added a 5' exonuclease enzyme instead of a restriction enzyme to digest his purified plasmid DNA sample. What is he likely to observe when he runs his plasmid digest on an agarose gel?

QUESTION ID:55

A CDNA encoding a human protein of interest was cloned in a bacterial expression vector and introduced into bacterial cells for expression. However, no expression of the human protein of interest was obtained. This could be because of

QUESTION ID:56

Only 10 % of babies with Edward syndrome survive beyond 5 years. This is a genetic disease arising due to

QUESTION ID:57

Which autoimmune disease is caused by production of autoantibodies and autoreactive T cells against DNA and chromatin proteins?

QUESTION ID:58

Immobilization of enzymes to water insoluble, anionic porous carriers often results in an apparent shift in the pH optima of the enzyme. The physico-chemical interaction likely to cause such a behaviour is

QUESTION ID:59

Nucleosides isolated from a Caribbean sponge, Cryptotethya crypta, were the basis for the synthesis of the antiviral

QUESTION ID:60

What is Single Nucleotide Polymorphism (SNP)?

QUESTION ID:61

Accuracy of Protein Structure Prediction can be assessed using tools like

QUESTION ID:62

A class of temperature sensitive E. coli mutants defective in DNA replication were identified that ceased replication immediately upon increase in temperature. Which of the following processes are likely to be defective in these mutants?

QUESTION ID:63

A set of closely linked genetic markers present on a single chromosome, which are not easily separable by recombination and tend to be inherited together are termed as

QUESTION ID:64

Cellulose is a linear polymer of glucose with

QUESTION ID:65

The specific energy source for the reaction ADP + phosphate → ATP by the enzyme ATP synthetase (CF1 Coupling Factor) in thylakoid membranes is

QUESTION ID:66

Which of the following is NOT used for producing vitamins industrially?

QUESTION ID:67

In Transmission Electron Microscope (TEM), a beam of electrons interact with the specimen to form image as

QUESTION ID:68

Which one of the following approaches is generally not used for identifying an SNP?

QUESTION ID:69

DNA glycosylases are DNA repair enzymes involved in

QUESTION ID:70

What will be the molarity of a 4 mg/ml solution of NaOH?

QUESTION ID:71

A humanised antibody is one in which the

QUESTION ID:72

Identify the incorrect pair

QUESTION ID:73

Genes related through vertical descent from a common ancestral gene are called

QUESTION ID:74

Which of the following is NOT used as a biopesticide?

QUESTION ID:75

Which of the following does not have a quaternary structure?

QUESTION ID:76

The technique used to locate specific genes in chromosomes

QUESTION ID:77

Which is the correct arrangement of the polarity of solvents?

QUESTION ID:78

A protein of 100 KDa would have approximately how many amino acids?

QUESTION ID:79

Which of following is a trisaccharide?

QUESTION ID:80

The first stable product of C3 cycle is

QUESTION ID:81

Which of the following yeast is used for the production of riboflavin?

QUESTION ID:82

Ananda Chakrabarty received the first U.S. patent for a GM organism. This organism was

QUESTION ID:83

Deficiency of this macronutrient causes older leaves to turn dark green or reddish purple