Menu

Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

With reference to lac operon which one of the following merodiploids will show a constitutive expression of β-galactosidase?

QUESTION ID:2

In what way are the homeotic genes of flowering plants similar to those of Drosophila?

QUESTION ID:3

Integrin signaling promotes cell migration by inducing changes in the

QUESTION ID:4

Photon capturing prosthetic group moiety of chlorophyll molecule is known as

QUESTION ID:5

Opsonization process is involved with

QUESTION ID:6

Poly-L-leucine solution in dioxane is

QUESTION ID:7

Which one of the following is best suited for hydrolyzing peptide bonds on the carboxyl side of aromatic residues?

QUESTION ID:8

Which one of the following molecules has the highest diffusion coefficient in plasma membrane?

QUESTION ID:9

Cystic Fibrosis Transmembrane Regulator (CFTR) Protein is a

QUESTION ID:10

Which one of the following modifications targets a protein for degradation?

QUESTION ID:11

Regulation of blood glucose level is by

QUESTION ID:12

Which of the proteins or polysaccharides to which fibronectins are capable of binding is a normal plasma membrane component?

QUESTION ID:13

The observation that plasma membrane proteins mix after cell fusion provides evidence for

QUESTION ID:14

The treatment of hepatocytes in in vitro culture with Ricin leads to

QUESTION ID:15

In a gel filtration chromatography

QUESTION ID:16

Two dimensional gel electrophoresis is a technique for separating proteins

QUESTION ID:17

Exact mass and sequence of proteins and peptides can be measured by

QUESTION ID:18

Fluorescence microscopy is based on the ability of certain molecules to

QUESTION ID:19

An IgG and an IgM samples (against human red blood cell surface antigen) were treated with β- mercaptoethanol independently and tried for agglutinating human red blood cells with a view to checking the hemagglutination titer of the two samples. Which one of the following results is correct in this context?

QUESTION ID:20

A lectin can bind to plasma membrane vesicles if it is

QUESTION ID:21

Membrane asymmetry can be regulated by

QUESTION ID:22

What is the activated reactant in the biosynthesis of phosphatidylinositol from inositol?

QUESTION ID:23

Best method in tracking the synthesis and maturation of a protein in a living cell is

QUESTION ID:24

Fluorescence recovery after photobleaching (FRAP) is a powerful technique for calculating

QUESTION ID:25

Treatment of the inside-out vesicles with an uncoupler like DNP that allows protons to freely move across the vesicle membrane would be expected to

QUESTION ID:26

The concept of “magic bullet” proposed long back for targeted anti-cancer drug delivery is not a reality till today because

QUESTION ID:27

One of the major reasons of the failure of somatic gene therapy protocol can be assigned to

QUESTION ID:28

SRP selectively recognizes ER signal sequences on newly synthesized proteins. This is an outcome of

QUESTION ID:29

In the active site of Transaldolase, which amino acid forms the Schiff base?

QUESTION ID:30

An enzyme has an activation energy of 10,700 cal/mole. How many times faster (approximately) will the reaction proceed at 37 oC compared to 15 oC?

QUESTION ID:31

Identify the small protein (167 amino acids) that is produced in adipocytes and acts on receptors in the hypothalamus to curtail appetite

QUESTION ID:32

A TLC run of rat liver phospholipids is sprayed with ninhydrin, and the colour is allowed to develop. Which phospholipids can be detected in this way?

QUESTION ID:33

N-ethylmaleimide (NEM) is known to selectively inhibit bacterial lactose transporters. Which amino acid of the transporter is responsible for this inhibition?

QUESTION ID:34

Highest turnover number of an enzymatic reaction so far known is exhibited by

QUESTION ID:35

The chemical nature of covalent linkage in a disaccharide is known as

QUESTION ID:36

Cancer cells have an unique property to exhibit uncontrolled division. This is primarily due to

QUESTION ID:37

To evaluate cytotoxic potential of any anti-cancer drug, which is the molecule of choice?

QUESTION ID:38

A monoclonal antibody binds to G-actin but NOT to Factin. What does this tell you about the epitope recognized by the antibody?

QUESTION ID:39

Glutathione is

QUESTION ID:40

Which one of the following amino acids is optically inactive?

QUESTION ID:41

Protein responsible for transport of O2 from alveoli to the tissue is

QUESTION ID:42

Process of formation of ATP from ADP during photosynthesis is referred to as

QUESTION ID:43

Glycogen and cellulose are

QUESTION ID:44

Topoisomerase I does not require ATP even though it does cleavage and ligation of DNA. This is because

QUESTION ID:45

A synthetic mRNA of repeating sequence 5'- CACACACACACACACAC... is used for a cell-free protein synthesizing system like the one used by Nirenberg. If we assume that protein synthesis can begin without the need for an initiator codon, what product or products would you expect to occur after protein synthesis?

QUESTION ID:46

You have obtained a sample of DNA, and you transcribe mRNA from this DNA and purify it. You then separate the two strands of the DNA and analyze the base composition (%) of each Aland and of the mRNA. You obtain the data shown in the table below. Which strand of the DNA is the coding strand, serving as a template for mRNA synthesis? 

 

  A

 G

C

T

U

DNA strand 1

 39.1

26.0

31.0

14.9

0

DNA strand 2

 14.2

30.8

25.7

38.3

0

mRNA

 39.0

25.9

30.8

0

14.6

QUESTION ID:47

Which one of the following tools of recombinant DNA technology is INCORRECTLY paired with its applications? 

QUESTION ID:48

Among the six types of plant cation channels identified, the shaker channels have been most thoroughly characterized. The shaker channels are highly selective to which one of the following cations?

QUESTION ID:49

The process of oxidation of plastohydroquinone (PQH2) molecule during light reaction of photosynthesis takes place in which of the following major protein complexes of the thylakoid membrane?

QUESTION ID:50

In a metabolic engineering experiment the flux from primary carbon metabolism was diverted towards methylerythritol phosphate (MEP) pathway. Which one of the following secondary metabolites will be maximally synthesized?

QUESTION ID:51

Which one of the following sequence of events is the first to take place during systemic production of protease inhibitors in young tomato leaves following insect feeding?

QUESTION ID:52

Which of the following is NOT a typical feature of hypersensitive response observed in plants during the attack of invading microbes?

QUESTION ID:53

Plants carrying a transgene for glyphosate resistance will survive a field application of glyphosate that kills weeds. Which of the following enzyme is inhibited by glyphosate?

QUESTION ID:54

A haploid sperm from one species and a haploid egg from another species may form a diploid interspecies hybrid. Meiosis in these plants generally fails but can
lead to rare duplicated gametes called

QUESTION ID:55

Genes essential for embryo organization have been identified by mutant analysis. Which one of the following mutants shows reduction or inhibition of cotyledons and shoot apical meristem?

QUESTION ID:56

Which one of the following is true for the polymertrapping model of sugar diffusion in plant cells?

QUESTION ID:57

The T-DNA of Agrobacterium must be excised from its circular plasmid for its transfer into plant cells. Which one of the following complexes of Vir proteins is responsible for DNA nick at the left and right border sequences?

QUESTION ID:58

What will happen to the growth of a vertically growing root if half of the root cap is removed?

QUESTION ID:59

GA2-oxidase cDNA from bean is overexpressed in wheat plant by genetic engineering. Which one of the following phenotypes correctly describes the resultant transgenic plant?

QUESTION ID:60

Which one of the following statements is NOT correct regarding ethylene signaling in plants?

QUESTION ID:61

Leaf abscission is a phenomenon regulated by the amount of auxin and ethylene. Which one of the following statements is correct regarding the amount of auxin and ethylene during leaf abscission?

QUESTION ID:62

Transgenic plants were developed with a gene conferring krummycin resistance. Four independent Te events were seeted and — 100 T1 seeds were genniutted on kmuunyein. The number of kanamyein sensitive (Flans) and kanamycin resistant (ElanR) progeny obtainer' in each case is listed below: 

Identify the line where the Transgenic has Integrated at a single locus. 

QUESTION ID:63

Using transgenic technology male sterile plants are developed by

QUESTION ID:64

One of the concerns in commercial usage of transgenic plants is the spread of transgene through pollen flow. Which one of the following methods can be used to circumvent this problem?

QUESTION ID:65

Which one of the following can be used as a selection marker for developing transgenic plants?

QUESTION ID:66

Ac transposon is a /an

QUESTION ID:67

SSR is said to be a --------------type of marker

QUESTION ID:68

Direct DNA uptake by protoplasts can be stimulated by

QUESTION ID:69

Aroma in rice is due to

QUESTION ID:70

The male sterile cytoplasm in pearl millet is

QUESTION ID:71

The pathogen against which the crop rotation can be adopted

QUESTION ID:72

Iodine test is used in the detection of

QUESTION ID:73

When urea-denatured inclusion bodies are renatured, protein aggregates can sometimes be found due to misfolding. For small proteins with no disulphide linkages and a single domain, the probability of misfolding is the highest when

QUESTION ID:74

The net charge of a protein may not be sufficient to determine whether a protein will bind to an ion exchanger. This is due to

QUESTION ID:75

In a large scale high cell density fed batch reactor, which one of the following statements is true?

QUESTION ID:76

Optimum bead loading for cell disruption in a bead mill is

QUESTION ID:77

In which one of the following membrane separation processes, concentration difference is the driving force?

QUESTION ID:78

Expanded bed chromatography runs on the principle of

QUESTION ID:79

Which one of the following statements pertaining to ultrafiltration is INCORRECT?

QUESTION ID:80

Which one of the following factors affects the morphology of fungal biomass the least?

QUESTION ID:81

In order to fractionate particles based on size; which of the following centrifuges is best suited?

QUESTION ID:82

Which one of the following purification steps always requires a high initial ionic strength in the sample?

QUESTION ID:83

In Sephadex G series (gel filtration) as the G number increases from 10, 15, 25….200, the pore size of the beads

QUESTION ID:84

In a large scale bioreactor with fungal fermentation, the dissolved oxygen concentration gradient is primarily due to the

QUESTION ID:85

When in a plug flow reactor with low levels of axial mixing, is the maximum product concentration obtained?

QUESTION ID:86

The reasons for existence of lag phase in a batch cultivation process in a bioreactor could be due to the following reasons. Identify the best combination from the following.P: low inoculum size Q:low preinoculum size R:stationary phase inoculum S:inoculum not having the same medium as in batch reactor

QUESTION ID:87

Increasing aeration at a particular rpm does not always lead to increase in oxygen mass transfer in a bioreactor. This is because

QUESTION ID:88

If in a fed batch cultivation of recombinant E. coli, feeding of the substrate is based on the following equation F(t)= [μVX(t)]/SF.YXS. Where μ is specific growth rate and is maintained constant, F(t) is feed rate, X(t) biomass cell concentration,YXS is biomass yield coefficient and SF is the substrate feed concentration. The feed pattern indicates that with increasing time, every cell is receiving the

QUESTION ID:89

Match the KLa determination method in Group I with the characteristics of the method in 1 Group II

P.

oxygen balance method

 1.  

aeration of an actively respiring culture is stopped and switched on again

Q

static gassing out technique

 2.

yields greater OTR and KLa than other methods

R.

dynamic gassing method

 3.

It can be done precisely only at steady state

S.

sodium sulphite oxidation method

 4.

Non respiring biological system is required

QUESTION ID:90

Under unaerated condition in a fermentor, the power consumed by a single impeller was 10,000 W. The diameter of the impeller D is 1m and the density of the medium is 1000 kg/m3 and the stirrer speed is 1s-1. If one more impeller is added to the fermentor, power consumption would be approximately

QUESTION ID:91

For mixing solid contents in a media mixing tank, inclined blade turbine is used because it

QUESTION ID:92

In microbial cultivation experiment with cells having significant maintenance requirements, the measured growth yield Y’ XS (G) and the true growth yield Y XS

QUESTION ID:93

The logistic equation for growth of a bacterial culture is given by 
Where X and Xo are biomass concentration, k and beta are constants. The model depicts that initially when x is small, the growth pattern of the culture would be 

QUESTION ID:94

In an anaerobic fermentor, broth is agitated intensely at 100rpm in a 3.0 m3 baffled tank using a Rushton turbine with diameter of 0.5m. If the diameter of the
impeller is decreased to 0.25m the mixing time in (seconds) would approximately increase ………fold

QUESTION ID:95

Culture comprising a B. subtlis strain by inoculating 1g of cells into 1 liter bioreactor. Fermentor containing 10g/l of glucose. The maximum specific growth rate of the culture is 1.0 /h. The biomass yield from glucose is 0.5 g/g. If the residual glucose concentration left out is 1g/l, the final cell concentration in g/l achieved would be around

QUESTION ID:96

Continuous stirred tank fermenter is characterized by (biomass concentration X kg/m3, residual substrate is S kg/m3, Sf=substrate concentration in feed kg/m3, Dilution rate = D and μm>>D and Km<Sf, XD= the biomass productivity kg/m3h. If the dilution rate is doubled to 2D then the new steady state values in comparison with earlier steady state is characterized by

QUESTION ID:97

Fourier'; law of heat conduction
 
This equation is very similar to 

QUESTION ID:98

A fluidized bed is formed when

QUESTION ID:99

Batch filtration often characterized by continuous increase of the inlet pressure of slurry, is called the ……………filtration

QUESTION ID:100

The most precise method of estimating the metabolic heat produced in an aerobic fermentation process is by

QUESTION ID:101

The ethanol yield per unit mass of substrate consumed (YP/S ) in an anaerobic fermentation process can be improved by

QUESTION ID:102

Exponentially growing cells increase 10 fold in 10 hours, the time required to increase 5 fold will be ………hours

QUESTION ID:103

There are five classes of immunoglobulins [Ig]: IgM, IgG, IgD, IgA and IgE. Upon stimulation of B cells through B cell receptor and a switch factor, which one of the following Ig classes does NOT switch?

QUESTION ID:104

For quantitation of an antigen, researchers A, B, C and D used the following set-up. Which one of the following set up is the most sensitive?

QUESTION ID:105

A mouse deficient in ROR-gammaT will be deficient in

QUESTION ID:106

The specificity in IFN-α/β induced response comes from

QUESTION ID:107

CD152-deficient mice have lymphoproliferative disorder. The disorder is characterized by

QUESTION ID:108

MHC class-II molecule can signal because it

QUESTION ID:109

The T cells in CD28-deficient mice do not proliferate in response to re-exposure to the same antigen. This is because

QUESTION ID:110

CD1 molecules are unique antigen-presenting molecules because they

QUESTION ID:111

Mutations in common gamma chain of receptors for IL-2 cytokine family cause

QUESTION ID:112

Which one of the following conditions generally favours tumour development?

QUESTION ID:113

Which one of the following is NOT a single gene disease?

QUESTION ID:114

Dental plaque is an example of the formation of

QUESTION ID:115

Rheumatic fever is a complication of

QUESTION ID:116

Undulant fever is a characteristic of

QUESTION ID:117

Bubonic plague is the infection of

QUESTION ID:118

Leptospirosis is transmitted by exposure to animal

QUESTION ID:119

Which one of the following is correct when Na ion concentration in the bathing fluid of a squid giant axon is less than that present in sea water, but the osmotic pressure is maintained by substituting it with choline chloride?

QUESTION ID:120

When Tetrodotoxin is administered in bathing medium of a nerve fibre during voltage clamp experiments the membrane current may show which one of the following changes?

QUESTION ID:121

The arrival of the action potential in a presynaptic knob initiates quantal release of neurotransmitter by influx of Ca ions, which may act by

QUESTION ID:122

Which one of the following changes will occur when the external K+ ion concentration in the neuromuscular junction of skeletal muscles is increased?

QUESTION ID:123

The black widow spider venom, alpha-latrotoxin produces its neurotoxicity by

QUESTION ID:124

The generalized seizure is due to the activity of

QUESTION ID:125

Which one of the following genes is related with the late-onset of Alzheimer’s disease?

QUESTION ID:126

The first genetic maps that were developed used genes as markers because

QUESTION ID:127

In E. coil four Hfr strains donate the following genetic markers, shown in the order donated 
All these HFr strains are derived from the same F4 strain. What is the order of these markers on the circular chromosome of the original Ff? 

QUESTION ID:128

Which of the following statements is true?

QUESTION ID:129

In which one of the following evolutionary processes, random changes in allele frequency can lead to a loss of genetic diversity?

QUESTION ID:130

In human pointed eyebrows are dominant to smooth eyebrows and widow’s peak (downward pointed frontal hairline) is dominant to continuous hairline. What phenotypic ratio would you expect in the offspring from a cross between an individual heterozygous for both genes and an individual homozygous recessive for both genes?

QUESTION ID:131

The character regulated by a holandric gene

QUESTION ID:132

Necrosis that develops in tissues subsequent to denaturation of structural and enzymatic proteins soon after death is appropriately referred to as

QUESTION ID:133

The demyelination of the central nervous system white matter produced by the canine distemper virus is an example of

QUESTION ID:134

Catarrhal exudate is observed in

QUESTION ID:135

Sudden onset of heavy mortality in chicken with cyanotic comb and wattles, closed eyes and a semicomatose state are characteristic features of

QUESTION ID:136

Most prominent initial symptom of Vitamin A deficiency in cows and horses is

QUESTION ID:137

Hydropericardium syndrome is otherwise known as

QUESTION ID:138

Scombroid Fish Poisoning is due to the high levels of free ----------- in fish tissue

QUESTION ID:139

The synthetic equivalent of neuropharmacologically active peptides obtained from the marine snail Conus magus is

QUESTION ID:140

Prophenol Oxidase-mediated immune system is found in

QUESTION ID:141

Bryostatin is a marine derived anticancer agent obtained from

QUESTION ID:142

Viral encephalopathy and retinopathy in fishes are caused by

QUESTION ID:143

Environmental gene tags are

QUESTION ID:144

Which one of the following is NOT true of biophotolysis?

QUESTION ID:145

Which is NOT a characteristic of a plasmid used for production of DNA vaccines in fish?

QUESTION ID:146

White spot syndrome virus has

QUESTION ID:147

Haemorrhagic septicemia in carp is caused by

QUESTION ID:148

Primary aetiology of Epizootic Ulcerative Syndrome is

QUESTION ID:149

Net Primary production (NPP) =

QUESTION ID:150

The lower, dense region of the atmosphere is known asA

QUESTION ID:151

The two most important climatic factors affecting the distribution of world biomes are

QUESTION ID:152

A high BOD value in aquatic environment is indicative of

QUESTION ID:153

Benthic organisms of lakes or sea are usually

QUESTION ID:154

Which of these biomes would you expect to find a coyote living in?

QUESTION ID:155

The Ozone layer saves from lethal UV. It mainly absorbs

QUESTION ID:156

The maximum biodiversity in India occurs at

QUESTION ID:157

Extinction rate is high at

QUESTION ID:158

Bootstrapping technique in molecular phylogeny analysis is used to derive additional datasets by

QUESTION ID:159

Which of the following statements regarding scoring matrices of proteins is incorrect?

QUESTION ID:160

Trade off between sensitivity and specificity can be represented by

QUESTION ID:161

Which of the following interactions are NOT characteristics of epitope-paratope interactions?

QUESTION ID:162

While building a model of a protein using homology modeling approach, special care needs to be exercised while assigning conformation of

QUESTION ID:163

Which of the following complementarity determining regions (CDRs) of antibodies is sequentially and conformationally the most variable?

QUESTION ID:164

The LIS technique is used in the MUMmer algorithm for

QUESTION ID:165

The numbers at the internal nodes of a phylogenetic tree indicate

QUESTION ID:166

Which one of the following statements is FALSE?

QUESTION ID:167

Structure determination of myoglobin in 1958 revealed that the protein consists mostly of

QUESTION ID:168

One side of a 30-residue α-helix faces the hydrophobic interior of a protein. If the helical wheel of this helix were to be drawn, which of the following residues are likely to be hydrophobic?

QUESTION ID:169

The dielectric constant of protein interiors is likely to be

QUESTION ID:170

In a helix-turn-helix motif in proteins, which one of tyhe following is true for binding to nucleic acids?

QUESTION ID:171

In the prokaryotic ribosome structure, the peptidyl transferase activity of the ribosomes is found

QUESTION ID:172

Maximum parsimony analysis in the context of molecular phylogeny implies