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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Which of the following is NOT a typical feature of hypersensitive response observed in plants during the attack of invading microbes?

QUESTION ID:2

Plants carrying a transgene for glyphosate resistance will survive a field application of glyphosate that kills weeds. Which of the following enzyme is inhibited by glyphosate?

QUESTION ID:3

A haploid sperm from one species and a haploid egg from another species may form a diploid interspecies hybrid. Meiosis in these plants generally fails but can
lead to rare duplicated gametes called

QUESTION ID:4

Genes essential for embryo organization have been identified by mutant analysis. Which one of the following mutants shows reduction or inhibition of cotyledons and shoot apical meristem?

QUESTION ID:5

Which one of the following is true for the polymertrapping model of sugar diffusion in plant cells?

QUESTION ID:6

The T-DNA of Agrobacterium must be excised from its circular plasmid for its transfer into plant cells. Which one of the following complexes of Vir proteins is responsible for DNA nick at the left and right border sequences?

QUESTION ID:7

What will happen to the growth of a vertically growing root if half of the root cap is removed?

QUESTION ID:8

GA2-oxidase cDNA from bean is overexpressed in wheat plant by genetic engineering. Which one of the following phenotypes correctly describes the resultant transgenic plant?

QUESTION ID:9

Which one of the following statements is NOT correct regarding ethylene signaling in plants?

QUESTION ID:10

Leaf abscission is a phenomenon regulated by the amount of auxin and ethylene. Which one of the following statements is correct regarding the amount of auxin and ethylene during leaf abscission?

QUESTION ID:11

Transgenic plants were developed with a gene conferring krummycin resistance. Four independent Te events were seeted and — 100 T1 seeds were genniutted on kmuunyein. The number of kanamyein sensitive (Flans) and kanamycin resistant (ElanR) progeny obtainer' in each case is listed below: 

Identify the line where the Transgenic has Integrated at a single locus. 

QUESTION ID:12

Using transgenic technology male sterile plants are developed by

QUESTION ID:13

One of the concerns in commercial usage of transgenic plants is the spread of transgene through pollen flow. Which one of the following methods can be used to circumvent this problem?

QUESTION ID:14

Which one of the following can be used as a selection marker for developing transgenic plants?

QUESTION ID:15

Ac transposon is a /an

QUESTION ID:16

SSR is said to be a --------------type of marker

QUESTION ID:17

Direct DNA uptake by protoplasts can be stimulated by

QUESTION ID:18

Aroma in rice is due to

QUESTION ID:19

The male sterile cytoplasm in pearl millet is

QUESTION ID:20

The pathogen against which the crop rotation can be adopted

QUESTION ID:21

Iodine test is used in the detection of

QUESTION ID:22

When urea-denatured inclusion bodies are renatured, protein aggregates can sometimes be found due to misfolding. For small proteins with no disulphide linkages and a single domain, the probability of misfolding is the highest when

QUESTION ID:23

The net charge of a protein may not be sufficient to determine whether a protein will bind to an ion exchanger. This is due to

QUESTION ID:24

In a large scale high cell density fed batch reactor, which one of the following statements is true?

QUESTION ID:25

Optimum bead loading for cell disruption in a bead mill is

QUESTION ID:26

In which one of the following membrane separation processes, concentration difference is the driving force?

QUESTION ID:27

Expanded bed chromatography runs on the principle of

QUESTION ID:28

Which one of the following statements pertaining to ultrafiltration is INCORRECT?

QUESTION ID:29

Which one of the following factors affects the morphology of fungal biomass the least?

QUESTION ID:30

In order to fractionate particles based on size; which of the following centrifuges is best suited?

QUESTION ID:31

Which one of the following purification steps always requires a high initial ionic strength in the sample?

QUESTION ID:32

In Sephadex G series (gel filtration) as the G number increases from 10, 15, 25….200, the pore size of the beads

QUESTION ID:33

In a large scale bioreactor with fungal fermentation, the dissolved oxygen concentration gradient is primarily due to the

QUESTION ID:34

When in a plug flow reactor with low levels of axial mixing, is the maximum product concentration obtained?

QUESTION ID:35

The reasons for existence of lag phase in a batch cultivation process in a bioreactor could be due to the following reasons. Identify the best combination from the following.P: low inoculum size Q:low preinoculum size R:stationary phase inoculum S:inoculum not having the same medium as in batch reactor

QUESTION ID:36

Increasing aeration at a particular rpm does not always lead to increase in oxygen mass transfer in a bioreactor. This is because

QUESTION ID:37

If in a fed batch cultivation of recombinant E. coli, feeding of the substrate is based on the following equation F(t)= [μVX(t)]/SF.YXS. Where μ is specific growth rate and is maintained constant, F(t) is feed rate, X(t) biomass cell concentration,YXS is biomass yield coefficient and SF is the substrate feed concentration. The feed pattern indicates that with increasing time, every cell is receiving the

QUESTION ID:38

Match the KLa determination method in Group I with the characteristics of the method in 1 Group II

P.

oxygen balance method

 1.  

aeration of an actively respiring culture is stopped and switched on again

Q

static gassing out technique

 2.

yields greater OTR and KLa than other methods

R.

dynamic gassing method

 3.

It can be done precisely only at steady state

S.

sodium sulphite oxidation method

 4.

Non respiring biological system is required

QUESTION ID:39

Under unaerated condition in a fermentor, the power consumed by a single impeller was 10,000 W. The diameter of the impeller D is 1m and the density of the medium is 1000 kg/m3 and the stirrer speed is 1s-1. If one more impeller is added to the fermentor, power consumption would be approximately

QUESTION ID:40

For mixing solid contents in a media mixing tank, inclined blade turbine is used because it

QUESTION ID:41

In microbial cultivation experiment with cells having significant maintenance requirements, the measured growth yield Y’ XS (G) and the true growth yield Y XS

QUESTION ID:42

The logistic equation for growth of a bacterial culture is given by 
Where X and Xo are biomass concentration, k and beta are constants. The model depicts that initially when x is small, the growth pattern of the culture would be 

QUESTION ID:43

In an anaerobic fermentor, broth is agitated intensely at 100rpm in a 3.0 m3 baffled tank using a Rushton turbine with diameter of 0.5m. If the diameter of the
impeller is decreased to 0.25m the mixing time in (seconds) would approximately increase ………fold

QUESTION ID:44

Culture comprising a B. subtlis strain by inoculating 1g of cells into 1 liter bioreactor. Fermentor containing 10g/l of glucose. The maximum specific growth rate of the culture is 1.0 /h. The biomass yield from glucose is 0.5 g/g. If the residual glucose concentration left out is 1g/l, the final cell concentration in g/l achieved would be around

QUESTION ID:45

Continuous stirred tank fermenter is characterized by (biomass concentration X kg/m3, residual substrate is S kg/m3, Sf=substrate concentration in feed kg/m3, Dilution rate = D and μm>>D and Km<Sf, XD= the biomass productivity kg/m3h. If the dilution rate is doubled to 2D then the new steady state values in comparison with earlier steady state is characterized by

QUESTION ID:46

Fourier'; law of heat conduction
 
This equation is very similar to 

QUESTION ID:47

A fluidized bed is formed when

QUESTION ID:48

Batch filtration often characterized by continuous increase of the inlet pressure of slurry, is called the ……………filtration

QUESTION ID:49

The most precise method of estimating the metabolic heat produced in an aerobic fermentation process is by

QUESTION ID:50

The ethanol yield per unit mass of substrate consumed (YP/S ) in an anaerobic fermentation process can be improved by

QUESTION ID:51

Exponentially growing cells increase 10 fold in 10 hours, the time required to increase 5 fold will be ………hours

QUESTION ID:52

There are five classes of immunoglobulins [Ig]: IgM, IgG, IgD, IgA and IgE. Upon stimulation of B cells through B cell receptor and a switch factor, which one of the following Ig classes does NOT switch?

QUESTION ID:53

For quantitation of an antigen, researchers A, B, C and D used the following set-up. Which one of the following set up is the most sensitive?

QUESTION ID:54

A mouse deficient in ROR-gammaT will be deficient in

QUESTION ID:55

The specificity in IFN-α/β induced response comes from

QUESTION ID:56

CD152-deficient mice have lymphoproliferative disorder. The disorder is characterized by

QUESTION ID:57

MHC class-II molecule can signal because it

QUESTION ID:58

The T cells in CD28-deficient mice do not proliferate in response to re-exposure to the same antigen. This is because

QUESTION ID:59

CD1 molecules are unique antigen-presenting molecules because they

QUESTION ID:60

Mutations in common gamma chain of receptors for IL-2 cytokine family cause

QUESTION ID:61

Which one of the following conditions generally favours tumour development?

QUESTION ID:62

Which one of the following is NOT a single gene disease?

QUESTION ID:63

Dental plaque is an example of the formation of

QUESTION ID:64

Rheumatic fever is a complication of

QUESTION ID:65

Undulant fever is a characteristic of

QUESTION ID:66

Bubonic plague is the infection of

QUESTION ID:67

Leptospirosis is transmitted by exposure to animal

QUESTION ID:68

Which one of the following is correct when Na ion concentration in the bathing fluid of a squid giant axon is less than that present in sea water, but the osmotic pressure is maintained by substituting it with choline chloride?

QUESTION ID:69

When Tetrodotoxin is administered in bathing medium of a nerve fibre during voltage clamp experiments the membrane current may show which one of the following changes?

QUESTION ID:70

The arrival of the action potential in a presynaptic knob initiates quantal release of neurotransmitter by influx of Ca ions, which may act by

QUESTION ID:71

Which one of the following changes will occur when the external K+ ion concentration in the neuromuscular junction of skeletal muscles is increased?

QUESTION ID:72

The black widow spider venom, alpha-latrotoxin produces its neurotoxicity by

QUESTION ID:73

The generalized seizure is due to the activity of

QUESTION ID:74

Which one of the following genes is related with the late-onset of Alzheimer’s disease?

QUESTION ID:75

The first genetic maps that were developed used genes as markers because

QUESTION ID:76

In E. coil four Hfr strains donate the following genetic markers, shown in the order donated 
All these HFr strains are derived from the same F4 strain. What is the order of these markers on the circular chromosome of the original Ff? 

QUESTION ID:77

Which of the following statements is true?

QUESTION ID:78

In which one of the following evolutionary processes, random changes in allele frequency can lead to a loss of genetic diversity?

QUESTION ID:79

In human pointed eyebrows are dominant to smooth eyebrows and widow’s peak (downward pointed frontal hairline) is dominant to continuous hairline. What phenotypic ratio would you expect in the offspring from a cross between an individual heterozygous for both genes and an individual homozygous recessive for both genes?

QUESTION ID:80

The character regulated by a holandric gene

QUESTION ID:81

Necrosis that develops in tissues subsequent to denaturation of structural and enzymatic proteins soon after death is appropriately referred to as

QUESTION ID:82

The demyelination of the central nervous system white matter produced by the canine distemper virus is an example of

QUESTION ID:83

Catarrhal exudate is observed in

QUESTION ID:84

Sudden onset of heavy mortality in chicken with cyanotic comb and wattles, closed eyes and a semicomatose state are characteristic features of

QUESTION ID:85

Most prominent initial symptom of Vitamin A deficiency in cows and horses is

QUESTION ID:86

Hydropericardium syndrome is otherwise known as

QUESTION ID:87

Scombroid Fish Poisoning is due to the high levels of free ----------- in fish tissue

QUESTION ID:88

The synthetic equivalent of neuropharmacologically active peptides obtained from the marine snail Conus magus is

QUESTION ID:89

Prophenol Oxidase-mediated immune system is found in

QUESTION ID:90

Bryostatin is a marine derived anticancer agent obtained from

QUESTION ID:91

Viral encephalopathy and retinopathy in fishes are caused by

QUESTION ID:92

Environmental gene tags are

QUESTION ID:93

Which one of the following is NOT true of biophotolysis?

QUESTION ID:94

Which is NOT a characteristic of a plasmid used for production of DNA vaccines in fish?

QUESTION ID:95

White spot syndrome virus has

QUESTION ID:96

Haemorrhagic septicemia in carp is caused by

QUESTION ID:97

Primary aetiology of Epizootic Ulcerative Syndrome is

QUESTION ID:98

Net Primary production (NPP) =

QUESTION ID:99

The lower, dense region of the atmosphere is known asA

QUESTION ID:100

The two most important climatic factors affecting the distribution of world biomes are

QUESTION ID:101

A high BOD value in aquatic environment is indicative of

QUESTION ID:102

Benthic organisms of lakes or sea are usually

QUESTION ID:103

Which of these biomes would you expect to find a coyote living in?

QUESTION ID:104

The Ozone layer saves from lethal UV. It mainly absorbs

QUESTION ID:105

The maximum biodiversity in India occurs at

QUESTION ID:106

Extinction rate is high at

QUESTION ID:107

Bootstrapping technique in molecular phylogeny analysis is used to derive additional datasets by

QUESTION ID:108

Which of the following statements regarding scoring matrices of proteins is incorrect?

QUESTION ID:109

Trade off between sensitivity and specificity can be represented by

QUESTION ID:110

Which of the following interactions are NOT characteristics of epitope-paratope interactions?

QUESTION ID:111

While building a model of a protein using homology modeling approach, special care needs to be exercised while assigning conformation of

QUESTION ID:112

Which of the following complementarity determining regions (CDRs) of antibodies is sequentially and conformationally the most variable?

QUESTION ID:113

The LIS technique is used in the MUMmer algorithm for

QUESTION ID:114

The numbers at the internal nodes of a phylogenetic tree indicate

QUESTION ID:115

Which one of the following statements is FALSE?

QUESTION ID:116

Structure determination of myoglobin in 1958 revealed that the protein consists mostly of

QUESTION ID:117

One side of a 30-residue α-helix faces the hydrophobic interior of a protein. If the helical wheel of this helix were to be drawn, which of the following residues are likely to be hydrophobic?

QUESTION ID:118

The dielectric constant of protein interiors is likely to be

QUESTION ID:119

In a helix-turn-helix motif in proteins, which one of tyhe following is true for binding to nucleic acids?

QUESTION ID:120

In the prokaryotic ribosome structure, the peptidyl transferase activity of the ribosomes is found

QUESTION ID:121

Maximum parsimony analysis in the context of molecular phylogeny implies