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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Which one of the following is NOT a typical feature of the hypersensitive response observed in plants during the attack of invading microbes?

QUESTION ID:2

A laboratory is trying to purify a Histone-binding protein from its native source. Which one of the following purification techniques should be used for one-step purification of the protein?

QUESTION ID:3

Given below are two statements one is labelled as Assertion A and the other is labelled as Reason R: 
Assertion A: The dot matrix method of sequence alignment is a very basic method that should be used first, before other more complex methods. Reason R: The diagonal Dot Matrix Display can readily reveal presence of insertions/deletions and direct and inverted repeats that are more difficult to find by the other, more automated methods. 
In the light of the above statements, choose the most appropriate answer from the options given below: 

QUESTION ID:4

Which one of the following is NOT extracted from seaweeds:

QUESTION ID:5

Given below are two statements:
Statement I: Polycistronic mRNAs are found in archaea and bacteria.
Statement II: Plastid genes in eukaryotes have only mono-cistronic mRNA. 
In the light of the above statements, choose the most appropriate answer front the options given below: 

QUESTION ID:6

Differential expression of the genetic material depending on its parentage of inheritance is known as: 

QUESTION ID:7

In an enzyme catalyzed reaction following Michaelis-Menten kinetics, both the enzyme amount and substrate (S) are doubled and we observe that the reaction rate is also doubled. From this, we can conclude that: 

QUESTION ID:8

The ligand binding domain of a ligand gated channel (LGC) has a serine residue, phosphorylation of which is essential for ligand binding. By site directed mutagenesis it was replaced by aspartic acid. The likely consequences will be:

QUESTION ID:9

The animal embryonic stem cells that can differentiate into any embryonic cell type are known as: 

QUESTION ID:10

Skotomorphogenesis is characterized by: 

QUESTION ID:11

A protein sample isolated from natural source was electrophoresed on a non-reducing SDS- PAGE. It migrated as a band corresponding to 60 kDa. When the same protein was electrophoresed on a native-PAGE its migration corresponded to a 120 kDa marker protein. What is a reasonable inference? 

QUESTION ID:12

Continuous Stirred Tank Reactor (CSTR) is running at a dilution (D) of 0.3 h-1 and the product formed is growth associated with Yp/x = 0.2 g/g. In order to obtain productivity = 1.2 gl-lh-1 we need to have at steady state a biomass concentration of: 

QUESTION ID:13

For monoclonal antibody production, mammalian expression systems are preferred over bacterial expression systems, primarily because: 

QUESTION ID:14

Median Distance between the Centroids (MDC),

QUESTION ID:15

What will be the final concentration of the solute when 10 ml of its 10 M solution is mixed with 990 ml solvent? 

QUESTION ID:16

Which one of the following markers is NOT routinely used for DNA fingerprinting

QUESTION ID:17

The CO-NH moiety between two C-alpha atoms constitutes the Peptide bond. The measured bond lengths (Angstroms) are : C(alpha)-CO (1.51); C=0 (1.24); C-N(1.33); N-C(alpha)1.46. In which of these, there is a decrease from the expected bond length due to partial double bond character of the peptide bond? 

QUESTION ID:18

Which one of the following can be considered as an Atomic Vector?

QUESTION ID:19

A protein sequence has twenty consecutive hydrophobic amino acids followed by a few hydrophilic residues and this pattern repeats itself five to seven times. Which kind of a protein is described here? 

QUESTION ID:20

Choose the correct option to fill up the blanks: 
........................... nourish the host coral as well as help it deposit its skeleton. They perform ..................... and pass on some of the.................  they make in the croal

QUESTION ID:21

Which one of the following is NOT a function of the glial cells in the brain? 

QUESTION ID:22

Which one of the following organisms is NOT an obligate intracellular parasite and, therefore. can grow on bacteriological media? 

QUESTION ID:23

The entropy of a growing cell does NOT keep increasing continuously because: 

QUESTION ID:24

Choose the correct statement in respect to metazoan development:

QUESTION ID:25

Which one of the following types of mutations can lead to a major change in the encoded protein?
A. Insertion of a single nucleotide near the end of the coding sequence.
B. Removal of a single nucleotide from the beginning of the coding sequence.
C. Deletion of three consecutive nucleotides of a codon in the middle of the coding sequence.
D. Deletion of four consecutive nucleotides in the middle of the coding sequence.

QUESTION ID:26

Which one of the following DOES NOT represent known interactions of RNA secondary structural elements? 

QUESTION ID:27

If in a bioreactor, the gas hold up (r) is 0.1 and the average diameter of the air bubbles is 1 mm; then the interfacial area (of the air bubble —liquid interface), per unit reactor volume is: 

QUESTION ID:28

Which one of the following is the most accurate statement regarding giardiasis?

QUESTION ID:29

The technique used in animal biotechnology for rapid multiplication and production of animals with a desired genotype is

QUESTION ID:30

Which of the following statement are correct 
A. Lipid bilayer lacking proteins is highly impermeable to all the charged molecules.
B. Channels have specific binding pockets for the solute molecules they allow to pass.
C. Transporters allow solutes to cross a membrane at much slower rates than do channels.
D. The plasma membrane of many animal cells contains open K+ channels, yet the K+ concentration in the cytosol is much higher than outside the cell.
E. The membrane potential of an axon temporarily becomes more negative when an action potential excites it. 

QUESTION ID:31

Yeast cells are immobilized in a column and glucose is fed through the inlet at a concentration of 90 g/l. If all the glucose is consumed and we can treat this as an anaerobic fermentation with negligible cell growth, then the ethanol concentration at the outlet will be: 

QUESTION ID:32

What, if any, is the difference between the molecular weights of molecule A (left) vs molecule B (right)?

QUESTION ID:33

Proteins usually have hydrophobic interiors or cores and hydrophilic exteriors. If a protein comprises hydrophobic patches on its outer surface, what can it mean? 

QUESTION ID:34

Ground shark cartilage ointment is popular for its role in fast wound recovery. The active ingredient primarily responsible for this function is: 

QUESTION ID:35

A following reaction is occurring in a test tube: 
A+B⇌C 
100 nM solution of A is incubated with an equal volume of 100 nM solution of B and left at 25°C till the equilibrium is reached. Finally, when the concentration of A is measured, it is found to be 10 nM. What is the approximate concentration of C when 2 nM solution of A is incubated with an equal volume of 100 nM solution of B under identical conditions? 
Choose the correct answer: 

QUESTION ID:36

A concentrated feed of substrate is added at a constant rate in a fed-batch reactor. If all the substrate is utilized, the specific growth rate (μ) pattern will be: 

QUESTION ID:37

Which one of the following properties of naturally occurring D-amino acids is INCORRECT: 

QUESTION ID:38

A spectrum that depicts the magnitude of response of a biological system to light as a function of wavelength is called: 

QUESTION ID:39

In animal cloning, donor somatic cells should be in which stage of cell cycle? 

QUESTION ID:40

A diafiltration process is used to remove salts from a protein solution of 1L. After 1L of make-up buffer (without salts) has been added to this system, the residual salt concentration would be of the original.

QUESTION ID:41

What will be the absorbance of a 1 mM solution of molecule X whose molar extinction coefficient is 1000 M'tcm"1A path length of the cuvette used is I cm? 

QUESTION ID:42

During analysis of Next-Gen Sequencing data, what is the advantage of BAM fan over SAM files? 

QUESTION ID:43

Amide bonds between two amino acids (as shown below) are general Is in trans- confrontation. 1 he Muni. are labeled as A1 through" A6. the angle measured for defining the trans conformation of the amide bond is the angle between

QUESTION ID:44

Which command is suitable to list out all the directories in the working directory, in linux/unix environment? 

QUESTION ID:45

Which one of the immunodeficiency is caused by a mutation in BTK gene:

QUESTION ID:46

In animal cloning protocol, while performing enucleation, which chemical is used for relaxing the cellular cytoskeleton? 

QUESTION ID:47

A 1 M Tris-HCI (pH 8.0) solution was diluted 10 fold. What will be the pH of the resulting solution? 

QUESTION ID:48

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Mycoplasma is a bacteria without cell wall, and it stains negative in Gram staining.
Reason R: Only bacteria that have a cell wall would stain positive in Gram staining. In the light of the above statements,
choose the correct answer from the options given below: 

QUESTION ID:49

The most likely sub-cellular localization of NAC transcription factors is: 

QUESTION ID:50

Nod gene products: 

QUESTION ID:51

In plants, the overexpression of lectin genes confers resistance to: 

QUESTION ID:52

Given the molecular structure of glucose, the molecular formula and molecular weight of maltose will be:

QUESTION ID:53

DNA isolated from four unidentified species of bacteria — A, B, C and D has been estimated to have 38%, 26%, 24% and 12% thymine, respectively. One of these four species was isolated from a hot spring (sec). Identify the candidate which is most likely to be a thermophilic bacterium. 

QUESTION ID:54

VirA and VirG form a two component signaling system during Agrobacterium infection in plants. In an experiment, VirG was mutated resulting in nonfunctional VirG protein. Which one of the following consequences is most likely? 

QUESTION ID:55

Which one of the following is comparatively the best E-value to a palmist sequence match? 

QUESTION ID:56

A haploid sperm from one species and a haploid egg from another species may form a diploid interspecies hybrid. Meiosis in these plant, generally fails but can also lead to rare duplicated gametes called: 

QUESTION ID:57

Arrange these soluble cations (Na, Ca, K, Mg) in ascending order of abundance of soluble cations found in saline water bodies: 

QUESTION ID:58

The red color of beetroot is due to the presence of:

QUESTION ID:59

Which family of proteins has the greatest likelihood of containing a Leucine-zipper motif? 

QUESTION ID:60

Which enzyme will digest RNA In a DNA-RNA Hybrid? 

QUESTION ID:61

Which one of the following is NOT applicable for a chemical synapse? 

QUESTION ID:62

When ice absorbs latent heat at a constant pressure to melt from solid to liquid state at constant temperature, which of the following changes are happening? 
A. Entropy of the system is increasing.
B. Cp (heat capacity at constant pressure) of the system is infinite or undefined.
C. Enthalpy of the system is increasing.
D. Cp of the system is zero. 
Choose the correct answer: 

QUESTION ID:63

Which one of the molecular processes utilize 2', 3' Dideoxy-ribonucleoside triphosphates? 

QUESTION ID:64

given below are two statements: 
Statement I: RNA secondary structure is composed primarily of double-stranded RNA regions formed by folding of the single-stranded molecule back on itself.
Statement II: Like protein secondary structure, RNA secondary structure can be conveniently viewed as an intermediate step in the formation of a three-dimensional structure. 
In the light of the above statements, choose the most appropriate answer from the options given below 

QUESTION ID:65

Which one of the following method can be used to treat crude sewage and nitrify secondary effluent? 

QUESTION ID:66

Which one of the following statements is most relevant to leishmaniasis? 

QUESTION ID:67

How many different kinds of post translational modifications are present in this peptide? 


QUESTION ID:68

Which one of the following is NOT a Data Type?

QUESTION ID:69

Given below are two statements one is labelled as Assertion A and the other is labelled as Reason R: 
Assertion A : In size exclusion chromatography, larger proteins elute earlier and smaller proteins elute later. Reason It : When matrices like sephadex are used, the partitioning of protein molecules between liquid and solid phases is a function of protein size 
In the light of the above statements, choose the correct answer from the options given below: 

QUESTION ID:70

Which one of these is a metal containing Vitamin? 

QUESTION ID:71

Proteins are glycosylated in the endoplasmic reticulum. In the final structures, the glycan moieties are typically present: 

QUESTION ID:72

Short Chain variable fragments (ScFv) of antibody are composed of : 

QUESTION ID:73

A conventional light microscope allows us to magnify cells up to 1000 times and to resolve details as small as 

QUESTION ID:74

Arrange the following chemical bonds/interactions in the order of their strengths in vacuum - Hydrogen bond, Covalent bond, van der Waals attraction and Ionic bond: 

QUESTION ID:75

Identify the correct statement about Fucoidans: 

QUESTION ID:76

Green Fluorescent Protein (GFP) emits green light due to the presence of a: 

QUESTION ID:77

Which one of the following genes can be used to generate male sterile crop plants? 

QUESTION ID:78

which one of the following lines of unix commands can tell you the number of lines in a file? 

QUESTION ID:79

Given below are two statements one is labelled as Assertion A and the other is labelled as Reason R: 
Assertion A: Phosphomannose isomerase (PMI) from E. coil was used as selection marker to develop 'golden rice 2'.
Reason R: When mannose is added in the selection media, endogenous hexokinase converts mannose into mannose-6-phosphate which blocks glycolysis, ATP production, and represses transcription of photosynthetic genes, resulting in slower growth of non-transformed plants. PMI, on the other hand, converts mannose-6-phosphate to fructose-6-phosphate. 
In the light of the above statements, choose the correct answer from the options given below: 

QUESTION ID:80

T- cell clones in an individual recognize peptides only when bound and displayed by that individual's MHC molecules on the surface of antigen presenting cells. This process Is known as: 

QUESTION ID:81

Where is the greatest likelihood of the occurrence of a Cysteine residue in a protein structure? 

QUESTION ID:82

If the respiratory quotient (R.Q.) for growing cells is 1.1 and the exit gas analyzer of a bioreactor shows 3% CO2 in the outlet gases; then given normal air as input to the reactor, you expect the outlet 02 concentration to read approximately: 

QUESTION ID:83

miRNA  are major regulators of gene expression. In an experiment, it was observed that gene 'X' is a direct target of miRNA 'A'. Upon overexpression of miRNA 'A' in Arabidopsis thaliana: 

QUESTION ID:84

In a plant transformation vector, the selectable marker gene is ideally located next to the "left border" of T-DNA region, because: 
A. Left border of T-DNA enters last into the plant cell.
B. Right border of T-DNA enters last into the plant cell.
C. It ensures complete transfer of T-DNA into the plant cell. 
Choose the most appropriate answer from the options given below:

QUESTION ID:85

What will be the molecular weight of the tri-peptide Molecular weights of Trp, Gly, and His are 204.2, 75.07, and 155.2 respectively. 

QUESTION ID:86

The principle microorganism for yogurt is: 

QUESTION ID:87

A continuous reactor has a productivity of 0.5 g/l/h. The volume of reactor or required to produce 1.2 ton/day of the product is

QUESTION ID:88

Match items, in List I with items in List II.  

List1

List2

A. Down Syndrome

I. Trisomy 18

B. Edward Syndrome

II. XXY

C. Klinefelter Syndrome

III. Female with one X

D. Turner Syndrome

IV. XX+21

Choose the correct answer from the options given below 

QUESTION ID:89

Given below are two statements: 
Statement I: MacConkey agar is a media used for recovery of Gram positive bacteria from mixed bacterial culture.
Statement II: The bile salts and crystal violet in MacConkey agar inhibit growth of some bacteria. 
In the light of the above statements, choose the correct answer (sum the options given below : 

QUESTION ID:90

One centimorgan is defined as the genetic distance between two loci with a statistically corrected recombination frequency of: 

QUESTION ID:91

A bacterial signaling system that regulates population density-dependent gene expression using secreted chemical signals is known as

QUESTION ID:92

Which one of the following CANNOT be used for progressive global multiple sequence alignment

QUESTION ID:93

In a batch culture of cells following Monad Kinetics, the declining log phase lasts longer when:

QUESTION ID:94

Which regions of the genome are best suited for generating DNA Barcodes for species identification?

QUESTION ID:95

The color of emitted light of Quantum dots used in fluorescence microscopy depend on:

QUESTION ID:96

Cryptochromes control photomorphogenesis in response to: 

QUESTION ID:97

Yeast cells obtained from batch fermentation can be reutilized for the production of: 

QUESTION ID:98

Commercial production of citric add is done by fermentation using: 

QUESTION ID:99

Which of the following are the sulfur containing amino acids?
i. Methionine
ii. histidine
iii .Leucine
iv. Cysteine 

QUESTION ID:100

In a metabolic engineering experiment the flux from primary carbon metabolism was diverted towards methylerythritol phosphate (MEP) pathway. Which one of the following class of secondary carbon compounds do you think will accumulate more? 

QUESTION ID:101

Which one of the following is NOT a food rich in Vitamin A?

QUESTION ID:102

Which form of the plasmodia is transmitted from mosquito to human? 

QUESTION ID:103

Due to the difficulty of isolating and culturing, many marine microorganisms are regarded as "viable but non-culturable" (VBNC). Which one of the following is a suitable method to hunt for useful genes in these organisms? 

QUESTION ID:104

Which one of the following process is NOT used to convert the hydrocarbons present in the organic compounds to central metabolic intermediates? 

QUESTION ID:105

Which one of the following is true for PAM250 Scoring Matrix? 

QUESTION ID:106

Given below are two statements: 
Statement I: Fungi are important degraders of polymers and are used in the composting and biodegradation of toxic organic substances. Statement II: Fungi are able to selectively excrete toxins present in these polymers. 
In the light of the above statements, choose the correct answer from the options given below: 

QUESTION ID:107

You wish to reduce the maximum shear rate in your reactor (given by impeller tip speed), without changing the power consumption (agitator power). For this, you will:

QUESTION ID:108

A protein completely unfolds in 8 M Urea. The stability of the folded state in water is calculated to be 8 kCal/mole at 25°C. What is the likely AG of the following reaction in 8 M Urea? A(native) →A(unfolded) 
Choose the correct answer:

QUESTION ID:109

Given below are two statements: 
Statement I: An alignment is generated by starting at the ends of the two sequences and attempting to match all possible pairs of characters between the sequences and by following a scoring scheme for matches, mismatches, and gaps. Statement II: For proteins, an amino acid substitution matrix, such as the Dalhoff percent accepted mutation matrix 250 (PAM250) or Blossom substitution matrix 62 (BLOSUM62) is used to score matches and mismatches. 
In the light of the above statements, choose the most appropriate answer from the options given below: 

QUESTION ID:110

Match the neon in left column with those in the right column 

Conjugated protein

Prosthetic group

A. Casein

I. Calcium

B. Hemoglobin

II. Tron

C. Calmodulin

III. Flavin nucleotides

D. Succinate dehydrogenase

IV. Heme

E. Ferritin

V. Phosphate group

Choose the correct answer Irons the options given below: 

QUESTION ID:111

Witt is the molarity of 100 g Glycine (75 Daltons) dissolved in 1 liter water? 

QUESTION ID:112

Which one among the following is the correct function of Iegharnoglobin?

QUESTION ID:113

RTS,S vaccine I or malaria consists of the central repeat (11) region deiseal forms plasmodium falciparum cirummorozoite protein (CSP) that is genitalless Insect to the T- cell epitope (3) and surface antigen (S) regions derides Irons: 

QUESTION ID:114

Where is the greatest likelihood of the occurrence at a Proline residue it a protein structure? 

QUESTION ID:115

15 to 30% of collagen residues an- 4 hydroxyproline residue (HPy). When rats ate led with 14C labeled Hyp the ...collagen produced by them is not radioactive. but when they are led with 14C labeled Praline. the collagen produced is radioactive. What is the most plausible reason?

QUESTION ID:116

An exponentially increasing leaf of concentrated .substate is led Soo a led batch reactor to maintain a courant specific growth rate 0.35 H-1. if  the feed is 5ml/hr at time. t 0. then feed alter 4 hrs will be approximately

QUESTION ID:117

Which one of the following enzymes help the DNA to overtone the tension generated by the  unwinding during replication? 

QUESTION ID:118

In a neuronal culture experiment. the effect Of a chemical (under study) was prevented by pretreating the cells with prazosin. an alpha) adenomere antagonist but not In propranolol. a beta adrenoceptor antagonist. The chemical under studs is likely to be: 

QUESTION ID:119

Latency is a characteristic of which one of the following viruses? 

QUESTION ID:120

How many chiral centers are there in each of these two molecules ? 

QUESTION ID:121

In an external rec)de reactor (CSTR with external recycle), the outlet stream b concentrated 20 fold with respect to biome. and half of this concentrated cells is fed back into the reactor. In such a situation, washout will take place w hen dilution rate (D)

QUESTION ID:122

Which one of the following cell separation techniques allows cells (tom different stages of the cell rule to be separated simultaneously with. minimal perturbation to their physiology ? 

QUESTION ID:123

Protein A used for the purification of IgG is isolated from: 

QUESTION ID:124

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R Assertion A: Heating of preoptic anterior hypothalamic area of the brain caused profuse sweating and dilation of blood vessels of the skin. Reason It The above (in A) is to absorb more heat into the body and throw out waste from the body. In the light of the above statements, choose the correct answer from the options given below: 

QUESTION ID:125

The energy landscape or the energy of a reaction:
                  A <---> B 
Along its reaction coordinate follows the following pattern: 

Which one of the following statements are true?

QUESTION ID:126

If an enzyme is NOT damaged during the process of entrapment in a bead, then internal pore diffusion will: 

QUESTION ID:127

Your friend runs a BLASTp on his unknown sequence and finds a match with E-value of 3 with a target. What does this mean?

QUESTION ID:128

Which one of the following is NOT a major checkpoint in the cell division cycle of eukaryotic cell? 

QUESTION ID:129

In a water sample collected from an industrial duster, the ratio of BOD:COD is found to be less than 0.3. Which one of the following may be presumed from this initial data? 

QUESTION ID:130

For determining V, and Km of an enzyme catalyzed reaction, we often use a double reciprocal plot of 1/v versus 1/s. This is NOT the best method because: