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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Two sides of an isosceles triangle measure 3 cm and 7 cm. Which one of the following is the measure (cm) of the third side?

QUESTION ID:2

At 9 AM, the shadow of a 5' (feet) tall boy is 8'. At the same time, shadow of a flagpole beside is 28 feet. What is height of the flagpole?

QUESTION ID:3

What number should appear next in this series: 8, 12, 10, 14, 12, 16….?

QUESTION ID:4

Two numbers are more than a third number by 20 % and 50 %, respectively. The ratio of the first two numbers is

QUESTION ID:5

Identify from the options given below the unwritten number (?) in the series: 360, ?,
180, 60, 15, 3

QUESTION ID:6

For the chemical reaction C2H5OH(ℓ) + 3O2 (g) → 2CO2 + 3H2O (ℓ), if change in internal energy at 298K is 1368 KJ/mole, the change in enthalpy will be

QUESTION ID:7

If a drop of sweat evaporates from body, the work done is

QUESTION ID:8

Dissociation of an aqueous acid (HA) gives HA + H2O H3O+ + A-, then pKa is

QUESTION ID:9

The sides of a rectangle are in the ratio of 4:3 and its area is 108 cm2. The perimeter of the rectangle in cm is

QUESTION ID:10

If each side of a cube is increased by 1%, the percentage change in the volume would be approximately

QUESTION ID:11

A brick weighs ¾ of itself and ¾ of a Kg. The weight of the brick in Kg is

QUESTION ID:12

In an enzyme assay, the corrected absorbance reading obtained on addition of 100μl of the enzyme extract is 0.025. Given that one unit of the enzyme is the amount of enzyme required to increase the absorbance by 0.001 units under assay conditions, the enzyme activity (units/ml) of the extract is:

QUESTION ID:13

The cell-free extract prepared from E. coli cells over-expressing enzyme β- glucosidase showed the activity of 1.5 units per ml (protein concentration 2 mg per ml). The Ni-NTA purified preparation showed the activity of 75 units per ml (protein concentration 100 μg per ml). Calculate the fold purification of the enzyme
achieved?

QUESTION ID:14

Enzyme X showed its activity on substrate A (375 units per ml), substrate B (185 units per ml) and substrate C (75 units per ml). With respect to substrate A, the
percent activities on substrate B and C are --------, respectively.

QUESTION ID:15

Protein ‘A’ from Pseudomonas putida contains 135 amino acids. The number of nucleotides present in the gene encoding the protein will be -----.

QUESTION ID:16

The molarity of a 15 % of NaCl solution in water is ----.

QUESTION ID:17

The reaction velocity, (V) vs substrate concentration [S] profile was performed for Enzyme A using 1 μg enzyme per assay. Similar experiment was carried out under identical conditions except that the concentration of enzyme used was 2 μg per assay. Under these conditions, the kinetic constants

QUESTION ID:18

Which one of the following bacterial cell suspension at A540nm = 0.2 will have the maximum number of cells per ml?

QUESTION ID:19

Two solutions of a substance (non-electrolyte) are mixed in the following manner: 480 ml of 1.5 M first solution + 520 ml of 1.2 M second solution. What is the molarity
(M) of the final mixture?

QUESTION ID:20

A 29 % H2SO4 solution having a molarity of 3.60, would have a density (g.ml–1) of --. (MW of H2SO4 is 98)

QUESTION ID:21

Calculate the turnover number (sec-1) for an enzyme, if Km = 0.001 μM; Vmax = 10 μM/sec; and E0= 0.001 μM.

QUESTION ID:22

Zymomonas mobilis cells are grown in a chemostat culture in a 60 litre (l) fermenter. The Ks and μmax for the organism is 0.2 g.l- 1 and 0.2 h-l, respectively. What flow rate (l.h-1) is required for a steady-state substrate concentration of 0.2 g.l-1?

QUESTION ID:23

Consider three independently assorting gene pairs, A/a, B/b, and C/c. The probability of obtaining an offspring of AABbCc from parents that are AaBbCC and AABbCc is

QUESTION ID:24

Given that husband and wife are both heterozygous for a recessive allele for albinism. If they have dizygotic twins, the probability that both the twins will have the
same phenotype for pigmentation will be

QUESTION ID:25

In a family with three children, what is the probability that two are boys and one is a girl?         

QUESTION ID:26

LDL binds with cell surface receptor and gets internalized via clathrinmediated endocytosis. This process helps in maintaining the cholesterol-LDL level in the plasma. However, in a disease known as familial hypercholesterolemia (FH), very high levels of plasma cholesterol is found. This could be due to mutation in which one of the following genes in FH patients?

QUESTION ID:27

Digestion of a 5Kb linear DNA fragment with EcoRI generates two fragments of 2 Kb and 3 Kb, while digestion of the same molecule with HindIII yields three fragments of 0.7 Kb, 3.5 Kb and 0.8 Kb. When the same DNA is digested with both the enzymes, it yields fragments of 0.7 Kb, 1.3 Kb, 2.2 Kb and 0.8 Kb. The right sequence of restriction sites in the DNA fragment is

QUESTION ID:28

GFP, when overexpressed in a cell, remains mostly in the cytosol. A GFP construct is modified such that the resultant GFP protein will have a conjugated peptide Pro-Lys-Lys-Lys-Arg-Lys-Val at its N-terminus. If such a GFP construct is expressed in a cell, the modified GFP protein will be localized in the

QUESTION ID:29

Succinate dehydrogenase converts succinate to fumarate. Which one of the following is TRUE when the competitive inhibitor malonate is added in the reaction mixture?

QUESTION ID:30

In a mammalian cell, protein synthesis is regulated at the level of initiation by various kinases. During viral infection, which one of the following kinases is involved in regulating the step of formation of eIF2.GTP.Met tRNAi ternary complex in the host?

QUESTION ID:31

Which one of the following side chains of an amino acid is responsible for fluorescence in proteins?

QUESTION ID:32

DNA molecules labeled with 15N and 14N can be separated by

QUESTION ID:33

Match the chromatographic technique from Group A with the appropriate

QUESTION ID:34

Cytoskeletal organization of a cell is regulated by

QUESTION ID:35

In comparison to animals residing in a warm climate, animals living in cold climate need thermal insulation. The cell membranes of the latter would have a relatively higher content of ……

QUESTION ID:36

In glycolysis, the conversion of 1 mol of fructose 1,6-biphosphate to 2 mol of pyruvate results in the formation of

QUESTION ID:37

In diabetic ketoacidosis, increase in which of the following would cause elevated production of ketone bodies?

QUESTION ID:38

Which one of the following modes of inheritance is seen in Cystic Fibrosis?

QUESTION ID:39

Statins are very effective against hypercholesterolemia, a major cause of atherosclerosis. These drugs reduce plasma cholesterol levels by

QUESTION ID:40

Measles, Mumps, Rubella-MMR combined vaccine represents which one of following vaccine categories?

QUESTION ID:41

A haemophiliac man marries a normal woman. They have a daughter who does not show symptoms of haemophilia. If she marries a haemophiliac man, what will be the probability of their son displaying symptoms of haemophilia?

QUESTION ID:42

The conventional treatment for methanol toxicity is to administer ethanol. Which of the following explains the basis of this treatment?

QUESTION ID:43

What will be the angular velocity of a rotor in a centrifuge operating at 6000 revolution per minute?

QUESTION ID:44

Lysosomes of a cell were labelled with lysotracker Red. Subsequently, these cells were infected with GFP-transfected Mycobacterium and observed under a fluorescence microscope. What will you observe?

QUESTION ID:45

A linear DNA fragment which has 3 restriction sites for BamH1, is labeled only at the 5/ end. This DNA is partially digested with BamH1 in such a way that all kinds of fragments are generated. Under these conditions, how many labeled and unlabeled fragments will be produced?

QUESTION ID:46

In the preparation of humanized antibody, part of the antibody molecule is taken from mouse and the remaining is taken from that of human, through genetic engineering technique. Which one of the following statements is true for humanized antibody?

QUESTION ID:47

Approximately how many molecules of CO2 are produced daily by oxidative metabolism in adult human? Avagadro’s number is 6.02 X 1023.

QUESTION ID:48

A 25-year old man undertakes a prolonged fast for religious reasons. Which one of the following metabolites will be elevated in his blood plasma after 24 hours?

QUESTION ID:49

Which one of the following is not a deficiency disorder?

QUESTION ID:50

Electrophoresis of a purified protein in SDS-PAGE in the presence of 2- mercaptoethanol yields two bands of 35 kDa and 45 kDa. However, in a gel filtration chromatography, the same protein elutes as 80 kDa. What conclusion will you draw from the results?

QUESTION ID:51

Which one of the following virus is the odd one out?

QUESTION ID:52

DAPI 4',6-diamidino-2-phenylindole is a fluorescent stain used for staining of:

QUESTION ID:53

During antigen presentation by antigen presenting cells, viral antigens are processed and presented to T cells in the context of MHC antigens. Viral antigens are processed

QUESTION ID:54

Which of the following is not specifically protected by intellectual property legislation?

QUESTION ID:55

Teichoic acid possibly plays a role in growth of bacterial cell by regulating the activity of

QUESTION ID:56

The fungus that causes athlete’s foot is

QUESTION ID:57

The following amino acid is

QUESTION ID:58

A DNA fragment was cloned at the EcoRI restriction site of a plasmid vector that contains antibiotic resistance genes, kanR and specR. It was observed that all the positive clones (containing the DNA fragment of interest) grew on medium with kanamycin but not on media containing spectinomycin. In the absence of any other confounding factors, which one of the following statements would explain this observation?

QUESTION ID:59

A cDNA encoding an eukaryotic gene was ligated to an expression vector which was then introduced into E. coli for expression of the protein. The experiment resulted in poor expression of the protein. However, expression improved significantly in a Rosetta strain of E. coli which demonstrate that the initial poor level expression was due to

QUESTION ID:60

In Drosophila embryogenesis, the signal received from Gurken proteins by follicle cells results in posteriorization of these cells. Knocking out the gene for Gurken in Drosophila will NOT result in failure of

QUESTION ID:61

A patient suffering from an infectious disease had high levels of TNF alpha and IL-6. Assuming there are no other interactions, which T cell population is likely to dominate in his blood sample?

QUESTION ID:62

The niche of stem cells in human skin is the

QUESTION ID:63

In persons suffering from sickle cell anemia, the 6th amino acid of beta globin protein is valine instead of glutamic acid. This has occurred due to substitution mutation leading to a change in glutamic acid codon to valine codon in the gene. The mutation is a

QUESTION ID:64

A covalently closed circular DNA was in relaxed state in water at 300C. What will happen to the supercoiling state of the molecule, if water temp increases to 600C or decreases to 100C?

QUESTION ID:65

1000 color blind males underwent random mating with 1000 normal females (neither carrier nor color blind). After how many generations, the allele frequency in both the sexes in the population will reach equilibrium?

QUESTION ID:66

Acclimatization to high altitude in humans induces differentiation of

QUESTION ID:67

The protection against smallpox afforded by prior infection with cowpox presents

QUESTION ID:68

What is glycosuria?

QUESTION ID:69

The mechanism that permits immunoglobulins to be synthesized in either a membrane bound or secreted form is

QUESTION ID:70

Which of the following is NOT involved in antigen- antibody complexes?

QUESTION ID:71

Iron uptake by the cells from extracellular environment is mediated through

QUESTION ID:72

Which of following viruses causes liver cancer?

QUESTION ID:73

Which one of the following enzymes is an established intracellular antioxidant?

QUESTION ID:74

A given cytokine has different biological effects on different target cells. This is known as

QUESTION ID:75

Influenza virus contains 11 proteins, of which the two major glycoproteins are H and N. What do H and N stand for in influenza strains nomenclature?

QUESTION ID:76

Inosine in the tRNA anticodon will base pair with all except which one of the following bases in the codon of mRNA?

QUESTION ID:77

In Electrophoresis if the buffer pH is above the isoelectric point of the protein, the protein will

QUESTION ID:78

In the ABO blood group system in humans, alleles IA and IB are codominant and both are dominant to the allele i. If a type B with genotype (IB i ) woman marries a type A with genotype (IA i ). The probable children to the couple would be of the type

QUESTION ID:79

Circulating testosterone is mostly present in bound form, which of the following proteins binds weakly to the major amount of circulating testosterone?

QUESTION ID:80

T. brucei complex transmitted to man by byte of several species of tsetse fly can cause

QUESTION ID:81

In immune responses at mucosal surfaces, particularly in the context of gut immunology, which of the following is NOT true?

QUESTION ID:82

You have developed a H-2 k/d mouse model with grafted thymus for studying T cell maturation. The phenotype of the grafted thymus is H-2d/d. You have immunized the mouse with antigens derived from Listeria monocytogenes and would like to assess the activation of cytotoxic T cells. Which among the following would be your choice of target cell?

QUESTION ID:83

While analyzing protein sequences of rpoB gene among different bacteria, different changes in amino acid sequences were observed among the homologs due to base substitution. If transition is more frequent than transversion, single mutation is more frequent than double mutation, which one of the following changes will occur most frequently than others?

QUESTION ID:84

If G2/M check point and DNA repair proteins are working optimally in a cell and there are some DNA damages during G2 phase, which one of the following would be the response of the cells to damage?

QUESTION ID:85

Transferrin binds with its receptor and recruits clathrin via which one of the following proteins?

QUESTION ID:86

Which one of the following is the major product of fatty acid synthase?

QUESTION ID:87

Intracellular transport in mammalian cells through vesicular fusion is regulated by which among the following GTPases?

QUESTION ID:88

Which one of the following processes is NOT regulated by the kidney?

QUESTION ID:89

Rapid removal of large quantities of blood will cause an animal to go into hypovolemic shock and may even cause death. If the weight of a mouse is 20 gms, what is the amount of maximum blood one can withdraw from a single bleed to prevent the mouse from going into hypovolemic shock?

QUESTION ID:90

In order to have a desired shRNA cassette integrated in target cells, which of the following gene transfer vectors is preferable?

QUESTION ID:91

Which one of the following statements regarding naturally occurring Agrobacterium strains is true?

QUESTION ID:92

The integration of T-DNA in the plant nuclear genome is most likely due to

QUESTION ID:93

Variation in transgene expression levels among independent transgenic events generated using the same transgene construct could be due to
(i) position effects
(ii) strength of the promoter used to express the transgene
(iii) variation in copy number of the transgene
(iv) presence of restriction enzyme sites in the transgene sequence

QUESTION ID:94

A typical flowering plant has a combination of sporophytic (diploid) and gametophytic (haploid) tissues. Which of the following types of plant tissues do NOT contribute to the formation of a mature fruit?

QUESTION ID:95

The submerged leaves of an aquatic plant are feathery (to avoid damage due to flowing underwater currents) while leaves on the surface are padded (to help in floatation). The correct statement related to the above phenotype is

QUESTION ID:96

Which one of the following statements related to the development of insect-resistant transgenic plants is correct?

QUESTION ID:97

When the gene and the promoter used for modification of a plant using transformation technology are derived from sexually compatible species, the modified plant thus generated is known as a

QUESTION ID:98

A transgenic plant segregates in a 3:1 ratio for the transgenic: nontransgenic phenotype in T1 progeny derived by self-pollination. This indicates that

QUESTION ID:99

Which one of the following reporter genes can be used for real-time visualization of living cells/tissues in transgenic plants?

QUESTION ID:100

Which of the following statements represent correct features of sexual and asexual modes of reproduction in flowering plants?
(i) In sexual reproduction, progeny are genetically different from each other.
(ii) In asexual mode of reproduction, progeny are genetically identical to each other but different from the parent.
(iii) Development of greater adaptive ability is possible only in case of progeny derived by sexual reproduction.
(iv) A minor change in the habitat may adversely affect all offspring derived by asexual reproduction.

QUESTION ID:101

Which one of the options given below represents the correct combination of plant defense responses listed in Column A and Column B?

QUESTION ID:102

Non-conditional, negative selection marker genes are usually expressed under a

QUESTION ID:103

A method in which a strong enhancer is randomly inserted in a plant genome by transformation, resulting in mutant plants with dominant phenotypes, is known as

QUESTION ID:104

Which one of the following organisms is used by plant biologists to study translocation in phloem?

QUESTION ID:105

In a genetic transformation experiment, a researcher failed to add the antibiotic selection agent in the shoot regeneration medium for selection of transgenic plants. In the absence of any other confounding factors, which one of the following statements is expected to be correct?

QUESTION ID:106

Orientation of a cloned passenger gene cassette in a binary plasmid vector, containing the expression cassette of a selectable marker gene, can be checked by PCR using

QUESTION ID:107

Somatic hybrid plants with full or nearly full complement of the two parental species are called

QUESTION ID:108

In CAGE,

QUESTION ID:109

Which one of the following statements about associative nitrogen fixing bacteria is NOT true?

QUESTION ID:110

According to the Seeds Act of 1966, which one of the following functions is NOT applicable to a Seed Certification Agency?

QUESTION ID:111

Which one of the combinations given below represents populations with a similar genetic composition?

QUESTION ID:112

Which one of the following statements represents a correct definition of Gene Pyramiding?

QUESTION ID:113

A marker closely linked to the gene of interest for an agronomic trait will be used for

QUESTION ID:114

The vitrification of cultured explants is generally caused by

QUESTION ID:115

The Rht mutations in wheat that were pivotal for 'Green Revolution' reduce plant height due to impairment in

QUESTION ID:116

Steady state for a closed system implies

QUESTION ID:117

In a lab scale reactor, the size of the air bubbles in the reactor is primarily determined by

QUESTION ID:118

Generally fungal fermentations are pseudoplastic. Hence in large reactors the cells

QUESTION ID:119

Mixing time increases with the volume of the reactor because of increase in the

QUESTION ID:120

The Reynolds number is a ratio of

QUESTION ID:121

The equation τ = μ du/dy is also termed as

QUESTION ID:122

Match the Transport process with appropriate time constants given below.

Where V is volume; Q is pumping rate; N is agitation rate; D is diameter of the impeller; A is area and ρ is the density of the liquid

QUESTION ID:123

When a liquid of density 1200 kg/m3 and viscosity 0.01 kg m-1s-1 flows through a 1 cm ID pipe at 0.2 m/s, the flow is referred as a

QUESTION ID:124

In a dynamic gassing out technique for determination of KLa in a bioreactor, upon restart of the aeration the increase in DO slows down with time because

QUESTION ID:125

Under unaerated conditions in a fermenter, the power consumed by a single impeller is 10 KW and upon changing the agitation rate from 200 to 600 rpm, the new power consumption (KW) would be -----. (Assuming that the power number remains constant)

QUESTION ID:126

Fill in the blanks with appropriate option: Vinegar is a liquid consisting of acetic acid produced by the fermentation of _______________ by _____________________.

QUESTION ID:127

A food stored in a refrigerator has caused an outbreak of food borne illness. Which one of the following is most likely to be the pathogen responsible for this?

QUESTION ID:128

To reduce the tartness in wine due to malic acid, a secondary fermentation is carried out using

QUESTION ID:129

Which one of the following statements is INCORRECT with respect to food rheology?

QUESTION ID:130

Which one of the following enzyme(s) would result in high glucose yield from starch hydrolysis?

QUESTION ID:131

The major metabolite formed and the succession of microorganisms responsible for Sauerkraut fermentation are

QUESTION ID:132

Omega 3 fats are good for health and play an important role in prevention of heart disease. If you have to formulate a food product rich in omega 3 fats which one of the following options would you choose?

QUESTION ID:133

For a well-established enzyme assay method where the reaction kinetics should remain zero order, what percentage of substrate is converted to product during the incubation period?

QUESTION ID:134

As the viscosity increases, the method used for mixing is changed in the following order

QUESTION ID:135

On scale up, the effective area available for heat transfer in a bioreactor

QUESTION ID:136

A fermenter produces 100 kg lysine per day. If the volumetric productivity is 0.8 gl-1h-1, what is the volume of the fermenter in liter?

QUESTION ID:137

Chemostat cultivation is carried out at steady state with a dilution rate of 0.1 h-1 . Given μm = 1.1 h-1and Ks=0.1 g/L. What will be steady state substrate concentration?

QUESTION ID:138

For degradation of crude oil in ocean by microorganisms, the two limiting nutrients are

QUESTION ID:139

The first circumpolar expedition in the history of Marine Sciences was

QUESTION ID:140

Paralytic shellfish poisoning (PSP) is caused by the consumption of molluscan Shellfish contaminated with

QUESTION ID:141

Biofilm/capsular material produced by the soil bacteria is detected by

QUESTION ID:142

A compound secreted by a soil bacterium capable of reducing the surface tension at air-water interface is called as

QUESTION ID:143

Match the organism in Group A with the appropriate habitat from Group B.

Group A                                   Group B

P) Pseudomonas                  i) Hot springs

Q) Thermus                           ii) Rhizosphere

R) Photobacterium               iii) Soil

S) Thiobacillus                      iv) Marine

QUESTION ID:144

Match the type of Organism in Group A with appropriate phenotype/property from Group B.
Group A                                                   Group B
P) Pseudomoans aeruginosa             i) Sulfur oxidizing organism
Q) Staphylococcus aureus                ii) Elemental N2 fixation
R) Thiobacillus ferroxidance            iii) Biofilm formation
S) Bradyrhizobium                            iv) Quorum sensing

QUESTION ID:145

Which one of the following reactions is performed by Cytochrome-P450 to detoxify the xenobiotics?

QUESTION ID:146

Which one of the following is NOT true regarding Zika virus?

QUESTION ID:147

The vaccine currently used in India’s Pulse polio campaign is

QUESTION ID:148

Which one of the following enzymes helps in the survival of Helicobacter pylori in the stomach?

QUESTION ID:149

Which one of the following is INCORRECT about Streptokinase?

QUESTION ID:150

The utility of positron emission tomography to diagnose tumors is based on

QUESTION ID:151

Which one of the following imaging techniques is non-invasive?

QUESTION ID:152

Amphotericin B is clinically used against which one of the following pathogens?

QUESTION ID:153

The therapeutic index (TI) indicates the relative margin of safety of a drug and it is deduced from the values of mean lethal dose (LD50) and median effective dose (ED50). Which of the following represents TI of a drug?

QUESTION ID:154

Which one of the following is a frequently used radio-opaque dye?

QUESTION ID:155

The name of the blood vessel marked [?] in the figure below is


QUESTION ID:156

Which one of the following is a reason for administering general anesthesia to experimental animals?

QUESTION ID:157

During the pathogenesis of rabies, the replication of virus first starts in the

QUESTION ID:158

Callipyge gene mutation in sheep leads to increased muscle growth. The gene presents a typical case of Polar over-dominance. Which of the four genotypes given below shows increased muscle growth? (N= wild type allele, C= mutant allele for callipyge)

QUESTION ID:159

The technique used in animal biotechnology for the rapid multiplication and production of animals with a desirable genotype is:

QUESTION ID:160

A primary culture of neurons isolated from the mouse brain responded positively when exposed to a chemical for a few seconds, but the neurons were less responsive and eventually stopped responding when exposed to the same chemical for prolonged duration. However, if the culture was left for some time and washed, the neurons started responding to the same chemical at the same concentration as before. The reduced response was due to

QUESTION ID:161

If a neuron is tyrosine hydroxylase positive, it could be either:

QUESTION ID:162

Pain sensation is a subjective and conscious feeling. However, although the autonomic organs viz. brain, heart etc. do not get represented in the cerebral cortex, one feels pain in those parts as well. The reason is

QUESTION ID:163

Neurons are formed from precursor proneural cells due to

QUESTION ID:164

A neuron at resting state when treated with “X” showed transmembrane potential -50mV, while when treated with “Y” it showed -90mV. Given such a condition, which of the following statements would be most appropriate?

QUESTION ID:165

Route of entry for canine distemper virus is

QUESTION ID:166

In a population which is in Hardy-Weinberg equilibrium, the frequency of a recessive allele for a certain genetic trait is 0.40. What percentage of individuals would be expected to show the dominant trait in the next generation?

QUESTION ID:167

Color blindness in human is a sex-linked recessive trait. If two individuals with normal color vision have a color blind son, what will be the genotypes of the parents?

QUESTION ID:168

A human is phenotypically female, but her interphase somatic nuclei do not display the existence of Barr bodies. Which of the following conditions could explain the above feature?

QUESTION ID:169

For the pedigree depicted below, which mode(s) of inheritance CANNOT be possible?

QUESTION ID:170

C banding of human chromosomes specifically reveals

QUESTION ID:171

Which one of the following expressions is likely to retrieve more matches in a database search?

QUESTION ID:172

Which substitution matrices should you prefer to find distantly related orthologs through BLAST search?

QUESTION ID:173

Match the items in Group 1 with an appropriate description in Group 2
Group I                                      Group 2
P. UPGMA                         1. Protein sequence database
Q. CLUSTALW                  2. Phylogenetic Analysis
R. SWISS-PROT              3. 3-D structure visualization
S. RasMol                         4. Multiple sequence alignment

QUESTION ID:174

The rate of substitutions in a certain region of DNA of length 1000 bases is estimated as 10-9 per base per year. If two species diverged approximately 10 million years ago, the fraction of sites that differ between them should be approximately

QUESTION ID:175

When searching the Blocks and PRINTS databases, a match is judged significant if

QUESTION ID:176

Two species are found to share a cluster of 8 genes, but the genes are in different orders in the two species. The orders are represented by signed permutations as given below:
Species X 1,2,3,4,5,6,7,8
Species Y 1, 2,-5,-4,-3,8,6,7
The transformation between the two gene orders

QUESTION ID:177

As the E value of a BLAST search becomes smaller

QUESTION ID:178

In a genome with average GC-content 40%, the expected frequency of occurrence of the tetranucleotide AACG is

QUESTION ID:179

Consider that you have collected X-ray diffraction data on three different protein crystals, referred to as Protein A, B and C. Data set for crystal A has the best diffraction spot at 8A, crystal B at 5A and crystal C has the best diffraction at 3A. Which data set is likely to yield a higher resolution crystal structure?

QUESTION ID:180

In a protein crystallization experiment, you have identified an optimal protein concentration and precipitant concentration which yield crystals. Now if you are asked to grow crystals again with a protein solution that is four times more concentrated, how will you alter the precipitant concentration?

QUESTION ID:181

Which of the following statements is true for two different tripeptides consisting of either glycine or proline?

QUESTION ID:182

In a Fluorescence Resonance Energy Transfer (FRET) experiment, the donor is Cyan Fluorescent Protein (CFP) which has excitation and emission maxima at 435 and 485 nm, respectively. The acceptor in this experiment is Yellow Fluorescent Protein (YFP) that has excitation and emission maxima at 513 and 545 nm, respectively . Under the conditions, where a significant FRET is observed, what will happen to the FRET signal if the emission maxima of CFP is made to shift to 475nm.

QUESTION ID:183

Consider that the following polypeptides are being analyzed by Mass spectrometry (MS). These peptides are generated by proteolytic cleavage of a protein that is expressed in mammalian cells and it has undergone glycosylation, phosphorylation and acetylation. What will the expected positioning of the spectra corresponding to these peptides (smaller to larger)?

QUESTION ID:184

Assuming that the sequence of CDRs of an antibody are heavily enriched with Tyrosine and Serine, what is likely to be the driving force stabilizing its interaction with the antigen?

QUESTION ID:185

Hydrophobic amino acids are prevalent in transmembrane regions of membrane embedded proteins because

QUESTION ID:186

Bryostatin is an anticancer agent obtained from

QUESTION ID:187

Synthesis of White spot syndrome virus envelop takes place in

QUESTION ID:188

Primary etiology of Epizootic Ulcerative Syndrome is a Fungus Aphanomyces invadans. Its zoospores have the property to

QUESTION ID:189

Gonad Inhibiting Hormone (GIH) in crustaceans is synthesized in

QUESTION ID:190

The synthetic equivalent of neuro-pharmacologically active peptides obtained from the marine snail Conus magus is

QUESTION ID:191

Viral encephalopathy and retinopathy in fishes are caused by

QUESTION ID:192

A pigment isolated from marine red algae that finds application in flow cytometry is

QUESTION ID:193

Which of the following electron acceptor that is used by the bacteria is mainly responsible for marine corrosion?

QUESTION ID:194

Which one of the following bacterial species is responsible for the Scombroid fish poisoning?

QUESTION ID:195

The larval stage of Macrobrachium rosenbergii is referred to as

QUESTION ID:196

The group of marine algae which have urea cycle is

QUESTION ID:197

Which of the following algae is responsible for the red color of RED SEA?

QUESTION ID:198

An oligotrophic lake has

QUESTION ID:199

After sodium chloride, which one of the following compounds has the maximum concentration in sea water?

QUESTION ID:200

Which of the following is an example of an intellectual property right?