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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Which of the following is true for acetyl-CoA?

QUESTION ID:2

The role of DNA ligase in DNA replication is

QUESTION ID:3

A buffer contains 10% glucose, 20 mM Tris, and 50 mM HCI. For making 1 litre of buffer from the following stock solutions – 50% glucose, 1M Tris, and 1 M HCI, the correct combination of volume each of the stock solutions will be

QUESTION ID:4

In enzyme kinetics, if the enzyme concentration in doubled

QUESTION ID:5

During growth, the diameter of a Staphylococcus bacterial cell increases by 5%. The specific surface area (defined as surface area per unit volume)

QUESTION ID:6

The enzyme used in glucometers to estimate blood glucose levels is

QUESTION ID:7

Which of the following is a method of investigating the sequence specificity of DNA- binding proteins in vitro?

QUESTION ID:8

What is the correct value of X?


QUESTION ID:9

Using only random VDJ recombination, from 40 V, 30 D and 6 J gene segments, the number of possible variable regions of the resulting antibody would be

QUESTION ID:10

The fruit of a particular tree species formed the predominant diet of the dodo. After dodo became extinct, that tree species also became extinct. Which of the following is the most likely cause for the tree’s extinction?

QUESTION ID:11

What is the frequency with which a 4bp cutter will cut the DNA, assuming random distribution of base in the genome?

QUESTION ID:12

Keshav and Kunal are good in Maths and Chemistry. Sumit and Keshav are good in Maths and Biology. Vineet and Kunal are good in Cricket and Chemistry. Sumit, Vineet and Rohit are good in Football and Biology. Who is good in Biology, Cricket, Chemistry and Football?

QUESTION ID:13

The molecular weight of Val and Ser are 117 Dalton and 105 Dalton, respectively. Val and Ser form a dipeptide Val-Ser. The molecular weight (in Daltons) of the dipeptide is

QUESTION ID:14

Which of the following is NOT a rational grouping of amino acids bases on their polarity properties?

QUESTION ID:15

Mr. Thomas invested an amount of Rs. 13,900 divided in two different schemes A and B at the simple interest rate of 14% p.a. and 11% p.a., respectively. If the total amount of simple interest earned in 2 years was Rs. 3508, what was the amount invested in Scheme B?

QUESTION ID:16

Someone tells you that the pH of a solution is minus 2. Which one of the following is false?

QUESTION ID:17

How much sodium hydroxide will you weigh to prepare 0.25 L of 3 M solution?

QUESTION ID:18

Which of the following is/are critical for genome replication?

QUESTION ID:19

A student made 0.15 M solution of copper sulphate. The absorbance of the solution was found to be 0.3 when usinga cuvette with a path length of 1 cm. Copper sulphate solution made by a second student gave an absorbance of 0.45 using the same cuvette at the same wavelength. What is the concentration of the copper sulphate solution made by the second student?

QUESTION ID:20

Choose the correct alternative from the series on the right to complete the missing figure in the series on the left.

QUESTION ID:21

Bacteria protect themselves from phages by producing the following enzymes which fragment the phage genome

QUESTION ID:22

Two particles are moving back and forth in a 10 m long tube. Particle ‘P’ is moving at a speed of 5m/s and particle ‘Q’ at a speed of 2 m/s. Consider that Both P and Q start at the same time in the same direction. How much times will ‘P’ cross ‘Q” by the time ‘Q’ reaches the end of the tube?

QUESTION ID:23

The most important step of an automated DNA sequencing reaction is

QUESTION ID:24

Chlorine is assigned and atomic weight of 35.5. This is due to

QUESTION ID:25

The number of squares and triangles in the following figure is

QUESTION ID:26

Histones

QUESTION ID:27

A mixture of homotetramer ‘X’ and heterodimer ‘Y’ with identical molecular weight were resolved on SDS-PAGE. It gives three bands on a gel with molecular weights 40 kDa, 60 kDa, and 100 kDa, The native molecular wight (in kDa) of the homotetramer ‘X’ is

QUESTION ID:28

Find the next number in the series 23, 30, 38, 47, 57

QUESTION ID:29

What is the probability of getting 53 Sundays in a ‘Leap’ year?

QUESTION ID:30

Synthesis of majority of lipids in a cellular system occurs in the

QUESTION ID:31

What will be the generation time of a culture with a specific growth rate constant of 0.01 𝒎𝒊𝒏−𝟏 ?

QUESTION ID:32

Ligands ‘A’ and ‘B’ bind to protein ‘P’ with dissociation constants of 1 nM and 100 nM, respectively. Which of the following true?

QUESTION ID:33

The Freund’s complete adjuvant is a mixture of

QUESTION ID:34

The aluminium bronze alloy consists of copper and aluminium in the ratio of 10:1 by weight. If an object made of this alloy weighs 77 Kilograms (Kg), how many Kg of aluminium does it contain?

QUESTION ID:35

Primary cilia biogenesis typically starts at the

QUESTION ID:36

Which of the following is NOT true ?

QUESTION ID:37

Trypsin cleaves a protein at the

QUESTION ID:38

A, B, C, and D are to be seated in a row, But C and D cannot be together. Also B cannot be at the third place. Which of the following must be false?

QUESTION ID:39

Single stranded DNA can be separated from double stranded DNA efficiently using

QUESTION ID:40

Eight 3rd year students can finish an experiment in 15 days and eighteen 1st year students can complete the same experiment in 10 days. If all these students work together, in how many days will the experiment get completed?

QUESTION ID:41

The temperature of media post sterilization drops from 100°C to 60°C in 40 min by simply keeping it on the lab bench and allowing slow atmospheric cooling to take place at an ambient temperature of 20°C. In the next 40 min, the approximate temperature (°C) of the media would be around

QUESTION ID:42

Which of the following is a non-reducing sugar?

QUESTION ID:43

Denaturation of DNA is a

QUESTION ID:44

The egg white protein, ovalbumin, is denatured in a hard-boiled egg. Which of the following i least affected?

QUESTION ID:45

Enzymes bind their substrates via

QUESTION ID:46

Nucleic acid structures are stabilized by

QUESTION ID:47

How many peptide fragments can be generated from the complete digestion of the polypeptide AGRCDKCQANRSLMNF with trypsin?

QUESTION ID:48

While working in the lab, you forgot to keep enzymes back in the fridge. Which of the following enzyme will be least affected on being left outside at room temperature?

QUESTION ID:49

The genetic codon is a triplet and there are 64 codons. How many codons would be possible if the codon is a doublet?

QUESTION ID:50

In the first semester course work at the Biotech Institute, 50 students signed up for both Genetics and Statistics, and 90 students signed up for either Genetics or Statistics. If 25 students are taking Genetics but are not taking Statistics, how many students are taking Statistics but not taking Genetics?

QUESTION ID:51

Opsonization is the process of

QUESTION ID:52

Fragile X syndrome is caused by a fragile site at the end of the long arm of X chromosome. Such a disorder is

QUESTION ID:53

Which of the following is NOT an arboviral infection?

QUESTION ID:54

The enzyme-linked immunospot (ELISPOT) assay is highly sensitive because it can measure

QUESTION ID:55

During Agrobacterium infections, plant cell begins to synthesize Arginine derivatives called

QUESTION ID:56

Which of the following disorders does not show sex-linked inheritance?

QUESTION ID:57

What is Endoreduplication?

QUESTION ID:58

Which of the following are NOT transcribed by RNA polymerase II ?

QUESTION ID:59

Hepatitis B is caused by a

QUESTION ID:60

Digestion of a 5 Kb linear DNA with BamHi leads to the generation of two fragments of size 2 Kb and 3 Kb, while digestion of the same DNA with Hindill generates 3 fragments of 0.7, 0.8 and 3.5 kb. When the same DNA is cut with both BamHI and Hindill enzymes the fragments generated are of 0.7, 0.8, 1.3 and 2.2 Kb. The right order of the recognition sites for the two enzymes is

QUESTION ID:61

The probe used to analyse glycoproteins is

QUESTION ID:62

The mature anther wall comprises an epidermis followed by a layer of radially elongated cells with fibrous bands of callose called endothecium whose function is

QUESTION ID:63

Proteins are commonly purified by ion exchange chromatography (IEC) as a final step. Which of the following statements is NOT true?

QUESTION ID:64

For a microbial culture, if the doubling time is 0.231 h, the specific growth rate (in h-1) will be (assume that the endogenous metabolism is negligible)

QUESTION ID:65

Which one of the following involves RNA Editing?

QUESTION ID:66

The following cellular process involves formation of double membrane vesicles that engulf and degrade the cellular organelles and macromolecules

QUESTION ID:67

If a nonsense mutation is present in the Lacz gene of the lac operon, the mRNA of β- galactosidase would

QUESTION ID:68

The first drug approved by FDA of USA, that was produced through genetic engineering was

QUESTION ID:69

A shuttle vector is a vector that

QUESTION ID:70

Which of the following represents a quantitative measure of the structural similarity between two proteins?

QUESTION ID:71

Golden rice' is genetically engineered by altering the biosynthetic pathway for the production of

QUESTION ID:72

Which part of a plant would be most suitable for raising virus-free plants for micropropagation?

QUESTION ID:73

Which one of the following can be extended by Klenow enzyme upon addition of dNTPs and Mg2+?

QUESTION ID:74

Wetlands are very rich and diverse ecosystems and must be preserved. Which convention signed in Iran protects this specific ecosystem (wetlands) on a global basis?

QUESTION ID:75

Which post-translational modification is observed most commonly in signal transduction? 

QUESTION ID:76

In the cloverleaf structure of tRNA, the cognate amino acid is attached at

QUESTION ID:77

The mucopolysaccharide hyaluronic acid is composed of

QUESTION ID:78

In the DNA helix, the GC and AT base pairs

QUESTION ID:79

The equation for aerobic production of acetic acid from ethanol is:
CH2OH+O2→CH3CO2H+H2O.
(ethanol) (acetic acid)
Acetobacter aceti bacteria are added to vigorously-aerated medium containing 10 g.14 ethanol. After some time, the ethanol concentration is 2 g.-2 and 7.5 g.11 of acetic acid is produced. What is the observed yield (Yoing-) and theoretical yield (Yting-8") of acetic acid from ethanol?

QUESTION ID:80

Which of the following floral whorls are absent in agamous (ag) mutant of Arabidopsis?

QUESTION ID:81

The biological sample used for diagnosis of Giardiasis is

QUESTION ID:82

The primary structure of a protein is stabilized by

QUESTION ID:83

The malarial parasite that has caused recent outbreaks of Monkey malaria in humans is

QUESTION ID:84

Embryonic stem cells are

QUESTION ID:85

Which of the following represents the correct sequence of steps in pathogenesis?

QUESTION ID:86

Which of the following is a chemotherapeutic drug obtained from marine source?

QUESTION ID:87

Which of the following marine phototrophs can utilise carbon-dioxide as the carbon source?

QUESTION ID:88

The amino acid residue with the least preference for any quadrant in the Ramachandran map is

QUESTION ID:89

In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 30 𝒈𝑳−𝟏(𝑫𝑪𝑾), the specific growth rate (𝝁) is 𝟎.𝟒𝒉−𝟏 and substate uptake rate (𝒒) is 𝟏𝟖𝒈𝑳−𝟏𝒉−𝟏. The cell nyield coefficient 𝒀𝒙/𝒔 will be

QUESTION ID:90

Which class of phytochromes is highly abundant in etiolated seedlings and is also light labile?

QUESTION ID:91

The broad- spectrum herbicide glyphosate, the active ingredient of Roundup, inhibits this enzyme

QUESTION ID:92

Which histone is NOT a part of the nucleosomes?

QUESTION ID:93

For a tetranucleotide sequence, the number of possible combination using A, T and G are

QUESTION ID:94

Which of the following amino acids is most likely to disrupt an 𝜶−𝒉𝒆𝒍𝒊𝒙?

QUESTION ID:95

BLOSUM matrix is used for

QUESTION ID:96

In a plants , the cell adjacent to the egg cell in an ovule are known as

QUESTION ID:97

Small/short interfering RNA (siRNA) is a commonly used RNA tool that causes

QUESTION ID:98

Synthesis of which of the following liquids is completed in the Golgi bodies

QUESTION ID:99

Which type of post- translational modification does not occur in plastids?

QUESTION ID:100

To maintain soil fertility, the most sustainable agricultural practise is

QUESTION ID:101

Sam was investigating impact of lactate dehydrogenase knockout on glycolytic pathway. What will be the net NADH production that he would expect in conversion of Glucose to pyruvcate in this case?

QUESTION ID:102

Which of the following plant horomones employs a phosphorelay system to regulate gene expression?

QUESTION ID:103

Which of the following is associated with SARS-CoV-2 infection?

QUESTION ID:104

5thjune is observed as

QUESTION ID:105

If neurons that produce the neurotransmitter dopamine could be generated from stem cells grown in culture, it might be possible to treat patient suffering with

QUESTION ID:106

One centimorgan is defined as the genetic distance between two loci with a statistically corrected recombination frequency of

QUESTION ID:107

The method utilized to determine the three dimentional structure of proteins are

QUESTION ID:108

The Innate immune system recognizes Pathogen Associated Molecular Patterns (PAMPs) through activation of

QUESTION ID:109

Two film theory of mass transfer considers

QUESTION ID:110

Two amino acids with negatively charged side chains are

QUESTION ID:111

Which of these procedures poses the least risk to an unborn child?

QUESTION ID:112

Which of the combination of gases is finally produced during anaerobic digestion?

QUESTION ID:113

Phylogenetic tree provides information about

QUESTION ID:114

Which of the following processes is used to produce biodiesel?

QUESTION ID:115

Which of the following organelle is NOT a site for Reactive Oxygen Species (ROS) generation?

QUESTION ID:116

A stirred tank bioreactor of 𝟐.𝟕𝒎𝟑 is agitated using a Rushton turbine with diameter 0.5 m and stirrer speed of 𝟏 𝒔−𝟏. If the fermentation broth has viscosity and density of 𝟏𝟎−𝟐 𝒑𝒂.𝒔 and 𝟏𝟎𝟎𝟎𝒌𝒈.𝒎−𝟑 respectively, the mixing time (in seconds) for the bioreactor will be

QUESTION ID:117

The 𝜷−𝒔𝒉𝒆𝒆𝒕 rich structure of prion protein represents the

QUESTION ID:118

The fluid property, due to which, mercury does not wet the glass is

QUESTION ID:119

A major organism used in commercial bioleaching for copper recovery is

QUESTION ID:120

A covalently closed circular DNA was in a relaxed state in water at 300C. What will happen if the water temperature increases to 600C or decreases to 100C?

QUESTION ID:121

Pruning helps in making the hedge dense because

QUESTION ID:122

The location of a proteins in cells can be studied using

QUESTION ID:123

A fermentation medium is being cooled from 700C to 320C in a double pipe heat exchanger. Fluid flowing counter currently with this stream in heated from 200C to 460C. The log mean temperature difference (in0C) for the two streams is

QUESTION ID:124

In a DNA molecule, two antiparallel strands that are complementary in their nucleotide sequence are paired to form a

QUESTION ID:125

Consider a bacterium that grows with doubling time of 20 min in the exponential phase of its growth cycle and acquire 10 random mutrations in its genome in every generation. How many mutations will it acquire after 48 hr of growth in exponential phase?

QUESTION ID:126

During genome engineering process, the role of flippase enzyme in the next round of modification in the target gene is to

QUESTION ID:127

An energy generatioin process in which organic compounds act as both electron donors and terminal electron acceptor in a microbe is called

QUESTION ID:128

Which of the following process does NOT contribute to conversion of a proto-oncogene to oncogene?

QUESTION ID:129

The artificial sweetener, aspartame, is enzymatically produced using

QUESTION ID:130

The sequence CGAATTTGG is matched globally with four sequences in a database. The sequence that will give the highest similarity score taking match=1, mismatch=0 and gap penalty=minus1 is

QUESTION ID:131

In which organelle of seeds are stored oils converted to fatty acids and glycerol during germination?

QUESTION ID:132

Met-Ile-Val-His-Tyr was the sequence of a hypothetical peptide. Assuming that there are two possible codons each for His, val and Try, one possible codon for Met, and four possible codons for Ile, the number of possible nucleotide sequences coding for this peptide would be

QUESTION ID:133

What is the use of Aminopterin in hybridoma production?

QUESTION ID:134

How many linkage groups would be there in a plant with 2n=20?

QUESTION ID:135

A bacterial population, growing in batch culture, increases from 1000 cells to 10,00,000 cells in 5 hours. What is the generation time of the bacteria?

QUESTION ID:136

In large scale fermentation processes, corn steep liquor is mainly used as a

QUESTION ID:137

Aqueous two phase partitioining (ATPS) is used for the recovery of an enzyme from the cell free culture fitrate. On addition of PEG-2000 and dextran, the mixture separates into two phases with a partition coefficient for the enzyme as 4.2. The maximum possible enzyme recovery, when the volume ratio of the upper to lower phases in 5.0, will be

QUESTION ID:138

Which interactions are generally observed at the core of stable protein-protein complexes?

QUESTION ID:139

The genetic disease familial Hypercholesterolemia that leads to an increase in blood cholesterol is caused due to

QUESTION ID:140

If four atoms A, B, C, and D are connected linearly and there is rotation possible along bond BC, dihedral angel on this bond is described as

QUESTION ID:141

Computational Prediction of protein folding assumes that

QUESTION ID:142

The Budapest Treaty related to the international patent process concern with

QUESTION ID:143

What is agent Orange?

QUESTION ID:144

Match the polysaccharides (L.H.S.) with the microbial cultures (R.H.S.) associated with their industrial production

QUESTION ID:145

The comparison of the structures of haemoglobin and myoglobin shows that they have

QUESTION ID:146

All the vaccines mentioined below are attenuated or inactivated whole pathogen except

QUESTION ID:147

A combination of gametes that can be formed by the genotype AaBbCcDdEeFfGg are

QUESTION ID:148

Which of the following microorganism is used for commercial production of dextran?

QUESTION ID:149

dilution of a microbial culture was prepared by adding 1 ml of the culture to 9 ml of sterile blank. Further, 200 𝝁𝑳 from the diluted culture was spread on an agar plate; and 150 colonies were observed after the incubation period. Calculate the CFU/mL of the original sample

QUESTION ID:150

At what condition does the specific growth rate of the microorganisms decline in a constant volume fed-batch culture

QUESTION ID:151

Cyclosporine, as immunosuppressive drug, given to avoid transplant rejection acts by

QUESTION ID:152

Which of the following is not a suitable material for a depth filter used in air sterilization?

QUESTION ID:153

A protein with 1000 amino acids was tagged with GFP. The molecular weight of GFP is 26 KDa What will be the most likely molecular weight of the fused target protein?

QUESTION ID:154

The temperature (℃) of liquid nitrogen used for cryopreservation of plant samples is

QUESTION ID:155

Which of the following methods first ionizes a protein before separation and detection?

QUESTION ID:156

The first organic acid to be produced industrially is

QUESTION ID:157

In the design of a fermenter, which one of the following is NOT the intended use of baffles?

QUESTION ID:158

Which one of the following statements is true regarding the magnesium porphyrin ring and the phytol chain of a chlorophyll molecule?

QUESTION ID:159

Synthetic seeds are mostly derived from

QUESTION ID:160

A supramolecular complex that serves to degrade damaged or unneeded proteins in the cell is called as

QUESTION ID:161

A mixture of three proteins (X, Y, and Z) was loaded on a size exclusion column. The molecular weight (<W) and pl of the proteins are as follows

QUESTION ID:162

Baroreceptors are responsible for sensing human

QUESTION ID:163

ATP with 𝜸−𝟑𝟐p can be used for which of the following type of reaction?

QUESTION ID:164

Epicatechin gallate (ECG) is a type of flavonoid found in which of the following?

QUESTION ID:165

What are the cellular sites for protein glycosylation?

QUESTION ID:166

'Cybrids' are produced by

QUESTION ID:167

Two proteins of molecular weights 1.0 x 105 and 1.0 x 104 Daltons were eluted from a gel filtration column at 220 ml and 300 ml respectively. The molecular weight of an unknown protein that elutes at 140 ml under the same conditions, will be

QUESTION ID:168

Sequence-specific recognition of DNA by proteins occurs primarily through the

QUESTION ID:169

Choose the correct order in terms of pka

QUESTION ID:170

Which of the following is NOT a feature of bacterial DNA replication?

QUESTION ID:171

A student added a 5' exonuclease enzyme instead of a restriction enzyme to digest his purified plasmid DNA sample. What is he likely to observe when he runs his plasmid digest on an agarose gel?

QUESTION ID:172

A CDNA encoding a human protein of interest was cloned in a bacterial expression vector and introduced into bacterial cells for expression. However, no expression of the human protein of interest was obtained. This could be because of

QUESTION ID:173

Only 10 % of babies with Edward syndrome survive beyond 5 years. This is a genetic disease arising due to

QUESTION ID:174

Which autoimmune disease is caused by production of autoantibodies and autoreactive T cells against DNA and chromatin proteins?

QUESTION ID:175

Immobilization of enzymes to water insoluble, anionic porous carriers often results in an apparent shift in the pH optima of the enzyme. The physico-chemical interaction likely to cause such a behaviour is

QUESTION ID:176

Nucleosides isolated from a Caribbean sponge, Cryptotethya crypta, were the basis for the synthesis of the antiviral

QUESTION ID:177

What is Single Nucleotide Polymorphism (SNP)?

QUESTION ID:178

Accuracy of Protein Structure Prediction can be assessed using tools like

QUESTION ID:179

A class of temperature sensitive E. coli mutants defective in DNA replication were identified that ceased replication immediately upon increase in temperature. Which of the following processes are likely to be defective in these mutants?

QUESTION ID:180

A set of closely linked genetic markers present on a single chromosome, which are not easily separable by recombination and tend to be inherited together are termed as

QUESTION ID:181

Cellulose is a linear polymer of glucose with

QUESTION ID:182

The specific energy source for the reaction ADP + phosphate → ATP by the enzyme ATP synthetase (CF1 Coupling Factor) in thylakoid membranes is

QUESTION ID:183

Which of the following is NOT used for producing vitamins industrially?

QUESTION ID:184

In Transmission Electron Microscope (TEM), a beam of electrons interact with the specimen to form image as

QUESTION ID:185

Which one of the following approaches is generally not used for identifying an SNP?

QUESTION ID:186

DNA glycosylases are DNA repair enzymes involved in

QUESTION ID:187

What will be the molarity of a 4 mg/ml solution of NaOH?

QUESTION ID:188

A humanised antibody is one in which the

QUESTION ID:189

Identify the incorrect pair

QUESTION ID:190

Genes related through vertical descent from a common ancestral gene are called

QUESTION ID:191

Which of the following is NOT used as a biopesticide?

QUESTION ID:192

Which of the following does not have a quaternary structure?

QUESTION ID:193

The technique used to locate specific genes in chromosomes

QUESTION ID:194

Which is the correct arrangement of the polarity of solvents?

QUESTION ID:195

A protein of 100 KDa would have approximately how many amino acids?

QUESTION ID:196

Which of following is a trisaccharide?

QUESTION ID:197

The first stable product of C3 cycle is

QUESTION ID:198

Which of the following yeast is used for the production of riboflavin?

QUESTION ID:199

Ananda Chakrabarty received the first U.S. patent for a GM organism. This organism was

QUESTION ID:200

Deficiency of this macronutrient causes older leaves to turn dark green or reddish purple