TLS Online

Previous Year Questions

Performance Meter

TLS Online TPP Program

QUESTION ID:1

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r).

Assertion: N-methyl-N' -nitro-N-nitrosoguanidine (NTG) is an effective chemical mutagen.

Reason: Mutations induced by NTG mainly are the GC ~ AT transitions.

both (a) and (r) are true and (r) is the correct reason for (a)

both (a) and (r) are true but (r) is not the correct reason for (a)

(a) is true but (r) is false

(a) is false but (r) is true

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13356&year=2011&month=February

QUESTION ID:2

Determine the correctness of the following statements

I. Enhancer sequences are those DNA sequences that are involved in increasing the rate of DNA replication.

II. Enhancer sequences work by binding with eukaryotic gene activator factors.

only I is true

only II is true

both I and II are true

both I and II are false

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13357&year=2011&month=February

QUESTION ID:3

In a well aerated and agitated microbial culture, the 'supply' of oxygen is equal to 'demand' (uptake) of the growing culture. The KLa for such a system will be (KLa = volumetric mass transfer coefficient, c* = dissolved oxygen concentration in liquid in equilibrium with gaseous oxygen, C = instantaneous value of dissolved oxygen concentration, 'r' = specific oxygen uptake rate per unit weight of cells, X = dry weight of the cells per unit volume)

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13358&year=2011&month=February

QUESTION ID:4

Structured William's model

P. can describe the changes in intracellular components of the cell during growth

Q. can not describe the death phase of the cells

R. can describe the variation of size of cells in the different phases of growth

S. can not describe the lag period of growth

Which one of the following is CORRECT?

P, Q and S only

P, Q and R only

Q, R and S only

P, RandS only

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13359&year=2011&month=February

QUESTION ID:5

Match items in Group I with Group II.

Group I	Group II
P. Glycolytic pathway	1. Chloroplast
Q. Eukaryotic oxidative metabolism	2. Glyoxysomes
R. Glyoxylate cycle	3. Mitochondria
S. Calvin cycle	4. Cytosol

P-1, Q-2, R-3, S-4

P-2, Q-3, R-4, S-1

P-4, Q-3, R-2, S-1

P-3, Q-4, R-1, S-2

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13360&year=2011&month=February

QUESTION ID:6

Match items in Group I with Group II.

Group I	Group II
P. Alzheimer’s disease	1. H1N1
Q. Mad cow disease	2. Hemoglobin
R. Sickle cell anaemia	3. Prions
S. Swine flu	4. Amyloid

P-4, Q-3, R-2, S-1

P-3, Q-4, R-1, S-2

P-2, Q-1, R-4, S-3

P-1, Q-2, R-3, S-4

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13361&year=2011&month=February

QUESTION ID:7

Determine the corr-ectness or otherwise of the following Assertion (a) and the Reason (r)

Assertion: The elucidation of ribosome structure helps in the development of new generation drugs.

Reason: The high resolution of macromolecular structure has enabled in structure-based drug design.

both (a) and (r) are true and (r) is the correct reason for (a)

both (a) and (r) are true but (r) is not the correct reason for (a)

(a) is true but (r) is false

(a) is false but (r) is true

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13362&year=2011&month=February

QUESTION ID:8

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)

Assertion : A very low amount of inhibitor can act as an activator for allosteric enzymes.

Reason: Alloste1ic enzymes follow Michaelis-Menten kinetics.

both (a) and (r) are true and (r) is the correct reason for (a)

both (a) and (r) are true but (r) is not the correct reason for (a)

(a) is true but (r) is false

(a) is false but (r) is true

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13363&year=2011&month=February

QUESTION ID:9

Match the terms in Group I with their associated functions in Group II.

Group I	Group II
P. Shine-Dalgarno sequences	1. Aminoacylation of tRNA
Q. Leucine zipper	2. Gene silencing
R. Aminoacyl tRNA synthetase	3. Ribosome binding and facilitation of translation initiation
S. RNA interference (RNAi)	4. Transcription factors

P-3, Q-4, R-1, S-2

P-4, Q-3, R-2, S-1

P-2, Q-3, R-1, S-4

P-3, Q-2, R-4, S-1

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13364&year=2011&month=February

QUESTION ID:10

Protein-protein interactions are studied by

P. DNA foot printing

Q. Yeast two hybrid system

R. Ligase chain reaction

S. Mass spectrometry

P and S only

Q and S only

P and R only

Q and R only

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13365&year=2011&month=February

QUESTION ID:11

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)

Assertion : Isopropylthiogalactoside (IPTG) is a gratuitous inducer of lactose operon.

Reason: Gratuitous inducers are chemical analogs which behave like natural inducer but they do not serve as substrate for the enzymes that are subsequently synthesized.

both (a) and (r) are true and (r) is the correct reason for (a)

both (a) and (r) are true but (r) is not the correct reason for (a)

(a) is true but (r) is false

(a) is false but (r) is true

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13366&year=2011&month=February

QUESTION ID:12

Determine the con·ectness or otherwise of the following Assertion (a) and the Reason (r)

Assertion : In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously.

Reason : Synchronous culture could be obtained by starving cells for essential nut1ient components.

both (a) and (r) are true and (r) is the correct reason for (a)

both (a) and (r) are true but (r) is not the correct reason for (a)

(a) is true but (r) is false

(a) is false but (r) is true

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13367&year=2011&month=February

QUESTION ID:13

Which of the following characteristics with respect to bacterial DNA polymerase III are TRUE?

P. Initiation of chain synthesis

Q. 5'- 3' polymerization

R. 3'- 5' exonuclease activity

S. 5'- 3' exonuclease activity

P and Q only

Q and R only

Q and S only

P and S only

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13368&year=2011&month=February

QUESTION ID:14

Maximum specific growth rate (!lmax) of a microorganism is calculated by taking the (ln=loge, X=biomass, t =time)

slope of In X vs t of the growth cycle

slope of In X vs t during the exponential growth phase

slope of X vs t

slope of X vs t during the exponential phase of growth

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13369&year=2011&month=February

QUESTION ID:15

Identify the CORRECT statements

P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction

Q. S1 nuclease can cleave the DNA strand of a DNA-RNA hybrid

R. T 4 polynucleotide kinase is used for labeling 3' end of DNA

S. Baculovirus (Autographa californica) can be used as an insect expression vector

P and Q only

R and S only

P and S only

Q and R only

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13370&year=2011&month=February

QUESTION ID:16

Value of the determinant mentioned below is

-30

-24

-10

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13371&year=2011&month=February

QUESTION ID:17

HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following

I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine.

II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.

only I is true

only II is true

both I and II are true

I is true and II is false

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13372&year=2011&month=February

QUESTION ID:18

Red-green colour blindness is inherited as a recessive X-linked trait.

What will be the probability of having the colour-blind son to a woman with phenotypically normal parents and a colour-blind brother, and married to a normal man? (Assume that she has no previous children)

100%

50%

25%

12.5%

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13373&year=2011&month=February

QUESTION ID:19

Red-green colour blindness is inherited as a recessive X-linked trait.

What will be the probability of having the colour-blind daughter to a phenotypically normal woman, who already had one colour-blind son, and is married to a colour-blind man?

75%

50%

25%

15%

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13374&year=2011&month=February

QUESTION ID:20

A microorganism grows in a continuous 'chemostat' culture of 60 m^3 working volume with sucrose as the growth limiting nutrient at dilution rate, D = 0.55 h^- 1 . The steady state biomass concentration is 4.5 Kg dry biomass m^·3 and the residual sucrose concentration is 2.0 Kg m^·3. The sucrose concentration in the incoming feed medium is 10.0 Kg m·^3

What would be the yield YXJs (Kg biomass/Kg substrate)?

0.562

0.462

0.362

0.162

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13375&year=2011&month=February

QUESTION ID:21

What would be the sucrose concentration in the input feed for the output to be 45 Kg biomass h^- 1?

3.225 Kg m^·3

4.425 Kg m^·3

5.115 Kg m^·3

6.525 Kg m^-3

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13376&year=2011&month=February

QUESTION ID:22

The abdomen length (in millimeters) was measured in 15 male fruit flies, and the following data were obtained: 1.9, 2.4, 2.1, 2.0, 2.2, 2.4, 1.7, 1.8, 2.0, 2.0, 2.3, 2.1, 1.6, 2.3 and 2.2.

Vmiance (Vx) for this population of fruit flies as calculated from the above data shall be

0.85

0.25

0.061

0.08

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13377&year=2011&month=February

QUESTION ID:23

The abdomen length (in millimeters) was measured in 15 male fruit flies, and the following data were obtained: 1.9, 2.4, 2.1, 2.0, 2.2, 2.4, 1.7, 1.8, 2.0, 2.0, 2.3, 2.1, 1.6, 2.3 and 2.2.

The value of standard deviation (SD) will be

0.061

0.25

0.61

0.85

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13378&year=2011&month=February

QUESTION ID:24

A 200 µl of polymerase chain reaction has 100 template DNA molecules and the reaction was performed for 10 cycles.

How many molecules of amplicons will be generated?

1.024 x 10⁴

1.024 x 10⁵

2.048 x 10⁴

2.048 x 10⁵

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13379&year=2011&month=February

QUESTION ID:25

A 200 µl of polymerase chain reaction has 100 template DNA molecules and the reaction was performed for 10 cycles.

How many molecules of amplicons will be present in 0.1 µl of reaction?

102.4

1024

51.2

512

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13380&year=2011&month=February

QUESTION ID:26

Which of the following options is the closest in the meaning to the word below:

Inexplicable

Incomprehensible

Indelible

Inextricable

Infallible

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13381&year=2011&month=February

QUESTION ID:27

Choose the word from the options given below that is most nearly opposite in meaning to the given word:

Amalgamate

merge

split

collect

separate

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13382&year=2011&month=February

QUESTION ID:28

Choose the most appropriate word from the options given below to complete the following sentence.

If you are trying to make a strong impression on your audience, you cannot do so by being understated, tentative or _____ _

hyperbolic

restrained

argumentative

indifferent

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13383&year=2011&month=February

QUESTION ID:29

Choose the most appropriate word(s) from the options given below to complete the following sentence.

I contemplated ____ Singapore for my vacation but decided against it.

to visit

having to visit

visiting

for a visit

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13384&year=2011&month=February

QUESTION ID:30

If Log (P) = (l/2)Log (Q) = (1/3) Log (R), then which of the following options is TRUE?

P^2 = Q^3R^2

Q^2 = PR

Q^2 = R^3P

R = P^2Q

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13385&year=2011&month=February

QUESTION ID:31

Few school curricula include a unit on how to deal with bereavement and grief, and yet all students at some point in their lives suffer from losses through death and parting.

Based on the above passage which topic would not be included in a unit on bereavement?

how to write a letter of condolence

what emotional stages are passed through in the healing process

what the leading causes of death are

how to give support to a grieving friend

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13386&year=2011&month=February

QUESTION ID:32

A container originally contains 10 litres of pure spirit. From this container 1 litre of spirit is replaced with 1 litre of water. Subsequently, 1 litre of the mixture is again replaced with 1 litre of water and this process is repeated one more time. How much spirit is now left in the container?

7.58 litres

7. 84 litres

7 litres

7.29 litres

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13387&year=2011&month=February

QUESTION ID:33

A transporter receives the same number of orders each day. Cunently, he has some pending orders (backlog) to be shipped. If he uses 7 trucks, then at the end of the 4th day he can clear all the orders. Alternatively, if he uses only 3 trucks, then all the orders are cleared at the end of the 4th day. What is the minimum number of trucks required so that there will be no pending order at the end of the 5th day?

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13388&year=2011&month=February

QUESTION ID:34

The variable cost (V) of manufacturing a product varies according to the equation V= 4q, where q is the quantity produced. The fixed cost (F) of production of same product reduces with q according to the equation F = 100/q. How many units should be produced to minimize the total cost (V+F)?

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13389&year=2011&month=February

QUESTION ID:35

P, Q, RandS are four types of dangerous microbes recently found in a human habitat. The area of each circle with its diameter printed in brackets represents the growth of a single microbe surviving human immunity system within 24 hours of entering the body. The danger to human beings vaties proportionately with the toxicity, potency and growth attributed to a microbe shown in the figure below:

A pharmaceutical company is contemplating the development of a vaccine against the most dangerous microbe. Which microbe should the company target in its first attempt?

https://triyambak.org/courses/gate-biotechnology/tpp/pyq?qid=13390&year=2011&month=February

Previous Year Questions

1.024 x 104

1.024 x 105

2.048 x 104

2.048 x 105

1.024 x 10⁴

1.024 x 10⁵

2.048 x 10⁴

2.048 x 10⁵