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Previous Year Questions

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TLS Online TPP Program

QUESTION ID:1

In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?

QUESTION ID:2

Choose the CORRECT combination of True (T) and False (F) statements about microcarriers used in animal cell culture.
P. Higher cell densities can be achieved using microcarriers
Q. Microcarriers increase the surface area for cell growth
R. Microcarriers are used for both anchorage- and nonanchorage-dependent cells
S. Absence of surface charge on microcarriers enhances attachment of cells

QUESTION ID:3

In an assay of the type II dehydroquinase of molecular mass 18 kDa, it is found that the Vmax of the enzyme is 0.0134 μmol.min^-1 when 1.8 μg enzyme is added to the assay mixture. If the Km for the substrate is 25 μM, the kcat/Km ratio will be ___________________×104 M^-1.^s-1.

QUESTION ID:4

The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 and 1280 M-1.cm-1, respectively. The polypeptide chain of yeast alcohol dehydrogenase (37 kDa) contains 5 Trp and 14 Tyr residues. The absorbance at 280 nm of a 0.32 mg.mL-1 solution of yeast alcohol dehydrogenase measured in a cuvette of 1 cm pathlength will be ________________.
(Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids in the yeast alcohol dehydrogenase).

QUESTION ID:5

The activity of lactate dehydrogenase can be measured by monitoring the following reaction:


The molar extinction coefficient of NADH at 340 nm is 6220 M^-1.cm^-1. NAD+ does not absorb at this wavelength. In an assay, 25 μL of a sample of enzyme  (containing 5 μg protein per mL) was added to a mixture of pyruvate and NADH to give a total volume of 3 mL in a cuvette of 1 cm pathlength. The rate of decrease in absorbance at 340 nm was 0.14 min^-1. The specific activity of the enzyme will be _______________ μmol.min^-1.mg^-1.

QUESTION ID:6

Analysis of a hexapeptide using enzymatic cleavage reveals the following result:
 Amino acid composition of the peptide is: 2R, A,V, S, Y
Trypsin digestion yields two fragments and the compositions are: (R, A, V) and (R, S, Y)
 Chymotrypsin digestion yields two fragments and the compositions are: (A, R, V, Y) and (R, S)
 Digestion with carboxypeptidase A yields no cleavage product.
Given: Trypsin cleaves at carboxyl side of R. Chymotrypsin cleaves at carboxyl side of Y. Carboxypeptidase A cleaves at amino side of the C-terminal amino acid (except R and K) of the peptide.
The correct amino acid sequence of the peptide is:

QUESTION ID:7

The empirical formula for biomass of an unknown organism is . To grow this organism, ethanol (C2H5OH) and ammonia are used as carbon and nitrogen sources, respectively. Assume no product formation other than biomass. To produce 1 mole of biomass from 1 mole of ethanol, the number of moles of oxygen required will be _______________.

QUESTION ID:8

Saccharomyces cerevisiae is cultured in a chemostat (continuous fermentation) at a dilution rate of 0.5 h^-1. The feed substrate concentration is 10 g.L^-1. The biomass concentration in the chemostat at steady state will be _____________ g.L^-1
Assumptions: Feed is sterile, maintenance is negligible and maximum biomass yield with respect to substrate is 0.4 (g biomass per g ethanol).
Microbial growth kinetics is given by 

where μ is specific growth rate (h-1), μm = 0.7 h^-1, Ks = 0.3 g.L^-1 and s is substrate concentration (g.L^-1).

QUESTION ID:9

Decimal reduction time of bacterial spores is 23 min at 121 ⁰C and the death kinetics follow first order. One liter medium containing 10^5 spores per mL was sterilized for 10 min at 121 ⁰C in a batch sterilizer. The number of spores in the medium after sterilization (assuming destruction of spores in heating and cooling period is negligible) will be ___________×10^7.

QUESTION ID:10

A bioreactor is scaled up based on equal impeller tip speed. Consider the following parameters for small and large bioreactors:

Assuming geometrical similarity and the bioreactors are operated in turbulent regime, what will be P2/P1?

QUESTION ID:11

An enzyme converts substrate A to product B. At a given liquid feed stream of flow rate 25 L.min-1 and feed substrate concentration of 2 mol.L-1, the volume of continuous stirred tank reactor needed for 95% conversion will be _____________ L.
Given the rate equation:

where ̶ rA is the rate of reaction in mol.L^-1.min^-1 and CA is the substrate concentration in mol.L^-1 Assumptions: Enzyme concentration is contant and does not undergo any deactivation during the reaction.

QUESTION ID:12

A protein is to be purified using ion-exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocity of mobile phase is given by:

where H is HETP (m) and u is linear liquid velocity of mobile phase (m.s^-1). The values of A, B and C are 3×10^-8 m2.s^-1, 3 s and 6×10^-5 m, respectively. The number of theoretical plates based on

minimum HETP for a column of 66 cm length will be ___________.

QUESTION ID:13

An enzyme is immobilized on the surface of a non-porous spherical particle of 2 mm diameter. The immobilized enzyme is suspended in a solution having bulk substrate concentration of 10 mM. The enzyme follows first order kinetics with rate constant 10 s^-1 and the external mass transfer coefficient is 1 cm.s^-1. Assume steady state condition wherein rate of enzyme reaction (mmol.L^-1.s^-1) at the surface is equal to mass transfer rate (mmol.L^-1.s^-1). The substrate concentration at the surface of the immobilized particle will be ________ mM.

QUESTION ID:14

 The initial conditions for this second order homogeneous differential equation are
y(0) = 1 and dy/dx  = 3 at x = 0
The value of y when x = 2 is ___________.

QUESTION ID:15

The value of determinant A given below is __________ .


QUESTION ID:16

Consider the equation

Given a = 4, b = 1 and c = 9, the positive value of S at which V is maximum, will be _______.

QUESTION ID:17

The volume of a sphere of diameter 1 unit is ________ than the volume of a cube of side 1 unit.

QUESTION ID:18

The unruly crowd demanded that the accused be _____________ without trial.

QUESTION ID:19

Choose the statement(s) where the underlined word is used correctly:
(i) A prone is a dried plum.
(ii) He was lying prone on the floor.
(iii) People who eat a lot of fat are prone to heart disease

QUESTION ID:20

Fact: If it rains, then the field is wet.
Read the following statements:
(i) It rains
(ii) The field is not wet
(iii) The field is wet
(iv) It did not rain
Which one of the options given below is NOT logically possible, based on the given fact?

QUESTION ID:21

A window is made up of a square portion and an equilateral triangle portion above it. The base of the triangular portion coincides with the upper side of the square. If the perimeter of the window is 6 m, the area of the window in m^2 is ___________.

QUESTION ID:22

Students taking an exam are divided into two groups, P and Q such that each group has the same number of students. The performance of each of the students in a test was evaluated out of 200 marks. It was observed that the mean of group P was 105, while that of group Q was 85. The standard deviation of group P was 25, while that of group Q was 5. Assuming that the marks were distributed on a normal distribution, which of the following statements will have the highest probability of being TRUE?

QUESTION ID:23

A smart city integrates all modes of transport, uses clean energy and promotes sustainable use of resources. It also uses technology to ensure safety and security of the city, something which critics argue, will lead to a surveillance state.
Which of the following can be logically inferred from the above paragraph?
(i) All smart cities encourage the formation of surveillance states.
(ii) Surveillance is an integral part of a smart city.
(iii) Sustainability and surveillance go hand in hand in a smart city.
(iv) There is a perception that smart cities promote surveillance.

QUESTION ID:24

Find the missing sequence in the letter series.
B, FH, LNP, _ _ _ _.

QUESTION ID:25

The binary operation □ is defined as a □ b = ab+(a+b), where a and b are any two real numbers. The value of the identity element of this operation, defined as the number x such that a □ x = a, for any a, is .

QUESTION ID:26

Which of the following curves represents the function  Here, x represents the abscissa and y represents the ordinate.