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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Eukaryotic transcription is carried out by

QUESTION ID:2

Acetylcholine released by the parasympathetic nerves has which one of the following functions in the heart pacemaker cells?

QUESTION ID:3

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion [a]: In multicellular organisms, cells of different lineages have different gene expression profiles.
Reason [r]: Alternative splicing is the only mechanism to generate protein diversity.

QUESTION ID:4

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion [a]: Chromosome mutations can change the structure of chromosomes.
Reason [r]: All chromosome mutations arise due to nondisjunction of chromosomes during mitosis or meiosis.

QUESTION ID:5

C-value paradox refers to

QUESTION ID:6

Which one of the following drugs is NOT an immune checkpoint inhibitor?

QUESTION ID:7

Dendritic cells are involved in cross-presentation of antigens. Which of the following protein(s) is(are) required for cross-presentation?
P. Basic leucine zipper ATF-like transcription factor 3 (BATF3)
Q. Membrane associated ring-CH-type finger 1 (MARCH-1)
R. Solute carrier family 10 member 1 (SLC10A1)
S. Class II-associated invariant chain peptide (CLIP)

QUESTION ID:8

Which one of the following is required for the development of B-cells in the bone marrow?

QUESTION ID:9

Which one of the following statements is TRUE about leghemoglobin?

QUESTION ID:10

The correct sequence of events during bacteriophage infection of a bacterial cell is

QUESTION ID:11

Intracellular proteins are targeted for proteolytic degradation in proteasomes upon conjugation with

QUESTION ID:12

In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?
P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase

QUESTION ID:13

In hybridoma technology, which one of the following enzymes is absent in the myeloma cells that are used for monoclonal antibody production?

QUESTION ID:14

Which of the following methods are used for detection of DNA and RNA, respectively?

QUESTION ID:15

Match the types of RNA in Group I with their corresponding function in Group II.


QUESTION ID:16

Which one of the following programs is used for finding distantly related (or remote) protein homologs?

QUESTION ID:17

Which one of the following is used for global alignment of two protein sequences?

QUESTION ID:18

Which one of the following methods CANNOT be used to determine the secondary structure content of a protein?

QUESTION ID:19

Which one of the following plant growth regulators facilitate adventitious root formation?

QUESTION ID:20

Fabry disease in humans is a X-linked disease. The probability (in percentage) for a phenotypically normal father and a carrier mother to have a son with Fabry disease is ____________.

QUESTION ID:21

The value of 

QUESTION ID:22

A series (S) is given as S = 1 + 3 + 5 + 7 + 9 + …… The sum of the first 50 terms of S is ____________.

QUESTION ID:23

Two fair six-sided dice are thrown. The probability of getting 12 as the product of the numbers on the dice (rounded off to two decimal places) is ____________.

QUESTION ID:24

If 73x = 216 , the value of 7-x (rounded off to three decimal places) is ____________.

QUESTION ID:25

The distance between the two points of intersection of  x2 + y = 7 and x + y = 7 (rounded off to two decimal places) is ____________.

QUESTION ID:26

Match the immune tolerance mechanisms in Group I with their respective outcomes in Group II.


QUESTION ID:27

Match the type of bacteria in Group I with their respective growth properties in Group II.


QUESTION ID:28

Match the virus in Group I with the type of genome it contains in Group II.


QUESTION ID:29

The event(s) that lead(s) to inactivation of tumor suppressor genes in cancer cells is(are)

QUESTION ID:30

Methylation of CpG islands near the promoter of a gene can inhibit transcription by

QUESTION ID:31

Which of the following statement(s) is(are) TRUE about induced pluripotent stem cells?

QUESTION ID:32

Which of the following statement(s) is(are) TRUE about fluoroquinolone drugs?

QUESTION ID:33

Which of the following is(are) plant protoplast fusogenic agent(s)?

QUESTION ID:34

Direct DNA transfer method(s) used for plant genetic engineering is(are)

QUESTION ID:35

Which of the following vector(s) is(are) used to clone a DNA fragment of size 220 kb?

QUESTION ID:36

The following reaction represents biomass synthesis from hexadecane
 where represents the biomass. The value of respiratory quotient (rounded off to two decimal places) is ___________.

QUESTION ID:37

Temperature of a reaction with an activation energy value of  15kcal .mol −1 is increased from 300 K to 310 K. If the value of the ideal gas constant (R) is 1.9872 cal .mol-1 .K−1 , the ratio of the reaction rate constants  (rounded off to two decimal places) is ___________.

QUESTION ID:38

E. coli is cultivated in a chemostat operated at a dilution rate of  0.2 h−1 . The values of biomass yield due to oxygen consumption and the steady state biomass concentration are  0.2 g.g−1 and 10 g.L−1 , respectively. The oxygen transfer rate (in  g.L -1.h −1 ) is ___________.

QUESTION ID:39

Aqueous two-phase extraction is used to recover α-amylase from a solution. A polypropylene glycol-dextran mixture is added and the solution separates into upper and lower phases. The partition coefficient is 4.0 and the ratio of upper to lower phase volume is 5.0. The enzyme recovery or yield (in percentage, rounded off to the nearest integer) is ___________.

QUESTION ID:40

E. coli cultivated at 298 K uptakes an uncharged compound (A) by passive diffusion. The intracellular and extracellular concentrations of A are 0.001 M and 0.1 M, respectively. If the value of the ideal gas constant R is 1.9872 cal.mol-1 .K − 1 , the free-energy change (in . kcalmol −1 ) for this passive diffusion of A (rounded off to two decimal places) is ___________.

QUESTION ID:41

If there are three unrooted trees for four protein sequences, the number of rooted trees for the same number of sequences is ___________.

QUESTION ID:42

The number of different possible ways of forming five intramolecular disulfide bonds with ten cysteine residues of a protein is ___________.

QUESTION ID:43

The following schematic diagram shows a chemostat with cell recycle

where F0 and Fr are the volumetric flow rates (in  Lh−1 ) of feed and recycle streams, respectively. X1, X0 and X are the cell concentrations (in  gL−1 ) in the reactor, recycle-stream and product-stream, respectively. If  = and X1 is 7.3 g.L -1  , the value of X (in  . gL−1 , rounded off to one decimal place) is ___________.

QUESTION ID:44

An enzyme (E) catalyzes the biochemical reaction A→B with kcat  equal to 500 s−1. If the initial reaction velocity ( 0 V ) is 10. s−1at the total enzyme
concentration [ Et] of 30 nM and substrate concentration [A] of 40 , the value of Km (in ) is ____________.

QUESTION ID:45

DNA sample collected from an unidentified bacterial species (Y) contains 13% of adenine. The G+C content (in percentage) of Y is ___________.

QUESTION ID:46

If 1000 bp of a double-helical DNA weighs 1 X10− 18 gm and distance between two bp is 0.34 nm , the total amount of DNA (in mg, rounded off to one decimal place) required to stretch from Earth to Moon (assuming the distance between Earth and Moon to be 3,74,000 km) is ___________.

QUESTION ID:47

A protein has three identical sites arranged at the vertices of an equilateral triangle. If one site is filled with a dye (donor), the measured quantum yield ( ) is 0.5. Filling one site with a donor dye and a second site with an acceptor dye results in of 0.25. The measured of one site filled with donor and the other two sites filled with acceptor dye (rounded off to three decimal places) is ___________.

QUESTION ID:48

If  the value of | A4 +3A2 −5A+ 6I | is ___________.

QUESTION ID:49

If   the value of f'(x ) at x = 0 is ___________.

QUESTION ID:50

If  f(2)  = 5 and ( (x) )(f ( x+ 1))  = 3  for all real values of x , the value of f(10)  is ___________

QUESTION ID:51

Ten playing cards numbered 1, 2, 3, …., 10 are placed face down on a table. One card is drawn at random, its number recorded, and then replaced face down. A card is drawn again at random. The probability that the number on the second draw is greater than the number on the first draw (rounded off to two decimal places) is ___________.

QUESTION ID:52

The values of the consistency index ‘ K ’ and the flow behavior index ‘ n ’ of a dilatant fluid are 0.415 (in CGS units) and 1.23, respectively. The value of the apparent viscosity (in g cm-1 s −1  ) of this fluid at a shear rate of 60 s− 1(rounded off to the nearest integer) is ___________.

QUESTION ID:53

An evaporator is insulated using glass wool material of 0.15 m thickness. The inner most surface and the outer surface of the insulation are at 700 0C and 80 0C  respectively. The mean thermal conductivity of the glass wool under these conditions is 0.29 W.m-1 .K − 1. The rate of heat loss (in W ) through  1.2 m2 of the evaporator wall surface (rounded off to the nearest integer) is __________.

QUESTION ID:54

A proportional controller is used to control the temperature of an autoclave from 60 0C to 130 0C . If the proportional band setting of the controller is 25%, the proportional gain value is __________.

QUESTION ID:55

A dNTP master-mix is prepared by combining 40 L of each 20 mM dNTP stock (dATP, dCTP, dGTP and dTTP). 4 L of this dNTP master-mix is added to a PCR mix and the final volume is adjusted to 50 L  . The concentration (in M) of total dNTPs in the PCR mix is __________.