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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Hybridoma technology is  used to produce

QUESTION ID:2

Ames test is used to determine

QUESTION ID:3

The bacteria known to be naturally competent for transformation of DNA is

QUESTION ID:4

Antibiotic resistance marker that CANNOT be used in a cloning vector in Gram negative bacteria is

QUESTION ID:5

Program used for essentially local similarity search is

QUESTION ID:6

Peptidyl transferase activity resides in

QUESTION ID:7

In transgenics, alterations in the sequence of nucleotide in genes are due to
P. Substitution
Q. Deletion
R. Insertion
S. Rearrangement

QUESTION ID:8

During transcription

QUESTION ID:9

Under stress conditions bacteria accumulate

QUESTION ID:10

An example for template independent DNA polymerase is

QUESTION ID:11

Which one of the following DOES NOT belong to the domain of Bacteria?

QUESTION ID:12

Interferon-beta is produced by

QUESTION ID:13

A culture of bacteria is infected with bacteriophage at a multiplicity of 0.3. The probability of a single cell infected with 3 phages is

QUESTION ID:14

A neonatally thymectomized mouse. immunized with protein antigen shows

QUESTION ID:15

Lymphocytes interact with foreign antigens in

QUESTION ID:16

Somatic cell gene transfer is used for

P. transgenic animal production

Q. transgenic diploid cell production

R. in-vitro fertilization

s. classical breeding of farm animals

QUESTION ID:17

Accession number is a unique identification assigned to a

QUESTION ID:18

Expressed Sequence Tag is defined as

QUESTION ID:19

In a chemostat operating under steady state a bacterial culture can be grown at dilution rate higher than maximum growth rate by

QUESTION ID:20

During lactic acid fermentation. net yield of ATP and NADH per mole of glucose is

QUESTION ID:21

Identify tile enzyme that catalyzes the following reaction alpha -Ketoglutarate

QUESTION ID:22

The degree of inhibition for an enzyme catalyzed reaction at a particular inhibition concentration is independent of initial substrate concentration. The inhibition follows

QUESTION ID:23

Oxidation reduction reactions with positive standard redox potential   have

QUESTION ID:24

Nuclease-hypersensitive sites in the chromosomes arc sites that appear to be

QUESTION ID:25

The formation of peptide cross-links between adjacent glycan chain in cell wall synthesis is called

QUESTION ID:26

Determine the correctness or otherwise  given of the following Assertion (a) and the Reason (r)
Assertion: Somatic embryogenesis in plants is a two step process comprising of embryo initiation followed hy embryo production
Reason: Embryo initiation is independent of the presence of -dichlorophenoxyacetic acid whereas embryo production requires a high concentration of 2. 4- dichlorophenoxyacetic acid.

QUESTION ID:27

An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (n) of I.2 . The value of  of  being greater than I.0 could he apparently due to

QUESTION ID:28

A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 cm and length (I)= 20 cm was fixed with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 10^5anchorage dependent cells per cm^2, the increase in the surface area after fitting the spiral film and the additional number of cells that can be grown respectively are

QUESTION ID:29

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)
Assertion: MTT assay io used to determine cell viability based on the principle of colour formation by DNA fragmentation.
Reason: MTT assay is used to determine cell viability based on the colour development by convening tetrazolium soluble salt to insoluble salt.

QUESTION ID:30

Match the following antibiotics in Group I with their mode of action in Group II


QUESTION ID:31

Match the chemicals in Group I with the possible type/class in Group II


QUESTION ID:32

Match Group I with Group II


QUESTION ID:33

Match the promoters listed in Group I with the tissues listed in Group II


QUESTION ID:34

Consider the following statements.
I. T4 DNA ligase can catalyze blunt end ligation more efficiently than E. coli DNA ligase
II. The ligation efficiency ofT4 DNA ligase can be increased with PEG and ficoll.

QUESTION ID:35

The turnover numbers for the enzymes. E1 and E2 are 150 s^-1 and 15 s-1 respectively. This means

QUESTION ID:36

Match the items in Group I with Group II


QUESTION ID:37

Match Group I with Group II


QUESTION ID:38

A mutant G alpha protein with increased GTPase activity would

QUESTION ID:39

Dizygotic twins are conn<:<: ted to a single placenta during their embryonic development .these twins

QUESTION ID:40

The dissociation constant K. for ligand binding to the receptor is 10^7 M. The concentration of ligand required for occupying 10% of receptors is

QUESTION ID:41

Receptor R is over expressed in CHO cells and analysed for expression. 6 x 10^7 cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm. the average number of receptors R per cell is {assume complete saturation of receptors with ligand and one ligand binds to one receptor)

QUESTION ID:42

A cell has five molecules of a rare mRNA. Each cell contains 4 x 10^5 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant eDNA of the rare mRNA. after making eDNA library from such cell?

QUESTION ID:43

Match the products in Group I with the microbial cultures in Group II used for their industrial production

QUESTION ID:44

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)
Assertion·. Cytoplasmic male sterility (cms) is invariably due to defect(s) in mitochondrial function_
Reason: ems can be overcome by pollinating a fertility restoring (Rf plant with pollen from a non cms plant.

QUESTION ID:45

Thermal death of microorganisms in the liquid medium follows first order kinetics. If the initial cell concentration in the fermentation medium is 10^8 cells / ml and the final acceptable contamination level is 10^3 cells, for how long should lm^3 medium be treated at temperature of 120° (thermal deactivation rate constant = 0.23 I min) to achieve acceptable load?

QUESTION ID:46

True breeding Drosophila flies with curved wings and dark bodies were mated with true breeding short wings and tan body Drosophila. The F 1 progeny was observed to be with curved wings and tan body. The F 1 progeny was again allowed to breed and produced flies of the following phenotype, 45 curved wings tan body, 1.5 short wings tan body, 16 curved wings dark body and, 6 short wings dark body.
The mode of inheritance is

QUESTION ID:47

Match Group I with Group II


QUESTION ID:48

A culture of Rhizobium is grown in a chemostat (100m^3 bioreactor). The feed contains 12 g / L sucrose, K, lor the organism,. 0 2 g / I and  = 0.3 h^-1
The flow rate required to result in steady state concentration of sucrose as 1.5 g / L in the bioreactor will be

QUESTION ID:49

A culture of Rhizobium is grown in a chemostat (100m^3 bioreactor). The feed contains 12 g / L sucrose, K, lor the organism,. 0 2 g / I and  = 0.3 h^-1

If Y = 0.4 g / g for the above culture and steady state cell concentration in the bioreactor is 4 g / L the resulting substrate concentration will be

QUESTION ID:50

The width of the lipid bilayer membrane is 30 A. It is permeated by a protein which is a right handed alpha-helix.
Tile number of a-helical turns permeating the membrane is

QUESTION ID:51

The width of the lipid bilayer membrane is 30 A. It is permeated by a protein which is a right handed alpha-helix.

The number of amino acid residues present in the protein is

QUESTION ID:52

The standard redox potential values for two half-reaction are given below. The value for Faraday's constant is 96.48 kJ v^-1 mol^-1 and Gas constant R is 8.31 J K^-1 mol^-1.
The △G° for the oxidation of NADH by FAD is

QUESTION ID:53

The standard redox potential values for two half-reaction are given below. The value for Faraday's constant is 96.48 kJ v^-1 mol^-1 and Gas constant R is 8.31 J K^-1 mol^-1.

The value of, given Keq as 1.7. at23°C will be

QUESTION ID:54

During bioconversion of sucrose to citric acid by Aspergillus niger final samples of 6 batches of fermentation broth were analyzed for citric acid content The result (in g/L) were found to be 47.3, 52.2, 49.2, 52.4, 49.1 and 46.3.
The mean value of acid concentration will be

QUESTION ID:55

During bioconversion of sucrose to citric acid by Aspergillus niger final samples of 6 batches of fermentation broth were analyzed for citric acid content The result (in g/L) were found to be 47.3, 52.2, 49.2, 52.4, 49.1 and 46.3.

The standard deviation for the above results is

QUESTION ID:56

Which of the following options is the closest in meaning to the word below:
circuitous

QUESTION ID:57

The question below consists of a pair of related words followed by four pair-s of words. Select the pair that best expresses the relation in the original pair.
Unemployed :Worker

QUESTION ID:58

Choose the most appropriate word from the options given below to complete the following sentence:
If we manage too ------- our natural resources, we would leave a better planet for our children.

QUESTION ID:59

Choose the most appropriate word from the options given below to complete the following sentence:
His rather casual remarks on politics ______ his lack of seriousness about the subject.

QUESTION ID:60

25 persons are in a room. 15 of them play hockey, 17 of them play football and 10 of them play both hockey and football. Then the number of persons playing neither hockey nor football is:

QUESTION ID:61

Modem warfare bas changed from large scale clashes of armies to suppression of civilian populations. Chemical agents that do their work silently appear to be suited to such warfare; and regretfully, there exist people In military establishments who think that chemical agents are useful tools for their cause.
Which of the following statements best sums up the meaning of the above passage:

QUESTION ID:62

If 137 + 276 = 435 how much is 731 + 672 ?

QUESTION ID:63

5 skilled workers can build a wall in 20 days; 8 semi-skilled workers can build a wall 25 days; 10 unskilled workers can build n wall in 30 days. If a team has 2 skilled, 6 semi-skilled and 5 unskilled workers. how long will it take to build the wall?

QUESTION ID:64

Given digits 2, 2, 3, 3, 3, 4, 4, 4, 4 how many distinct 4 digit numbers greater than 3000 can be formed?

QUESTION ID:65

Hari (H), Gita (G), lrfan (I) and Sarita (S) arc siblings (i.e brothers and sisters). All were born on first January. The age difference between any two successive siblings (that is born one after another) is less than 3 years. Given the following facts:
i. Hari · s age + Gita 's age > lrfan' s age + Sarita' s age.
ii. The age difference between Gila and Sarita is I year. However. Gita is not the oldest and Sarita is not the youngest.
iii. There are no twins.
In what order were they born (oldest first)?