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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Embryonic stem cells are derived from

QUESTION ID:2

Members  of  the  antibody   protein   family   that  have  common   structural   features   are ollectively known as

QUESTION ID:3

Apoptosis is characterized by

QUESTION ID:4

Yeast artificial chromosomes (YAC's) are used for cloning

QUESTION ID:5

The product commercially produced by animal cell culture is

QUESTION ID:6

An alternative to glycolysis pathway is

QUESTION ID:7

A cell in G1 of interphase has 12 chromosomes. How many chromatids will be found per cell during metaphase II of meiosis?

QUESTION ID:8

Diploid Drosophila has eight chromosomes. Which one of the following terms should NOT be used to describe Drosophila with sixteen numbers of chromosomes?

QUESTION ID:9

Hydrated synthetic seeds which are produced by ion exchange reaction involve mixing the somatic embryos in a solution of

QUESTION ID:10

Shoot organogenesis by tissue culture results into

QUESTION ID:11

'Hairy roots' induced in vitro by the infection of Agrobacterium rhizogenes, are characterized by
P. a high degree of lateral branching
Q. genetic instability of culture
R. an absence of geotropism
S. poor biomass production

QUESTION ID:12

In balanced growth phase of a cell
P. all components of a cell grow at the same rate
Q. specific growth determined by cell number or cell mass would be the same
R. the growth rate is independent of substrate concentration
S. the growth rate decreases with decreasing substrate concentration

QUESTION ID:13

In N-linked glycosylation, the oligosaccharide chain is attached to protein by

QUESTION ID:14

Restriction endonucleases which recognize and cut same recognition sequences are known as

QUESTION ID:15

Substrate consumption in lag phase of microbial growth is primarily used for
P. tum over of the cell material
Q. maintenance of intracellular pH
R. motility
S. increase in cell number

QUESTION ID:16

Wash out (as defined by D=!) of a continuous stirred tank fermenter is characterized by (X=biomass, S=substrate concentration in bioreactor, S0=substrate concentration in feed, P=product concentration in bioreactor)

QUESTION ID:17

The study of evolutionary relationships is known as

QUESTION ID:18

The lipopolysaccharides present in bacterial cell wall has lipid A which is connected to

QUESTION ID:19

Molecular chaperones are class of proteins that facilitate

QUESTION ID:20

Gas vacuoles are present in

QUESTION ID:21

In ABO blood group system, antigenic determinants are

QUESTION ID:22

The most widely used program for multiple sequence alignment is

QUESTION ID:23

Diphtheria toxin, tetracycline and streptomycin inhibit

QUESTION ID:24

The polymorphic domains for Class II MHC proteins are

QUESTION ID:25

The protein in eukaryotes which is subjected to degradation undergoes

QUESTION ID:26

Match the viruses in Group I with their host cell receptors in Group II.

QUESTION ID:27

Match the microbial growth characteristics in Group I with the corresponding features in Group II.


QUESTION ID:28

Match the items in Group I with Group II.


QUESTION ID:29

Match the products in Group I with their respective organisms in Group II.


QUESTION ID:30

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r).
Assertion: lgM is found in serum as a pentameric protein consisting of five lgM monomers.
Reason: The pentameric form of lgM is due to cross-linking of lgM monomers via peptide bond.

QUESTION ID:31

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r).
Assertion: N-methyl-N' -nitro-N-nitrosoguanidine (NTG) is an effective chemical mutagen.
Reason: Mutations induced by NTG mainly are the GC ~ AT transitions.

QUESTION ID:32

Determine the correctness of the following statements
I. Enhancer sequences are those DNA sequences that are involved in increasing the rate of DNA replication.
II. Enhancer sequences work by binding with eukaryotic gene activator factors.

QUESTION ID:33

In a well aerated and agitated microbial culture, the 'supply' of oxygen is equal to 'demand' (uptake) of the growing culture. The KLa for such a system will be (KLa = volumetric mass transfer coefficient, c* = dissolved oxygen concentration in liquid in equilibrium with gaseous oxygen, C = instantaneous value of dissolved oxygen concentration, 'r' = specific oxygen uptake rate per unit weight of cells, X = dry weight of the cells per unit volume)

QUESTION ID:34

Structured William's model
P. can describe the changes in intracellular components of the cell during growth
Q. can not describe the death phase of the cells
R. can describe the variation of size of cells in the different phases of growth
S. can not describe the lag period of growth
Which one of the following is CORRECT?

QUESTION ID:35

Match items in Group I with Group II.


QUESTION ID:36

Match items in Group I with Group II.


QUESTION ID:37

Determine the corr-ectness or otherwise of the following Assertion (a) and the Reason (r)
Assertion: The elucidation of ribosome structure helps in the development of new generation drugs.
Reason: The high resolution of macromolecular structure has enabled in structure-based drug design.

QUESTION ID:38

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)
Assertion : A very low amount of inhibitor can act as an activator for allosteric enzymes.
Reason: Alloste1ic enzymes follow Michaelis-Menten kinetics.

QUESTION ID:39

Match the terms in Group I with their associated functions in Group II.


QUESTION ID:40

Protein-protein interactions are studied by
P. DNA foot printing
Q. Yeast two hybrid system
R. Ligase chain reaction
S. Mass spectrometry

QUESTION ID:41

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r)
Assertion : Isopropylthiogalactoside (IPTG) is a gratuitous inducer of lactose operon.
Reason: Gratuitous inducers are chemical analogs which behave like natural inducer but they do not serve as substrate for the enzymes that are subsequently synthesized.

QUESTION ID:42

Determine the con·ectness or otherwise of the following Assertion (a) and the Reason (r)
Assertion : In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously.
Reason : Synchronous culture could be obtained by starving cells for essential nut1ient components.

QUESTION ID:43

Which of the following characteristics with respect to bacterial DNA polymerase III are TRUE?
P. Initiation of chain synthesis
Q. 5'- 3' polymerization
R. 3'- 5' exonuclease activity
S. 5'- 3' exonuclease activity

QUESTION ID:44

Maximum specific growth rate (!lmax) of a microorganism is calculated by taking the (ln=loge, X=biomass, t =time)

QUESTION ID:45

Identify the CORRECT statements
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction
Q. S1 nuclease can cleave the DNA strand of a DNA-RNA hybrid
R. T 4 polynucleotide kinase is used for labeling 3' end of DNA
S. Baculovirus (Autographa californica) can be used as an insect expression vector

QUESTION ID:46

Value of the determinant mentioned below is


QUESTION ID:47

HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following
I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine.
II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.

QUESTION ID:48

Red-green colour blindness is inherited as a recessive X-linked trait.
What will be the probability of having the colour-blind son to a woman with phenotypically normal parents and a colour-blind brother, and married to a normal man? (Assume that she has no previous children)

QUESTION ID:49

Red-green colour blindness is inherited as a recessive X-linked trait.

What will be the probability of having the colour-blind daughter to a phenotypically normal woman, who already had one colour-blind son, and is married to a colour-blind man?

QUESTION ID:50

A microorganism grows in a continuous 'chemostat' culture of 60 m^3 working volume with sucrose as the growth limiting nutrient at dilution rate, D = 0.55 h^- 1 . The steady state biomass concentration is 4.5 Kg dry biomass m^·3 and the residual sucrose concentration is 2.0 Kg m^·3. The sucrose concentration in the incoming feed medium is 10.0 Kg m·^3
What would be the yield YXJs (Kg biomass/Kg substrate)?

QUESTION ID:51

A microorganism grows in a continuous 'chemostat' culture of 60 m^3 working volume with sucrose as the growth limiting nutrient at dilution rate, D = 0.55 h^- 1 . The steady state biomass concentration is 4.5 Kg dry biomass m^·3 and the residual sucrose concentration is 2.0 Kg m^·3. The sucrose concentration in the incoming feed medium is 10.0 Kg m·^3

What would be the sucrose concentration in the input feed for the output to be 45 Kg biomass h^- 1?

QUESTION ID:52

The abdomen length (in millimeters) was measured in 15 male fruit flies, and the following data were obtained: 1.9, 2.4, 2.1, 2.0, 2.2, 2.4, 1.7, 1.8, 2.0, 2.0, 2.3, 2.1, 1.6, 2.3 and 2.2.
Vmiance (Vx) for this population of fruit flies as calculated from the above data shall be

QUESTION ID:53

The abdomen length (in millimeters) was measured in 15 male fruit flies, and the following data were obtained: 1.9, 2.4, 2.1, 2.0, 2.2, 2.4, 1.7, 1.8, 2.0, 2.0, 2.3, 2.1, 1.6, 2.3 and 2.2.
The value of standard deviation (SD) will be

QUESTION ID:54

A 200  of polymerase chain reaction has 100 template DNA molecules and the reaction was performed for 10 cycles.
How many molecules of amplicons will be generated?

QUESTION ID:55

A 200  of polymerase chain reaction has 100 template DNA molecules and the reaction was performed for 10 cycles.

How many molecules of amplicons will be present in 0.1 fll of reaction?

QUESTION ID:56

Which of the following options is the closest in the meaning to the word below:
Inexplicable

QUESTION ID:57

Choose the word from the options given below that is most nearly opposite in meaning to the given word:
Amalgamate

QUESTION ID:58

Choose the most appropriate word from the options given below to complete the following sentence.
If you are trying to make a strong impression on your audience, you cannot do so by being understated, tentative or _____ _

QUESTION ID:59

Choose the most appropriate word(s) from the options given below to complete the following sentence.
I contemplated ____ Singapore for my vacation but decided against it.

QUESTION ID:60

If Log (P) = (l/2)Log (Q) = (1/3) Log (R), then which of the following options is TRUE?

QUESTION ID:61

Few school curricula include a unit on how to deal with bereavement and grief, and yet all students at some point in their lives suffer from losses through death and parting.
Based on the above passage which topic would not be included in a unit on bereavement?

QUESTION ID:62

A container originally contains 10 litres of pure spirit. From this container 1 litre of spirit is replaced with 1 litre of water. Subsequently, 1 litre of the mixture is again replaced with 1 litre of water and this process is repeated one more time. How much spirit is now left in the container?

QUESTION ID:63

A transporter receives the same number of orders each day. Cunently, he has some pending orders (backlog) to be shipped. If he uses 7 trucks, then at the end of the 4th day he can clear all the orders. Alternatively, if he uses only 3 trucks, then all the orders are cleared at the end of the 4th day. What is the minimum number of trucks required so that there will be no pending order at the end of the 5th day?

QUESTION ID:64

The variable cost (V) of manufacturing a product varies according to the equation V= 4q, where q is the quantity produced. The fixed cost (F) of production of same product reduces with q according to the equation F = 100/q. How many units should be produced to minimize the total cost (V+F)?

QUESTION ID:65

P, Q, RandS are four types of dangerous microbes recently found in a human habitat. The area of each circle with its diameter printed in brackets represents the growth of a single microbe surviving human immunity system within 24 hours of entering the body. The danger to human beings vaties proportionately with the toxicity, potency and growth attributed to a microbe shown in the figure below:

A pharmaceutical company is contemplating the development of a vaccine against the most dangerous microbe. Which microbe should the company target in its first attempt?