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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand by

QUESTION ID:2

The basis for blue-white screening with pUC vectors is

QUESTION ID:3

Idiotypic determinants of an antibody are associated with the

QUESTION ID:4

I dentification of blood groups involves

QUESTION ID:5

B -lymphocytes originate from the bone marrow whereas T-lymphocytes originate from

QUESTION ID:6

A humanized antibody is one in which the

QUESTION ID:7

Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because

QUESTION ID:8

Nude mice refers to

QUESTION ID:9

Heat inactivation of serum is done to inactivate

QUESTION ID:10

Choose the correct signal transduction pathway.

QUESTION ID:11

A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with

QUESTION ID:12

A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?

QUESTION ID:13

Protein-DNA interactions in vivo can be studied by

QUESTION ID:14

The direction of shell coiling in the snail Limnaea peregra is a classic example of

QUESTION ID:15

During photorespiration under low CO2 and high O2 levels, O2 reacts with ribulose 1,5- bisphosphate to yield

QUESTION ID:16

Which one of the following is NOT a protoplast fusion inducing agent?

QUESTION ID:17

The activity of an enzyme is expressed in International Units (IU). However, the S.I. unit for enzyme activity is Katal. One Katal is

QUESTION ID:18

Identify the statement that is NOT applicable to an enzyme catalyzed reaction.

QUESTION ID:19

An example of a derived protein structure database is

QUESTION ID:20

An example of a program for constructing a phylogenetic tree is

QUESTION ID:21

Synteny refers to

QUESTION ID:22

W hile searching a database for similar sequences, E value does NOT depend on the

QUESTION ID:23

In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen with

QUESTION ID:24

The molarity of water in a water : ethanol mixture (15 : 85, v/v) is approximately

QUESTION ID:25

The helix content of a protein can be determined using

QUESTION ID:26

Which one of the following DNA sequences carries an invert repeat?

QUESTION ID:27

In zinc finger proteins, the amino acid residues that coordinate zinc are

QUESTION ID:28

Match the entries in Group I with those in Group II.


QUESTION ID:29

In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 20 gl^-1 (DCW), the specific growth rate is 0.4 h^-1 and substrate uptake rate (v) is 16 gl^-1h^-1. The cell yield coefficient Yx/s will be

QUESTION ID:30

A single base pair of DNA weighs 1.1 × 10^-21 grams. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 of purified DNA?

QUESTION ID:31

Match the terms in Group I with the ploidy in Group II.


QUESTION ID:32

What is the rank of the following matrix?


QUESTION ID:33

Match the products in Group I with the applications in Group II.


QUESTION ID:34

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: The production of secondary metabolites in plant cell cultures is enhanced by the addition of elicitors.
Reason: Elicitors induce the expression of enzymes responsible for the biosynthesis of secondary metabolites.

QUESTION ID:35

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: Plants convert fatty acids into glucose.
Reason: Plants have peroxisomes.

QUESTION ID:36

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: In direct somatic embryogenesis, embryos are developed without going through callus formation.
Reason: This is possible due to the presence of pre-embryonically determined cells.

QUESTION ID:37

Match the entries in Group I with the process parameters in Group II.


QUESTION ID:38

Match the downstream processes in Group I with the products in Group II


QUESTION ID:39

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: Cell mass yield of a methylotrophic yeast is more on methanol compared to glucose.
Reason: Methanol has a greater degree of reductance compared to glucose.

QUESTION ID:40

Adisease is inherited by a child with a probability of 1/4. In a family with two children, the probability that exactly one sibling is affected by this disease is

QUESTION ID:41

Match the organisms in Group I with the entries in Group II.


QUESTION ID:42

Match the entries in Group I with the methods of sterilization in Group II.


QUESTION ID:43

Match the high energy compounds in Group I with the biosynthetic pathways for the molecules in Group II.

QUESTION ID:44

Match the vitamins in Group I with the processes/reactions in Group II.


QUESTION ID:45

Consider the data set 14, 18, 14, 14, 10, 29, 33, 31, 25. If you add 20 to each of the values, then

QUESTION ID:46

An enzymatic reaction is described by the following rate expression

Which one of the following curves represents this expression?


QUESTION ID:47

A bacterial culture (200 containing 1.8 × 10^9 cells) was treated with an antibiotic Z (50 per ml) for 4 h at 37˚C. After this treatment, the culture was divided into two equal aliquots.
Set A: 100 was plated on Luria agar.
Set B: 100 was centrifuged, the cell pellet washed and plated on Luria agar.
After incubating these two plates for 24 h at 37˚C, Set A plate showed no colonies, whereas the Set B plate showed 0.9 × 10^9 cells. This experiment showed that the antibiotic Z is

QUESTION ID:48

In a muscle, the extracellular and intracellular concentrations of Na^+ are 150 mM and 12 mM, and those of K^+ are 2.7 mM and 140 mM, respectively. Assume that the temperature is 25^oC and that the membrane potential is -60 mV, with the interior more negatively charged than the exterior. (R = 8.314 J mol^-1 K^-1; F = 96.45 kJ mol^-1 V^-1)
The free energy change for the transport of three Na^+ out of the cell is

QUESTION ID:49

In a muscle, the extracellular and intracellular concentrations of Na^+ are 150 mM and 12 mM, and those of K^+ are 2.7 mM and 140 mM, respectively. Assume that the temperature is 25^oC and that the membrane potential is -60 mV, with the interior more negatively charged than the exterior. (R = 8.314 J mol^-1 K^-1; F = 96.45 kJ mol^-1 V^-1)

The free energy change for the transport of two K^+ into the cell is

QUESTION ID:50

The purification data for an enzyme is given below:

The fold purification for each step is

QUESTION ID:51

The purification data for an enzyme is given below:

The yield (%) for each step is

QUESTION ID:52

An E. coli cell of volume 10^-12 cm^3 contains 60 molecules of lac-repressor. The repressor has a binding affinity (Kd) of 10^-8 M and 10^-9 M with and without lactose respectively, in the medium. The molar concentration of the repressor in the cell is

QUESTION ID:53

An E. coli cell of volume 10^-12 cm^3 contains 60 molecules of lac-repressor. The repressor has a binding affinity (Kd) of 10^-8 M and 10^-9 M with and without lactose respectively, in the medium. Therefore the lac-operon is

QUESTION ID:54

Beta-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume 100 liters and a voidage of 0.55. The K'm and V'max for the immobilized enzyme are 0.72 gl^-1 and 18 gl^-1h^-1, respectively. The lactose concentration in the field stream is 20 gl^-1, and a fractional conversion of 0.90 is desired. Diffusional limitations may be ignored.
The residence time required for the steady state reactor operation will be

QUESTION ID:55

Beta-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume 100 liters and a voidage of 0.55. The K'm and V'max for the immobilized enzyme are 0.72 gl^-1 and 18 gl^-1h^-1, respectively. The lactose concentration in the field stream is 20 gl^-1, and a fractional conversion of 0.90 is desired. Diffusional limitations may be ignored.

The feed flow rate required for the above bioconversion will be

QUESTION ID:56

The cost function for a product in a firm is given by 5q^2, where q is the amount of production. The firm can sell the product at a market price of 50 per unit. The number of units to be produced by the firm such that the profit is maximized is

QUESTION ID:57

Choose the most appropriate alternative from the options given below to complete the following sentence:
Suresh’s dog is the one ––––––––– was hurt in the stampede.

QUESTION ID:58

hoose the grammatically INCORRECT sentence:

QUESTION ID:59

Which one of the following options is the closest in meaning to the word given below?
Mitigate

QUESTION ID:60

Choose the most appropriate alternative from the options given below to complete the following sentence:
Despite several ––––––––– the mission succeeded in its attempt to resolve the conflict.

QUESTION ID:61

Wanted Temporary, Part-time persons for the post of Field Interviewer to conduct personal interviews to collect and collate economic data. Requirements: High School-pass, must be available for Day, Evening and Saturday work. Transportation paid, expenses reimbursed.
Which one of the following is the best inference from the above advertisement?

QUESTION ID:62

Given the sequence of terms, AD CG FK JP, the next term is

QUESTION ID:63

Which of the following assertions are CORRECT?
P: Adding 7 to each entry in a list adds 7 to the mean of the list
Q: Adding 7 to each entry in a list adds 7 to the standard deviation of the list
R: Doubling each entry in a list doubles the mean of the list
S: Doubling each entry in a list leaves the standard deviation of the list unchanged

QUESTION ID:64

An automobile plant contracted to buy shock absorbers from two suppliers X and Y. X supplies 60% and Y supplies 40% of the shock absorbers. All shock absorbers are subjected to a quality test. The ones that pass the quality test are considered reliable. Of X’s shock absorbers, 96% are reliable. Of Y’s shock absorbers, 72% are reliable.
The probability that a randomly chosen shock absorber, which is found to be reliable, is made by Y is

QUESTION ID:65

A political party orders an arch for the entrance to the ground in which the annual convention is being held. The profile of the arch follows the equation y = 2x – 0.1x^2 where y is the height of the arch in meters. The maximum possible height of the arch is