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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Under alkaline conditions, DNA is more stable than RNA because 

QUESTION ID:2

Which one of the following modifications is common toboth protein and DNA? 

QUESTION ID:3

Protein A, which has strong affinity to Fc region of immunoglobulin, is extracted from 

QUESTION ID:4

The first humanized monoclonal antibody approved for the treatment of breast canceris

QUESTION ID:5

Which one of the following aminoacids in proteins does NOT undergo phosphorylation?

QUESTION ID:6

The role of an adjuvant isto

QUESTION ID:7

Endogenous antigens are presented on to the cell surface along with

QUESTION ID:8

Human genome sequencing project involved the construction of genomic library in 

QUESTION ID:9

The nucleotide analogue used in DNA sequencing by chain termination method is

QUESTION ID:10

In nature, the horizontal gene transfer across bacteria is mediated by

QUESTION ID:11

Phylum proteobacteria is subdivided into  proteobacteria based on

QUESTION ID:12

Which one of the following is an ABC transporter? 

QUESTION ID:13

The catalytic efficiency for an enzyme is definedas


QUESTION ID:14

Of the two diploid species, species I has 36 chromosomes and species II has 28 chromosomes. How  many chromosomes would be found in an allotriploid individual? 

QUESTION ID:15

The RNA primer synthesized during the replication process in bacteria is removed by 

QUESTION ID:16

The suitable substitution matrix to align closely related sequences is 

QUESTION ID:17

QUESTION ID:18

QUESTION ID:19

Hypophosphatemia is manifested by an X-linked dominant allele.What proportion of the offsprings  from a normal male and an affected heterozygous female will manifest the disease? 

QUESTION ID:20

One of theeigen values of 

QUESTION ID:21

A callus of 5 g dry weight was inoculated on semi-solid medium for growth. The dry weight of the callus was found to increase by 1.5 fold after 10 days of inoculation. The growth index of the culture is _________

QUESTION ID:22

A batch bioreactor is to be scaled up from 10 to 10,000 liters. The diameter of the large bioreactor is 10 times that of the small bioreactor. The agitator speed in the small bioreactor is 450 rpm. Determine the agitator speed (rpm) of the large bioreactor with same impeller tip speed as that of the small bioreactor. _________

QUESTION ID:23

Calculate the percentage sequence identity for the pairwise alignment given below. _______
H E L L O –
Y E L L O W

QUESTION ID:24

In a batch culture, the specific rate of substrate utilization is 0.25 g (g cell mass)-1 h-1 and specific rate of product formation is 0.215 g (g cell mass)-1 h-1. Calculate the yield of product from the substrate(Yp/s). ___________

QUESTION ID:25

A chemostat is operated at a dilution rate of 0.6 h-1. At steady state, the biomass concentration in the exit stream was found to be 30 g l-1. The biomass productivity (g l-1h-1) after 3h of steady state
operation will be ___________

QUESTION ID:26

Match the commercial microbial sources in Group I with the products in Group II.

QUESTION ID:27

Match the entries in the Group I with the elution conditions in Group II.

QUESTION ID:28

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation.
Reason: Immobilization protects the plant cells from shear forces in the bioreactor.

QUESTION ID:29

Match the cell structures in Group I with the organismsin Group II.


QUESTION ID:30

Match the antibiotic sin Group I with the targets in Group II.


QUESTION ID:31

In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to
P. crown gall disease in plants
Q. hairy root disease in plants
R. transfer of T-DNA into the plant chromosome
S. transfer of Ri-plasmid into the plant cell

QUESTION ID:32

Match the entries in Group I with the enzymes in Group II.


QUESTION ID:33

Match the herbicides in Group I with the target enzyme sin Group II.


QUESTION ID:34

The activity of an enzyme was measured by varying the concentration of the substrate (S) in the presence of three different concentrations of inhibitor (I) 0, 2 and 4 mM. The double reciprocal plot given below suggests  that the inhibitor (I) exhibits

QUESTION ID:35

Match the entries in Group I with the entries in Group II.


QUESTION ID:36

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: UPGMA method produces ultra metric tree.
Reason: Sequence alignment is converted into evolutionary distances in UPGMA method.

QUESTION ID:37

Evaluate 

QUESTION ID:38

The Laplace transform of f(t) = 2t+ 6 is 

QUESTION ID:39

Match the entries in the Group I with the entries in Group II.


QUESTION ID:40

The solution of the following set of equations is

QUESTION ID:41


QUESTION ID:42

A complete restriction digestion of a circular plasmid (5000bp) was carried out with Hind III, Bam HI and Eco RI individually. Restriction digestion yielded following fragments.
Plasmid + Hind III→ 1200bp and3800bp
Plasmid + Bam HI→5000bp
Plasmid + Eco RI→ 2500bp
The number of sites for Eco RI, Bam HI and Hind III present on this plasmid are

QUESTION ID:43

The total number of fragments generated by the complete and sequential cleavage of the polypeptide given below by Trypsin followed by CNBr is _________
Phe-Trp-Met-Gly-Ala-Lys-Leu-Pro-Met-Asp-Gly-Arg-Cys-Ala-Gln

QUESTION ID:44

In a genetic study, 80 people were found to have alleles for polydactyly. Only 36 of them were polydactylous. What is the extent of penetrance percentage? ________

QUESTION ID:45

One percent of the cars manufactured by a company are defective. What is the probability ( upto four decimals) that more than two cars are defective, if 100 cars are produced? __________

QUESTION ID:46

The maximum cell concentration (g l^-1) expected in a bioreactor with initial cell concentration of 1.75 g l^-1 and an initial glucose concentration of 125 g l^-1 is (Yx/s = 0.6 g cell/g substrate

QUESTION ID:47

A fed batch culture was operated with intermittent addition of glucose solution at a flow rate of 200 ml h^-1. The values of Ks, m and D are 0.3 g l^-1, 0.4 h-1 and 0.1 h^-1, respectively. Determine the
concentration of growth limiting substrate (gl^-1) in the reactor at quasi-steady state. ____________

QUESTION ID:48

A solution was prepared by dissolving 100 mg of protein X in 100 ml of water. Molecular weight of protein X is 15,000 Da; Avogadro’s number = 6.022x 10^23.
Calculate the molarity(M) of the resulting solution.

QUESTION ID:49

A solution was prepared by dissolving 100 mg of protein X in 100 ml of water. Molecular weight of protein X is 15,000 Da; Avogadro’s number = 6.022x 10^23.

The number of moleculespresent in this solution is

QUESTION ID:50

The binding efficiency of three different receptorsR1, R2 and R3 were tested against a ligand using equilibrium dialysis, with a constant concentration of receptor and varying concentrations of ligand. The Scatchard plot of receptor titration with different concentration of ligand is given below (ris moles of bound ligand per moles of receptor and c is concentration of free ligand)
The number of ligand binding sites present on receptors R1 and R3, respectively are

QUESTION ID:51

The binding efficiency of three different receptorsR1, R2 and R3 were tested against a ligand using equilibrium dialysis, with a constant concentration of receptor and varying concentrations of ligand. The Scatchard plot of receptor titration with different concentration of ligand is given below (ris moles of bound ligand per moles of receptor and c is concentration of free ligand)

Which one of the receptors has thehighestaffinity for the ligand?

QUESTION ID:52

A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x10^3kb) genomic library.
The minimum number of independent recombinant clones required to represent this fragment in genomic library are

QUESTION ID:53

A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x10^3kb) genomic library.

The number of clones to represent this fragment in genomic library with a probability of 95% are

QUESTION ID:54

During sterilization of a fermentation medium in a given bioreactor heating= 12.56, cooling = 7.48 and the total value of required for whole sterilization process is 52, where is the design criteria.
What is the value of holding?

QUESTION ID:55

During sterilization of a fermentation medium in a given bioreactor heating= 12.56, cooling = 7.48 and the total value of  required for whole sterilization process is 52, where  is the design criteria.

What is the holding period (min) at a k value of 3.36min^-1?

QUESTION ID:56


QUESTION ID:57

The Headmaster ___________ to speak to you.
Which of the following options is incorrect to complete the above sentence?

QUESTION ID:58

Mahatama Gandhi was known for his humility as

QUESTION ID:59


Which of the above underlined parts of the sentence is not appropriate?

QUESTION ID:60

Select the pair that best expresses a relationship similar to that expressed in the pair:
water: pipe::

QUESTION ID:61

Velocity of an object fired directly in upward direction is given by V =  80 - 32 t, where t (time) is in seconds. When will the velocity be between 32 m/sec and 64 m/sec?

QUESTION ID:62

In a factory, two machines M1 and M2 manufacture 60% and 40% of the autocomponents respectively. Out of the total production, 2% of M1 and 3% of M2 are found to be defective. If a randomly drawn autocomponent from the combined lot is found defective, what is the probability that it was manufactured by M2?

QUESTION ID:63

Following table gives data on tourists from different countries visiting India in the year 2011.

Which two countries contributed to the one third of the total number of tourists who visited India in 2011?

QUESTION ID:64

If |-2x | + 9 | = 3 then the possible value of |-x | - x^2 would be:

QUESTION ID:65

All professors are researchers
Some scientists are professors
Which of the given conclusions is logically valid and is inferred from the above arguments: