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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Which one of the following complement proteins is the initiator of the membrane attack complex?

QUESTION ID:2

Levinthars paradox is related to 

QUESTION ID:3

Which one of the following is a second generation genetically engineered crop? 

QUESTION ID:4

Based on the heavy chain, which one of the following antibodies has multiple subtypes? 

QUESTION ID:5

The cytokinetic organelle in plant cells is

QUESTION ID:6

Anergy refers to 

QUESTION ID:7

ABO blood group antigens in humans are differentiated from each other on the basis of 

QUESTION ID:8

Which one of the following organises is used for the determination of phenol coefficient of a disinfectant? 

QUESTION ID:9

A single subunit enzyme convects 420 of substrate to product in one minute. The activity of the enzyme is x 10^-6  Katal

QUESTION ID:10

Which one of the following amino acids has the highest probability to be found on the surface of a typical globular protein in aqueous environment? 

QUESTION ID:11

Which one of the following is NOT a product of denitrification in Pseudomonas? 

QUESTION ID:12

The determinant of the matrix  

QUESTION ID:13

Which one of the following features is NOT required in a prokaryotic expression vector? 

QUESTION ID:14

Production of monoclonal antibodies by hybxidoma technology requires 

QUESTION ID:15

Which one of the following is INCORRECT about a typical apoptotic cell?

QUESTION ID:16

Identify the file format given belovr. 
>P1: JMFD
Protein X —Homo sapiens
MKALTARQQEVFDLIRDHISRTLRQQGDWL

QUESTION ID:17

Which one of the following relations holds true for the specific growth rate (µ) of a microorganism in the death phase? 

QUESTION ID:18

How many 3-tuples are possible for the following amino acid sequence? MADCMWDISEASE 

QUESTION ID:19

How many different protein sequences of 100 residues can be generated using 20 standard amino acids? 

QUESTION ID:20

In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be 

QUESTION ID:21

Which one of the follousne aaphs represents uncompetitive inhibition?


QUESTION ID:22

Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR). 

QUESTION ID:23

Consider the following infinite series: If r = 0 3. then the sum of this infinite series is  

QUESTION ID:24

The system of linear equations in two variables shown above will have infinite solutions. if and only if, b is equal to  

QUESTION ID:25

The interaction between an antigen (Ag) and a single-chain antibody (Ab) was studied using Scatchard analysis. The result is shown below.

The affinity of interaction and the total concentration of antibody. respectively. can be determined from 

QUESTION ID:26

An isolated population on an island has the following genotypic frequencies: 
Assuming that there are only two alleles (A and a) for the gene, the genotypic frequency of AA in the next generation will be  

QUESTION ID:27

How many rooted and unmated phylogenetic trees, respectively. are possible with four different sequences? 

QUESTION ID:28

Match the compounds in Group I with the correct entries in Group II


QUESTION ID:29

What are the eigenvalues of the following matrix? 

QUESTION ID:30

For a discrete random variable and the cumulative probability F(X) is shown below: 

 
The mean value of X is  

QUESTION ID:31

March the drugs in Group I with their mechanism of action in Group II. 


QUESTION ID:32

The limit of the function 


QUESTION ID:33

Match the cells in Group I with their corresponding entries in Group II


QUESTION ID:34

Oxygen transfer was measured in a stirred tank bicreactor using dynamic method. The dissolved oxygen tension was found to be 80% air saturatica under steady state conditions. The measured oxygen tensions at 7 s and 17 s were 55% and 68% air saturation, respectively. The volumetrict  mass transfer coefficient

QUESTION ID:35

Match the microorganisms in Group I with their fermentation products in Group II

QUESTION ID:36

Plasmid DNA (0.5 Âµg) containing an ampicillin resistance marker was added to 200 Âµ1 of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 Âµl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained The transfornuttion efficiency is    x 10^6 cfµg^-1

QUESTION ID:37

Match the reagents in Group I with their preferred cleavage sites in Group II 

QUESTION ID:38

Saccharonores cerevisiae produces ethanol by fermentation. The theoretical yield of ethanol from 2.5 g of glucose is ........ g

QUESTION ID:39

Choose the CORRECT sequence of steps involved in cytoplast production.

QUESTION ID:40

Match the antibiotics in Group I with their modes of action in Group II

QUESTION ID:41

The diameters of a large and a small vessel are 1.62 m and 16.2 cm. respectively. The vessels are geometrically similar and operated under similar volumetric agitated power input. The mixing time in the small vessel was found to be 15 s. Determine the mixing time (in seconds) in the large vessel. 

QUESTION ID:42


QUESTION ID:43

Consider the following multiple sequence alignment of four DNA sequences. 

Shannon's entropy of the above alignment is 

QUESTION ID:44

The Kt of a novel competitive inhibitor designed against an enzyme is 2.5M. The enzyme was assayed in the absence or presence of the inhibitor (5 M) under identical conditions. The Km in the presence of the inhibitor was found to be 30 .M. The Km in the absence of the inhibitor is

QUESTION ID:45

A heterozygous tall plant (Tt) was crossed with a homozygous dwarf plant (tt). The resultant seeds were collected. If five seeds are chosen at random then the probability (in %) that exactly two of these seeds will yield dwarf plants is  

QUESTION ID:46

Assuming random distribution of nucleotides. the average number of fragments generated upon digestion of a circular DNA of size 4.3 x 10^5 bp with

QUESTION ID:47

A synchronous culture containing 1.8 x 10^5 monkey kidney cells was seeded into three identical flasks. The doubling time of these cells is 24 h. After 24 h, the cells from all the three flasks were pooled and dispensed equally into each well of three 6-well plates. The number of cells in each well will be ........x 10^4.

QUESTION ID:48

An in vitro translation system can synthesize peptides in all three reading frames of the RNA template. When 5' - UCUCUCUC--(UC).--UCUCUCUC - 3' was used as the template in this in vitro translation system, the synthesized peptides contained 50% each of striae and lencine. When 5' - CCUCCUCCU—(CCU),-CCUCCU - 3' was used as the template, the synthesized peptides contained 33.3% each of striae, leucine, and proline. Deduce the codon for proline. 

QUESTION ID:49

Three distinct antigens X. Y and Z were used to raise antibodies. Antigen Z was injected in a mouse on day zero followed by the administration of antigens X and Y on day 28. A second injection of antigen X was administered on day 70. The antibody titers were monitored in the serum every day and the results are shown below. 
Which one of the following statements regarding the antibody titers in the serum is INCORRECT? 

QUESTION ID:50

The standard free energy change (AG') for ATP hydrolysis is -30 kJ•mole^-1. The in vivo concentrations of ATP. ADP and Pi in .E coli are 7.90. 1.04 and 7.90 mM. respectively. When E. coli cells are cultured at 37 °C. the free energy change (AG)  for ATP hydrolysis in vivo is 

QUESTION ID:51

In a fed-batch culture. 200 g ^-1 glucose solution is added at a flow rate of SO L•h ^-1. The initial culture volume (at quasi steady state) and the initial cell concentration are 600 L and 20 g ^-1. respectively. The yield coefficient (Yx/s) is 0.5 g cell masst substrate^-1. The cell concentration (g • L^-1) at quasi steady state at t=8 h is

QUESTION ID:52

Cytoplasmic extract from the wild type strain of a bacterium has the ability to convert a colorless substrate (S) to a colored product (P) via three colorless intermediates X. Y and Z. in that order. Each step of the pathway involves a specific enzyme coded by a distinct gene. Four mutant strains (a^-, b.^-c^-; d^-) were isolated whose extracts are incapable of producing the colored product in the presence of S. In a series of experiments, extracts from the individual mutants were incubated with 7C, Y. or Z and scored for color development. The data are summarized in the table below. (Yes: color developed, No: no color developed) 
 
Based on the data. which one of the following is the correct order of enzymes involved in the pathway? 

QUESTION ID:53

Biomass is being produced in a continuous stirred tank bioreactor of 750 L capacity. The sterile feed containing 8 g • L^-1 glucose as substrate was fed at a flow rate of 150 L • h^-1. The microbial system follows Monad's model with = 0.4 h ^-1. Ks = 1.5 g • ^.-1 and = 0.5 g cell mass•g substrate-1. Determine the cell productivity (g • L^-1 • h-1) at steady state. 

QUESTION ID:54

A linear double stranded DNA of length 8 kbp has three restriction sites. Each of these can either be a BamHI or a Haelll site. The DNA was digested completely with both enzymes. The products were purified and subjected to an end-filling reaction using the Elena fragment and. The products of the end-filling reaction were purified, resolved by electrophoresis. stained with ethidium bromide (EtBr) and then subjected to autoradiography. The corresponding images are shown below. 
The numbers below each band in the sample lane in the ant:radiograph represent their mean signal intensity in arbitrary units. Which one of the following options is the correct restriction map of the DNA? 

QUESTION ID:55

Samples of bacterial culture taken at 5 PM and then the next day at 5 AM were found to have 10^4 and 10^7 cells-mL^-1. respectively. Assuming that both the samples were taken during the log phase of cell growth. the generation time of this bacterium will be ..........h

QUESTION ID:56

Choose the most appropnate word from the options given below to complete the following sentence. 
The principal presented the chief guest with a . ....... as token of appreciation

QUESTION ID:57

Choose the appropriate word/phrase. out of the four options given below, to complete the following sentence: 
Frogs  

QUESTION ID:58

Choose the word most similar in meaning to the given word: 
Educe 

QUESTION ID:59


QUESTION ID:60

If logx (5/7) = -1/3, then the value of x is 

QUESTION ID:61

The following question presents a sentence, part of which is underlined Beneath the sentence you find four ways of phrasing the underlined part. Following the requirements of the standard written English. select the answer that produces the most effective sentence. 
Tuberculosis. together with its effects, ranks one of the leading causes of death in India. 

QUESTION ID:62

Read the following paragraph and choose the correct statement.

Climate change has reduced human security and threatened human well being. An ignored reality of human progress is that human security largely depends won environmental security. But on the contrary, human progress seems contradictory to environmental security. To keep up both at the required level is a challenge to be addressed by one and all One of the ways to curb the climate change may be suitable scientific innovations. Atit the other may be the Gandhian perspective on small scale progress with focus on sustainability.

QUESTION ID:63

Fill in the missing value 


QUESTION ID:64

A cube of side 3 units is formed using a set of smaller cubes of side 1 unit. Find the proportion of the number of faces of the smaller cubes visible to those which are NOT visible. 

QUESTION ID:65

Humpty Dumpty sits on a wall every day while having lunch. The wall sometimes breaks. A person sitting on the wall falls if the wall breaks. 
Which one of the statements below is logically valid and can be inferred from the above sentences?

QUESTION ID:66

The Kt of a novel competitive inhibitor designed against an enzyme is 2.5M. The enzyme was assayed in the absence or presence of the inhibitor (5 M) under identical conditions. The Km in the presence of the inhibitor was found to be 30 .M. The Km in the absence of the inhibitor is