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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Corona virus genome consists of

QUESTION ID:2

The enzyme that transcribes the eukaryotic genes encoding precursor ribosomalRNAs (pre-rRNAs) of 28S, 18S and 5.8S rRNAs is

QUESTION ID:3

Number of unrooted trees in a phylogeny of five sequences is

QUESTION ID:4

Which one of the following methods is used to test the significance of a predictedphylogeny?

QUESTION ID:5

The Cartesian coordinates (x, y) of a point A with polar coordinates is

QUESTION ID:6

The order of genes present in a chromosome is as follows.


Which one of the following reanangements represents a paracentric inversion?

QUESTION ID:7

Which one of the following statements is INCORRECT about hybridoma production?

QUESTION ID:8

 is equalto

QUESTION ID:9

Which one of the following techniques/tools is NOT used for inserting a foreign gene into a cell?

QUESTION ID:10

Under standard temperature ( T) and pressure ( P) conditions, 128 g of an ideal gas molecule A occupies a volume of 1 L . The gas molecule A obeys the relationship RT = 0.25PV . R and V are universal gas constant and ideal gas volume, respectively. The molecule A is

QUESTION ID:11

CRISPR-Cas system is associated with

QUESTION ID:12

The process by which intracellular macromolecules are supplied for lysosomal degradation during nutrient starvation is

QUESTION ID:13

The process and instrumentation diagram for a feedback control strategy to maintain the level (h) of a liquid by regulating a valve (V) in a tank is shown below. F1 is inlet liquid flow rate, F2 is outlet liquid flow rate, LT is the liquid level transmitter, LC is the liquid level controller, hsp is the setpoint value of the liquid level, hm is the measured value of the liquid level and Pv is the valve pressure.
The manipulating variable(s) is/are

QUESTION ID:14

A protein without its prosthetic group is known as

QUESTION ID:15

The enzyme which adds phosphate group to the free 5' terminus of a DNA sequence is

QUESTION ID:16

Which one of the following is CORRECT about microbial growth medium?

QUESTION ID:17

The cellular process which utilizes RNA-induced silencing complex to block gene expression is

QUESTION ID:18

Which of the following layer(s) is/are formed from the inner cell mass of the blastocyst?

QUESTION ID:19

Which of the following cell organelle(s) is/are surrounded by a single phospholipid membrane?

QUESTION ID:20

The sum of the infinite geometric series (rounded off to one decimal place) is ___ _

QUESTION ID:21

Three balls, colored in blue, green and red, are successively transferred from box A to box B in the order BLUE-GREEN-RED. The probability of a reverse transfer of the balls to the box A in the same order (rounded off to two decimal places)

QUESTION ID:22

Decimal reduction time of a bacterial strain is 20 min. Specific death rate constant in min^-1 (rounded off to two decimal places) is ___ _

QUESTION ID:23

The value of (rounded off to two decimal places) is ____

QUESTION ID:24

A system consists of two reactors, connected by a valve. The first reactor ( R1) contains an ideal gas A of volume 5 L and the second reactor ( R2) has an ideal gas B of volume 10 L . Initially, the valve is closed and pressure P in R1 and reaches equilibrium. If the temperature T of both the reactors is maintained constant, the final equilibrium pressure in atm of the system is ___ _

QUESTION ID:25

The enzyme a-amylase used in starch hydrolysis has an affinity constant ( Km) value of 0.005 M . To achieve one-fourth of the maximum rate of hydrolysis, the required starch concentration in mM (rounded off to two decimal places) is ----

QUESTION ID:26

Which one of the following represents non-growth associated product formation kinetics in a bioprocess system? X and P denote viable cell and product concentrations, respectively.

QUESTION ID:27

Match enzymes in Group I with their corresponding industrial application in Group II.

QUESTION ID:28

Match separation methods in Group I with associated properties in Group II

QUESTION ID:29

Match the autoimmune diseases in Group I with the corresponding primarily affected organ in Group II

QUESTION ID:30

Match hypersensitivity types in Group I with their corresponding condition in Group II.

QUESTION ID:31

Which of the following combinations of plant hormones and their associated function are CORRECT?

QUESTION ID:32

Which one of the following tools is used to compare all the possible six-open reading frames of a given nucleotide query sequence with all the available six-open reading frames of the nucleotide sequence database?

QUESTION ID:33

In Neurospora crassa, a mutation in the poh.y gene results in a slow growth phenotype (poky). The results of four crosses are given below.

Which one of the following explains the inheritance mode of poky?

QUESTION ID:34

Tertiary structure of a protein consisting of a-helices and P-strands can be determined by

QUESTION ID:35

Which of the following statement(s) is/are CORRECT about Agrobacterium tumefaciens?

QUESTION ID:36

Which ofthe following antimicrobial agent(s) is/are growth factor analog(s)?

QUESTION ID:37

Which of the following chemical messenger(s) is/are derivative(s) of tryptophan?

QUESTION ID:38

Which of the following nucleus/nuclei is/are NMR active?

QUESTION ID:39

In a Mendel's dihybrid experiment, a homozygous pea plant with round yellow seeds was crossed with a homozygous plant with wrinkled green seeds.
F; intercross produced 560 F; progeny. The number of F2 progeny having both dominant traits (round and yellow) is ___ _

QUESTION ID:40

A 0.1 mL aliquot of a bacteriophage stock having a concentration of  4xl0^9 phages mL^-1 is added to 0.5 mL of E. coli culture having a concentration of 2x 10^8 cells mL^-1 The multiplicity of infection is ____

QUESTION ID:41

If the area of a triangle with the vertices ( k, 0) , ( 2, 0) and ( 0,-2) is 2 square units, the value of k is ____ .

QUESTION ID:42

In a chemostat with a dilution r ate of 0.8 h^-I, the steady state biomass concentration and the specific product formation rate are 8 mol m^-3 and 0.2 (mol p roduct) (mol biomass f 1 h^-I , respectively. The steady state product concentration in mol m ^-3 is ----

QUESTION ID:43

If the values of two random variables (X, Y) are (121, 360) , (242, 364) and(363, 362) , the value of correlation coefficient between X and Y (rounded off to one decimal place) is ____ .

QUESTION ID:44

The determinant of matrix

QUESTION ID:45

It is desired to scale-up a fermentation from lL to 1OOOL vessel by maintaining a constant power-to-volume ratio. The small fermenter is operated at an agitator speed of 300 rotations per minute (rpm) . If the value of scale up factor is 10, agitator speed in rpm  (rounded off to the nearest integer) for the large fermenter is

QUESTION ID:46

The specific growth rate of a mold during exponential phase of its growth in a batch cultivation is 0.15 h^ -1 . If the cell concentration at 30 h is 33 gL-^1 , the cell concentration in g L^-1 (rounded off to the nearest integer) at 24 h is

QUESTION ID:47

A sedimentation tank of height 100 em is used in a conventional activated sludge process to separate a suspension of spherical shaped granular sludge biomass of 0.5 mm diameter. The viscosity of the liquid is 1 cP. The difference in density between the suspended biomass and the liquid is 0.lgcm^-3 • If the biomass reach their terminal velocity instantaneously, the biomass settling time in min (rounded offto two decimal places) is ____

QUESTION ID:48

In a random mating population, Y and y are dominant and recessive alleles, respectively. If the frequency of Y allele in both sperm and egg is 0.70, then the frequency of Y / y heterozygotes (rounded off to two decimal places) is ____

QUESTION ID:49

Calculate the following integral


QUESTION ID:50

A feed stream ( F1) containing components A and B is processed in a system comprising of separation unit and a mixer as shown below in the schematic diagram. The mole fractions of the components A and B are x A and x8 , respectively. If F1. + F2 = 100 kg h^-1 , the degrees of freedom of the system is ----

QUESTION ID:51

A batch cultivation of E. coli follows zeroth order Monod's growth kinetics. The cell growth is terminated when the residual dissolved oxygen concentration attains 10% of its saturation value and oxygen mass transfer coefficient ( kLa) reaches its maximum value ( 80 h^-1 ) . The saturation value of dissolved oxygen concentration is 0.007 kg m ^-3 • If the maximum specific growth rate and yield coefficient ( Yx/o2 ) are 0.2 h^-1 and 1.5 (kgcells)(kg02)^-1, respectively, then the fmal cell concentration in kg m^-3 (rounded off to two decimal places) at the end of the batch cultivation is

QUESTION ID:52

Milk flowing through a stainless steel inner tube ( 40 mm inner diameter) of double tube-type heater is to be heated from 10 °C to 85 °C by saturated steam condensing at 120 ° Con the outer surface of the inner tube. Total heat transferred (Q) is 146200 kcal h^-1 and the overall heat transfer coefficient is 750 kcal h^-1 m^-2 o c^-1 . The total length of the heating tube in m (rounded off to one decimal place) is

QUESTION ID:53

A DNA solution ofconcentration gives an absorbance of 1.0 at 260nm. An aliquot of purified plasmid solution is diluted with distilled water to a total volume of The diluted plasmid solution gives an absorbance of 0.550 at 260nm. The concentration of the purified plasmid in (rounded off to two decimal places) is ___

QUESTION ID:54

The possible number of Sall restriction sites in a 9 kb double-stranded DNA, with all four bases occurring in equal proportion (rounded off to the nearest integer) is ----

QUESTION ID:55

A bacterium produces acetic acid from ethanol as per the following reaction

The thermodynamic maximum yield of acetic acid from ethanol in g g^-1 (rounded off to two decimal places) is ___ _

QUESTION ID:56

The ratio of boys to girls in a class is 7 to 3.
Among the options below, an acceptable value for the total number of students in the class is:

QUESTION ID:57

A polygon is convex if, for every pair of points, P and Q belonging to the polygon, the line segment PQ lies completely inside or on the polygon. Which one of the following is 1:iQ! a convex polygon?

QUESTION ID:58

Consider the following sentences:
(i) Everybody in the class is prepared for the exam.
(ii) Babu invited Danish to his home because he enjoys playing chess.
Which of the following is the CORRECT observation about the above two sentences?

QUESTION ID:59

A circular sheet of paper is folded along the lines in the directions shown. The paper, after being punched in the final folded state as shown and unfolded in the reverse order of folding, will look like ___ .

QUESTION ID:60

__ is to surgery as writer is to __ Which one of the following options maintains a similar logical relation in the above sentence?

QUESTION ID:61

We have 2 rectangular sheets of paper, M and N, of dimensions 6 em x 1 em each. Sheet M is rolled to form an open cylinder by bringing the short edges of the sheet together. Sheet N is cut into equal square patches and assembled to form the largest possible closed cube. Assuming the ends of the cylinder are closed, the ratio of the volume of the cylinder to that of the cube is

QUESTION ID:62

Details of prices of two items P and Q are presented in the above table. The ratio of cost of item P to cost of item Q is 3:4. Discooot is calculated as the difference between the marked price and the selling price. The profit percentage is calculated as the ratio of the difference between selling price and cost, to the cost


The discooot on item Q, as a. percentage of its marked price, is

QUESTION ID:63

There are five bags each containing identical sets of ten distinct chocolates. One chocolate is picked from each bag.
The probability that at least two chocolates are identical is

QUESTION ID:64

Given below are two statements 1 and 2, and two conclusions I and II.
Statement 1: All bacteria are microorganisms.
Statement 2: All pathogens are microorganisms.
Conclusion 1: Some pathogens are bacteria.
Conclusion II: All pathogens are not bacteria.
Based on the above statements and conclusions, which one of the following options is logically CORRECT?

QUESTION ID:65

Some people suggest anti-obesity measures (AOM) such as displaying calorie information in restaurant menus. Such measures sidestep addressing the core problems that cause obesity: poverty and income
inequality. Which one of the following statements summarizes the passage?