Menu

Previous Year Questions

Performance Meter

0%

TLS Online TPP Program

QUESTION ID:1

Measurement of the absorbance of a solution containing NADH in a path length of 1cm cuvette at 340 nm shows the value of 0.31. The molar extinction coefficient of NADH is 6200 M-1 cm-1. The concentration of NADH in the solution is ___ μM (correct to integer number).

QUESTION ID:2

Among the reagents given below which one of the combination of reagents will NOT break the disulphide bonds in the immunoglobulin molecules?
(P) Reduced glutathione (Q) Dithiothritol
(R) Sodium dodecyl sulphate (S) Methionine

QUESTION ID:3

Match the protein elution condition given in Group I with the appropriate chromatography matrices from Group II.

QUESTION ID:4

Which one of the following is NOT a neurotransmitter?

QUESTION ID:5

The type-II hypersensitivity reaction is mainly mediated by____.

QUESTION ID:6

Which one the following reaction mechanisms drives the conversion of low energy 3- phosphoglyceraldehyde to high energy 1,3-bisphosphoglycerate?

QUESTION ID:7

A polymerase reaction is carried out for 10 cycles in a volume of 1 ml with 5 molecules of template DNA. Assuming that the efficiency of the reaction is 100 %, the number of molecules of DNA present in 100 -l at the end of the reaction is ___ (correct to integer number).

QUESTION ID:8

The secondary structure topology diagram of 400 amino acid long “Protein-X” is depicted in the figure. The start and end amino acid residue numbers of each Alpha -helix are marked. The percentage (correct to integer number) of residues forming Alpha-helix is____.

QUESTION ID:9

An enzyme follows Michaelis-Menten kinetics with substrate S. The fraction of the maximum velocity (Vmax) will be observed with the substrate concentration [S] = 4Km is _____ (correct to one decimal place). (Km is Michaelis-Menten constant)

QUESTION ID:10

The mass spectrum of benzoic acid will generate the fragment as a base peak (100% relative abundance) of m/z (mass to charge ratio) at ____ (correct to integer number).

QUESTION ID:11

The standard free energy (ΔG´) values of reactions catalyzed by citrate lyase and citrate synthetase are -670 and -8192 cal/mol, respectively.

The standard free energy (in cal/mol) of acetyl-CoA hydrolysis is ___ (correct to integer number).

QUESTION ID:12

Which of the following genera produces dimorphic seeds that help to broaden the time of germination in a variable habitat?

QUESTION ID:13

The genes for microRNA (miRNA) in plants are usually transcribed by

QUESTION ID:14

Which of the statements is TRUE for transposable elements Ac and Ds?

QUESTION ID:15

Identify the CORRECT statement.

QUESTION ID:16

Which of the following statements is TRUE for an ecotone?

QUESTION ID:17

Acid rain with a pH of 4.0 is more acidic than the rain with a pH of 6.0 by

QUESTION ID:18

Which of the following plants produces Ylang-ylang oil?

QUESTION ID:19

Identify the INCORRECT statement in connection with polar transport of auxin.

QUESTION ID:20

Which of the following stains is used to visualize callose under the microscope?

QUESTION ID:21

The coding sequence of a gene XLR18 has the single ORF of 783 bp. The approximate molecular weight of the XLR18 protein in kDa is ______.

QUESTION ID:22

Statements given below are either TRUE (T) or FALSE (F). Select the CORRECT combination.
P. Mitosis occurs exclusively in diploid mother cell
Q. Mitosis occurs both in diploid and haploid mother cells
R. Meiosis occurs exclusively in diploid mother cell
S. Meiosis occurs both in diploid and haploid mother cells

QUESTION ID:23

You are asked to design a genetic construct for high-level expression of a gene encoding the therapeutic protein 18 (TP18) via plastid transformation. Select the CORRECT set of genetic elements for this construct.

QUESTION ID:24

Select the CORRECT combination of the following statements.
P. The cyclic electron transport chain involving PSI results in net production of both ATP and NADPH
Q. The cyclic electron transport chain involving PSI results in net production of ATP
R. Rubisco enzyme usually converts RuBP and CO2 into 2-phosphoglycolate and 3-phosphoglycerate
S. Rubisco enzyme usually converts RuBP and O2 into 2-phosphoglycolate and 3-phosphoglycerate

QUESTION ID:25

Match the fruit characters with their families and representative plant species.

QUESTION ID:26

Select the CORRECT combination by matching the disease, affected plant and the causal organism.

QUESTION ID:27

Select the CORRECT combination by matching Group-I with Group-II.

QUESTION ID:28

Match the plant alkaloids with their uses and source species.

QUESTION ID:29

Identify the CORRECT combination of statements with respect to chemical defense in plants.
P. Pisatin, a phytoalexin produced by Ricinus communis is a constitutive defense compound
Q. Phaseolus vulgaris produces Phaseolus agglutinin I, which is toxic to the cowpea weevil
R. A single step non-enzymatic hydrolysis of cyanogenic glycoside releases the toxic hydrocyanic acid (HCN) to protect plant against herbivores and pathogens
S. Avenacin, a triterpenoid saponin from oat prevents infection by Gaeumannomyces graminis, a major pathogen of cereal roots

QUESTION ID:30

In garden pea, dwarf plants with terminal flowers are recessive to tall plants with axial flowers. A true-breeding tall plant with axial flowers was crossed with a true-breeding dwarf plant with terminal flowers. The resulting F1 plants were testcrossed, and the following progeny were obtained:
Tall plants with axial flowers = 320
Dwarf plants with terminal flowers = 318
Tall plants with terminal flowers = 79
Dwarf plants with axial flowers = 83
The map distance between the genes for plant height and flower position is______cM.

QUESTION ID:31

Two true-breeding snapdragon (Antirrhinum majus) plants, one with red flowers and another with white flowers were crossed. The F1 plants were all with pink flowers. When the F1 plants were selfed, they produced three kinds of F2 plants with red, pink and white flowers in a 1:2:1 ratio. The probability that out of the five plants picked up randomly, two would be with pink flowers, two with white flowers and one with red flowers is _____%.

QUESTION ID:32

David Baltimore’s classification of viruses is based on differences in

QUESTION ID:33

Which of the following immune system components can function as an opsonin?

QUESTION ID:34

The oral polio vaccine (OPV) consists of

QUESTION ID:35

Which of the following eukaryotic cellular components carries out intracellular degradation during autophagy?

QUESTION ID:36

Analysis of DNA sequences suggest that eukaryotic mitochondrial genomes primarily originated from

QUESTION ID:37

Binomial nomenclature has NOT yet been adopted for

QUESTION ID:38

Which of the following is NOT an accepted method for sterilization?

QUESTION ID:39

The primary product of nitrogen fixation is

QUESTION ID:40

In humans, the key stages in the life cycle of malarial parasites occur in

QUESTION ID:41

You have a 50 mg/mL stock solution of arginine. To prepare 1 liter of growth medium for an arginine auxotroph that requires 70 μg/mL of arginine, the volume of this stock solution that should be added is _____________ mL (up to 1 decimal point) .

QUESTION ID:42

Accumulating evidence suggest that Domain Archaea is more closely related to Domain Eukarya than to Domain Bacteria. Which of the following properties are shared between eukaryotes and archaea ?
(i) Protein biogenesis
(ii) Presence of sterol containing membranes
(iii) Ribosomal subunit structures
(iv) Adaptation to extreme environmental conditions
(v) Fatty acids with ester linkages in the cell membrane

QUESTION ID:43

Match the antimicrobial agents in group I with their category/mode of action in group II.

QUESTION ID:44

Match the microorganisms to their predominant modes of transmission.

QUESTION ID:45

Match the precursors/intermediates with the corresponding metabolic pathways.

QUESTION ID:46

Match the scientists to their area of major contribution

QUESTION ID:47

Which of the following combinations would improve the resolution of a microscope?
(i) Increasing the half aperture angle of the objective lens
(ii) Decreasing the wavelength of the illumination source
(iii) Decreasing the numerical aperture of the objective lens
(iv) Decreasing the refractive index of immersion medium

QUESTION ID:48

Active transport involves the movement of a biomolecule against a concentration gradient across the cell membrane using metabolic energy. If the extracellular concentration of a biomolecule is 0.005M and its intracellular concentration is 0.5M, the least amount of energy that the cell would need to spend to transport this biomolecule from the outside to the inside of the cell is ________ kcal/mol (up to 2 decimal points). (Temperature T = 298K and universal gas constant R= 1.98 cal/mol.K)

QUESTION ID:49

A continuous cell culture being carried out in a stirred tank reactor is described in terms of its cell mass concentration X and substrate concentration S. The concentration of the substrate in the sterile feed stream is SF = 10 g/L and yield coefficient Yx/s = 0.5. The flow rates of the feed stream and the exit stream are equal (F=5 mL/min) and constant. If the specific growth rate (h-1) 𝜇 = 0.3 𝑆/(1+𝑆), the steady state concentration of S is _____ g/L (up to 1 decimal point).

QUESTION ID:50

The initial concentration of cells (N0) growing unrestricted in a culture is 1.0×106 cells/mL. If the specific growth rate (μ) of the cells is 0.1 h-1, the time required for the cell
concentration to become 1.0×108 cells/mL is _________ hours (up to 2 decimal points).

QUESTION ID:51

The following stoichiometric equation represents the conversion of glucose to lactic acid in a cell:

If the free energy of conversion of glucose to lactic acid only is ΔG0 = -47000 cal/mol, the efficiency of energy transfer is _______ % (up to 1 decimal point). (ΔG0 for ATP hydrolysis is -7.3 kcal/mol)

QUESTION ID:52

Animals belonging to phylum Echinodermata are closer to chordates than other invertebrate phyla. Which ONE of the following reasons can account for this relatedness?

QUESTION ID:53

A zoologist recovered some tissue from preserved skin of a woolly mammoth. Further genetic analysis requires DNA isolation and increasing its amount. Which ONE of the following techniques would be most useful for increasing the amount of DNA?

QUESTION ID:54

In a chemical reaction where the substrate and product are in equilibrium in solution, what will occur if an enzyme is added?

QUESTION ID:55

Tay-Sachs disease is a human genetic disorder that is associated with defects in which ONE of the following cellular organelles?

QUESTION ID:56

Increase in the existent population of grey peppered moth, Biston betularia, during industrial revolution in Britain is an example of which ONE of the following evolutionary processes?

QUESTION ID:57

Which ONE of the following is NOT a characteristic of a cancer cell? 

QUESTION ID:58

Cardiac and cerebral tissues are derived from the following germ layers respectively

QUESTION ID:59

An animal’s ability to escape from a predator by using the explored knowledge of home area is an example of

QUESTION ID:60

Bowman’s capsules are present in which ONE of the following organs/ tissues?

QUESTION ID:61

Which ONE of the following is the primary function of lung surfactants?

QUESTION ID:62

Match the disorders/diseases listed in Column I to their respective causative agents listed in Column II

QUESTION ID:63

Glucose monomers are joined together by glycosidic linkages to form a cellulose polymer. During this process, changes in the free energy, total energy, and entropy respectively are represented correctly by which ONE of the following options?

QUESTION ID:64

In Drosophila melanogaster, a mutation in Ultrabithorax which defines the third segment of the thorax or T3 leads to development of four winged flies, as the halteres develop into a second pair of wings. Which ONE of the following phenotypes in fly will result from overexpression of Ultrabithorax in the second thoracic segment?

QUESTION ID:65

Which ONE of the following is TRUE in case of respiratory acidosis?

QUESTION ID:66

Match the proteins / molecules listed in column I with the cellular location mentioned in the column II.

QUESTION ID:67

In an experiment, nucleus from a Drosophila oocyte was transplanted into the anterior part of another oocyte, at a region opposite to the existing nucleus. Which ONE of the following phenotypes will the developing egg show?

QUESTION ID:68

Match the organisms listed in Column I with the features listed in Column II

QUESTION ID:69

Which ONE of the following statements is NOT part of the classical Darwinian theory of evolution by natural selection?

QUESTION ID:70

A population of rabbits was determined to have a birth rate of 200 and mortality rate of 50 per year. If the initial population size is 4000 individuals, after 2 years of non-interfered breeding the final population size will be _______________.

QUESTION ID:71

In a population which is in Hardy-Weinberg equilibrium, the frequency of occurrence of a disorder caused by recessive allele (q) is 1 in 1100. The frequency of heterozygotes in the population will be ______________. (Give the answer to three decimal places).

QUESTION ID:72

Which of the following is an oil soluble pigment present in fruits and vegetables?

QUESTION ID:73

Which of the following represent the group of saturated fatty acids?

QUESTION ID:74

The anti-nutritional factor present in fava bean is

QUESTION ID:75

Which of the following is a Gram positive bacteria?

QUESTION ID:76

Irradiation carried out to reduce viable non-spore forming pathogenic bacteria using a dose between 3 to 10 kGy is

QUESTION ID:77

Identify the correct statement related to the viscosity of Newtonian fluids from the following.

QUESTION ID:78

Adult male Wistar rats were fed with a protein based diet. Total 150 g of protein was ingested per animal. If the average weight increased from 110 g to 350 g after the end of experiment, the Protein efficiency ratio of the given protein would be _______. (up to two decimal points).

QUESTION ID:79

The initial moisture content of a food on wet basis is 50.76%. Its moisture content (%) on dry basis is _______.(up to two decimal points)

QUESTION ID:80

The oxygen transmission rate through a 2.54 x 10-3 cm thick low density polyethylene film with air on one side and inert gas on the other side is 3.5 x 10-6 mL cm-2 s-1. Oxygen partial pressure difference across the film is 0.21 atm. The permeability coefficient of the film to oxygen is _______ x 10-11 mL (STP) cm cm-2 s-1 (cm Hg)-1.

QUESTION ID:81

Ambient air at 30◦C dry bulb temperature and 80% relative humidity was heated to a dry bulb temperature of 80◦C in a heat exchanger by indirect heating. The amount of moisture gain (g kg-1 dry air) during the process would be _______.

QUESTION ID:82

Match the commodity in Group I with the bioactive constituent in Group II

QUESTION ID:83

Match the process operation in Group I with the separated constituent in Group II

QUESTION ID:84

Match the spoilage symptom in Group I with the causative microorganism in Group II

QUESTION ID:85

Match the fermented product in Group I with the base material in Group II

QUESTION ID:86

Match the operation in Group I with the process in Group II

QUESTION ID:87

Out of 7 principles of HACCP system, 4 are listed below. Arrange these principles in the order in which they are applied.
(P) Conduct a hazard analysis
(Q) Establish monitoring process
(R) Establish critical limit
(S) Establish record keeping and documentation process

QUESTION ID:88

For an enzyme catalyzed reaction S→ P , the kinetic parameters are: [S] = 40 μM, V0 = 9.6 μM s-1 and Vmax = 12.0 μM s-1. The Km of the enzyme in μM will be _______.(up to one decimal points)

QUESTION ID:89

A microbial sample taken at 10 AM contained 1x105 CFU/mL. The count reached to 1x1010 CFU/mL at 8 PM of the same day. The growth rate (h-1) of the microorganism
would be _______.(up to two decimal points)

QUESTION ID:90

The rate of heat transfer per unit area from a metal plate is 1000 W m-2. The surface temperature of the plate is 120◦C and ambient temperature is 20◦C. The convective heat transfer coefficient (W m-2 ◦C-1) using the Newton’s law of cooling will be _______.

QUESTION ID:91

Identify an example of a classical diffusional mass transfer process without involving heat, among the following.