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Auxins in biological samples can be determined by bioassay, mass spectrometry, or enzyme-linked immunosorbent assay (ELISA).
The Avena coleoptile curvature assay is a sensitive measure of auxin activity (it is effective for IAA concentrations of about 0.02 to 0.2 mg L–1).

#Unit 6. System Physiology – Plant #Auxin/GA/Cytokinin #Part B Pointers