Strigolactones as signal that interact with auxin in regulating apical dominance

TLS Online TPP Program

#Id: 4677

#Unit 13. Methods in Biology

A modification of the agglutination reaction, called agglutination inhibition, provides a highly sensitive assay for small quantities of an antigen. For example, one of the early types of home pregnancy test kits included latex particles coated with human chorionic gonadotropin (HCG) and antibody to HCG. 

TLS Online TPP Program

#Id: 4678

#Unit 13. Methods in Biology

Enzyme-linked immunosorbent assay, commonly known as ELISA (or EIA), is similar in principle to RIA but depends on an enzyme rather than a radioactive label. 

TLS Online TPP Program

#Id: 4679

#Unit 13. Methods in Biology

Antigen can be detected or measured by a sandwich ELISA .In this technique, the antibody (rather than the antigen) is immobilized on a microtiter well. 

A sample containing antigen is added and allowed to react with the immobilized antibody. After the well is washed, a second enzyme- linked antibody specific for a different epitope on the antigen is added and allowed to react with the bound antigen

After any free second antibody is removed by washing, substrate is added, and the colored reaction product is measured.

TLS Online TPP Program

#Id: 4680

#Unit 13. Methods in Biology

What are the principles in flow cytometry?

Light scattered by a laser or arc lamp

Specific fluorescence detection

Hydrodynamically focused stream of particles

Electrostatic particle separation for sorting

Multivariate data analysis capability

TLS Online TPP Program

#Id: 4681

#Unit 13. Methods in Biology

3H thymidine uptake assays were the first to be used routinely to measure cell division in lymphocyte cultures.

TLS Online TPP Program

#Id: 4682

#Unit 13. Methods in Biology

Colorimetric Assays for Cell Division Are Rapid and Eliminate the Use of Radioactive Isotopes