Human papillomavirus encodes two oncogenic proteins: E6 -inhibits p53

E7- inhibits RB

#Id: 2840

#Unit 13. Methods in Biology

#Id: 2841

#Unit 13. Methods in Biology

Phosphorylated protein or amino acid are separated by using immobilized metal affinity chromatography ( IMAC), or immuno-affinity using antibodies against phosphorylated amino acids or peptides

#Id: 2842

#Unit 13. Methods in Biology

GLUT separation is also done with the help of affinity chromatography, normally glut protein are cloned into the vector and then the help of affinity chromatography

#Id: 2843

#Unit 13. Methods in Biology

Recovery of proteins using (6His-Tag) and (Ni-NTA) chromatography columns.

#Id: 2844

#Unit 13. Methods in Biology

The glucose uniporter GLUT1 can be purified from the solubilized mixture by antibody affinity chromatography on a column containing either a GLUT1-specific monoclonal antibody or an antibody specific for the epitope tag, then incorporated into liposomes made of pure phospholipids.

#Id: 2845

#Unit 13. Methods in Biology

Affinity chromatography fraction can be analyzed by two ways

Absorption or UV visible spectrophotometry which analyzes the concentration of protein in the fraction by using Beer lamberts law.

Polyacrylamide gel electrophoresis to fractionize the protein sample on the basis of their molecular weight.