A cell is determined to a given fate if it maintains its developmental maturation toward this cell type even when placed in a new environment (commitment is irreversible).

TLS Online TPP Program

#Id: 3117

#Unit 3. Fundamental Processes

The origin of E. coli, oriC, is 245 bp in length. The genome of E. coli is replicated bidirectionally from a single unique site called the origin, identified as the genetic locus oriC.

Two replication forks initiate atoriCand move around the genome at approximately the same speed to a special termination region.

TLS Online TPP Program

#Id: 3118

#Unit 3. Fundamental Processes

A replication fork moves 10X faster than RNA polymerase. 

TLS Online TPP Program

#Id: 3120

#Unit 3. Fundamental Processes

oriC contains binding sites for DnaA: dnaA-boxes.

TLS Online TPP Program

#Id: 3122

#Unit 3. Fundamental Processes

oriC also contains 11 GATC/CTAG repeats that are methylated on adenine on both strands.

TLS Online TPP Program

#Id: 3123

#Unit 3. Fundamental Processes

Replication generates hemimethylated DNA, which cannot initiate replication

TLS Online TPP Program

#Id: 3124

#Unit 3. Fundamental Processes

There is a 13-minute delay before the GATC/CTAG repeats are remethylated.