ACTH (corticotropin) controls the secretion of cortisol by the activation of adenylyl cyclase via Galpha s.

TLS Online TPP Program

#Id: 2615

#Unit 13. Methods in Biology

In plants, the perfect complementarity between most miRNAs and their targets enables the accurate predictions of their targets, while slicing of the targeted mRNAs facilitates target validation through RNA Ligase-Mediated (RLM)-Rapid Amplification of cDNA Ends (RACE) method.

TLS Online TPP Program

#Id: 2616

#Unit 13. Methods in Biology

Type I restriction endonucleases are complex endonucleases and have recognition sequences of about 15 bp; 

TLS Online TPP Program

#Id: 2617

#Unit 13. Methods in Biology

Type I restriction endonucleases cleave the DNA about 1000 bp away from the 5'-end of the sequence "TCA" located within the recognition site.

TLS Online TPP Program

#Id: 2618

#Unit 13. Methods in Biology

Type II restriction endonucleases are remarkably stable and induce cleavage either, in most cases, within or immediately outside their recognition sequences.

TLS Online TPP Program

#Id: 2619

#Unit 13. Methods in Biology

More than 350 different type II endonucleases with over 100 different recognition sequences are known.

TLS Online TPP Program

#Id: 2620

#Unit 13. Methods in Biology

Some restriction endonucleases and their recognition sites.