The polyadenylation reaction passes through two stages.
First, a rather short oligo(A) sequence (10 residues) is added to the 3’ end. This reaction is absolutely dependent on the AAUAAA sequence and poly(A) polymerase performs it under the direction of the specificity factor.
In the second phase, the nuclear poly(A) binding protein (PABP II) binds the oligo(A) tail to allow extension of the poly(A) tail to the full 200 residue length.
#Unit 3. Fundamental Processes#Polyadenylation#Part B Pointers
