THE PUNNETT SQUARE METHOD

TLS Online TPP Program

#Id: 2308

#Unit 3. Fundamental Processes

Sigma factor contacts the promoter directly at both the -35 and -10 consensus sequences was provided by substitutions in the sigma factor that suppressed mutations in the consensus sequences.

TLS Online TPP Program

#Id: 2309

#Unit 3. Fundamental Processes

Comparisons of the sequences of several bacterial sigma factors suggested conserved regions in E. coli s70  that interact directly with promoters DNA-binding regions of the sigma factor fold into independent domains in the protein regions 1.2, 2.3– 2.4, 3.0, and 4.1–4.2.

TLS Online TPP Program

#Id: 2310

#Unit 3. Fundamental Processes

The transition from the closed to the open complex involves structural changes in the enzyme and the opening of the DNA double helix to reveal the template and nontemplate strands. This “melting” occurs between positions –11 and +2, with respect to the transcription start site.

TLS Online TPP Program

#Id: 2311

#Unit 3. Fundamental Processes

P-TEFb activates elongation, splicing and polyadenylation and phosphorylates

  Serine 2 CTD

  TAT-SF1

  SPT5

TLS Online TPP Program

#Id: 2312

#Unit 3. Fundamental Processes

Ser2 phosphatase Fcp1 regenerates RNA Pol II pool

TLS Online TPP Program

#Id: 2313

#Unit 3. Fundamental Processes

Splicing is an SN2 reaction that proceeds through a pentavalent phosphorous intermediate.