Prototrophs– Wild type

TLS Online TPP Program

#Id: 4714

#Unit 13. Methods in Biology

The most useful range for proteins is above 230 nm, where there are absorptions from aromatic side chains While a very weak absorption maximum of phenylalanine occurs at 257 nm, tyrosineand tryptophan dominate the typical protein spectrum with their absorption maxima at 274 nm and 280 nm, respectively

TLS Online TPP Program

#Id: 4715

#Unit 13. Methods in Biology

Cystine (Cys2) possesses a weak absorption maximum of similar strength as phenylalanine at 250 nm. This band can play a role in rare cases in protein optical activity or protein fluorescence.

TLS Online TPP Program

#Id: 4716

#Unit 13. Methods in Biology

Proteins that contain prosthetic groups (e.g. haem, flavin, carotenoid) and some metal–protein complexes, may have strong absorption bands in the UV/Vis range.

TLS Online TPP Program

#Id: 4717

#Unit 13. Methods in Biology

n. Carotenoids, for instance, are a large class of red, yellow and orange plant pigments composed of long carbon chains with many conjugated double bonds. They contain three maxima in the visible region of the electromagnetic spectrum (420 nm, 450 nm, 480 nm).

TLS Online TPP Program

#Id: 4718

#Unit 13. Methods in Biology

Electron delocalisation extends throughout the cyclic tetrapyrrole ring of porphyrins and gives rise to an intense transition at 400 nm called the Soret band.

TLS Online TPP Program

#Id: 4719

#Unit 13. Methods in Biology

Molecules such as FAD (flavin adenine dinucleotide), NADH and NADþ are important coenzymes of proteins involved in electron transfer reactions (RedOx reactions). They can be conveniently assayed by using their UV/Vis absorption: 438 nm (FAD), 340 nm (NADH) and 260 nm (NADþ).