Nurturing Life Sciences
#Id: 2172
#Id: 896
#Unit 13. Methods in Biology
Two REs recognize the same DNA sequence motif but cleave at different positions, then they are called neoschizomers
#Id: 897
Process of replication in which gap is created by DNase I, filled by klenow fragment and with radioactive dNTPs called as Nick Translation
#Id: 898
Terminal transferase (TdT) Adds homopolymer tails to the 3’end of DNA.
#Id: 899
T4 DNA ligase Links 5-phosphate and 3-hydroxyl ends via phosphodiester bond.
#Id: 900
T4 polynucleotide kinase (PNK) Transfers terminal phosphate groups from ATP to 5-OH groups.
#Id: 901
Alkaline phosphatase Removes 5’ phosphates from DNA and RNA.