Thus, although the aNTD is embedded in the body of the enzyme, the aCTD can reach the upstream element and can do so even when that element is not located immediately adjacent to the –35 region, but further upstream.

TLS Online TPP Program

#Id: 733

#Unit 3. Fundamental Processes

DNA methylation in bacteria allows the DNA mismatch recognition machinery to distinguish the old template strand from the new strand.

TLS Online TPP Program

#Id: 734

#Unit 3. Fundamental Processes

Initiation of replication in E.Coli at oriC starts with formation of a complex that ultimately requires six proteins: DnaA, DnaB, DnaC, HU, gyrase, and SSB

TLS Online TPP Program

#Id: 735

#Unit 3. Fundamental Processes

The minimal origin is defined by the distance between the outside members of the 13-mer and 9-mer repeats.

TLS Online TPP Program

#Id: 736

#Unit 3. Fundamental Processes

The four 9-bp consensus sequences on the right side of oriC provide the initial binding sites for DnaA-ATP

TLS Online TPP Program

#Id: 737

#Unit 3. Fundamental Processes

A two-state assembly model explains melting of 13mer during initiation

TLS Online TPP Program

#Id: 738

#Unit 3. Fundamental Processes

To identify DNA that is in the process of replicating, DNA derived from dividing cells is first cut with a restriction enzyme and separated on a 2D agarose gel.