If the middle slice is too narrow, there is no discernible morphogen gradient within it, and regeneration is abnormal.

TLS Online TPP Program

#Id: 4763

#Unit 5. Developmental Biology

SKN-1 also inhibits PAL-1 (preventing it from becoming active in the EMS cell).

TLS Online TPP Program

#Id: 4764

#Unit 5. Developmental Biology

In the germ line, PAL-1 synthesis is prevented by the PUF-8 protein, which binds to the 3′ UTR of pal-1 mRNA and blocks its translation.

TLS Online TPP Program

#Id: 4765

#Unit 5. Developmental Biology

 PIE -1, is necessary for germline cell fate.

TLS Online TPP Program

#Id: 4766

#Unit 5. Developmental Biology

PIE-1 is placed into the P blastomeres through the action of the PAR-1 protein & it inhibit both SKN-1 and PAL-1 function in the P2 and subsequent germline cells.

TLS Online TPP Program

#Id: 4767

#Unit 5. Developmental Biology

Mutations of the maternal pie-1 gene result in germline blastomeres adopting somatic fates, with the P2 cell behaving similarly to a wild-type EMS blastomere.

TLS Online TPP Program

#Id: 4768

#Unit 5. Developmental Biology

In C. elegans, conditional specification occur in the development of the endoderm cell lineage.