TBE gives superior separation of smaller fragments (< 2 kb) in comparison to TAE and is often used for separation of small RNA molecules such as microRNA.

TLS Online TPP Program

#Id: 3533

#Unit 2. Cellular Organization

A functional gene can be located through the use of an expressed sequence tag (EST)

TLS Online TPP Program

#Id: 3534

#Unit 2. Cellular Organization

Animal cells have small mitochondrial genomes 16.6 kb in mammals. There are several hundred mitochondria per cell and each mitochondrion has multiple copies of the DNA.

TLS Online TPP Program

#Id: 3535

#Unit 2. Cellular Organization

Mitochondrial genomes have genes encoding (mostly complex I–IV) proteins, rRNAs, and tRNAs

TLS Online TPP Program

#Id: 3536

#Unit 2. Cellular Organization

Human mitochondrial DNA has 22 tRNA genes, 2 rRNA genes, and 13 protein-coding regions. 

TLS Online TPP Program

#Id: 3537

#Unit 2. Cellular Organization

The fivefold discrepancy in size between the S. cerevisiae (84 kb) and mammalian (16.6 kb) mitochondrial genomes indicates the fact that there must be a great difference in their genetic organization in spite of their common function.

TLS Online TPP Program

#Id: 3538

#Unit 2. Cellular Organization

The chloroplast genome in land plants encodes 4 rRNAs, 30 tRNAs, and 60 proteins.