SDS–PAGE are firstly boiled for 5 min in sample buffer containing - mercaptoethanol or dithiothreitol ( DTT) and SDS. Mercaptoethanol or DTT reduce any disulfide bridges

TLS Online TPP Program

#Id: 3811

#Unit 5. Developmental Biology

Chemotaxis of Dictyostelium myxamoebae is a result of spiral waves of cAMP.

TLS Online TPP Program

#Id: 3817

#Unit 5. Developmental Biology

Neighboring cells respond to cAMP in two ways:

Initiate a movement toward the cAMP pulse for about a minute

Release cAMP of their own

TLS Online TPP Program

#Id: 3820

#Unit 5. Developmental Biology

The top of the mound continues to emit cAMP pulses and is pushed upwards by the movement of cells underneath

TLS Online TPP Program

#Id: 3826

#Unit 5. Developmental Biology

The emerging column of cells topples over called ‘the slug’

24 KDa glycoprotein (gp 24)- Ddcad1

80 KDa glycoprotein (gp 80)- Csa A

150 KDa glycoprotein (gp 150)- Lag C

TLS Online TPP Program

#Id: 3830

#Unit 5. Developmental Biology

Slug migrates to the top layer of the soil, where it proceeds to build the fruiting structure.

TLS Online TPP Program

#Id: 3833

#Unit 5. Developmental Biology

Fruiting structure consists of a tapered column of stalk cells that carries a spherical mass of spores aloft.