.In the Native (Buffer) Gels electrophoresis SDS is absent, and the proteins are not denatured prior to loading. Since all the proteins in the sample being analyzed carry their native charge at the pH of the gel (normally pH 8.7), proteins separate according to their different electrophoretic mobilities and the sieving effects of the gel.

TLS Online TPP Program

#Id: 2318

#Unit 3. Fundamental Processes

Major U2-type or GU-AG introns

TLS Online TPP Program

#Id: 2319

#Unit 3. Fundamental Processes

Splicing signals for minor introns

TLS Online TPP Program

#Id: 2320

#Unit 3. Fundamental Processes

Minor U12-type with GU-AG introns (hybrid type)

TLS Online TPP Program

#Id: 2321

#Unit 3. Fundamental Processes

Splice sites are directional in nature. 5’ splice site -the left, or donor, site and the 3’ splice site - right, or acceptor, site.

TLS Online TPP Program

#Id: 2322

#Unit 3. Fundamental Processes

Splicing complexes

TLS Online TPP Program

#Id: 2323

#Unit 3. Fundamental Processes

Lariat formation is characterize by 2’-5’ phosphodiester bond